TRANSPORT PROTEIN / CHMP4A ALIX BRO1 amphipathic-helix / Apoptosis / Host-virus interaction / Protein transport / Transport / Cytoplasmic vesicle / Lipid-binding / Membrane
Function / homology
Function and homology information
proteinase activated receptor binding / membrane invagination / actomyosin contractile ring assembly / ubiquitin-independent protein catabolic process via the multivesicular body sorting pathway / regulation of extracellular exosome assembly / plasma membrane tubulation / amphisome membrane / multivesicular body-lysosome fusion / viral budding / extracellular exosome biogenesis ...proteinase activated receptor binding / membrane invagination / actomyosin contractile ring assembly / ubiquitin-independent protein catabolic process via the multivesicular body sorting pathway / regulation of extracellular exosome assembly / plasma membrane tubulation / amphisome membrane / multivesicular body-lysosome fusion / viral budding / extracellular exosome biogenesis / vesicle fusion with vacuole / maintenance of epithelial cell apical/basal polarity / positive regulation of extracellular exosome assembly / late endosome to lysosome transport / ESCRT III complex / kinetochore microtubule / regulation of membrane permeability / late endosome to vacuole transport via multivesicular body sorting pathway / regulation of centrosome duplication / nuclear membrane reassembly / Sealing of the nuclear envelope (NE) by ESCRT-III / midbody abscission / multivesicular body sorting pathway / bicellular tight junction assembly / vesicle budding from membrane / actomyosin / membrane coat / post-translational protein targeting to endoplasmic reticulum membrane / membrane fission / plasma membrane repair / multivesicular body membrane / positive regulation of exosomal secretion / ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway / multivesicular body assembly / regulation of mitotic spindle assembly / Translation of Replicase and Assembly of the Replication Transcription Complex / Flemming body / nervous system process / RIPK1-mediated regulated necrosis / mitotic metaphase chromosome alignment / Macroautophagy / nucleus organization / viral budding via host ESCRT complex / endoplasmic reticulum exit site / autophagosome membrane / Uptake and function of anthrax toxins / autophagosome maturation / immunological synapse / mitotic cytokinesis / protein polymerization / bicellular tight junction / Pyroptosis / nuclear pore / Endosomal Sorting Complex Required For Transport (ESCRT) / multivesicular body / viral budding from plasma membrane / HCMV Late Events / macroautophagy / Late endosomal microautophagy / Budding and maturation of HIV virion / protein homooligomerization / Regulation of necroptotic cell death / cytoplasmic side of plasma membrane / kinetochore / autophagy / calcium-dependent protein binding / extracellular vesicle / melanosome / protein transport / Translation of Replicase and Assembly of the Replication Transcription Complex / midbody / ATPase binding / endosome / lysosomal membrane / focal adhesion / centrosome / lipid binding / apoptotic process / protein homodimerization activity / extracellular exosome / identical protein binding / membrane / nucleus / plasma membrane / cytoplasm / cytosol Similarity search - Function
alix/aip1 like domains / Vacuolar protein-sorting protein Bro1-like / ALIX V-shaped domain / ALIX V-shaped domain binding to HIV / BRO1 domain / BRO1 domain superfamily / BRO1-like domain / BRO1 domain profile. / BRO1-like domain / Snf7 family ...alix/aip1 like domains / Vacuolar protein-sorting protein Bro1-like / ALIX V-shaped domain / ALIX V-shaped domain binding to HIV / BRO1 domain / BRO1 domain superfamily / BRO1-like domain / BRO1 domain profile. / BRO1-like domain / Snf7 family / Snf7 / Serine Threonine Protein Phosphatase 5, Tetratricopeptide repeat / Alpha Horseshoe / Mainly Alpha Similarity search - Domain/homology
Programmedcelldeath6-interactingprotein / PDCD6-interacting protein / ALG-2-interacting protein 1 / Hp95
Mass: 42735.789 Da / Num. of mol.: 1 / Fragment: BRO1 domain (UNP residues 1-358) Source method: isolated from a genetically manipulated source Source: (gene. exp.) Homo sapiens (human) / Gene: PDCD6IP, AIP1, ALIX, KIAA1375 / Production host: Escherichia coli (E. coli) / References: UniProt: Q8WUM4
#2: Protein/peptide
Chargedmultivesicularbodyprotein4apeptide / Chromatin-modifying protein 4a / CHMP4a / Vacuolar protein-sorting-associated protein 7-1 / SNF7- 1 ...Chromatin-modifying protein 4a / CHMP4a / Vacuolar protein-sorting-associated protein 7-1 / SNF7- 1 / hSnf-1 / SNF7 homolog associated with Alix-2
Mass: 1518.643 Da / Num. of mol.: 1 / Source method: obtained synthetically Details: CHMP4A peptide chemically synthesized. The sequence is found naturally in Homo sapiens (humans). References: UniProt: Q9BY43
Mass: 18.015 Da / Num. of mol.: 127 / Source method: isolated from a natural source / Formula: H2O
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Experimental details
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Experiment
Experiment
Method: X-RAY DIFFRACTION / Number of used crystals: 1
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Sample preparation
Crystal
Density Matthews: 2.75 Å3/Da / Density % sol: 55.34 %
Crystal grow
Temperature: 277 K / Method: vapor diffusion, sitting drop / pH: 6.5 Details: 15% PEG 8,000, 100mM Na MES pH 6.5, 200mM Na Acetate, VAPOR DIFFUSION, SITTING DROP, temperature 277K
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