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- PDB-6rz8: Crystal structure of the human cysteinyl leukotriene receptor 2 i... -
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Open data
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Basic information
Entry | Database: PDB / ID: 6rz8 | |||||||||||||||
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Title | Crystal structure of the human cysteinyl leukotriene receptor 2 in complex with ONO-2080365 | |||||||||||||||
![]() | Cysteinyl leukotriene receptor 2,Soluble cytochrome b562,Cysteinyl leukotriene receptor 2 | |||||||||||||||
![]() | MEMBRANE PROTEIN / GPCR / LCP / cysteinyl leukotriene / cyslt2 / cysltr2 / cyslt2r / asthma / BRIL / Cysteinyl Leukotriene Receptor 2 | |||||||||||||||
Function / homology | ![]() cysteinyl leukotriene receptor activity / leukotriene receptor activity / Leukotriene receptors / LTC4-CYSLTR mediated IL4 production / G protein-coupled peptide receptor activity / neuropeptide signaling pathway / electron transport chain / G alpha (s) signalling events / G alpha (q) signalling events / periplasmic space ...cysteinyl leukotriene receptor activity / leukotriene receptor activity / Leukotriene receptors / LTC4-CYSLTR mediated IL4 production / G protein-coupled peptide receptor activity / neuropeptide signaling pathway / electron transport chain / G alpha (s) signalling events / G alpha (q) signalling events / periplasmic space / electron transfer activity / iron ion binding / immune response / heme binding / plasma membrane Similarity search - Function | |||||||||||||||
Biological species | ![]() ![]() ![]() | |||||||||||||||
Method | ![]() ![]() ![]() | |||||||||||||||
![]() | Gusach, A. / Luginina, A. / Marin, E. / Brouillette, R.L. / Besserer-Offroy, E. / Longpre, J.M. / Ishchenko, A. / Popov, P. / Fujimoto, T. / Maruyama, T. ...Gusach, A. / Luginina, A. / Marin, E. / Brouillette, R.L. / Besserer-Offroy, E. / Longpre, J.M. / Ishchenko, A. / Popov, P. / Fujimoto, T. / Maruyama, T. / Stauch, B. / Ergasheva, M. / Romanovskaya, D. / Stepko, A. / Kovalev, K. / Shevtsov, M. / Gordeliy, V. / Han, G.W. / Sarret, P. / Katritch, V. / Borshchevskiy, V. / Mishin, A. / Cherezov, V. | |||||||||||||||
Funding support | ![]() ![]() ![]()
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![]() | ![]() Title: Structural basis of ligand selectivity and disease mutations in cysteinyl leukotriene receptors. Authors: Gusach, A. / Luginina, A. / Marin, E. / Brouillette, R.L. / Besserer-Offroy, E. / Longpre, J.M. / Ishchenko, A. / Popov, P. / Patel, N. / Fujimoto, T. / Maruyama, T. / Stauch, B. / ...Authors: Gusach, A. / Luginina, A. / Marin, E. / Brouillette, R.L. / Besserer-Offroy, E. / Longpre, J.M. / Ishchenko, A. / Popov, P. / Patel, N. / Fujimoto, T. / Maruyama, T. / Stauch, B. / Ergasheva, M. / Romanovskaia, D. / Stepko, A. / Kovalev, K. / Shevtsov, M. / Gordeliy, V. / Han, G.W. / Katritch, V. / Borshchevskiy, V. / Sarret, P. / Mishin, A. / Cherezov, V. | |||||||||||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 168.1 KB | Display | ![]() |
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PDB format | ![]() | 129.9 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
Related structure data | ![]() 6rz6C ![]() 6rz7C ![]() 6rz9C ![]() 6rz4S S: Starting model for refinement C: citing same article ( |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 45995.961 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() Gene: CYSLTR2, CYSLT2, CYSLT2R, PSEC0146, cybC / Cell line (production host): Sf9 / Production host: ![]() ![]() | ||||
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#2: Chemical | ChemComp-KNZ / ( | ||||
#3: Chemical | ChemComp-OLC / ( #4: Chemical | #5: Water | ChemComp-HOH / | |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.9 Å3/Da / Density % sol: 58 % |
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Crystal grow | Temperature: 293 K / Method: lipidic cubic phase / pH: 8 Details: 100 mM K Formate 30% v/v PEG400 100 mM TRIS-HCl pH 8.0 |
-Data collection
Diffraction | Mean temperature: 100 K / Serial crystal experiment: N |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: DECTRIS PILATUS3 6M / Detector: PIXEL / Date: Jun 23, 2017 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.97625 Å / Relative weight: 1 |
Reflection | Resolution: 2.7→30 Å / Num. obs: 14227 / % possible obs: 100 % / Redundancy: 14 % / CC1/2: 0.99 / Rmerge(I) obs: 0.239 / Rpim(I) all: 0.066 / Net I/σ(I): 9.8 |
Reflection shell | Resolution: 2.7→2.8 Å / Redundancy: 13.8 % / Rmerge(I) obs: 2.758 / Mean I/σ(I) obs: 1.2 / CC1/2: 0.41 / Rpim(I) all: 0.766 / % possible all: 100 |
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Processing
Software |
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Refinement | Method to determine structure: ![]() Starting model: 6RZ4 Resolution: 2.7→29.924 Å / SU ML: 0.39 / Cross valid method: FREE R-VALUE / σ(F): 1.36 / Phase error: 28.04
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 82.2 Å2 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2.7→29.924 Å
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Refine LS restraints |
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LS refinement shell |
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Refinement TLS params. | Method: refined / Refine-ID: X-RAY DIFFRACTION
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Refinement TLS group |
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