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- PDB-6qil: The complex structure of hsRosR-S1 (VNG0258H/RosR-S1) -

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Basic information

Entry
Database: PDB / ID: 6qil
TitleThe complex structure of hsRosR-S1 (VNG0258H/RosR-S1)
Components
  • (DNA (28-MER)) x 2
  • DNA binding protein
KeywordsDNA BINDING PROTEIN / Halophiles / wHTH DNA binding protein / RosR / High salt medium / Protein-DNA interactions
Function / homologyTranscription regulator PadR, N-terminal / Transcriptional regulator PadR-like family / Winged helix DNA-binding domain superfamily / Winged helix-like DNA-binding domain superfamily / metal ion binding / : / DNA / DNA (> 10) / PadR family transcription regulator RosR
Function and homology information
Biological speciesHalobacterium salinarum (Halophile)
Halobacterium salinarum NRC-1 (Halophile)
MethodX-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 2 Å
AuthorsShaanan, B. / Kutnowski, N.
Funding support Israel, 1items
OrganizationGrant numberCountry
Israel Science Foundation1382/13 Israel
CitationJournal: Nucleic Acids Res. / Year: 2019
Title: Specificity of protein-DNA interactions in hypersaline environment: structural studies on complexes of Halobacterium salinarum oxidative stress-dependent protein hsRosR.
Authors: Kutnowski, N. / Shmulevich, F. / Davidov, G. / Shahar, A. / Bar-Zvi, D. / Eichler, J. / Zarivach, R. / Shaanan, B.
History
DepositionJan 21, 2019Deposition site: PDBE / Processing site: PDBE
Revision 1.0Jul 10, 2019Provider: repository / Type: Initial release
Revision 1.1Jul 24, 2019Group: Data collection / Database references / Category: citation / citation_author
Item: _citation.pdbx_database_id_PubMed / _citation.title ..._citation.pdbx_database_id_PubMed / _citation.title / _citation_author.identifier_ORCID / _citation_author.name
Revision 1.2Oct 23, 2019Group: Data collection / Database references / Category: citation
Item: _citation.journal_volume / _citation.page_first / _citation.page_last
Revision 1.3Jan 24, 2024Group: Data collection / Database references ...Data collection / Database references / Derived calculations / Refinement description
Category: chem_comp_atom / chem_comp_bond ...chem_comp_atom / chem_comp_bond / database_2 / pdbx_initial_refinement_model / struct_conn
Item: _database_2.pdbx_DOI / _database_2.pdbx_database_accession ..._database_2.pdbx_DOI / _database_2.pdbx_database_accession / _struct_conn.pdbx_dist_value / _struct_conn.pdbx_ptnr1_label_alt_id / _struct_conn.ptnr1_auth_asym_id / _struct_conn.ptnr1_auth_comp_id / _struct_conn.ptnr1_auth_seq_id / _struct_conn.ptnr1_label_asym_id / _struct_conn.ptnr1_label_atom_id / _struct_conn.ptnr1_label_comp_id / _struct_conn.ptnr1_label_seq_id / _struct_conn.ptnr2_auth_asym_id / _struct_conn.ptnr2_auth_comp_id / _struct_conn.ptnr2_auth_seq_id / _struct_conn.ptnr2_label_asym_id / _struct_conn.ptnr2_label_atom_id / _struct_conn.ptnr2_label_comp_id / _struct_conn.ptnr2_label_seq_id

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Assembly

Deposited unit
A: DNA binding protein
B: DNA binding protein
C: DNA binding protein
D: DNA binding protein
E: DNA (28-MER)
F: DNA (28-MER)
G: DNA (28-MER)
H: DNA (28-MER)
hetero molecules


Theoretical massNumber of molelcules
Total (without water)89,28034
Polymers87,1368
Non-polymers2,14426
Water1,838102
1
A: DNA binding protein
B: DNA binding protein
E: DNA (28-MER)
F: DNA (28-MER)
hetero molecules


  • defined by software
  • Evidence: SAXS, SAXS results indicating that one protein dimer bound a single sequence., gel filtration, The elution volume of the complex indicated that hsRosR binds one DNA molecule as a dimer., ...Evidence: SAXS, SAXS results indicating that one protein dimer bound a single sequence., gel filtration, The elution volume of the complex indicated that hsRosR binds one DNA molecule as a dimer., isothermal titration calorimetry, The binding stoichiometry was approximately one, indicating that one protein dimer bound a single sequence.
  • 45.1 kDa, 4 polymers
  • Search similar-shape structures of this assembly by Omokage search (details)
Theoretical massNumber of molelcules
Total (without water)45,06722
Polymers43,5684
Non-polymers1,49918
Water724
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area13130 Å2
ΔGint-202 kcal/mol
Surface area18430 Å2
MethodPISA
2
C: DNA binding protein
D: DNA binding protein
G: DNA (28-MER)
H: DNA (28-MER)
hetero molecules


