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- PDB-6rha: Crystal structure of the amyloid-like NTVTFN segment from the Can... -
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Open data
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Basic information
Entry | Database: PDB / ID: 6rha | ||||||
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Title | Crystal structure of the amyloid-like NTVTFN segment from the Candida albicans Agglutinin-like protein (Adhesin) 5 | ||||||
![]() | Agglutinin-like protein 5 | ||||||
![]() | PROTEIN FIBRIL / Bacterial steric-zipper cross-beta amyloid fibril from Candida albicans | ||||||
Function / homology | ![]() cell adhesion involved in multi-species biofilm formation / fungal-type cell wall / cell adhesion involved in single-species biofilm formation / side of membrane / cell adhesion molecule binding / cell-cell adhesion / extracellular region / plasma membrane Similarity search - Function | ||||||
Biological species | ![]() | ||||||
Method | ![]() ![]() ![]() ![]() | ||||||
Model details | Adhesin | ||||||
![]() | Landau, M. / Perov, S. | ||||||
![]() | ![]() Title: Amyloid structures from a Candida albicans adhesin Structure and conservation of amyloid spines from fungal adhesins Authors: Perov, S. / Landau, M. / Lipke, P.N. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 11.4 KB | Display | ![]() |
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PDB format | ![]() | 6 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 374.8 KB | Display | ![]() |
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Full document | ![]() | 374.8 KB | Display | |
Data in XML | ![]() | 2.6 KB | Display | |
Data in CIF | ![]() | 2.7 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
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Links
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Assembly
Deposited unit | ![]()
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1 | ![]()
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Unit cell |
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Components
#1: Protein/peptide | Mass: 694.733 Da / Num. of mol.: 2 Fragment: Amyloid spine segment NTVTFN from Als5 (residues 156-161) secreted by Candida albicans Source method: obtained synthetically / Details: NTVTFN from Als5, synthesized / Source: (synth.) ![]() #2: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal grow | Temperature: 293 K / Method: vapor diffusion, hanging drop / pH: 7.5 Details: Reservoir contained 0.1M HEPES 7.5 pH, 0.8 M NaH2PO4, and 0.8M KH2PO4 |
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-Data collection
Diffraction | Mean temperature: 100 K / Serial crystal experiment: N | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Diffraction source | Source: ![]() ![]() ![]() | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Detector | Type: DECTRIS PILATUS3 2M / Detector: PIXEL / Date: May 10, 2015 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Radiation wavelength | Wavelength: 0.8729 Å / Relative weight: 1 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Reflection | Resolution: 1.6→19.91 Å / Num. obs: 991 / % possible obs: 98.2 % / Redundancy: 14.28 % / Biso Wilson estimate: 19.469 Å2 / CC1/2: 0.975 / Rmerge(I) obs: 0.27 / Rrim(I) all: 0.28 / Χ2: 0.747 / Net I/σ(I): 7.3 / Num. measured all: 14151 / Scaling rejects: 28 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Reflection shell | Diffraction-ID: 1
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-Phasing
Phasing | Method: ![]() | |||||||||
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Phasing MR | Model details: Phaser MODE: MR_AUTO / Packing: 0
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Processing
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Refinement | Method to determine structure: ![]() Starting model: beta strand Resolution: 1.6→19.91 Å / Cor.coef. Fo:Fc: 0.939 / Cor.coef. Fo:Fc free: 0.928 / SU B: 2.893 / SU ML: 0.093 / SU R Cruickshank DPI: 0.1536 / Cross valid method: THROUGHOUT / σ(F): 0 / ESU R: 0.154 / ESU R Free: 0.121 Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS U VALUES : REFINED INDIVIDUALLY
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Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å | |||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso max: 31.18 Å2 / Biso mean: 10.562 Å2 / Biso min: 6.63 Å2
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Refinement step | Cycle: final / Resolution: 1.6→19.91 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 1.601→1.643 Å / Rfactor Rfree error: 0 / Total num. of bins used: 20
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