+データを開く
-基本情報
登録情報 | データベース: PDB / ID: 6tc2 | |||||||||
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タイトル | Monoclinic human insulin in complex with p-coumaric acid | |||||||||
要素 | Insulinインスリン | |||||||||
キーワード | HORMONE (ホルモン) / HUMAN INSULIN (インスリン製剤) / p-coumaric acid (P-クマル酸) / HEXAMER (オリゴマー) / COMPLEX | |||||||||
機能・相同性 | 機能・相同性情報 negative regulation of NAD(P)H oxidase activity / negative regulation of glycogen catabolic process / regulation of cellular amino acid metabolic process / Signaling by Insulin receptor / IRS activation / nitric oxide-cGMP-mediated signaling / negative regulation of fatty acid metabolic process / Insulin processing / negative regulation of feeding behavior / regulation of protein secretion ...negative regulation of NAD(P)H oxidase activity / negative regulation of glycogen catabolic process / regulation of cellular amino acid metabolic process / Signaling by Insulin receptor / IRS activation / nitric oxide-cGMP-mediated signaling / negative regulation of fatty acid metabolic process / Insulin processing / negative regulation of feeding behavior / regulation of protein secretion / positive regulation of peptide hormone secretion / Regulation of gene expression in beta cells / positive regulation of respiratory burst / positive regulation of dendritic spine maintenance / alpha-beta T cell activation / negative regulation of acute inflammatory response / negative regulation of respiratory burst involved in inflammatory response / negative regulation of protein secretion / fatty acid homeostasis / Synthesis, secretion, and deacylation of Ghrelin / positive regulation of glycogen biosynthetic process / positive regulation of lipid biosynthetic process / Signal attenuation / FOXO-mediated transcription of oxidative stress, metabolic and neuronal genes / negative regulation of gluconeogenesis / positive regulation of nitric oxide mediated signal transduction / regulation of protein localization to plasma membrane / COPI-mediated anterograde transport / negative regulation of lipid catabolic process / negative regulation of oxidative stress-induced intrinsic apoptotic signaling pathway / positive regulation of insulin receptor signaling pathway / negative regulation of reactive oxygen species biosynthetic process / 小胞 / positive regulation of protein autophosphorylation / Insulin receptor recycling / insulin-like growth factor receptor binding / NPAS4 regulates expression of target genes / positive regulation of protein metabolic process / neuron projection maintenance / endoplasmic reticulum-Golgi intermediate compartment membrane / positive regulation of brown fat cell differentiation / activation of protein kinase B activity / positive regulation of glycolytic process / Insulin receptor signalling cascade / positive regulation of mitotic nuclear division / Regulation of insulin secretion / positive regulation of nitric-oxide synthase activity / positive regulation of long-term synaptic potentiation / endosome lumen / positive regulation of cytokine production / acute-phase response / positive regulation of protein secretion / regulation of transmembrane transporter activity / positive regulation of cell differentiation / positive regulation of glucose import / negative regulation of proteolysis / regulation of synaptic plasticity / wound healing / insulin receptor binding / negative regulation of protein catabolic process / positive regulation of neuron projection development / hormone activity / 認識 / Golgi lumen / vasodilation / positive regulation of protein localization to nucleus / glucose metabolic process / regulation of protein localization / glucose homeostasis / cell-cell signaling / insulin receptor signaling pathway / positive regulation of NF-kappaB transcription factor activity / PI5P, PP2A and IER3 Regulate PI3K/AKT Signaling / positive regulation of cell growth / secretory granule lumen / protease binding / positive regulation of MAPK cascade / positive regulation of phosphatidylinositol 3-kinase/protein kinase B signal transduction / positive regulation of cell migration / G protein-coupled receptor signaling pathway / Amyloid fiber formation / 小胞体 / ゴルジ体 / negative regulation of gene expression / positive regulation of cell population proliferation / positive regulation of gene expression / regulation of DNA-templated transcription / extracellular space / extracellular region / identical protein binding 類似検索 - 分子機能 | |||||||||
