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- PDB-6obg: Ricin A chain bound to VHH antibody V8E6 -

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Basic information

Entry
Database: PDB / ID: 6obg
TitleRicin A chain bound to VHH antibody V8E6
Components
  • Ricin A chain
  • VHH antibody V8E6
KeywordsTOXIN
Function / homology
Function and homology information


rRNA N-glycosylase / rRNA N-glycosylase activity / AMP binding / defense response / toxin activity / carbohydrate binding / killing of cells of another organism / negative regulation of translation
Similarity search - Function
Ricin (A Subunit), domain 2 / Ricin (A Subunit), domain 2 / Ricin (A subunit); domain 1 / Ricin (A subunit), domain 1 / Ricin-type beta-trefoil lectin domain / Ribosome-inactivating protein conserved site / Shiga/ricin ribosomal inactivating toxins active site signature. / Ribosome-inactivating protein type 1/2 / Ribosome-inactivating protein / Ribosome-inactivating protein, subdomain 1 ...Ricin (A Subunit), domain 2 / Ricin (A Subunit), domain 2 / Ricin (A subunit); domain 1 / Ricin (A subunit), domain 1 / Ricin-type beta-trefoil lectin domain / Ribosome-inactivating protein conserved site / Shiga/ricin ribosomal inactivating toxins active site signature. / Ribosome-inactivating protein type 1/2 / Ribosome-inactivating protein / Ribosome-inactivating protein, subdomain 1 / Ribosome-inactivating protein, subdomain 2 / Ribosome-inactivating protein superfamily / Ribosome inactivating protein / Ricin-type beta-trefoil / Lectin domain of ricin B chain profile. / Ricin B, lectin domain / Ricin B-like lectins / Few Secondary Structures / Irregular / 3-Layer(aba) Sandwich / Alpha Beta
Similarity search - Domain/homology
Biological speciesRicinus communis (castor bean)
Vicugna pacos (alpaca)
MethodX-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 1.996 Å
AuthorsRudolph, M.J.
Funding support United States, 1items
OrganizationGrant numberCountry
National Institutes of Health/National Institute Of Allergy and Infectious Diseases (NIH/NIAID) United States
CitationJournal: J.Mol.Biol. / Year: 2020
Title: Intracellular Neutralization of Ricin Toxin by Single-domain Antibodies Targeting the Active Site.
Authors: Rudolph, M.J. / Czajka, T.F. / Davis, S.A. / Thi Nguyen, C.M. / Li, X.P. / Tumer, N.E. / Vance, D.J. / Mantis, N.J.
History
DepositionMar 20, 2019Deposition site: RCSB / Processing site: RCSB
Revision 1.0Apr 1, 2020Provider: repository / Type: Initial release
Revision 1.1Oct 14, 2020Group: Database references / Derived calculations
Category: citation / citation_author ...citation / citation_author / pdbx_struct_conn_angle / struct_conn
Item: _citation.country / _citation.journal_abbrev ..._citation.country / _citation.journal_abbrev / _citation.journal_id_ASTM / _citation.journal_id_CSD / _citation.journal_id_ISSN / _citation.journal_volume / _citation.page_first / _citation.page_last / _citation.pdbx_database_id_DOI / _citation.pdbx_database_id_PubMed / _citation.title / _citation.year / _pdbx_struct_conn_angle.ptnr1_auth_asym_id / _pdbx_struct_conn_angle.ptnr1_auth_comp_id / _pdbx_struct_conn_angle.ptnr1_auth_seq_id / _pdbx_struct_conn_angle.ptnr1_label_asym_id / _pdbx_struct_conn_angle.ptnr1_label_atom_id / _pdbx_struct_conn_angle.ptnr1_label_comp_id / _pdbx_struct_conn_angle.ptnr1_label_seq_id / _pdbx_struct_conn_angle.ptnr1_symmetry / _pdbx_struct_conn_angle.ptnr2_auth_asym_id / _pdbx_struct_conn_angle.ptnr2_auth_seq_id / _pdbx_struct_conn_angle.ptnr2_label_asym_id / _pdbx_struct_conn_angle.ptnr2_symmetry / _pdbx_struct_conn_angle.ptnr3_auth_asym_id / _pdbx_struct_conn_angle.ptnr3_auth_comp_id / _pdbx_struct_conn_angle.ptnr3_auth_seq_id / _pdbx_struct_conn_angle.ptnr3_label_asym_id / _pdbx_struct_conn_angle.ptnr3_label_atom_id / _pdbx_struct_conn_angle.ptnr3_label_comp_id / _pdbx_struct_conn_angle.ptnr3_label_seq_id / _pdbx_struct_conn_angle.ptnr3_symmetry / _pdbx_struct_conn_angle.value / _struct_conn.pdbx_dist_value / _struct_conn.ptnr1_auth_asym_id / _struct_conn.ptnr1_auth_comp_id / _struct_conn.ptnr1_auth_seq_id / _struct_conn.ptnr1_label_asym_id / _struct_conn.ptnr1_label_atom_id / _struct_conn.ptnr1_label_comp_id / _struct_conn.ptnr1_label_seq_id / _struct_conn.ptnr1_symmetry / _struct_conn.ptnr2_auth_asym_id / _struct_conn.ptnr2_auth_comp_id / _struct_conn.ptnr2_auth_seq_id / _struct_conn.ptnr2_label_asym_id / _struct_conn.ptnr2_label_atom_id / _struct_conn.ptnr2_label_comp_id / _struct_conn.ptnr2_label_seq_id / _struct_conn.ptnr2_symmetry
Revision 1.2Oct 30, 2024Group: Data collection / Database references ...Data collection / Database references / Refinement description / Structure summary
Category: chem_comp_atom / chem_comp_bond ...chem_comp_atom / chem_comp_bond / database_2 / pdbx_entry_details / pdbx_modification_feature / struct_ncs_dom_lim
Item: _database_2.pdbx_DOI / _database_2.pdbx_database_accession ..._database_2.pdbx_DOI / _database_2.pdbx_database_accession / _struct_ncs_dom_lim.beg_auth_comp_id / _struct_ncs_dom_lim.beg_label_asym_id / _struct_ncs_dom_lim.beg_label_comp_id / _struct_ncs_dom_lim.beg_label_seq_id / _struct_ncs_dom_lim.end_auth_comp_id / _struct_ncs_dom_lim.end_label_asym_id / _struct_ncs_dom_lim.end_label_comp_id / _struct_ncs_dom_lim.end_label_seq_id

