Mass: 18.015 Da / Num. of mol.: 69 / Source method: isolated from a natural source / Formula: H2O
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Experimental details
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Experiment
Experiment
Method: X-RAY DIFFRACTION / Number of used crystals: 1
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Sample preparation
Crystal
Density Matthews: 3.06 Å3/Da / Density % sol: 60 %
Crystal grow
Temperature: 290 K / Method: vapor diffusion, sitting drop / pH: 7.5 Details: Optimization screen based on JCSG B12: 16.82% (w/V) PEG 3350, 200mM potassium citrate tribasic: LpboA.19561.b.B1.PS38498 at 15mg/ml, grown at 4C: cryo: 20% EG in 2 steps, tray 307870 b3: ...Details: Optimization screen based on JCSG B12: 16.82% (w/V) PEG 3350, 200mM potassium citrate tribasic: LpboA.19561.b.B1.PS38498 at 15mg/ml, grown at 4C: cryo: 20% EG in 2 steps, tray 307870 b3: puck rvo6-2. For experimental phasing, a crystal from the same optimization screen, well C10, with LpboA.19561.b.B1.PS38498 at 22mg/ml, grown at 14C was incubated for 15 sec each in a solution of 90% reservoir and 10% 2.5M sodium ioidide in ethylene glycol, and a solution of 80% reservoir and 20% 2.5M sodium iodide in ethylene glycol, and vitrified in liquid nitrogen: tray 305816 C10: puck vvv2-11
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