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- PDB-6o9i: Ternary complex of mouse ECD with Fab1 and Fab2 -

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Basic information

Entry
Database: PDB / ID: 6o9i
TitleTernary complex of mouse ECD with Fab1 and Fab2
Components
  • Fab 2 heavy chainFragment antigen-binding
  • Fab1 heavy chainPIKFYVE
  • Fab1 light chainPIKFYVE
  • Fab2 light chain
  • Gastric inhibitory polypeptide receptor
KeywordsIMMUNE SYSTEM / GPCR
Function / homology
Function and homology information


gastric inhibitory peptide receptor activity / glucagon family peptide binding / gastric inhibitory peptide signaling pathway / Glucagon-type ligand receptors / G alpha (s) signalling events / desensitization of G protein-coupled receptor signaling pathway / endocrine pancreas development / response to fatty acid / G protein-coupled peptide receptor activity / peptide hormone binding ...gastric inhibitory peptide receptor activity / glucagon family peptide binding / gastric inhibitory peptide signaling pathway / Glucagon-type ligand receptors / G alpha (s) signalling events / desensitization of G protein-coupled receptor signaling pathway / endocrine pancreas development / response to fatty acid / G protein-coupled peptide receptor activity / peptide hormone binding / neuropeptide signaling pathway / response to axon injury / positive regulation of cAMP-mediated signaling / response to glucose / adenylate cyclase-modulating G protein-coupled receptor signaling pathway / adenylate cyclase-activating G protein-coupled receptor signaling pathway / positive regulation of insulin secretion / response to calcium ion / signaling receptor activity / positive regulation of cytosolic calcium ion concentration / cell surface receptor signaling pathway / plasma membrane
Similarity search - Function
GPCR, family 2, gastric inhibitory polypeptide receptor / G-protein coupled receptors family 2 signature 1. / Hormone receptor domain / GPCR, family 2, extracellular hormone receptor domain / G-protein coupled receptors family 2 profile 1. / Domain present in hormone receptors / GPCR family 2, extracellular hormone receptor domain superfamily / G-protein coupled receptors family 2 signature 2. / GPCR, family 2, secretin-like, conserved site / GPCR, family 2, secretin-like ...GPCR, family 2, gastric inhibitory polypeptide receptor / G-protein coupled receptors family 2 signature 1. / Hormone receptor domain / GPCR, family 2, extracellular hormone receptor domain / G-protein coupled receptors family 2 profile 1. / Domain present in hormone receptors / GPCR family 2, extracellular hormone receptor domain superfamily / G-protein coupled receptors family 2 signature 2. / GPCR, family 2, secretin-like, conserved site / GPCR, family 2, secretin-like / 7 transmembrane receptor (Secretin family) / GPCR, family 2-like / G-protein coupled receptors family 2 profile 2. / Immunoglobulins / Immunoglobulin-like / Sandwich / Mainly Beta
Similarity search - Domain/homology
Gastric inhibitory polypeptide receptor
Similarity search - Component
Biological speciesHomo sapiens (human)
Mus musculus (house mouse)
MethodX-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 2.6 Å
AuthorsMin, X. / Wang, Z.
CitationJournal: Mabs / Year: 2020
Title: Molecular mechanism of an antagonistic antibody against glucose-dependent insulinotropic polypeptide receptor.
Authors: Min, X. / Yie, J. / Wang, J. / Chung, B.C. / Huang, C.S. / Xu, H. / Yang, J. / Deng, L. / Lin, J. / Chen, Q. / Abbott, C.M. / Gundel, C. / Thibault, S.A. / Meng, T. / Bates, D.L. / Lloyd, D. ...Authors: Min, X. / Yie, J. / Wang, J. / Chung, B.C. / Huang, C.S. / Xu, H. / Yang, J. / Deng, L. / Lin, J. / Chen, Q. / Abbott, C.M. / Gundel, C. / Thibault, S.A. / Meng, T. / Bates, D.L. / Lloyd, D.J. / Veniant, M.M. / Wang, Z.
History
DepositionMar 13, 2019Deposition site: RCSB / Processing site: RCSB
Revision 1.0Jan 22, 2020Provider: repository / Type: Initial release
Revision 2.0Feb 19, 2020Group: Advisory / Atomic model ...Advisory / Atomic model / Data collection / Derived calculations / Non-polymer description / Polymer sequence / Refinement description / Structure summary