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Assembly

Deposited unit
A: Ricin A chain
B: Ricin A chain
C: VHH antibody V8E6
D: VHH antibody V8E6
hetero molecules


Theoretical massNumber of molelcules
Total (without water)83,83616
Polymers83,1414
Non-polymers69512
Water5,837324
1
A: Ricin A chain
C: VHH antibody V8E6
hetero molecules


Theoretical massNumber of molelcules
Total (without water)41,9689
Polymers41,5712
Non-polymers3987
Water362
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area3010 Å2
ΔGint-129 kcal/mol
Surface area15640 Å2
MethodPISA
2
B: Ricin A chain
D: VHH antibody V8E6
hetero molecules


Theoretical massNumber of molelcules
Total (without water)41,8687
Polymers41,5712
Non-polymers2975
Water362
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area2670 Å2
ΔGint-82 kcal/mol
Surface area15650 Å2
MethodPISA
Unit cell
Length a, b, c (Å)59.795, 77.885, 92.597
Angle α, β, γ (deg.)90.000, 98.950, 90.000
Int Tables number4
Space group name H-MP1211
Noncrystallographic symmetry (NCS)NCS domain:
IDEns-IDDetails
11(chain A and (resid 5 through 79 or resid 81 through 261))
21(chain B and (resid 5 through 79 or resid 81 through 261))
12(chain C and (resid 3 through 41 or resid 44 through 119))
22(chain D and (resid 3 through 41 or resid 44 through 119))

NCS domain segments:
Dom-IDComponent-IDEns-IDBeg auth comp-IDBeg label comp-IDEnd auth comp-IDEnd label comp-IDSelection detailsAuth asym-IDLabel asym-IDAuth seq-IDLabel seq-ID
111GLNGLNALAALA(chain A and (resid 5 through 79 or resid 81 through 261))AA5 - 791 - 75
121VALVALPROPRO(chain A and (resid 5 through 79 or resid 81 through 261))AA81 - 26177 - 257
211GLNGLNALAALA(chain B and (resid 5 through 79 or resid 81 through 261))BB5 - 791 - 75
221VALVALPROPRO(chain B and (resid 5 through 79 or resid 81 through 261))BB81 - 26177 - 257
112GLNGLNPROPRO(chain C and (resid 3 through 41 or resid 44 through 119))CC3 - 411 - 39
122GLUGLUSERSER(chain C and (resid 3 through 41 or resid 44 through 119))CC44 - 11942 - 117
212GLNGLNPROPRO(chain D and (resid 3 through 41 or resid 44 through 119))DD3 - 411 - 39
222GLUGLUSERSER(chain D and (resid 3 through 41 or resid 44 through 119))DD44 - 11942 - 117