Theoretical massNumber of molelcules
Total (without water)44,21312
Polymers43,5684
Non-polymers6458
Water724
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area11580 Å2
ΔGint-139 kcal/mol
Surface area18310 Å2
MethodPISA
Unit cell
Length a, b, c (Å)46.824, 84.304, 89.372
Angle α, β, γ (deg.)96.240, 93.750, 106.980
Int Tables number1
Space group name H-MP1

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Components

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Protein , 1 types, 4 molecules ABCD

#1: Protein
DNA binding protein


Mass: 13179.443 Da / Num. of mol.: 4
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Halobacterium salinarum (strain ATCC 700922 / JCM 11081 / NRC-1) (Halophile)
Strain: ATCC 700922 / JCM 11081 / NRC-1 / Gene: VNG_0258H / Production host: Haloferax volcanii (archaea) / Strain (production host): WR341 / References: UniProt: Q9HSF4

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DNA chain , 2 types, 4 molecules EGFH

#2: DNA chain DNA (28-MER)


Mass: 8666.585 Da / Num. of mol.: 2 / Source method: obtained synthetically / Source: (synth.) Halobacterium salinarum NRC-1 (Halophile)
#3: DNA chain DNA (28-MER)


Mass: 8542.545 Da / Num. of mol.: 2 / Source method: obtained synthetically / Source: (synth.) Halobacterium salinarum NRC-1 (Halophile)

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Non-polymers , 4 types, 128 molecules

#4: Chemical
ChemComp-MN / MANGANESE (II) ION


Mass: 54.938 Da / Num. of mol.: 11 / Source method: obtained synthetically / Formula: Mn
#5: Chemical
ChemComp-SO4 / SULFATE ION


Mass: 96.063 Da / Num. of mol.: 14 / Source method: obtained synthetically / Formula: SO4
#6: Chemical ChemComp-MES / 2-(N-MORPHOLINO)-ETHANESULFONIC ACID


Mass: 195.237 Da / Num. of mol.: 1 / Source method: obtained synthetically / Formula: C6H13NO4S / Comment: pH buffer*YM
#7: Water ChemComp-HOH / water


Mass: 18.015 Da / Num. of mol.: 102 / Source method: isolated from a natural source / Formula: H2O

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Experimental details

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Experiment

ExperimentMethod: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

CrystalDensity Matthews: 3.83 Å3/Da / Density % sol: 67.88 %
Crystal growTemperature: 293 K / Method: vapor diffusion, sitting drop / pH: 7
Details: The complex crystallized from a buffer containing 20 mM HEPES, pH 7, 2 M KCl and 0.02% azide. Crystals grown with S1 (DNA/hsRosR ratio = 1.46) appeared after 3 days in wells containing 2.77 ...Details: The complex crystallized from a buffer containing 20 mM HEPES, pH 7, 2 M KCl and 0.02% azide. Crystals grown with S1 (DNA/hsRosR ratio = 1.46) appeared after 3 days in wells containing 2.77 M (NH4)2SO4, 50 mM MnCl2 and 0.02% azide.

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Data collection

DiffractionMean temperature: 100 K / Serial crystal experiment: N
Diffraction sourceSource: SYNCHROTRON / Site: ESRF / Beamline: ID29 / Wavelength: 0.976 Å
DetectorType: DECTRIS PILATUS3 6M / Detector: PIXEL / Date: Sep 3, 2017
RadiationProtocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelengthWavelength: 0.976 Å / Relative weight: 1
ReflectionResolution: 1.88→44.548 Å / Num. obs: 57497 / % possible obs: 90.9 % / Redundancy: 3.5 % / Rpim(I) all: 0.029 / Net I/σ(I): 11.7
Reflection shellResolution: 1.88→2.124 Å

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Processing

Software
NameVersionClassification
PHENIXdev_3338refinement
PDB_EXTRACT3.24data extraction
XDSdata reduction
Aimlessdata scaling
PHASERphasing
STARANISOdata scaling
RefinementMethod to determine structure: MOLECULAR REPLACEMENT
Starting model: The starting model for molecular replacement was VNG0258H/RosR (from KCl; PDB code 6FDH) and a DNA model derived from Mycobacterium tuberculosis MosR (PDB code 4FX4) but with the S1 sequence.

