|Entry||Database: PDB / ID: 6tc2|
|Title||Monoclinic human insulin in complex with p-coumaric acid|
|Keywords||HORMONE / HUMAN INSULIN / p-coumaric acid / HEXAMER / COMPLEX|
|Function / homology|
Function and homology information
Signaling by Insulin receptor / negative regulation of glycogen catabolic process / alpha-beta T cell activation / regulation of cellular amino acid metabolic process / IRS activation / Insulin processing / negative regulation of NAD(P)H oxidase activity / Insulin receptor recycling / nitric oxide-cGMP-mediated signaling pathway / negative regulation of fatty acid metabolic process ...Signaling by Insulin receptor / negative regulation of glycogen catabolic process / alpha-beta T cell activation / regulation of cellular amino acid metabolic process / IRS activation / Insulin processing / negative regulation of NAD(P)H oxidase activity / Insulin receptor recycling / nitric oxide-cGMP-mediated signaling pathway / negative regulation of fatty acid metabolic process / negative regulation of feeding behavior / regulation of protein secretion / Regulation of gene expression in beta cells / positive regulation of respiratory burst / positive regulation of peptide hormone secretion / negative regulation of respiratory burst involved in inflammatory response / negative regulation of gluconeogenesis / positive regulation of protein metabolic process => GO:0051247 / negative regulation of acute inflammatory response / positive regulation of dendritic spine maintenance / COPI-mediated anterograde transport / Synthesis, secretion, and deacylation of Ghrelin / positive regulation of glycogen biosynthetic process / regulation of protein localization to plasma membrane / negative regulation of reactive oxygen species biosynthetic process / negative regulation of oxidative stress-induced intrinsic apoptotic signaling pathway / Signal attenuation / negative regulation of protein secretion / FOXO-mediated transcription of oxidative stress, metabolic and neuronal genes / positive regulation of nitric oxide mediated signal transduction / positive regulation of lipid biosynthetic process / negative regulation of lipid catabolic process / fatty acid homeostasis / endosome lumen / Regulation of insulin secretion / positive regulation of insulin receptor signaling pathway / endoplasmic reticulum-Golgi intermediate compartment membrane / neuron projection maintenance / transport vesicle / insulin-like growth factor receptor binding / positive regulation of protein autophosphorylation / positive regulation of glycolytic process / Insulin receptor signalling cascade / positive regulation of brown fat cell differentiation / positive regulation of mitotic nuclear division / positive regulation of cell differentiation / regulation of transmembrane transporter activity / positive regulation of long-term synaptic potentiation / regulation of synaptic plasticity / cognition / activation of protein kinase B activity / positive regulation of cytokine production / positive regulation of protein secretion / positive regulation of glucose import / positive regulation of nitric-oxide synthase activity / acute-phase response / negative regulation of proteolysis / hormone activity / vasodilation / insulin receptor binding / insulin receptor signaling pathway / negative regulation of protein catabolic process / positive regulation of protein localization to nucleus / wound healing / Golgi lumen / glucose metabolic process / regulation of protein localization / cell-cell signaling / glucose homeostasis / positive regulation of phosphatidylinositol 3-kinase signaling / PI5P, PP2A and IER3 Regulate PI3K/AKT Signaling / positive regulation of cell growth / secretory granule lumen / positive regulation of NF-kappaB transcription factor activity / positive regulation of protein kinase B signaling / protease binding / positive regulation of MAPK cascade / G protein-coupled receptor signaling pathway / positive regulation of cell migration / Amyloid fiber formation / Golgi membrane / negative regulation of gene expression / endoplasmic reticulum lumen / positive regulation of cell population proliferation / positive regulation of gene expression / regulation of transcription, DNA-templated / extracellular space / extracellular region / identical protein binding
Similarity search - Function
Insulin / Insulin family / Insulin / insulin-like growth factor / relaxin family. / Insulin/IGF/Relaxin family / Insulin-like / Insulin family signature. / Insulin-like superfamily / Insulin, conserved site
Similarity search - Domain/homology
4'-HYDROXYCINNAMIC ACID / PHOSPHATE ION / THIOCYANATE ION / Insulin
Similarity search - Component
|Biological species||Homo sapiens (human)|
|Method||X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / molecular replacement / Resolution: 1.36 Å|
|Authors||Triandafillidis, D.-P. / Parthenios, N. / Spiliopoulou, M. / Valmas, A. / Kosinas, C. / Gozzo, F. / Reinle-Schmitt, M. / Beckers, D. / Degen, T. / Pop, M. ...Triandafillidis, D.-P. / Parthenios, N. / Spiliopoulou, M. / Valmas, A. / Kosinas, C. / Gozzo, F. / Reinle-Schmitt, M. / Beckers, D. / Degen, T. / Pop, M. / Fitch, A. / Wollenhaupt, J. / Weiss, M.S. / Karavassili, F. / Margiolaki, I.|
|Funding support|| Greece, 2items |
|Citation||Journal: Acta Crystallogr D Struct Biol / Year: 2020|
Title: Insulin polymorphism induced by two polyphenols: new crystal forms and advances in macromolecular powder diffraction.