NCS ensembles :
ID
1
2

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Components

#1: Protein Ricin A chain


Mass: 28806.449 Da / Num. of mol.: 2 / Fragment: Toxin catalytic subunit, residues 40-296
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Ricinus communis (castor bean) / Production host: Escherichia coli (E. coli) / References: UniProt: P02879, rRNA N-glycosylase
#2: Antibody VHH antibody V8E6


Mass: 12764.086 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Vicugna pacos (alpaca) / Production host: Escherichia coli (E. coli)
#3: Chemical
ChemComp-ZN / ZINC ION


Mass: 65.409 Da / Num. of mol.: 9 / Source method: obtained synthetically / Formula: Zn
#4: Chemical ChemComp-CL / CHLORIDE ION


Mass: 35.453 Da / Num. of mol.: 3 / Source method: obtained synthetically / Formula: Cl
#5: Water ChemComp-HOH / water


Mass: 18.015 Da / Num. of mol.: 324 / Source method: isolated from a natural source / Formula: H2O
Has protein modificationY

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Experimental details

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Experiment

ExperimentMethod: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

CrystalDensity Matthews: 2.56 Å3/Da / Density % sol: 51.99 %
Crystal growTemperature: 295 K / Method: vapor diffusion / pH: 6
Details: 100 mM MES (pH 6.0), 170 mM zinc acetate, 6% PEG 8,000, and 0.5% ethyl acetate

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Data collection

DiffractionMean temperature: 100 K / Serial crystal experiment: N
Diffraction sourceSource: SYNCHROTRON / Site: APS / Beamline: 24-ID-C / Wavelength: 0.979 Å
DetectorType: DECTRIS PILATUS 6M-F / Detector: PIXEL / Date: Mar 17, 2017
RadiationProtocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelengthWavelength: 0.979 Å / Relative weight: 1
ReflectionResolution: 1.996→50 Å / Num. obs: 54931 / % possible obs: 96.2 % / Redundancy: 3.2 % / Rmerge(I) obs: 0.081 / Rpim(I) all: 0.052 / Rrim(I) all: 0.097 / Χ2: 0.891 / Net I/σ(I): 5.3
Reflection shell

Diffraction-ID: 1

Resolution (Å)Redundancy (%)Rmerge(I) obsNum. unique obsCC1/2Rpim(I) allRrim(I) allΧ2% possible all
2-2.032.90.7125700.6870.4870.8650.45191.8
2.03-2.072.80.61426150.7480.420.7470.4691.9
2.07-2.113.10.49127350.8410.320.5880.46495.9
2.11-2.153.30.4327200.8730.2750.5120.47896.3
2.15-2.23.20.35127560.9070.2260.4190.49596.9
2.2-2.253.20.30427470.9150.1960.3630.52296.6
2.25-2.313.20.2627780.9430.1680.310.51497.2
2.31-2.373.20.2327580.9480.150.2760.53497.2
2.37-2.443.10.21327320.9560.1410.2560.56396.6
2.44-2.522.90.19726920.9590.1330.2390.56993.5
2.52-2.613.30.14727810.980.0940.1750.698.1
2.61-2.713.30.1328010.9810.0820.1540.68198.1
2.71-2.843.30.11327940.9830.0720.1340.72298
2.84-2.993.30.0928040.9880.0570.1070.78198
2.99-3.173.20.08127600.9880.0540.0970.9696.8
3.17-3.423.20.07727060.990.0490.0921.22295
3.42-3.763.40.07128320.990.0440.0841.50298.3
3.76-4.313.30.06528050.9930.0410.0771.78597.4
4.31-5.433.20.06127290.9930.0390.0731.98494.3
5.43-503.30.05728160.9950.0360.0672.11995.7