6fdh

4fx4


Resolution: 2→44.548 Å / SU ML: 0.26 / Cross valid method: THROUGHOUT / σ(F): 1.96 / Phase error: 33.4
RfactorNum. reflection% reflection
Rfree0.235 2781 4.97 %
Rwork0.2075 --
obs0.2089 55939 64.04 %
Solvent computationShrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å
Displacement parametersBiso max: 174.85 Å2 / Biso mean: 66.5378 Å2 / Biso min: 11.32 Å2
Refinement stepCycle: final / Resolution: 2→44.548 Å
ProteinNucleic acidLigandSolventTotal
Num. atoms3659 2296 106 102 6163
Biso mean--84.03 46.75 -
Num. residues----569
LS refinement shell

Refine-ID: X-RAY DIFFRACTION / Rfactor Rfree error: 0 / Total num. of bins used: 20

Resolution (Å)Rfactor RfreeNum. reflection RfreeRfactor RworkNum. reflection RworkNum. reflection all% reflection obs (%)
2.0001-2.03460.3583210.31844146210
2.0346-2.07160.3231280.300761163915
2.0716-2.11150.338440.298978282619
2.1115-2.15460.288520.2917964101623
2.1546-2.20140.3168670.2971293136031
2.2014-2.25260.3177840.29411607169139
2.2526-2.3090.3471750.28141889196445
2.309-2.37140.3151170.28232094221151
2.3714-2.44120.28551550.28392388254358
2.4412-2.51990.31371500.27592707285765
2.5199-2.610.26871790.27852959313872
2.61-2.71450.3031890.28783334352381
2.7145-2.8380.33621880.30093825401393
2.838-2.98760.29532240.27194108433298
2.9876-3.17470.26692060.24884034424098
3.1747-3.41980.22712020.21864068427097
3.4198-3.76380.22842270.18924049427698
3.7638-4.3080.18632060.15864081428798
4.308-5.42610.19691690.16144042421197
5.4261-44.55910.18851980.16283882408093
Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

