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Open data
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Basic information
Entry | Database: PDB / ID: 6p57 | |||||||||
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Title | Crystal Structure of the Beta Subunit of Luteinizing Hormone | |||||||||
![]() | Lutropin subunit beta | |||||||||
![]() | HORMONE / reproduction / glycosylation / gonadotropic hormone / cysteine knot | |||||||||
Function / homology | ![]() Mineralocorticoid biosynthesis / Glycoprotein hormones / Hormone ligand-binding receptors / TFAP2 (AP-2) family regulates transcription of growth factors and their receptors / Reactions specific to the complex N-glycan synthesis pathway / hormone-mediated signaling pathway / hormone activity / G protein-coupled receptor signaling pathway / extracellular space / cytoplasm Similarity search - Function | |||||||||
Biological species | ![]() ![]() | |||||||||
Method | ![]() ![]() ![]() | |||||||||
![]() | McPherson, A. | |||||||||
![]() | ![]() Title: The Crystal Structure of the Beta Subunit of Luteinizing Hormone and a Model for the Intact Hormone Authors: Larson, S.B. / McPherson, A. | |||||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 111.7 KB | Display | ![]() |
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PDB format | ![]() | 85.6 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 1.5 MB | Display | ![]() |
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Full document | ![]() | 1.5 MB | Display | |
Data in XML | ![]() | 15.2 KB | Display | |
Data in CIF | ![]() | 19.4 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 1hcnS S: Starting model for refinement |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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1 | ![]()
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2 | ![]()
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Unit cell |
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Components
-Protein / Non-polymers , 2 types, 25 molecules AB![](data/chem/img/HOH.gif)
![](data/chem/img/HOH.gif)
#1: Protein | Mass: 15215.161 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) ![]() ![]() #6: Water | ChemComp-HOH / | |
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-Sugars , 4 types, 5 molecules ![](data/chem/img/MAN.gif)
![](data/chem/img/BOG.gif)
![](data/chem/img/BOG.gif)
#2: Polysaccharide | beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-[beta-L-fucopyranose-(1-6) ...beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-[beta-L-fucopyranose-(1-6)]2-acetamido-2-deoxy-beta-D-glucopyranose Source method: isolated from a genetically manipulated source |
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#3: Polysaccharide | 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-2)-alpha-D-mannopyranose-(1-6)-beta-D-mannopyranose-(1- ...2-acetamido-2-deoxy-beta-D-glucopyranose-(1-2)-alpha-D-mannopyranose-(1-6)-beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-[beta-L-fucopyranose-(1-6)]2-acetamido-2-deoxy-beta-D-glucopyranose Source method: isolated from a genetically manipulated source |
#4: Sugar | ChemComp-MAN / |
#5: Sugar |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.79 Å3/Da / Density % sol: 56 % / Description: thin plates |
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Crystal grow | Temperature: 298 K / Method: vapor diffusion, sitting drop / pH: 6.5 Details: Sitting drop with the drop composed of equal quantities of 20 mg/ml protein dissolved in water, and 20% PEG 3350 plus 1% beta octyl glucoside dissolved in 0.10 M MES buffer at pH 6.5. Room ...Details: Sitting drop with the drop composed of equal quantities of 20 mg/ml protein dissolved in water, and 20% PEG 3350 plus 1% beta octyl glucoside dissolved in 0.10 M MES buffer at pH 6.5. Room temperature crystallization. PH range: 6.0 - 7.5 |
-Data collection
Diffraction | Mean temperature: 298 K / Serial crystal experiment: N |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: MARRESEARCH / Detector: CCD / Date: Jun 12, 1998 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.1 Å / Relative weight: 1 |
Reflection | Resolution: 3→60 Å / Num. obs: 6465 / % possible obs: 98.43 % / Redundancy: 9.35 % / Biso Wilson estimate: 85 Å2 / CC1/2: 0.89 / Rmerge(I) obs: 0.15 / Rrim(I) all: 0.16 / Rsym value: 0.14 / Net I/σ(I): 9.1 |
Reflection shell | Resolution: 3→3.15 Å / Redundancy: 9.6 % / Rmerge(I) obs: 0.64 / Mean I/σ(I) obs: 3.3 / Num. unique obs: 480 / CC1/2: 0.34 / Rrim(I) all: 0.68 / Rsym value: 0.6 / % possible all: 98 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: 1HCN Resolution: 3.16→33.32 Å / Cor.coef. Fo:Fc: 0.939 / Cor.coef. Fo:Fc free: 0.83 / SU B: 11.107 / SU ML: 0.143 / Cross valid method: THROUGHOUT / ESU R Free: 0.114 / Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS
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Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 146.762 Å2
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Refinement step | Cycle: 1 / Resolution: 3.16→33.32 Å
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Refine LS restraints |
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