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Processing

Software
NameVersionClassification
SCALEPACKdata scaling
PHENIX1.11.1_2575refinement
PDB_EXTRACT3.24data extraction
HKL-2000data reduction
PHASERphasing
RefinementMethod to determine structure: MOLECULAR REPLACEMENT / Resolution: 1.996→45.735 Å / SU ML: 0.23 / Cross valid method: THROUGHOUT / σ(F): 1.37 / Phase error: 28.15
RfactorNum. reflection% reflection
Rfree0.218 2655 4.84 %
Rwork0.189 --
obs0.1905 54868 95.94 %
Solvent computationShrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å
Displacement parametersBiso max: 124.41 Å2 / Biso mean: 52.7046 Å2 / Biso min: 22.11 Å2
Refinement stepCycle: final / Resolution: 1.996→45.735 Å
ProteinNucleic acidLigandSolventTotal
Num. atoms5843 0 12 324 6179
Biso mean--59.01 52.57 -
Num. residues----746
Refine LS restraints
Refine-IDTypeDev idealNumber
X-RAY DIFFRACTIONf_bond_d0.0065977
X-RAY DIFFRACTIONf_angle_d0.7978121
X-RAY DIFFRACTIONf_chiral_restr0.053896
X-RAY DIFFRACTIONf_plane_restr0.0061069
X-RAY DIFFRACTIONf_dihedral_angle_d15.3213553
Refine LS restraints NCS
Ens-IDDom-IDAuth asym-IDNumberRefine-IDRmsType
11A2429X-RAY DIFFRACTION7.95TORSIONAL
12B2429X-RAY DIFFRACTION7.95TORSIONAL
21C1045X-RAY DIFFRACTION7.95TORSIONAL
22D1045X-RAY DIFFRACTION7.95TORSIONAL
LS refinement shell

Refine-ID: X-RAY DIFFRACTION / Rfactor Rfree error: 0 / Total num. of bins used: 19

Resolution (Å)Rfactor RfreeNum. reflection RfreeRfactor RworkNum. reflection RworkNum. reflection all% reflection obs (%)
1.9955-2.03180.33141220.28582496261888
2.0318-2.07090.3221270.27172653278092
2.0709-2.11320.26891370.25792714285196
2.1132-2.15910.32291300.25172762289296
2.1591-2.20940.29761380.24092761289997
2.2094-2.26460.25181360.23472746288297
2.2646-2.32580.2921370.23572803294097
2.3258-2.39430.29471390.22522764290397
2.3943-2.47160.27241320.22612736286896
2.4716-2.55990.27331380.21152690282895
2.5599-2.66240.24321470.20162817296498
2.6624-2.78350.24661550.20372789294498
2.7835-2.93020.26161300.20232800293098
2.9302-3.11380.24051660.19662780294697
3.1138-3.35410.23351410.18922721286295
3.3541-3.69160.19771290.17022842297198
3.6916-4.22540.15951440.15232796294098
4.2254-5.32230.16861610.14882712287394
5.3223-45.74690.19071460.17952831297796
Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