IDL11 (°2)L12 (°2)L13 (°2)L22 (°2)L23 (°2)L33 (°2)S11 (Å °)S12 (Å °)S13 (Å °)S21 (Å °)S22 (Å °)S23 (Å °)S31 (Å °)S32 (Å °)S33 (Å °)T11 (Å2)T12 (Å2)T13 (Å2)T22 (Å2)T23 (Å2)T33 (Å2)Origin x (Å)Origin y (Å)Origin z (Å)
13.20563.9098-3.16599.5112-2.13473.7636-0.1023-0.18380.45010.1261-0.2432-0.3809-0.15880.24650.3551.12270.2894-0.27471.0356-0.00210.7857-20.2816.2505-23.4692
26.1261-5.63611.83265.76150.97632.0083-0.1441.4466-0.0103-1.32370.19761.5053-0.3959-1.2452-0.03430.4763-0.1116-0.1760.61440.13030.4518-9.517216.624-18.1708
32.1876-1.18320.9175.4247-0.27871.1266-0.1087-0.27180.29680.27980.36220.045-0.3258-0.2288-0.23590.36270.05310.03520.28110.04890.4097-7.628821.2567-6.1706
43.4254.3259-3.90431.99821.98342.00230.3021-0.53240.4273-0.3943-0.1216-0.9259-0.30190.8728-0.18390.3757-0.09150.1250.38230.0170.47853.181720.0562-9.8397
53.6281-0.02430.710.34260.63521.51780.26040.46340.7183-1.07010.14350.5855-1.0057-0.391-0.32540.7120.0334-0.02950.44690.24910.6646-8.592126.3457-17.7449
65.42122.75510.40663.91880.67893.1683-0.2720.59111.2597-0.40240.24450.5071-1.0347-0.36830.03660.61020.1378-0.04490.47660.19590.7135-14.637327.9013-11.7426
73.2554-0.0812-0.99172.58881.75996.0313-0.18680.3429-0.0611-0.0538-0.01920.3380.3863-0.69910.20290.1654-0.0343-0.02330.3424-0.02850.3692-23.08992.3233-6.8708
82.01252.73984.47999.5093-6.87792.00280.0442-1.26011.33240.68670.39740.357-1.5115-0.1756-0.37280.36810.09620.04540.4898-0.10370.5598-20.677514.61676.4017
96.39871.3636-3.17425.07292.83985.57690.0319-0.5779-0.41950.36790.4438-0.87060.62041.0688-0.48790.33710.0762-0.09120.3325-0.04590.3216-4.61594.6886-11.6576
103.0463-2.7466-1.84925.49271.36714.1971-0.07150.5055-0.4686-0.4539-0.11260.72560.4974-0.79840.10270.4208-0.1005-0.03460.4448-0.03140.3029-15.5983-2.2551-22.0735
119.0935-1.7121.61849.16124.24738.73930.39281.2384-0.3464-0.9235-0.00530.4047-0.3313-1.1543-0.37210.5584-0.001-0.05830.6385-0.03140.3238-17.13386.0356-28.1139
122.77020.1616-2.37825.5624-2.18318.4275-0.12630.4007-0.0807-0.8128-0.0245-0.6160.33950.62240.15020.3756-0.00280.08340.4561-0.09080.3086-4.6367-1.2898-23.4703
136.59012.8833.22439.21195.61422.0048-0.20960.1589-0.9005-0.6184-0.64470.72861.5984-0.11070.81270.7416-0.01450.08430.4969-0.12490.6146-9.5046-14.3149-25.7249
142.6613-0.7988-1.66381.88160.62972.0441-0.0092-0.11080.45270.01010.2538-0.21090.1015-0.2906-0.26870.18890.0186-0.0310.3249-0.01190.3125-13.93125.5781-7.3169
150.46291.00080.30192.85970.08290.7469-0.00580.2481.0397-0.1674-0.01981.0271-0.086-0.59020.01060.28640.0423-0.00120.8520.10290.6625-28.591612.4758-5.2526
167.9991.96421.71485.49812.71582.00750.40261.1618-1.9738-0.48470.20190.3810.7122-0.6889-0.58770.4653-0.2377-0.14110.8892-0.05460.7989-32.51630.4282-12.8149
179.9316.4178-4.18596.71920.73696.38180.03850.75031.1536-0.97710.36750.8614-0.023-0.5617-0.40490.4813-0.00330.00480.55850.05580.3184-9.768810.776315.8338
189.42530.5042-0.44628.79961.86684.7664-0.09930.0412-0.25320.75470.2837-0.58360.52170.1074-0.1650.24140.0125-0.0460.19050.03210.2165-4.0432-0.104510.742
198.36142.89623.43285.89263.34335.6975-0.0855-0.5431-0.9680.79150.1128-0.74630.71850.4841-0.07380.45070.0622-0.08490.34580.08880.4229-1.8371-8.723414.7449
203.34620.89870.06466.9586-0.67454.97030.1722-1.0343-0.58061.0339-0.38920.22820.7843-0.29550.25210.4598-0.08160.04940.4690.09230.2963-12.2092-4.965717.1154
212.05255.1742.30942.03285.92277.46990.10820.55870.09670.1458-0.25471.2960.6912-1.01860.01360.3208-0.0478-0.01370.4462-0.02650.399-16.0656-0.7226.7474
