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- PDB-6pxk: 3.65 Angstroms resolution structure of HslU with an axial-channel plug -

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Basic information

Entry
Database: PDB / ID: 6pxk
Title3.65 Angstroms resolution structure of HslU with an axial-channel plug
Components
  • ATP-dependent protease ATPase subunit HslU
  • unidentified alpha helical sequence
KeywordsHYDROLASE / AAA+ ATPase / peptidase
Function / homology
Function and homology information


protein denaturation / HslUV protease complex / proteasome-activating activity / protein unfolding / proteolysis involved in protein catabolic process / peptidase activity / cellular response to heat / response to heat / protein domain specific binding / magnesium ion binding ...protein denaturation / HslUV protease complex / proteasome-activating activity / protein unfolding / proteolysis involved in protein catabolic process / peptidase activity / cellular response to heat / response to heat / protein domain specific binding / magnesium ion binding / ATP hydrolysis activity / proteolysis / ATP binding / identical protein binding / membrane / cytosol
Similarity search - Function
Heat shock protein HslU / : / Helicase, Ruva Protein; domain 3 - #60 / Clp ATPase, C-terminal / C-terminal, D2-small domain, of ClpB protein / C-terminal, D2-small domain, of ClpB protein / AAA domain (Cdc48 subfamily) / Helicase, Ruva Protein; domain 3 / ATPase family associated with various cellular activities (AAA) / ATPase, AAA-type, core ...Heat shock protein HslU / : / Helicase, Ruva Protein; domain 3 - #60 / Clp ATPase, C-terminal / C-terminal, D2-small domain, of ClpB protein / C-terminal, D2-small domain, of ClpB protein / AAA domain (Cdc48 subfamily) / Helicase, Ruva Protein; domain 3 / ATPase family associated with various cellular activities (AAA) / ATPase, AAA-type, core / ATPases associated with a variety of cellular activities / AAA+ ATPase domain / P-loop containing nucleoside triphosphate hydrolase / Orthogonal Bundle / Mainly Alpha
Similarity search - Domain/homology
ADENOSINE-5'-DIPHOSPHATE / ATP-dependent protease ATPase subunit HslU / ATP-dependent protease ATPase subunit HslU
Similarity search - Component
Biological speciesEscherichia coli (E. coli)
MethodX-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 3.647 Å
AuthorsBaytshtok, V. / Grant, R.A. / Sauer, R.T.
Funding support United States, 1items
OrganizationGrant numberCountry
National Institutes of Health/National Human Genome Research Institute (NIH/NHGRI) United States
CitationJournal: Cell Rep / Year: 2021
Title: Heat activates the AAA+ HslUV protease by melting an axial autoinhibitory plug.
Authors: Baytshtok, V. / Fei, X. / Shih, T.T. / Grant, R.A. / Santos, J.C. / Baker, T.A. / Sauer, R.T.
History
DepositionJul 26, 2019Deposition site: RCSB / Processing site: RCSB
Revision 1.0Jul 29, 2020Provider: repository / Type: Initial release
Revision 1.1Oct 14, 2020Group: Data collection / Category: diffrn_radiation_wavelength / diffrn_source
Item: _diffrn_radiation_wavelength.wavelength / _diffrn_source.pdbx_wavelength_list
Revision 1.2Feb 10, 2021Group: Database references / Category: citation / citation_author
Item: _citation.country / _citation.journal_abbrev ..._citation.country / _citation.journal_abbrev / _citation.journal_id_CSD / _citation.journal_id_ISSN / _citation.journal_volume / _citation.page_first / _citation.page_last / _citation.pdbx_database_id_DOI / _citation.pdbx_database_id_PubMed / _citation.title / _citation.year
Revision 1.3Mar 16, 2022Group: Author supporting evidence / Database references / Category: database_2 / pdbx_audit_support
Item: _database_2.pdbx_DOI / _database_2.pdbx_database_accession / _pdbx_audit_support.funding_organization
Revision 1.4Oct 11, 2023Group: Data collection / Refinement description
Category: chem_comp_atom / chem_comp_bond / pdbx_initial_refinement_model
Revision 1.5Nov 15, 2023Group: Data collection / Category: chem_comp_atom / chem_comp_bond / Item: _chem_comp_atom.atom_id / _chem_comp_bond.atom_id_2
Revision 1.6Nov 6, 2024Group: Structure summary / Category: pdbx_entry_details / pdbx_modification_feature / Item: _pdbx_entry_details.has_protein_modification