IDL11 (°2)L12 (°2)L13 (°2)L22 (°2)L23 (°2)L33 (°2)S11 (Å °)S12 (Å °)S13 (Å °)S21 (Å °)S22 (Å °)S23 (Å °)S31 (Å °)S32 (Å °)S33 (Å °)T11 (Å2)T12 (Å2)T13 (Å2)T22 (Å2)T23 (Å2)T33 (Å2)Origin x (Å)Origin y (Å)Origin z (Å)
11.0549-0.9399-0.52065.9428-0.46593.77750.11010.1489-0.0027-0.1075-0.08070.3149-0.0455-0.1573-0.03560.2957-0.0428-0.0150.2521-0.00010.1904-7.66550.867533.7841
21.8514-2.69861.00084.3607-0.46154.2828-0.0155-0.02910.00510.48630.0354-0.0780.11860.13150.01770.3998-0.01810.01440.22370.00240.1818-3.1004-1.909246.9916
36.689-3.3024-1.8872.21090.80963.9971-0.0795-0.37380.10050.61790.1928-0.0244-0.39430.0551-0.06660.66720.00040.05420.3158-0.0570.2942-2.98655.498553.5295
41.60850.5502-0.51533.9559-0.48843.1183-0.0294-0.0633-0.0685-0.12380.0369-0.32920.16870.2871-0.02370.28970.02510.00330.2337-0.04070.22178.4938-4.055935.8974
57.8264-1.3404-2.98214.18491.46836.51720.48260.20670.6065-0.5559-0.1201-0.41-0.84140.2938-0.35810.5641-0.04380.07280.28480.00990.30365.74217.912723.245
63.7681-0.24-0.18749.9-0.83165.4227-0.13560.4630.3433-1.0963-0.1594-0.789-0.15540.30350.23420.5383-0.04330.10470.36710.05540.405923.4859-16.5309-7.8649
73.81070.9911.14723.41751.19951.7929-0.12120.5013-0.2711-0.39370.2197-0.74330.07980.2514-0.0610.53430.00380.1130.3273-0.01470.427423.1034-26.5367-4.1077
86.04321.09694.2354.52923.08747.1689-0.069-0.1941-0.09230.1582-0.1463-0.57920.1253-0.08410.25060.49820.00710.07020.26250.01760.502127.7596-25.56537.5306
95.4909-1.2658-1.93280.88161.24583.6934-0.2436-0.3175-0.12330.29870.0755-0.72390.17860.42360.14110.57850.0753-0.03670.29760.01770.774431.9636-28.80749.486
103.260.93810.26411.8716-3.16446.5709-0.1461-0.41390.22880.9122-0.1255-0.0289-0.7437-0.38840.29750.70970.02370.03450.311-0.07670.375513.9744-15.393513.9906
114.8716-0.57052.94990.2855-1.16744.9451-0.2421-0.2680.60530.8681-0.2669-1.0255-0.36590.13050.47660.8014-0.0788-0.19320.3234-0.01530.750428.805-9.84699.7568
125.32570.27054.39441.7325-0.84258.6456-0.1694-0.27890.65150.4185-0.2149-0.068-0.1998-0.04810.25050.4926-0.00710.010.2078-0.03360.365719.2721-16.47347.2016
139.23093.8407-1.10459.6581-0.89897.6595-0.13610.50761.3598-0.25920.28210.365-0.93630.0457-0.08640.57980.0322-0.05810.29110.02570.478110.7745-9.418-1.9122
144.27251.00320.58185.08911.3954.62980.0091-0.09760.2673-0.0672-0.02250.50080.1512-0.46010.04750.61660.00550.09610.30160.01840.30811.435-26.21111.2963
159.2906-1.4511-0.07294.78660.41684.41310.1169-0.0097-0.5635-0.11890.02460.2810.5753-0.628-0.05340.6615-0.07720.02550.3603-0.00990.24893.2221-28.6176-3.8522
165.08852.6535-0.39872.1427-0.9680.74280.0939-0.53131.57480.15010.1613-0.8281-0.1670.7973-0.12730.4595-0.0721-0.06880.6892-0.05780.935730.140121.098737.9703
171.1046-0.5180.37934.57590.56261.37020.277-1.96161.24850.4391-0.1143-0.257-0.27281.0867-0.17170.5212-0.0416-0.05490.8381-0.26250.657626.939914.563540.7002
182.01874.2531-6.53276.5744-4.67024.9903-0.10080.27310.7668-0.28760.45850.04230.23560.7397-0.37990.4465-0.09010.00310.6802-0.06260.489824.48413.423532.3747
193.92252.5149-2.08294.6322-4.31364.0733-0.53251.5839-0.2621-0.99280.91410.2322-0.1963-1.1008-0.48630.6941-0.16870.06291.0646-0.16080.642824.256812.112328.2375
202.75951.5911-0.15592.8521-1.17340.6194-0.27380.0478-0.2763-0.2590.3277-0.85630.3280.6012-0.02290.3546-0.08380.03340.7832-0.26320.684128.66324.544135.3258
217.31380.67910.02232.784-0.95810.34110.0467-0.1842-0.01880.16320.1532-0.504-0.2580.9884-0.13280.4422-0.1411-0.04761.1081-0.34210.681528.799811.573143.4411
222.52532.69030.08014.6415-1.92242.2842-0.1574-0.04290.6324-0.41680.0216-0.2421-0.11470.77120.08180.6289-0.18140.12880.7582-0.1750.571930.240214.449430.1511