227.3132-2.9398-0.86792.0053-2.73577.0103-0.29510.6593-0.2354-0.62720.58150.33871.1274-0.3807-0.24970.633-0.2117-0.04860.4735-0.08370.5201-14.7104-14.0694.2186
232.51280.7791-0.92054.03310.7312.29590.0542-0.18050.89250.10290.1923-0.0764-0.08290.1249-0.23740.18310.0604-0.01180.2774-0.01760.31144.364411.44767.6404
246.9840.3561-0.71788.2153-4.42657.0407-0.01650.7644-1.13090.31190.7066-0.66460.83950.991-0.69670.26410.1307-0.06570.3994-0.1610.486113.1002-1.31490.2933
254.62590.8383-0.45374.04830.95854.31030.1941-0.60110.13660.47640.001-0.5947-0.05680.3077-0.13250.3928-0.0023-0.11720.5644-0.15290.41673.307614.927122.9984
266.66690.70174.94689.3165-0.06276.58640.2445-0.43980.75250.7389-0.28750.7706-0.4154-0.5776-0.04090.42640.07420.0550.5708-0.14850.4868-7.486220.318919.2448
272.65021.5331.941.88060.6676.0570.1617-0.7280.5440.81250.2947-0.19790.1341-0.1216-0.40260.6226-0.11690.04180.8335-0.25830.4908-4.637517.908230.7877
281.0933-0.7645-0.18694.52720.46025.85020.2827-0.7154-0.0090.7757-0.1745-0.11180.27610.0131-0.08330.71350.0167-0.28311.0155-0.15840.47916.771811.63431.8496
290.0094-0.3043-0.24418.44627.12956.00390.2561-0.52820.52110.73810.1217-0.3809-0.38021.1212-0.36280.9359-0.203-0.09211.0594-0.42420.78278.487525.225934.3417
303.9019-2.14410.83086.49052.57877.5976-0.1519-1.21260.36861.2111-0.0706-0.90930.62220.44260.14710.43720.0448-0.14650.5753-0.06670.50311.95648.766721.1896
312.26240.5251-0.7913.2217-0.55133.29030.0012-0.09370.1867-0.0477-0.0428-0.37850.02590.20150.03720.18190.0734-0.02350.25050.0050.40095.69366.18172.723
322.0081-3.2501-0.4537.48971.16718.7662-0.0804-0.31921.73790.29360.0784-0.3603-1.28990.2988-0.00490.4143-0.0183-0.05340.3957-0.07030.84379.357222.74344.1107
336.0013-0.80182.23060.99991.49384.4266-0.44550.22840.66620.2946-0.1772-1.16-1.22920.7060.56171.4259-0.51550.03850.636-0.41041.781511.153841.47760.0689
344.31680.0891-3.13382.6107-2.43132.00330.28040.38510.6862-0.93610.5065-0.3754-0.94940.2483-0.7940.7928-0.09760.1140.40250.0670.76822.56632.7679-12.9132
353.9654-1.83120.82323.5505-4.18135.55930.81780.5135-0.0857-1.6436-0.1698-1.27340.10730.4275-0.61150.9495-0.06340.34180.7402-0.0590.54973.519212.7009-29.5152
361.76340.55970.6574.1938-0.08741.8460.12720.8484-0.5922-1.2319-0.3009-0.3511-0.1953-0.3720.10281.4476-0.10260.23310.9799-0.45320.8208-8.0594-13.6939-40.1366
377.87444.364.6427.2297-2.91518.9812-0.02871.1588-0.0238-0.5351-0.4074-1.4772-0.4093-0.41910.37381.71250.01710.03281.0744-0.54011.0323-13.3626-15.2859-47.4425
382.1620.2062-1.99035.0949-0.90314.3145-0.24650.5642-0.3715-1.4533-0.1491-0.4197-0.5044-0.03050.42580.9184-0.10380.19060.7889-0.23670.5039-5.168-6.4463-34.2431
395.1151-4.6921-2.72664.53631.19429.8198-0.24780.91130.4065-1.66490.3075-1.5615-1.00720.0606-0.2180.7921-0.24210.29520.6030.04920.69654.679216.2512-26.9613
401.8307-1.5042-1.38025.32860.99572.53030.7353-0.26420.37850.41940.1044-1.1765-1.02780.5114-0.77611.242-0.28310.01270.50260.00041.23475.646539.7952-6.9833
412.7964-0.28181.12113.70570.09981.51490.4222-0.5060.18080.76070.26550.02460.0186-0.2143-0.25491.5569-0.3445-0.00871.159-1.55571.2103-5.715240.941840.3779
423.00830.1327-0.25961.69-0.07082.36250.3049-0.840.35880.8423-0.11050.08030.09-0.3997-0.18310.9672-0.1810.0771.0393-0.38060.6624-4.438322.066638.395
436.38361.0503-0.11121.2558-1.74892.75520.8239-1.6234-0.45810.5008-0.46110.20790.9287-0.9714-0.31761.079-0.2373-0.02810.97490.06810.3689-15.0916-1.903329.9034
