+Open data
-Basic information
Entry | Database: PDB / ID: 6prg | |||||||||
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Title | SBP RafE in complex with stachyose | |||||||||
Components | ABC transporter sugar-binding protein | |||||||||
Keywords | SUGAR BINDING PROTEIN / Cluster D-I solute binding protein / complex / SBP | |||||||||
Function / homology | Function and homology information | |||||||||
Biological species | Streptococcus pneumoniae TIGR4 (bacteria) | |||||||||
Method | X-RAY DIFFRACTION / MOLECULAR REPLACEMENT / molecular replacement / Resolution: 2.35 Å | |||||||||
Authors | Meier, E.P.W. / Boraston, A.B. | |||||||||
Funding support | Canada, 1items
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Citation | Journal: J.Biol.Chem. / Year: 2019 Title: Molecular analysis of an enigmaticStreptococcus pneumoniaevirulence factor: The raffinose-family oligosaccharide utilization system. Authors: Hobbs, J.K. / Meier, E.P.W. / Pluvinage, B. / Mey, M.A. / Boraston, A.B. | |||||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 6prg.cif.gz | 171.6 KB | Display | PDBx/mmCIF format |
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PDB format | pdb6prg.ent.gz | 130.9 KB | Display | PDB format |
PDBx/mmJSON format | 6prg.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/pr/6prg ftp://data.pdbj.org/pub/pdb/validation_reports/pr/6prg | HTTPS FTP |
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-Related structure data
Related structure data | 6phuC 6phvC 6phwC 6phxC 6phyC 6pi0C 6pqlC 6preC 2i58S S: Starting model for refinement C: citing same article (ref.) |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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1 |
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2 |
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Unit cell |
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-Components
#1: Protein | Mass: 46440.012 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Streptococcus pneumoniae TIGR4 (bacteria) Gene: SP_1897 / Plasmid: pET28a / Production host: Escherichia coli BL21(DE3) (bacteria) / References: UniProt: A0A0E8ZWG6, UniProt: A0A0H2URJ7*PLUS #2: Polysaccharide | Source method: isolated from a genetically manipulated source #3: Chemical | #4: Chemical | ChemComp-EDO / #5: Water | ChemComp-HOH / | Has ligand of interest | Y | |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 2.3 Å3/Da / Density % sol: 46.56 % |
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Crystal grow | Temperature: 291 K / Method: vapor diffusion, sitting drop / pH: 8 / Details: 0.2M CsCl, 0.1M Tris, 20% w/v PEG 3350 |
-Data collection
Diffraction | Mean temperature: 100 K / Serial crystal experiment: N |
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Diffraction source | Source: ROTATING ANODE / Type: RIGAKU MICROMAX-002 / Wavelength: 1.54187 Å |
Detector | Type: DECTRIS PILATUS 200K / Detector: PIXEL / Date: Jan 21, 2017 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.54187 Å / Relative weight: 1 |
Reflection | Resolution: 2.35→30 Å / Num. obs: 36605 / % possible obs: 99.9 % / Redundancy: 5 % / CC1/2: 0.995 / Rmerge(I) obs: 0.084 / Rpim(I) all: 0.036 / Net I/σ(I): 20.2 |
Reflection shell | Resolution: 2.35→2.39 Å / Rmerge(I) obs: 0.181 / Num. unique obs: 3742 / CC1/2: 0.966 / Rpim(I) all: 0.095 |
-Phasing
Phasing | Method: molecular replacement |
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-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: 2i58 Resolution: 2.35→29.94 Å / Cor.coef. Fo:Fc: 0.927 / Cor.coef. Fo:Fc free: 0.909 / SU B: 7.108 / SU ML: 0.168 / Cross valid method: THROUGHOUT / σ(F): 0 / ESU R: 0.466 / ESU R Free: 0.257 / Details: U VALUES : REFINED INDIVIDUALLY
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Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å | |||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso max: 54.66 Å2 / Biso mean: 28.74 Å2 / Biso min: 17.79 Å2
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Refinement step | Cycle: final / Resolution: 2.35→29.94 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 2.35→2.411 Å / Rfactor Rfree error: 0 / Total num. of bins used: 20
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