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Structure paper

TitleAncestral-sequence reconstruction unveils the structural basis of function in mammalian FMOs.
Journal, issue, pagesNat. Struct. Mol. Biol., Vol. 27, Page 14-24, Year 2020
Publish dateJul 29, 2019 (structure data deposition date)
AuthorsNicoll, C.R. / Bailleul, G. / Fiorentini, F. / Mascotti, M.L. / Fraaije, M.W. / Mattevi, A.
External linksNat. Struct. Mol. Biol. / PubMed:31873300
MethodsX-ray diffraction
Resolution2.7 - 3.01 Å
Structure data

PDB-6se3:
Crystal Structure of Ancestral Flavin-containing monooxygenase (FMO) 3-6
Method: X-RAY DIFFRACTION / Resolution: 2.8 Å

PDB-6sek:
Crystal Structure of Ancestral Flavin-containing monooxygenase (FMO) 5
Method: X-RAY DIFFRACTION / Resolution: 2.7 Å

PDB-6sem:
Crystal Structure of Ancestral Flavin-containing monooxygenase (FMO) 2
Method: X-RAY DIFFRACTION / Resolution: 2.8 Å

PDB-6sf0:
Crystal Structure of Ancestral Flavin-containing monooxygenase (FMO) 2 in the presence of NADP+
Method: X-RAY DIFFRACTION / Resolution: 3.01 Å

Chemicals

ChemComp-FAD:
FLAVIN-ADENINE DINUCLEOTIDE / FAD*YM

ChemComp-NAP:
NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE

ChemComp-OXY:
OXYGEN MOLECULE

ChemComp-HOH:
WATER

ChemComp-LMT:
DODECYL-BETA-D-MALTOSIDE / detergent*YM

ChemComp-EPE:
4-(2-HYDROXYETHYL)-1-PIPERAZINE ETHANESULFONIC ACID / pH buffer*YM

ChemComp-GLC:
alpha-D-glucopyranose

ChemComp-MA4:
CYCLOHEXYL-HEXYL-BETA-D-MALTOSIDE

Source
  • synthetic construct (others)
KeywordsMEMBRANE PROTEIN / Flavin / enzyme / Ancestral Sequence Reconstruction / OXIDOREDUCTASE

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