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- PDB-6se3: Crystal Structure of Ancestral Flavin-containing monooxygenase (F... -
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Open data
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Basic information
Entry | Database: PDB / ID: 6se3 | ||||||
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Title | Crystal Structure of Ancestral Flavin-containing monooxygenase (FMO) 3-6 | ||||||
![]() | Ancestral Flavin-containing monooxygenase (FMO) 3-6 | ||||||
![]() | MEMBRANE PROTEIN / Flavin / enzyme / Ancestral Sequence Reconstruction | ||||||
Function / homology | FLAVIN-ADENINE DINUCLEOTIDE / NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE / OXYGEN MOLECULE![]() | ||||||
Biological species | synthetic construct (others) | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Nicoll, C. / Bailleul, G. / Fiorentini, F. / Mascotti, M.L. / Fraaije, M. / Mattevi, A. | ||||||
Funding support | 1items
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![]() | ![]() Title: Ancestral-sequence reconstruction unveils the structural basis of function in mammalian FMOs. Authors: Nicoll, C.R. / Bailleul, G. / Fiorentini, F. / Mascotti, M.L. / Fraaije, M.W. / Mattevi, A. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 643 KB | Display | ![]() |
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PDB format | ![]() | 531.8 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
Related structure data | ![]() 6sekC ![]() 6semC ![]() 6sf0C ![]() 5nmwS S: Starting model for refinement C: citing same article ( |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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1 | ![]()
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2 | ![]()
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3 | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 60127.992 Da / Num. of mol.: 6 Source method: isolated from a genetically manipulated source Details: Residues at the N and C termini were removed from the structural coordinates due to a lack of clear electron density Source: (gene. exp.) synthetic construct (others) / Production host: ![]() ![]() #2: Chemical | ChemComp-FAD / #3: Chemical | ChemComp-NAP / #4: Chemical | ChemComp-OXY / #5: Water | ChemComp-HOH / | Has ligand of interest | N | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 3.64 Å3/Da / Density % sol: 66.23 % |
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Crystal grow | Temperature: 293 K / Method: vapor diffusion, sitting drop / pH: 5.5 / Details: PEG 4000, Sodium Acetate |
-Data collection
Diffraction | Mean temperature: 100 K / Serial crystal experiment: N |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: DECTRIS PILATUS 2M-F / Detector: PIXEL / Date: Dec 15, 2018 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.98 Å / Relative weight: 1 |
Reflection | Resolution: 2.8→49.3 Å / Num. obs: 129389 / % possible obs: 100 % / Redundancy: 20.3 % / CC1/2: 0.998 / Rmerge(I) obs: 0.235 / Net I/σ(I): 14.3 |
Reflection shell | Resolution: 2.8→2.85 Å / Rmerge(I) obs: 2.876 / Mean I/σ(I) obs: 1.3 / Num. unique obs: 6372 / CC1/2: 0.409 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: 5nmw Resolution: 2.8→49.3 Å / Cross valid method: THROUGHOUT
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Displacement parameters | Biso mean: 72 Å2 | ||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2.8→49.3 Å
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