440.63020.21341.67341.57290.6724.42680.06860.0868-0.80740.8896-0.1666-0.47321.2529-0.85950.02731.0446-0.34380.09790.5876-0.09321.0332-17.6457-23.276410.7021
452.8043-3.11271.14263.4611-1.30077.8480.0707-0.4273-1.90470.5715-0.4690.38240.9145-0.73060.2552.128-0.453-0.06060.4815-0.06291.7093-14.588-31.557212.5438
464.24531.5492.35212.7062-0.45866.70440.289-1.287-0.34051.4717-0.41830.28930.7328-1.05530.07650.9288-0.23250.12060.68410.06120.5356-17.0955-11.450621.8898
472.04950.6494-0.95480.32-1.54338.32990.2821-1.89280.76981.4667-0.5672-0.0840.3659-1.11090.24450.6918-0.20030.01021.0284-0.22220.592-11.724110.46133.3475
481.95970.85550.96862.31530.89990.59950.5424-0.60720.2120.3717-0.65930.2127-0.4151-0.72480.15481.4888-0.19990.15471.2614-0.57550.8595-1.971632.81141.2042
Refinement TLS group
IDRefine-IDRefine TLS-IDSelection detailsAuth asym-IDAuth seq-ID
1X-RAY DIFFRACTION1(chain A and resid 7:13)A7 - 13
2X-RAY DIFFRACTION2(chain A and resid 14:20)A14 - 20
3X-RAY DIFFRACTION3(chain A and resid 21:44)A21 - 44
4X-RAY DIFFRACTION4(chain A and resid 45:51)A45 - 51
5X-RAY DIFFRACTION5(chain A and resid 52:67)A52 - 67
6X-RAY DIFFRACTION6(chain A and resid 68:92)A68 - 92
7X-RAY DIFFRACTION7(chain A and resid 93:115)A93 - 115
8X-RAY DIFFRACTION8(chain A and resid 116:121)A116 - 121
9X-RAY DIFFRACTION9(chain B and resid 6:19)B6 - 19
10X-RAY DIFFRACTION10(chain B and resid 20:38)B20 - 38
11X-RAY DIFFRACTION11(chain B and resid 39:48)B39 - 48
12X-RAY DIFFRACTION12(chain B and resid 49:66)B49 - 66
13X-RAY DIFFRACTION13(chain B and resid 67:77)B67 - 77
14X-RAY DIFFRACTION14(chain B and resid 78:103)B78 - 103
15X-RAY DIFFRACTION15(chain B and resid 104:114)B104 - 114
16X-RAY DIFFRACTION16(chain B and resid 115:121)B115 - 121
17X-RAY DIFFRACTION17(chain C and resid 10:16)C10 - 16
18X-RAY DIFFRACTION18(chain C and resid 17:30)C17 - 30
19X-RAY DIFFRACTION19(chain C and resid 31:47)C31 - 47
20X-RAY DIFFRACTION20(chain C and resid 48:60)C48 - 60
21X-RAY DIFFRACTION21(chain C and resid 61:70)C61 - 70
22X-RAY DIFFRACTION22(chain C and resid 71:77)C71 - 77
23X-RAY DIFFRACTION23(chain C and resid 78:114)C78 - 114
24X-RAY DIFFRACTION24(chain C and resid 115:121)C115 - 121
25X-RAY DIFFRACTION25(chain D and resid 8:40)D8 - 40
26X-RAY DIFFRACTION26(chain D and resid 41:47)D41 - 47
27X-RAY DIFFRACTION27(chain D and resid 48:56)D48 - 56
28X-RAY DIFFRACTION28(chain D and resid 57:67)D57 - 67
29X-RAY DIFFRACTION29(chain D and resid 68:79)D68 - 79
30X-RAY DIFFRACTION30(chain D and resid 80:91)D80 - 91
31X-RAY DIFFRACTION31(chain D and resid 92:112)D92 - 112
32X-RAY DIFFRACTION32(chain D and resid 113:121)D113 - 121
33X-RAY DIFFRACTION33(chain E and resid 1:4)E1 - 4
34X-RAY DIFFRACTION34(chain E and resid 5:10)E5 - 10
35X-RAY DIFFRACTION35(chain E and resid 11:20)E11 - 20
36X-RAY DIFFRACTION36(chain E and resid 21:28)E21 - 28
37X-RAY DIFFRACTION37(chain F and resid 1:4)F1 - 4
38X-RAY DIFFRACTION38(chain F and resid 5:10)F5 - 10
39X-RAY DIFFRACTION39(chain F and resid 11:20)F11 - 20
40X-RAY DIFFRACTION40(chain F and resid 21:28)F21 - 28
41X-RAY DIFFRACTION41(chain G and resid 1:4)G1 - 4
42X-RAY DIFFRACTION42(chain G and resid 5:11)G5 - 11
43X-RAY DIFFRACTION43(chain G and resid 12:21)G12 - 21
44X-RAY DIFFRACTION44(chain G and resid 22:28)G22 - 28
45X-RAY DIFFRACTION45(chain H and resid 1:4)H1 - 4
46X-RAY DIFFRACTION46(chain H and resid 5:13)H5 - 13
47X-RAY DIFFRACTION47(chain H and resid 14:20)H14 - 20
48X-RAY DIFFRACTION48(chain H and resid 21:28)H21 - 28

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