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Assembly

Deposited unit
G: ATP-dependent protease ATPase subunit HslU
H: ATP-dependent protease ATPase subunit HslU
I: ATP-dependent protease ATPase subunit HslU
J: ATP-dependent protease ATPase subunit HslU
K: ATP-dependent protease ATPase subunit HslU
L: ATP-dependent protease ATPase subunit HslU
A: ATP-dependent protease ATPase subunit HslU
B: ATP-dependent protease ATPase subunit HslU
C: ATP-dependent protease ATPase subunit HslU
D: ATP-dependent protease ATPase subunit HslU
E: ATP-dependent protease ATPase subunit HslU
F: ATP-dependent protease ATPase subunit HslU
X: unidentified alpha helical sequence
hetero molecules


Theoretical massNumber of molelcules
Total (without water)619,64741
Polymers612,98413
Non-polymers6,66328
Water00
1
A: ATP-dependent protease ATPase subunit HslU
B: ATP-dependent protease ATPase subunit HslU
C: ATP-dependent protease ATPase subunit HslU
D: ATP-dependent protease ATPase subunit HslU
E: ATP-dependent protease ATPase subunit HslU
F: ATP-dependent protease ATPase subunit HslU
X: unidentified alpha helical sequence
hetero molecules


Theoretical massNumber of molelcules
Total (without water)310,32119
Polymers307,1827
Non-polymers3,14012
Water0
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
2
G: ATP-dependent protease ATPase subunit HslU
H: ATP-dependent protease ATPase subunit HslU
I: ATP-dependent protease ATPase subunit HslU
J: ATP-dependent protease ATPase subunit HslU
K: ATP-dependent protease ATPase subunit HslU
L: ATP-dependent protease ATPase subunit HslU
hetero molecules


Theoretical massNumber of molelcules
Total (without water)309,32622
Polymers305,8026
Non-polymers3,52416
Water0
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area29850 Å2
ΔGint-96 kcal/mol
Surface area100960 Å2
MethodPISA
Unit cell
Length a, b, c (Å)200.030, 91.200, 201.800
Angle α, β, γ (deg.)90.000, 99.430, 90.000
Int Tables number3
Space group name H-MP121

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Components

#1: Protein
ATP-dependent protease ATPase subunit HslU / Heat shock protein HslU / Unfoldase HslU


Mass: 50967.012 Da / Num. of mol.: 12
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Escherichia coli (E. coli) / Gene: hslU / Production host: Escherichia coli (E. coli) / References: UniProt: C3SIX7, UniProt: P0A6H5*PLUS
#2: Protein/peptide unidentified alpha helical sequence


Mass: 1379.692 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Escherichia coli (E. coli) / Gene: HslU / Production host: Escherichia coli (E. coli)
#3: Chemical
ChemComp-ADP / ADENOSINE-5'-DIPHOSPHATE


Mass: 427.201 Da / Num. of mol.: 12 / Source method: obtained synthetically / Formula: C10H15N5O10P2 / Comment: ADP, energy-carrying molecule*YM
#4: Chemical
ChemComp-SO4 / SULFATE ION


Mass: 96.063 Da / Num. of mol.: 16 / Source method: obtained synthetically / Formula: SO4
Has ligand of interestN
Has protein modificationY

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Experimental details

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Experiment

ExperimentMethod: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

CrystalDensity Matthews: 2.96 Å3/Da / Density % sol: 58.49 %
Crystal growTemperature: 298 K / Method: vapor diffusion, hanging drop
Details: 0.1 M ammonium sulfate, 18% PEG-3350, 0.1 M TRIS pH 7.5

