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- PDB-6sr6: Crystal structure of the RAC core with a pseudo substrate bound t... -
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Open data
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Basic information
Entry | Database: PDB / ID: 6sr6 | |||||||||
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Title | Crystal structure of the RAC core with a pseudo substrate bound to Ssz1 SBD | |||||||||
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![]() | CHAPERONE / Hsp70 | |||||||||
Function / homology | ![]() 'de novo' cotranslational protein folding / regulation of translational fidelity / Hsp70 protein binding / ATP-dependent protein folding chaperone / ribosome binding / ATP binding / nucleus / cytosol Similarity search - Function | |||||||||
Biological species | ![]() | |||||||||
Method | ![]() ![]() ![]() | |||||||||
![]() | Valentin Gese, G. / Lapouge, K. / Kopp, J. / Sinning, I. | |||||||||
Funding support | ![]()
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![]() | ![]() Title: The ribosome-associated complex RAC serves in a relay that directs nascent chains to Ssb. Authors: Zhang, Y. / Valentin Gese, G. / Conz, C. / Lapouge, K. / Kopp, J. / Wolfle, T. / Rospert, S. / Sinning, I. | |||||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 453.6 KB | Display | ![]() |
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PDB format | ![]() | 368.7 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 1 MB | Display | ![]() |
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Full document | ![]() | 1.1 MB | Display | |
Data in XML | ![]() | 39.4 KB | Display | |
Data in CIF | ![]() | 54.3 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 5mb9S S: Starting model for refinement |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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1 | ![]()
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2 | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 63706.820 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() Strain: DSM 1495 / CBS 144.50 / IMI 039719 / Gene: CTHT_0008010 / Production host: ![]() ![]() #2: Protein | Mass: 6906.990 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() Strain: DSM 1495 / CBS 144.50 / IMI 039719 / Gene: CTHT_0006310 / Production host: ![]() ![]() #3: Chemical | #4: Chemical | #5: Water | ChemComp-HOH / | Has ligand of interest | N | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.83 Å3/Da / Density % sol: 56.48 % |
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Crystal grow | Temperature: 291 K / Method: vapor diffusion, sitting drop / pH: 7.5 / Details: 20.5% (v/v) PEG 3350, 0.2 M ammonium acetate |
-Data collection
Diffraction | Mean temperature: 100 K / Serial crystal experiment: N |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: DECTRIS EIGER X 4M / Detector: PIXEL / Date: May 5, 2018 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.9677 Å / Relative weight: 1 |
Reflection | Resolution: 2.5→48.4 Å / Num. obs: 46860 / % possible obs: 98.9 % / Redundancy: 6 % / Biso Wilson estimate: 38.97 Å2 / Rmerge(I) obs: 0.134 / Net I/σ(I): 9.6 |
Reflection shell | Resolution: 2.5→2.59 Å / Rmerge(I) obs: 0.706 / Mean I/σ(I) obs: 2.2 / Num. unique obs: 4516 / CC1/2: 0.658 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: 5MB9 Resolution: 2.5→33.71 Å / Cross valid method: FREE R-VALUE
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Refinement step | Cycle: LAST / Resolution: 2.5→33.71 Å
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