236.66382.16851.29094.9555-0.52993.02720.1319-0.56331.01290.08140.0731-0.4535-0.8440.3235-0.27910.5466-0.04670.03920.3639-0.18950.512612.362413.871137.5984
245.28712.95551.04073.5768-2.27174.43490.3138-0.2572-0.36151.1287-0.1361.56180.3838-0.4273-0.14540.9992-0.23770.19030.5571-0.04010.740636.159920.686728.1453
255.0985-1.6636-0.23922.95920.65643.6269-0.1371-0.4387-0.47450.02640.4350.58630.0468-0.1176-0.32851.03610.07610.22940.4420.18080.5663-8.3786-34.138327.2765
269.3542.1073-1.72153.9121-2.32982.4476-0.1247-0.1843-0.99250.1710.0908-0.18380.30450.00520.06520.7940.01690.03220.3407-0.04280.3283.0448-31.750220.4541
275.8485.60415.4716.26894.94365.0012-0.57540.6739-0.9098-0.69540.86080.0859-0.2852-0.2333-0.31320.7721-0.0784-0.01360.6130.02250.4303-6.4763-29.224113.3146
283.74594.0444-1.11394.4049-1.09180.53510.234-0.21671.60630.04430.10140.8154-0.1308-0.1407-0.34640.84910.04480.16650.34330.01620.3892-1.2203-19.592817.2642
297.6942-3.22261.42213.80040.03421.0105-0.044-0.63730.12410.4270.21180.1084-0.0338-0.3067-0.21360.87720.00890.14840.40510.01740.3007-0.3006-25.351225.5137
306.96472.0613-3.99512.0946-1.77453.3223-0.51380.1201-0.0239-0.11170.28930.19720.6473-0.17130.27530.755-0.02990.03280.3628-0.04610.2957-0.417-31.857715.8063
317.20740.4125-4.74440.41171.23799.00370.44020.97530.584-0.050.05860.54220.1037-0.5599-0.49411.0394-0.03720.03410.44160.13780.4597-14.8085-35.135824.148
Refinement TLS group
IDRefine-IDRefine TLS-IDSelection detailsAuth asym-IDAuth seq-ID
1X-RAY DIFFRACTION1chain 'A' and (resid 5 through 56 )A5 - 56
2X-RAY DIFFRACTION2chain 'A' and (resid 57 through 97 )A57 - 97
3X-RAY DIFFRACTION3chain 'A' and (resid 98 through 122 )A98 - 122
4X-RAY DIFFRACTION4chain 'A' and (resid 123 through 238 )A123 - 238
5X-RAY DIFFRACTION5chain 'A' and (resid 239 through 261 )A239 - 261
6X-RAY DIFFRACTION6chain 'B' and (resid 5 through 32 )B5 - 32
7X-RAY DIFFRACTION7chain 'B' and (resid 33 through 75 )B33 - 75
8X-RAY DIFFRACTION8chain 'B' and (resid 76 through 97 )B76 - 97
9X-RAY DIFFRACTION9chain 'B' and (resid 98 through 122 )B98 - 122
10X-RAY DIFFRACTION10chain 'B' and (resid 123 through 140 )B123 - 140
11X-RAY DIFFRACTION11chain 'B' and (resid 141 through 160 )B141 - 160
12X-RAY DIFFRACTION12chain 'B' and (resid 161 through 180 )B161 - 180
13X-RAY DIFFRACTION13chain 'B' and (resid 181 through 201 )B181 - 201
14X-RAY DIFFRACTION14chain 'B' and (resid 202 through 238 )B202 - 238
15X-RAY DIFFRACTION15chain 'B' and (resid 239 through 261 )B239 - 261
16X-RAY DIFFRACTION16chain 'C' and (resid 3 through 12 )C3 - 12
17X-RAY DIFFRACTION17chain 'C' and (resid 13 through 32 )C13 - 32
18X-RAY DIFFRACTION18chain 'C' and (resid 33 through 39 )C33 - 39
19X-RAY DIFFRACTION19chain 'C' and (resid 40 through 52 )C40 - 52
20X-RAY DIFFRACTION20chain 'C' and (resid 53 through 72 )C53 - 72
21X-RAY DIFFRACTION21chain 'C' and (resid 73 through 82 )C73 - 82
22X-RAY DIFFRACTION22chain 'C' and (resid 83 through 99 )C83 - 99
23X-RAY DIFFRACTION23chain 'C' and (resid 100 through 113 )C100 - 113
24X-RAY DIFFRACTION24chain 'C' and (resid 114 through 119 )C114 - 119
25X-RAY DIFFRACTION25chain 'D' and (resid 3 through 17 )D3 - 17
26X-RAY DIFFRACTION26chain 'D' and (resid 18 through 39 )D18 - 39
27X-RAY DIFFRACTION27chain 'D' and (resid 40 through 52 )D40 - 52
28X-RAY DIFFRACTION28chain 'D' and (resid 53 through 63 )D53 - 63
29X-RAY DIFFRACTION29chain 'D' and (resid 64 through 82 )D64 - 82
30X-RAY DIFFRACTION30chain 'D' and (resid 83 through 113 )D83 - 113
31X-RAY DIFFRACTION31chain 'D' and (resid 114 through 119 )D114 - 119

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