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Data collection

DiffractionMean temperature: 100 K / Serial crystal experiment: N
Diffraction sourceSource: SYNCHROTRON / Site: APS / Beamline: 24-ID-C / Wavelength: 0.9792 Å
DetectorType: DECTRIS PILATUS3 S 6M / Detector: PIXEL / Date: Aug 3, 2016
RadiationProtocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelengthWavelength: 0.9792 Å / Relative weight: 1
ReflectionResolution: 3.647→47.687 Å / Num. obs: 154279 / % possible obs: 98.4 % / Redundancy: 5.01 % / Biso Wilson estimate: 145.18 Å2 / CC1/2: 0.998 / Rmerge(I) obs: 0.105 / Rrim(I) all: 0.117 / Χ2: 1.363 / Net I/σ(I): 8.9
Reflection shell

Diffraction-ID: 1

Resolution (Å)Redundancy (%)Rmerge(I) obsMean I/σ(I) obsNum. measured obsNum. possibleNum. unique obsCC1/2Rrim(I) all% possible all
3.65-3.744.6891.2871.024989111504106410.6091.4492.5
3.74-3.844.7821.0041.345340411310111680.6911.12998.7
3.84-3.965.0270.6942.035511211055109630.8220.77599.2
3.96-4.085.2630.5182.775545010656105360.8970.57498.9
4.08-4.215.2150.43.535353110319102650.9430.44599.5
4.21-4.365.1360.3054.685148610112100250.9540.3499.1
4.36-4.525.0760.2276.2448465963695480.9740.25299.1
4.52-4.714.7920.1857.4543945926591700.9790.20799
4.71-4.924.8620.1668.342657894087740.980.18698.1
4.92-5.165.2470.1589.2544335850184500.9840.17599.4
5.16-5.445.1910.1499.6841774808680470.9840.16599.5
5.44-5.775.1190.1439.9339230771476630.9840.15999.3
5.77-6.164.970.12910.6735308719071040.9870.14498.8
6.16-6.664.9190.113.6132301671165670.9910.11297.9
6.66-7.295.2220.07618.4731858611861010.9950.08499.7
7.29-8.165.0250.05822.6727945561455610.9970.06599.1
8.16-9.424.6350.0525.0922124490147730.9970.05697.4
9.42-11.534.9390.04528.0120392416841290.9980.0599.1
11.53-16.315.0070.04128.9915733318931420.9990.04698.5
16.31-47.6874.850.04230.188013177816520.9970.04792.9

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Processing

Software
NameVersionClassification
HKL-2000data reduction
XSCALEdata scaling
PHENIX1.13_2998refinement
PDB_EXTRACT3.24data extraction
PHASERphasing
RefinementMethod to determine structure: MOLECULAR REPLACEMENT
Starting model: 1DO0

1do0


Resolution: 3.647→47.687 Å / SU ML: 0.51 / Cross valid method: THROUGHOUT / σ(F): 1.36 / Phase error: 29.06
RfactorNum. reflection% reflection
Rfree0.2705 3887 4.88 %
Rwork0.2297 --
obs0.2317 79577 98.64 %
Solvent computationShrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å
Displacement parametersBiso max: 375.27 Å2 / Biso mean: 162.2472 Å2 / Biso min: 86.4 Å2
Refinement stepCycle: final / Resolution: 3.647→47.687 Å
ProteinNucleic acidLigandSolventTotal
Num. atoms37119 0 404 0 37523
Biso mean--145.07 --
Num. residues----4728
LS refinement shell

