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- PDB-6owk: Crystal structure of a Bacillus thuringiensis Cry1B.867 tryptic c... -
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Open data
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Basic information
Entry | Database: PDB / ID: 6owk | ||||||
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Title | Crystal structure of a Bacillus thuringiensis Cry1B.867 tryptic core variant | ||||||
![]() | Pesticidal crystal protein Cry1Be, Cry1K-like protein chimera | ||||||
![]() | TOXIN / Bacillus thuringiensis / Cry toxin / B.t. | ||||||
Function / homology | ![]() sporulation resulting in formation of a cellular spore / : / toxin activity / signaling receptor binding Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Rydel, T.J. / Evdokimov, A. / Beishir, S.C. | ||||||
![]() | ![]() Title: Bacillus thuringiensis Cry1Da_7 and Cry1B.868 Protein Interactions with Novel Receptors Allow Control of Resistant Fall Armyworms, Spodoptera frugiperda (J.E. Smith). Authors: Wang, Y. / Wang, J. / Fu, X. / Nageotte, J.R. / Silverman, J. / Bretsnyder, E.C. / Chen, D. / Rydel, T.J. / Bean, G.J. / Li, K.S. / Kraft, E. / Gowda, A. / Nance, A. / Moore, R.G. / Pleau, M. ...Authors: Wang, Y. / Wang, J. / Fu, X. / Nageotte, J.R. / Silverman, J. / Bretsnyder, E.C. / Chen, D. / Rydel, T.J. / Bean, G.J. / Li, K.S. / Kraft, E. / Gowda, A. / Nance, A. / Moore, R.G. / Pleau, M.J. / Milligan, J.S. / Anderson, H.M. / Asiimwe, P. / Evans, A. / Moar, W.J. / Martinelli, S. / Head, G.P. / Haas, J.A. / Baum, J.A. / Yang, F. / Kerns, D.L. / Jerga, A. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 129.4 KB | Display | ![]() |
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PDB format | ![]() | 98.3 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 424.2 KB | Display | ![]() |
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Full document | ![]() | 437.8 KB | Display | |
Data in XML | ![]() | 22.4 KB | Display | |
Data in CIF | ![]() | 29.6 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 6ovbC ![]() 3eb7S S: Starting model for refinement C: citing same article ( |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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1 | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 69714.469 Da / Num. of mol.: 1 Fragment: Cry1Be (UNP residues 35-510) + Cry1K (UNP residues 481-621) Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() References: UniProt: O85805, UniProt: V9I1B1, UniProt: Q45715*PLUS |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.06 Å3/Da / Density % sol: 40 % / Description: Star of David-shaped, six-sided crystals |
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Crystal grow | Temperature: 298 K / Method: vapor diffusion, sitting drop / pH: 10.3 / Details: 20% PEG3350, 0.2 M ammonium chloride |
-Data collection
Diffraction | Mean temperature: 100 K / Serial crystal experiment: N |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: MARRESEARCH / Detector: CCD / Date: Dec 6, 2013 |
Radiation | Monochromator: double crystal Si(111) / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1 Å / Relative weight: 1 |
Reflection | Resolution: 2.698→38.691 Å / Num. obs: 15172 / % possible obs: 93.5 % / Redundancy: 4.3 % / Biso Wilson estimate: 43.6 Å2 / Rmerge(I) obs: 0.11 / Net I/σ(I): 25.2 |
Reflection shell | Resolution: 2.7→2.75 Å / Redundancy: 2.3 % / Rmerge(I) obs: 0.277 / Mean I/σ(I) obs: 4.83 / Num. unique obs: 597 / % possible all: 79.6 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: PDB entry 3EB7 Resolution: 2.698→38.691 Å / SU ML: 0.31 / Cross valid method: FREE R-VALUE / σ(F): 1.38 / Phase error: 32.97
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2.698→38.691 Å
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Refine LS restraints |
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LS refinement shell |
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