生物種 | Homo sapiens (ヒト) | |||||||||
手法 | X線回折 / シンクロトロン / 分子置換 / 解像度: 1.36 Å | |||||||||
データ登録者 | Triandafillidis, D.-P. / Parthenios, N. / Spiliopoulou, M. / Valmas, A. / Kosinas, C. / Gozzo, F. / Reinle-Schmitt, M. / Beckers, D. / Degen, T. / Pop, M. ...Triandafillidis, D.-P. / Parthenios, N. / Spiliopoulou, M. / Valmas, A. / Kosinas, C. / Gozzo, F. / Reinle-Schmitt, M. / Beckers, D. / Degen, T. / Pop, M. / Fitch, A. / Wollenhaupt, J. / Weiss, M.S. / Karavassili, F. / Margiolaki, I. | |||||||||
資金援助 | ギリシャ, 2件
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引用 | ジャーナル: Acta Crystallogr D Struct Biol / 年: 2020 タイトル: Insulin polymorphism induced by two polyphenols: new crystal forms and advances in macromolecular powder diffraction. 著者: Triandafillidis, D.P. / Parthenios, N. / Spiliopoulou, M. / Valmas, A. / Kosinas, C. / Gozzo, F. / Reinle-Schmitt, M. / Beckers, D. / Degen, T. / Pop, M. / Fitch, A.N. / Wollenhaupt, J. / ...著者: Triandafillidis, D.P. / Parthenios, N. / Spiliopoulou, M. / Valmas, A. / Kosinas, C. / Gozzo, F. / Reinle-Schmitt, M. / Beckers, D. / Degen, T. / Pop, M. / Fitch, A.N. / Wollenhaupt, J. / Weiss, M.S. / Karavassili, F. / Margiolaki, I. | |||||||||
履歴 |
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-構造の表示
構造ビューア | 分子: MolmilJmol/JSmol |
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-ダウンロードとリンク
-ダウンロード
PDBx/mmCIF形式 | 6tc2.cif.gz | 207.5 KB | 表示 | PDBx/mmCIF形式 |
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PDB形式 | pdb6tc2.ent.gz | 161.2 KB | 表示 | PDB形式 |
PDBx/mmJSON形式 | 6tc2.json.gz | ツリー表示 | PDBx/mmJSON形式 | |
その他 | その他のダウンロード |
-検証レポート
アーカイブディレクトリ | https://data.pdbj.org/pub/pdb/validation_reports/tc/6tc2 ftp://data.pdbj.org/pub/pdb/validation_reports/tc/6tc2 | HTTPS FTP |
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-関連構造データ
関連構造データ | 1ev6S S: 精密化の開始モデル |
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類似構造データ |
-リンク
-集合体
登録構造単位 |
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1 |
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単位格子 |
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-要素
-タンパク質 , 1種, 12分子 ABCDEFGHIJKL
#1: タンパク質 | 分子量: 11989.862 Da / 分子数: 12 / 由来タイプ: 組換発現 / 由来: (組換発現) Homo sapiens (ヒト) / 遺伝子: INS / 発現宿主: Saccharomyces cerevisiae (パン酵母) / 参照: UniProt: P01308 |
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-非ポリマー , 5種, 200分子
#2: 化合物 | #3: 化合物 | #4: 化合物 | ChemComp-HC4 / #5: 化合物 | ChemComp-SCN / | #6: 水 | ChemComp-HOH / | |
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-詳細
研究の焦点であるリガンドがあるか | Y |
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-実験情報
-実験
実験 | 手法: X線回折 / 使用した結晶の数: 1 |
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-試料調製
結晶 | マシュー密度: 1.95 Å3/Da / 溶媒含有率: 36.94 % |
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結晶化 | 温度: 298 K / 手法: batch mode / pH: 5.82 詳細: 12.78 mg/mL insulin, 0.77 mM zinc acetate, 40 mM p-coumaric acid, 3.2%(w/v) PEG-1000, 10 mM sodium thiocyanate, 0.4 M phosphate mixture (Na2HPO4, KH2PO4), pH = 5.82 |
-データ収集
回折 | 平均測定温度: 100 K / Serial crystal experiment: N | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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放射光源 | 由来: シンクロトロン / サイト: BESSY / ビームライン: 14.1 / 波長: 0.9184 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
検出器 | タイプ: DECTRIS PILATUS 6M / 検出器: PIXEL / 日付: 2019年6月16日 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
放射 | プロトコル: SINGLE WAVELENGTH / 単色(M)・ラウエ(L): M / 散乱光タイプ: x-ray | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
放射波長 | 波長: 0.9184 Å / 相対比: 1 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
反射 | 解像度: 1.36→47.423 Å / Num. obs: 54569 / % possible obs: 98.2 % / 冗長度: 12.727 % / Biso Wilson estimate: 26.592 Å2 / CC1/2: 1 / Rrim(I) all: 0.063 / Χ2: 1.061 / Net I/σ(I): 18.08 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
反射 シェル | Diffraction-ID: 1
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-位相決定
位相決定 | 手法: 分子置換 |
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-解析
ソフトウェア |
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精密化 | 構造決定の手法: 分子置換 開始モデル: 1EV6 解像度: 1.36→47.423 Å / 交差検証法: THROUGHOUT
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原子変位パラメータ | Biso max: 133.54 Å2 / Biso mean: 34.3236 Å2 / Biso min: 10.48 Å2 | ||||||||||||||||||
精密化ステップ | サイクル: LAST / 解像度: 1.36→47.423 Å
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