Refine-ID: X-RAY DIFFRACTION / Rfactor Rfree error: 0

Resolution (Å)Rfactor RfreeNum. reflection RfreeRfactor RworkNum. reflection Rwork% reflection obs (%)
3.6472-3.69160.36431260.3479236488
3.6916-3.73840.32881160.3344269299
3.7384-3.78750.3351310.3273272099
3.7875-3.83940.37621280.3105267699
3.8394-3.89420.34441520.298267399
3.8942-3.95230.3031260.2861274299
3.9523-4.0140.26041260.2837265399
4.014-4.07980.321440.2746269599
4.0798-4.15010.34571410.2647275599
4.1501-4.22550.29121130.2617267499
4.2255-4.30680.30461380.2614272799
4.3068-4.39460.28891560.2525267199
4.3946-4.49010.27571610.235267499
4.4901-4.59450.26471320.2331274399
4.5945-4.70930.27921420.2311267899
4.7093-4.83650.26981470.2199269299
4.8365-4.97870.25391280.2222271099
4.9787-5.13920.26081480.2326272299
5.1392-5.32260.26231450.23282730100
5.3226-5.53540.27371520.2488269199
5.5354-5.78690.30191330.2623275899
5.7869-6.09150.29711460.2679271499
6.0915-6.47220.26621550.2527269298
6.4722-6.97060.27231400.23122739100
6.9706-7.66940.25611520.21392744100
7.6694-8.77320.20931430.173275399
8.7732-11.03070.21511360.1655275398
11.0307-47.6870.28311300.2149285598
Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