Authors: Triandafillidis, D.P. / Parthenios, N. / Spiliopoulou, M. / Valmas, A. / Kosinas, C. / Gozzo, F. / Reinle-Schmitt, M. / Beckers, D. / Degen, T. / Pop, M. / Fitch, A.N. / Wollenhaupt, J. / ...Authors: Triandafillidis, D.P. / Parthenios, N. / Spiliopoulou, M. / Valmas, A. / Kosinas, C. / Gozzo, F. / Reinle-Schmitt, M. / Beckers, D. / Degen, T. / Pop, M. / Fitch, A.N. / Wollenhaupt, J. / Weiss, M.S. / Karavassili, F. / Margiolaki, I.
|Structure viewer||Molecule: |
Downloads & links
-Protein , 1 types, 12 molecules A
B C D E F G H I J K L
Mass: 11989.862 Da / Num. of mol.: 12
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human) / Gene: INS / Production host: Saccharomyces cerevisiae (baker's yeast) / References: UniProt: P01308
-Non-polymers , 5 types, 200 molecules
|#2: Chemical||#3: Chemical||#4: Chemical|
Mass: 164.158 Da / Num. of mol.: 5 / Source method: obtained synthetically / Formula: C9H8O3 / Feature type: SUBJECT OF INVESTIGATION
|#5: Chemical|| ChemComp-SCN / ||#6: Water|| ChemComp-HOH / |
|Has ligand of interest||Y|
|Experiment||Method: X-RAY DIFFRACTION / Number of used crystals: 1|
|Crystal||Density Matthews: 1.95 Å3/Da / Density % sol: 36.94 %|
|Crystal grow||Temperature: 298 K / Method: batch mode / pH: 5.82 |
Details: 12.78 mg/mL insulin, 0.77 mM zinc acetate, 40 mM p-coumaric acid, 3.2%(w/v) PEG-1000, 10 mM sodium thiocyanate, 0.4 M phosphate mixture (Na2HPO4, KH2PO4), pH = 5.82
|Diffraction||Mean temperature: 100 K / Serial crystal experiment: N|
|Diffraction source||Source: SYNCHROTRON / Site: BESSY / Beamline: 14.1 / Wavelength: 0.9184 Å|
|Detector||Type: DECTRIS PILATUS 6M / Detector: PIXEL / Date: Jun 16, 2019|
|Radiation||Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray|
|Radiation wavelength||Wavelength: 0.9184 Å / Relative weight: 1|
|Reflection||Resolution: 1.36→47.423 Å / Num. obs: 54569 / % possible obs: 98.2 % / Redundancy: 12.727 % / Biso Wilson estimate: 26.592 Å2 / CC1/2: 1 / Rrim(I) all: 0.063 / Χ2: 1.061 / Net I/σ(I): 18.08|
|Phasing||Method: molecular replacement|
|Refinement||Method to determine structure: MOLECULAR REPLACEMENT|
Starting model: 1EV6
Resolution: 1.36→47.423 Å / Cross valid method: THROUGHOUT
|Displacement parameters||Biso max: 133.54 Å2 / Biso mean: 34.3236 Å2 / Biso min: 10.48 Å2|
|Refinement step||Cycle: LAST / Resolution: 1.36→47.423 Å|
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