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- PDB-6u94: Structure of RND efflux system, outer membrane lipoprotein, NodT ... -
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Open data
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Basic information
Entry | Database: PDB / ID: 6u94 | ||||||
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Title | Structure of RND efflux system, outer membrane lipoprotein, NodT family from Burkholderia mallei ATCC 23344 | ||||||
![]() | RND efflux system, outer membrane lipoprotein, NodT family | ||||||
![]() | LIPID TRANSPORT / outer membrane lipoprotein / NodT family / Structural Genomics / Seattle Structural Genomics Center for Infectious Disease / SSGCID | ||||||
Function / homology | ![]() efflux transmembrane transporter activity / membrane => GO:0016020 / plasma membrane Similarity search - Function | ||||||
Biological species | ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Horanyi, P.S. / Fox III, D. / Abendroth, J. / Lorimer, D. / Edwards, T. / Seattle Structural Genomics Center for Infectious Disease (SSGCID) | ||||||
![]() | ![]() Title: Structure of RND efflux system, outer membrane lipoprotein, NodT family from Burkholderia mallei ATCC 23344 Authors: Horanyi, P.S. / Fox III, D. / Abendroth, J. / Lorimer, D. / Edwards, T. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 170.2 KB | Display | ![]() |
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PDB format | ![]() | 128.9 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 419.7 KB | Display | ![]() |
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Full document | ![]() | 420.8 KB | Display | |
Data in XML | ![]() | 9.3 KB | Display | |
Data in CIF | ![]() | 13 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 3d5kS S: Starting model for refinement |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 63968.863 Da / Num. of mol.: 1 / Fragment: UNP residues 28-619 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() Strain: ATCC 23344 / Gene: BMAA1392 / Production host: ![]() ![]() | ||||
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#2: Chemical | #3: Water | ChemComp-HOH / | Has ligand of interest | N | |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.43 Å3/Da / Density % sol: 49.48 % |
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Crystal grow | Temperature: 298 K / Method: vapor diffusion, sitting drop Details: 30 mM magnesium chloride hexahydrate, 30 mM calcium chloride dihydrate, 0.1 M Tris base, BICINE, pH 8.5, 20% v/v ethylene glycol, 10% w/v PEG8000 |
-Data collection
Diffraction | Mean temperature: 100 K / Serial crystal experiment: N |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: RAYONIX MX-300 / Detector: CCD / Date: Apr 18, 2019 |
Radiation | Monochromator: diamond(111) / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.97856 Å / Relative weight: 1 |
Reflection | Resolution: 2.35→30.48 Å / Num. obs: 26543 / % possible obs: 99.8 % / Redundancy: 7.36 % / CC1/2: 0.99 / Rmerge(I) obs: 0.051 / Net I/σ(I): 22.5 |
Reflection shell | Resolution: 2.35→2.4 Å / Rmerge(I) obs: 0.615 / Mean I/σ(I) obs: 2.08 / Num. unique obs: 1932 / CC1/2: 0.972 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: PDB entry 3D5K Resolution: 2.35→30.48 Å / Cross valid method: FREE R-VALUE /
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Displacement parameters | Biso mean: 82.67 Å2 | ||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2.35→30.48 Å
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Refine LS restraints |
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