IDL11 (°2)L12 (°2)L13 (°2)L22 (°2)L23 (°2)L33 (°2)S11 (Å °)S12 (Å °)S13 (Å °)S21 (Å °)S22 (Å °)S23 (Å °)S31 (Å °)S32 (Å °)S33 (Å °)T11 (Å2)T12 (Å2)T13 (Å2)T22 (Å2)T23 (Å2)T33 (Å2)Origin x (Å)Origin y (Å)Origin z (Å)
13.47631.182-0.09565.40210.73893.6841-0.06730.0656-0.184-0.25350.1966-0.3753-0.45710.21930.00431.0938-0.1360.03261.3056-0.22621.3391-38.2313.512168.1618
21.6746-0.66990.15241.15950.97076.15330.17160.1121-0.0824-0.4106-0.0419-0.5384-0.92090.5377-0.04261.1687-0.17190.21811.2913-0.21311.3721-41.03772.743136.7582
33.399-2.0243-1.37393.6959-0.42644.47390.02680.30270.1165-0.2388-0.0037-0.0005-0.4646-0.4971-0.04511.64840.127-0.01071.8133-0.3161.3909-67.9363-3.473123.0052
43.24230.84060.92132.1971-0.90174.9685-0.11480.7667-0.4410.01590.00560.2036-0.1692-1.0570.14320.95850.14270.05291.5352-0.33911.585-93.1674-8.805940.7451
53.81950.4647-0.34295.40390.65064.3652-0.0412-0.1557-0.59190.8174-0.02180.1647-0.0471-0.24880.10210.83150.0784-0.04580.9912-0.05071.1205-91.408-7.755771.4974
64.3086-0.5057-0.77984.12640.06334.2619-0.15490.0619-0.0581-0.15020.1496-0.2725-0.3680.1063-0.03891.1104-0.01790.09580.8164-0.07980.9991-63.8103-1.335484.9441
75.5661.75791.17856.1999-1.81094.9168-0.39280.1148-0.12020.21190.04990.0442-0.6217-0.4920.15491.01060.2608-0.17351.0266-0.19861.07381.088743.766849.3139
83.7348-0.6576-0.88324.13411.62753.7746-0.0550.0381-0.0476-0.6472-0.23520.1147-0.69320.00130.15581.5930.0808-0.19791.2984-0.00591.201613.137340.211420.3531
94.4439-1.5309-0.84624.69460.00633.12760.01-0.0892-0.1824-0.3893-0.26680.10570.25030.13550.16741.3051-0.00660.07041.06060.18541.002541.240227.913615.4612
104.8997-0.83541.78283.79520.66246.02990.0850.37340.1106-0.4198-0.212-0.32850.25020.72230.13391.26910.08530.16241.33720.23241.139957.558818.258740.8186
114.23840.8295-0.98552.02571.28416.91310.00870.121-0.10360.0949-0.0113-0.1215-0.09480.2275-0.05311.13270.08260.03411.01180.17741.25146.239921.355369.6136
124.4327-0.2561-0.65611.91190.40445.4534-0.1513-0.0163-0.1340.0924-0.1170.2132-0.27990.02980.17611.01390.0485-0.06491.01390.06181.217617.674434.161873.355
130.3845-0.1064-0.63240.00790.39010.82740.47911.0651-0.4416-0.2151-0.0524-0.85390.40460.1023-0.23191.67320.3087-0.1572.6172-0.6442.6961-39.178-28.21450.0634
14-2.0569-1.0139-0.95893.00072.7948-0.305-0.24940.17380.2437-0.62310.03640.1875-0.0716-0.07210.2582.62810.2282-0.18251.8117-0.38731.7894-47.8708-34.644926.5154
15-0.46020.74730.22351.067-0.42671.0789-0.2096-0.0422-0.5145-0.37860.4822-0.2450.6273-0.6323-0.0561.68870.0743-0.12192.0977-0.29132.1686-72.0347-37.326336.6244
16-0.23440.1621-0.37090.2822-0.8081.3485-0.0937-1.3290.2387-1.08611.08420.74151.4683-1.064-0.04521.4872-0.05230.12342.30630.23182.918-85.2847-30.274851.0525
17-0.0013-0.01370.14642.7003-0.97580.7748-0.0456-0.5833-0.42040.3499-0.3661-0.46510.45770.810.30941.62450.18220.10241.69680.12311.5705-66.7809-32.396378.1944
18-0.4841-1.7135-0.09620.83251.29821.58-0.68360.1309-0.3460.54420.4449-0.23250.66070.60030.07092.12580.2356-0.08421.4369-0.09362.5297-39.8206-29.708782.5279
195.4079-1.38950.28350.53521.38092.4651-0.23261.38760.9729-0.41850.2978-0.0705-0.96341.3278-0.38471.9858-0.2125-0.16341.67230.19951.74622.793762.386841.3281
202.29212.2083-1.6825-0.4052-1.6727-0.05550.45780.00270.24810.1006-0.35570.3884-0.29240.0756-0.13921.8378-0.26260.17411.60480.11071.570742.832964.744516.0453
211.4886-0.5144-0.71141.8346-0.76641.90190.4383-0.86470.77250.4475-0.0692-0.5127-0.11070.6053-0.33811.7813-0.19670.05291.7727-0.03771.94558.802152.563932.847
