Yeast Spc42 N-terminal coiled-coil fused to PDB: 3K2N
Components
Spindle pole body component SPC42,Sigma-54-dependent transcriptional regulator
Keywords
STRUCTURAL PROTEIN / Yeast Centrosome / Coiled-Coil / Spindle Pole Body
Function / homology
Function and homology information
intermediate layer of spindle pole body / central plaque of spindle pole body / spindle pole body duplication / regulation of microtubule nucleation / structural constituent of cytoskeleton / sequence-specific DNA binding / regulation of DNA-templated transcription / ATP hydrolysis activity / ATP binding / nucleus / cytoplasm Similarity search - Function
Spindle pole body component Spc42 / Spindle pole body component Spc42p / Sigma-54 interaction domain ATP-binding region A signature. / Sigma-54 interaction domain, ATP-binding site 2 / Sigma-54 interaction domain ATP-binding region B signature. / Sigma-54 interaction domain, ATP-binding site 1 / Sigma-54 interaction domain profile. / Sigma-54 interaction domain / RNA polymerase sigma factor 54 interaction domain / DNA binding HTH domain, Fis-type ...Spindle pole body component Spc42 / Spindle pole body component Spc42p / Sigma-54 interaction domain ATP-binding region A signature. / Sigma-54 interaction domain, ATP-binding site 2 / Sigma-54 interaction domain ATP-binding region B signature. / Sigma-54 interaction domain, ATP-binding site 1 / Sigma-54 interaction domain profile. / Sigma-54 interaction domain / RNA polymerase sigma factor 54 interaction domain / DNA binding HTH domain, Fis-type / Bacterial regulatory protein, Fis family / GAF domain / Domain present in phytochromes and cGMP-specific phosphodiesterases. / GAF domain / GAF-like domain superfamily / Homeobox-like domain superfamily / ATPases associated with a variety of cellular activities / AAA+ ATPase domain / P-loop containing nucleoside triphosphate hydrolase Similarity search - Domain/homology
Mass: 18.015 Da / Num. of mol.: 41 / Source method: isolated from a natural source / Formula: H2O
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Experimental details
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Experiment
Experiment
Method: X-RAY DIFFRACTION / Number of used crystals: 1
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Sample preparation
Crystal
Density Matthews: 3.36 Å3/Da / Density % sol: 63.44 %
Crystal grow
Temperature: 298 K / Method: vapor diffusion, hanging drop / pH: 7 Details: Crystals were grown from a 1:1 mixture of 16 mg/ml protein solution containing 20 mM HEPES pH7.6, 100 mM NaCl and well solution containing 100 mM MOPS pH 7.0, 5% (w/v) PEG4K, 5%(w/v) MPD, and 150 mM NaCitrate.
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Data collection
Diffraction
Mean temperature: 100 K / Serial crystal experiment: N
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