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Yorodumi- PDB-6tri: CI-MOR repressor-antirepressor complex of the temperate bacteriop... -
+Open data
-Basic information
Entry | Database: PDB / ID: 6tri | ||||||
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Title | CI-MOR repressor-antirepressor complex of the temperate bacteriophage TP901-1 from Lactococcus lactis | ||||||
Components |
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Keywords | VIRAL PROTEIN / Complex / regulation / DNA-binding / temperate virus | ||||||
Function / homology | Function and homology information | ||||||
Biological species | Lactococcus phage TP901-1 (virus) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 2.277 Å | ||||||
Authors | Rasmussen, K.K. / Blackledge, M. / Herrmann, T. / Jensen, M.R. / Lo Leggio, L. | ||||||
Funding support | Denmark, 1items
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Citation | Journal: Proc.Natl.Acad.Sci.USA / Year: 2020 Title: Revealing the mechanism of repressor inactivation during switching of a temperate bacteriophage. Authors: Rasmussen, K.K. / Palencia, A. / Varming, A.K. / El-Wali, H. / Boeri Erba, E. / Blackledge, M. / Hammer, K. / Herrmann, T. / Kilstrup, M. / Lo Leggio, L. / Jensen, M.R. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 6tri.cif.gz | 75.8 KB | Display | PDBx/mmCIF format |
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PDB format | pdb6tri.ent.gz | 55.6 KB | Display | PDB format |
PDBx/mmJSON format | 6tri.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 6tri_validation.pdf.gz | 422.1 KB | Display | wwPDB validaton report |
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Full document | 6tri_full_validation.pdf.gz | 422.1 KB | Display | |
Data in XML | 6tri_validation.xml.gz | 7.6 KB | Display | |
Data in CIF | 6tri_validation.cif.gz | 9.1 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/tr/6tri ftp://data.pdbj.org/pub/pdb/validation_reports/tr/6tri | HTTPS FTP |
-Related structure data
Related structure data | 6to6SC 5a7lS S: Starting model for refinement C: citing same article (ref.) |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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Components on special symmetry positions |
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-Components
#1: Protein | Mass: 10947.503 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Lactococcus phage TP901-1 (virus) / Gene: cI / Production host: Escherichia coli (E. coli) / References: UniProt: O48503 | ||||
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#2: Protein | Mass: 8422.644 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Lactococcus phage TP901-1 (virus) / Gene: mor / Production host: Escherichia coli (E. coli) / References: UniProt: O48504 | ||||
#3: Chemical | #4: Water | ChemComp-HOH / | Has ligand of interest | N | |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 2.03 Å3/Da / Density % sol: 39.39 % |
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Crystal grow | Temperature: 293 K / Method: vapor diffusion, sitting drop Details: 0.2 M lithium sulfate, 0.1 M Bis-Tris pH 5.5, 25% PEG 3350 in reservoir |
-Data collection
Diffraction | Mean temperature: 100 K / Serial crystal experiment: N |
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Diffraction source | Source: SYNCHROTRON / Site: ESRF / Beamline: ID23-1 / Wavelength: 0.972 Å |
Detector | Type: DECTRIS PILATUS3 S 6M / Detector: PIXEL / Date: Feb 4, 2018 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.972 Å / Relative weight: 1 |
Reflection | Resolution: 2.277→42.7 Å / Num. obs: 7285 / % possible obs: 99.9 % / Redundancy: 16.1 % / CC1/2: 0.996 / Net I/σ(I): 7.95 |
Reflection shell | Resolution: 2.277→2.41 Å / Num. unique obs: 1184 / CC1/2: 0.377 |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: 5A7L + MOR NMR structure (just deposited 6TO6) Resolution: 2.277→40.879 Å / SU ML: 0.29 / Cross valid method: THROUGHOUT / σ(F): 1.34 / Phase error: 28.3
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso max: 180.6 Å2 / Biso mean: 58.2949 Å2 / Biso min: 29.95 Å2 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: final / Resolution: 2.277→40.879 Å
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Refine LS restraints |
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LS refinement shell |
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Refinement TLS params. | Method: refined / Refine-ID: X-RAY DIFFRACTION
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Refinement TLS group |
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