221.53290.09390.45451.06180.73570.6472-0.46010.9160.6016-0.3674-0.6128-1.55520.18780.34270.29792.5347-0.4519-0.14871.39490.39472.001759.543238.046647.7482
23-1.47211.1586-0.78283.42152.31750.0707-0.0233-0.05940.00060.6746-0.2149-0.04250.0603-0.31540.15492.0109-0.0415-0.02321.32460.04211.307641.702957.267171.2689
24-1.19420.25811.21970.8138-0.8-0.1738-0.3861-0.35530.34560.3867-0.02180.412-0.1044-0.28310.28751.3218-0.0168-0.2731.2561-0.12551.993821.446172.344866.3122
250.87660.03940.53920.6591-0.58072.9930.1265-0.24270.428-0.3605-0.04720.2876-1.46811.46810.18141.6106-0.46590.29651.952-0.38971.9093-23.252313.388340.6566
262.6356-1.03280.6840.36280.17962.0626-0.11380.8958-0.2607-0.20710.2957-0.0842-0.88170.1837-0.14972.18990.05160.28951.8031-0.14341.2853-56.85397.11148.0372
271.87820.2232-1.49311.1477-1.91423.47890.26671.5301-0.2124-0.6642-0.37060.3760.3167-2.56080.05291.47840.1277-0.22643.1198-0.5261.7681-100.9516-4.275321.7968
281.19420.61940.34021.183-0.21161.9268-0.0168-0.1514-1.16420.07610.3776-0.2712-0.8829-0.9238-0.02511.3520.21610.0892.13020.14362.1367-112.1593-4.834767.4553
292.7102-0.04491.26271.55670.65892.81690.0885-0.4972-0.00980.0426-0.0878-0.06390.2033-0.95110.0271.61960.07260.08781.3079-0.0351.356-78.31632.849299.7299
302.5419-0.41010.31992.26980.74112.22680.6628-0.39220.0707-0.2532-0.237-0.1088-0.52050.268-0.06511.4939-0.2173-0.10521.5986-0.1761.503-33.819312.607786.7834
311.10690.8431-1.85651.9723-0.82942.53830.29860.3940.0284-0.5171-0.36730.3948-0.2552-1.16040.01571.45770.2593-0.58181.9924-0.12931.7034-7.566342.764817.6483
322.32250.04370.6081.97091.70231.64670.58590.4219-0.3975-0.5272-0.4964-0.06840.0842-0.34560.01442.0162-0.0216-0.1161.49320.04351.276631.126424.8228-2.8086
332.8912-1.79110.08471.1665-0.09693.14470.66930.6620.1921-0.2267-0.6747-0.20630.54851.0127-0.00681.46540.23820.36051.68130.14761.263166.08878.17724.3409
343.00150.4372-1.65412.361-0.39373.51750.0008-0.1486-0.15420.0650.03640.17190.52530.55470.01521.32430.36020.06131.44750.1731.426460.56235.945571.1873
354.1326-1.18510.90952.85540.8454.1041-0.5348-0.7089-0.29810.57140.25370.24370.17620.14290.27831.39840.0910.19491.22360.11971.221221.671423.938991.2977
362.4589-1.64031.951.1887-1.2691.4256-1.0155-0.02180.2901-0.07841.0464-0.1907-0.7728-1.03950.2841.17680.3403-0.12131.7235-0.17741.7402-13.402840.752664.5154
370.9804-0.30751.74770.0796-0.54353.14010.27871.32241.16560.6571-0.82270.7774-0.09021.9479-0.45113.30750.29411.5521.2467-0.95232.2171-115.1835-28.962162.973
Refinement TLS group
IDRefine-IDRefine TLS-IDSelection detailsAuth asym-IDAuth seq-ID
1X-RAY DIFFRACTION1chain A and (resseq 1:96 or resseq 251:333 or resseq 450)A1 - 96
2X-RAY DIFFRACTION1chain A and (resseq 1:96 or resseq 251:333 or resseq 450)A251 - 333
3X-RAY DIFFRACTION1chain A and (resseq 1:96 or resseq 251:333 or resseq 450)A450
4X-RAY DIFFRACTION2chain B and (resseq 1:96 or resseq 251:333 or resseq 450)B1 - 96
5X-RAY DIFFRACTION2chain B and (resseq 1:96 or resseq 251:333 or resseq 450)B251 - 333
6X-RAY DIFFRACTION2chain B and (resseq 1:96 or resseq 251:333 or resseq 450)B450
7X-RAY DIFFRACTION3chain C and (resseq 1:96 or resseq 251:333 or resseq 450)C1 - 96
8X-RAY DIFFRACTION3chain C and (resseq 1:96 or resseq 251:333 or resseq 450)C251 - 333
9X-RAY DIFFRACTION3chain C and (resseq 1:96 or resseq 251:333 or resseq 450)C450
10X-RAY DIFFRACTION4chain D and (resseq 1:96 or resseq 251:333 or resseq 450)D1 - 96
11X-RAY DIFFRACTION4chain D and (resseq 1:96 or resseq 251:333 or resseq 450)D251 - 333
12X-RAY DIFFRACTION4chain D and (resseq 1:96 or resseq 251:333 or resseq 450)D450
