Entry | Database: PDB / ID: 6pxu |
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Title | Crystal structure of human GalNAc-T12 bound to a diglycosylated peptide, Mn2+, and UDP |
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Components | - GAGATGAGAGYYITPRTGAGA
- Polypeptide N-acetylgalactosaminyltransferase 12
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Keywords | TRANSFERASE / GalNAc-T / mucin-type O-glycosylation / enzyme catalysis / substrate selectivity / colorectal cancer / CRC |
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Function / homology | Function and homology information
polypeptide N-acetylgalactosaminyltransferase / polypeptide N-acetylgalactosaminyltransferase activity / Defective GALNT12 causes CRCS1 / O-glycan processing / O-linked glycosylation of mucins / carbohydrate binding / membrane => GO:0016020 / Golgi membrane / Golgi apparatus / metal ion bindingSimilarity search - Function Glycosyltransferase 2-like / Glycosyl transferase family 2 / Ricin-type beta-trefoil lectin domain / Ricin-type beta-trefoil / Lectin domain of ricin B chain profile. / Ricin B, lectin domain / Ricin B-like lectins / Nucleotide-diphospho-sugar transferasesSimilarity search - Domain/homology 2-acetamido-2-deoxy-alpha-D-galactopyranose / : / URIDINE-5'-DIPHOSPHATE / Polypeptide N-acetylgalactosaminyltransferase 12Similarity search - Component |
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Biological species | Homo sapiens (human) synthetic construct (others) |
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Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 2.007 Å |
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Authors | Samara, N.L. / Fernandez, A.J. |
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Funding support | United States, 2items Organization | Grant number | Country |
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National Institutes of Health/National Human Genome Research Institute (NIH/NHGRI) | 1 ZIA DE000739 05 | United States | National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS) | R01 GM113534 | United States |
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Citation | Journal: Proc.Natl.Acad.Sci.USA / Year: 2019 Title: The structure of the colorectal cancer-associated enzyme GalNAc-T12 reveals how nonconserved residues dictate its function. Authors: Fernandez, A.J. / Daniel, E.J.P. / Mahajan, S.P. / Gray, J.J. / Gerken, T.A. / Tabak, L.A. / Samara, N.L. |
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History | Deposition | Jul 27, 2019 | Deposition site: RCSB / Processing site: RCSB |
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Revision 1.0 | Sep 25, 2019 | Provider: repository / Type: Initial release |
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Revision 1.1 | Oct 9, 2019 | Group: Data collection / Database references / Category: citation / Item: _citation.pdbx_database_id_PubMed / _citation.title |
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Revision 1.2 | Oct 23, 2019 | Group: Data collection / Database references / Category: citation Item: _citation.journal_volume / _citation.page_first / _citation.page_last |
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Revision 1.3 | Dec 18, 2019 | Group: Author supporting evidence / Category: pdbx_audit_support / Item: _pdbx_audit_support.funding_organization |
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Revision 2.0 | Jul 29, 2020 | Group: Advisory / Atomic model ...Advisory / Atomic model / Data collection / Derived calculations / Structure summary Category: atom_site / chem_comp ...atom_site / chem_comp / entity / pdbx_chem_comp_identifier / pdbx_entity_nonpoly / pdbx_struct_conn_angle / pdbx_validate_close_contact / struct_conn / struct_conn_type / struct_site / struct_site_gen Item: _atom_site.auth_atom_id / _atom_site.label_atom_id ..._atom_site.auth_atom_id / _atom_site.label_atom_id / _chem_comp.name / _chem_comp.type / _entity.pdbx_description / _pdbx_entity_nonpoly.name / _pdbx_struct_conn_angle.ptnr1_auth_comp_id / _pdbx_struct_conn_angle.ptnr1_auth_seq_id / _pdbx_struct_conn_angle.ptnr1_label_asym_id / _pdbx_struct_conn_angle.ptnr1_label_atom_id / _pdbx_struct_conn_angle.ptnr1_label_comp_id / _pdbx_struct_conn_angle.ptnr3_auth_comp_id / _pdbx_struct_conn_angle.ptnr3_auth_seq_id / _pdbx_struct_conn_angle.ptnr3_label_asym_id / _pdbx_struct_conn_angle.ptnr3_label_atom_id / _pdbx_struct_conn_angle.ptnr3_label_comp_id / _pdbx_struct_conn_angle.value / _pdbx_validate_close_contact.auth_atom_id_2 / _struct_conn.conn_type_id / _struct_conn.id / _struct_conn.pdbx_dist_value / _struct_conn.pdbx_leaving_atom_flag / _struct_conn.pdbx_role / _struct_conn.ptnr1_auth_asym_id / _struct_conn.ptnr1_auth_comp_id / _struct_conn.ptnr1_auth_seq_id / _struct_conn.ptnr1_label_asym_id / _struct_conn.ptnr1_label_atom_id / _struct_conn.ptnr1_label_comp_id / _struct_conn.ptnr1_label_seq_id / _struct_conn.ptnr1_symmetry / _struct_conn.ptnr2_auth_asym_id / _struct_conn.ptnr2_auth_comp_id / _struct_conn.ptnr2_auth_seq_id / _struct_conn.ptnr2_label_asym_id / _struct_conn.ptnr2_label_atom_id / _struct_conn.ptnr2_label_comp_id / _struct_conn.ptnr2_label_seq_id / _struct_conn.ptnr2_symmetry / _struct_conn_type.id Description: Carbohydrate remediation / Provider: repository / Type: Remediation |
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Revision 2.1 | Oct 11, 2023 | Group: Data collection / Database references ...Data collection / Database references / Refinement description / Structure summary Category: chem_comp / chem_comp_atom ...chem_comp / chem_comp_atom / chem_comp_bond / database_2 / pdbx_initial_refinement_model Item: _chem_comp.pdbx_synonyms / _database_2.pdbx_DOI / _database_2.pdbx_database_accession |
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