13X-RAY DIFFRACTION5chain E and (resseq 1:96 or resseq 251:333 or resseq 450)E1 - 96
14X-RAY DIFFRACTION5chain E and (resseq 1:96 or resseq 251:333 or resseq 450)E251 - 333
15X-RAY DIFFRACTION5chain E and (resseq 1:96 or resseq 251:333 or resseq 450)E450
16X-RAY DIFFRACTION6chain F and (resseq 1:96 or resseq 251:333 or resseq 450)F1 - 96
17X-RAY DIFFRACTION6chain F and (resseq 1:96 or resseq 251:333 or resseq 450)F251 - 333
18X-RAY DIFFRACTION6chain F and (resseq 1:96 or resseq 251:333 or resseq 450)F450
19X-RAY DIFFRACTION7chain G and (resseq 1:96 or resseq 251:333 or resseq 450)G1 - 96
20X-RAY DIFFRACTION7chain G and (resseq 1:96 or resseq 251:333 or resseq 450)G251 - 333
21X-RAY DIFFRACTION7chain G and (resseq 1:96 or resseq 251:333 or resseq 450)G450
22X-RAY DIFFRACTION8chain H and (resseq 1:96 or resseq 251:333 or resseq 450)H1 - 96
23X-RAY DIFFRACTION8chain H and (resseq 1:96 or resseq 251:333 or resseq 450)H251 - 333
24X-RAY DIFFRACTION8chain H and (resseq 1:96 or resseq 251:333 or resseq 450)H450
25X-RAY DIFFRACTION9chain I and (resseq 1:96 or resseq 251:333 or resseq 450)I1 - 96
26X-RAY DIFFRACTION9chain I and (resseq 1:96 or resseq 251:333 or resseq 450)I251 - 333
27X-RAY DIFFRACTION9chain I and (resseq 1:96 or resseq 251:333 or resseq 450)I450
28X-RAY DIFFRACTION10chain J and (resseq 1:96 or resseq 251:333 or resseq 450)J1 - 96
29X-RAY DIFFRACTION10chain J and (resseq 1:96 or resseq 251:333 or resseq 450)J251 - 333
30X-RAY DIFFRACTION10chain J and (resseq 1:96 or resseq 251:333 or resseq 450)J450
31X-RAY DIFFRACTION11chain K and (resseq 1:96 or resseq 251:333 or resseq 450)K1 - 96
32X-RAY DIFFRACTION11chain K and (resseq 1:96 or resseq 251:333 or resseq 450)K251 - 333
33X-RAY DIFFRACTION11chain K and (resseq 1:96 or resseq 251:333 or resseq 450)K450
34X-RAY DIFFRACTION12chain L and (resseq 1:96 or resseq 251:333 or resseq 450)L1 - 96
35X-RAY DIFFRACTION12chain L and (resseq 1:96 or resseq 251:333 or resseq 450)L251 - 333
36X-RAY DIFFRACTION12chain L and (resseq 1:96 or resseq 251:333 or resseq 450)L450
37X-RAY DIFFRACTION13chain A and (resseq 97:250)A97 - 250
38X-RAY DIFFRACTION14chain B and (resseq 97:250)B97 - 250
39X-RAY DIFFRACTION15chain C and (resseq 97:250)C97 - 250
40X-RAY DIFFRACTION16chain D and (resseq 97:250)D97 - 250
41X-RAY DIFFRACTION17chain E and (resseq 97:250)E97 - 250
42X-RAY DIFFRACTION18chain F and (resseq 97:250)F97 - 250
43X-RAY DIFFRACTION19chain G and (resseq 97:250)G97 - 250
44X-RAY DIFFRACTION20chain H and (resseq 97:250)H97 - 250
45X-RAY DIFFRACTION21chain I and (resseq 97:250)I97 - 250
46X-RAY DIFFRACTION22chain J and (resseq 97:250)J97 - 250
47X-RAY DIFFRACTION23chain K and (resseq 97:250)K97 - 250
48X-RAY DIFFRACTION24chain L and (resseq 97:250)L97 - 250
49X-RAY DIFFRACTION25chain A and (resseq 334:443)A334 - 443
50X-RAY DIFFRACTION26chain B and (resseq 334:443)B334 - 443
51X-RAY DIFFRACTION27chain C and (resseq 334:443)C334 - 443
52X-RAY DIFFRACTION28chain D and (resseq 334:443)D334 - 443
53X-RAY DIFFRACTION29chain E and (resseq 334:443)E334 - 443
54X-RAY DIFFRACTION30chain F and (resseq 334:443)F334 - 443
55X-RAY DIFFRACTION31chain G and (resseq 334:443)G334 - 443
56X-RAY DIFFRACTION32chain H and (resseq 334:443)H334 - 443
57X-RAY DIFFRACTION33chain I and (resseq 334:443)I334 - 443
58X-RAY DIFFRACTION34chain J and (resseq 334:443)J334 - 443
59X-RAY DIFFRACTION35chain K and (resseq 334:443)K334 - 443
60X-RAY DIFFRACTION36chain L and (resseq 334:443)L334 - 443
61X-RAY DIFFRACTION37chain XX3 - 18

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