Category: atom_site / atom_site_anisotrop ...atom_site / atom_site_anisotrop / chem_comp / diffrn / entity / entity_poly / entity_poly_seq / pdbx_distant_solvent_atoms / pdbx_entry_details / pdbx_nonpoly_scheme / pdbx_poly_seq_scheme / pdbx_refine_tls / pdbx_struct_assembly / pdbx_struct_assembly_gen / pdbx_struct_assembly_prop / pdbx_struct_oper_list / pdbx_struct_sheet_hbond / pdbx_validate_close_contact / pdbx_validate_rmsd_angle / pdbx_validate_rmsd_bond / pdbx_validate_symm_contact / pdbx_validate_torsion / refine / refine_hist / refine_ls_restr / refine_ls_shell / reflns / reflns_shell / software / struct_conf / struct_conn / struct_conn_type / struct_mon_prot_cis / struct_sheet / struct_sheet_order / struct_sheet_range / struct_site / struct_site_gen
Item: _chem_comp.formula / _chem_comp.formula_weight ..._chem_comp.formula / _chem_comp.formula_weight / _chem_comp.id / _chem_comp.mon_nstd_flag / _chem_comp.name / _diffrn.ambient_temp / _entity.formula_weight / _entity.pdbx_number_of_molecules / _entity_poly.nstd_monomer / _entity_poly.pdbx_seq_one_letter_code / _entity_poly_seq.mon_id / _pdbx_distant_solvent_atoms.auth_asym_id / _pdbx_distant_solvent_atoms.auth_seq_id / _pdbx_distant_solvent_atoms.neighbor_macromolecule_distance / _pdbx_poly_seq_scheme.auth_mon_id / _pdbx_poly_seq_scheme.mon_id / _pdbx_poly_seq_scheme.pdb_mon_id / _pdbx_refine_tls.L[1][1] / _pdbx_refine_tls.L[1][2] / _pdbx_refine_tls.L[1][3] / _pdbx_refine_tls.L[2][2] / _pdbx_refine_tls.L[2][3] / _pdbx_refine_tls.L[3][3] / _pdbx_refine_tls.S[1][1] / _pdbx_refine_tls.S[1][2] / _pdbx_refine_tls.S[1][3] / _pdbx_refine_tls.S[2][1] / _pdbx_refine_tls.S[2][2] / _pdbx_refine_tls.S[2][3] / _pdbx_refine_tls.S[3][1] / _pdbx_refine_tls.S[3][2] / _pdbx_refine_tls.S[3][3] / _pdbx_refine_tls.T[1][1] / _pdbx_refine_tls.T[1][2] / _pdbx_refine_tls.T[1][3] / _pdbx_refine_tls.T[2][2] / _pdbx_refine_tls.T[2][3] / _pdbx_refine_tls.T[3][3] / _pdbx_refine_tls.origin_x / _pdbx_refine_tls.origin_y / _pdbx_refine_tls.origin_z / _pdbx_struct_assembly.details / _pdbx_struct_assembly.method_details / _pdbx_struct_assembly_prop.value / _refine.B_iso_max / _refine.B_iso_mean / _refine.B_iso_min / _refine.ls_R_factor_R_free / _refine.ls_R_factor_R_work / _refine.ls_R_factor_obs / _refine.pdbx_overall_phase_error / _refine.pdbx_starting_model / _refine_hist.number_atoms_solvent / _refine_hist.number_atoms_total / _refine_hist.pdbx_B_iso_mean_ligand / _refine_hist.pdbx_B_iso_mean_solvent / _refine_hist.pdbx_number_atoms_protein / _refine_ls_restr.dev_ideal / _refine_ls_restr.number / _refine_ls_shell.R_factor_R_free / _refine_ls_shell.R_factor_R_work / _refine_ls_shell.d_res_low / _refine_ls_shell.number_reflns_all / _refine_ls_shell.pdbx_total_number_of_bins_used / _reflns.B_iso_Wilson_estimate / _reflns.pdbx_number_measured_all / _reflns_shell.number_measured_all / _reflns_shell.pdbx_netI_over_sigmaI_obs / _software.classification / _software.name / _software.version / _struct_mon_prot_cis.pdbx_omega_angle / _struct_site_gen.auth_asym_id / _struct_site_gen.auth_seq_id / _struct_site_gen.label_asym_id / _struct_site_gen.pdbx_num_res / _struct_site_gen.site_id
Description: Ligand identity
Details: Replace the N-terminal Gln with post-translational modified residue PCA.
Provider: author / Type: Coordinate replacement
Revision 2.1Feb 26, 2020Group: Database references / Category: citation / citation_author
Item: _citation.journal_volume / _citation.year / _citation_author.identifier_ORCID
Revision 2.2Apr 1, 2020Group: Database references / Source and taxonomy / Structure summary
Category: entity / entity_name_com ...entity / entity_name_com / entity_src_gen / struct_ref / struct_ref_seq / struct_ref_seq_dif
Item: _entity.pdbx_description / _entity_src_gen.gene_src_common_name ..._entity.pdbx_description / _entity_src_gen.gene_src_common_name / _entity_src_gen.pdbx_gene_src_gene / _entity_src_gen.pdbx_gene_src_ncbi_taxonomy_id / _entity_src_gen.pdbx_gene_src_scientific_name / _struct_ref.db_code / _struct_ref.db_name / _struct_ref.pdbx_align_begin / _struct_ref.pdbx_db_accession / _struct_ref.pdbx_seq_one_letter_code / _struct_ref_seq.db_align_beg / _struct_ref_seq.db_align_end / _struct_ref_seq.pdbx_auth_seq_align_beg / _struct_ref_seq.pdbx_db_accession / _struct_ref_seq.seq_align_beg
Revision 2.3Jun 10, 2020Group: Database references / Category: citation / Item: _citation.year
Revision 2.4Oct 11, 2023Group: Data collection / Database references / Refinement description
Category: chem_comp_atom / chem_comp_bond ...chem_comp_atom / chem_comp_bond / database_2 / pdbx_initial_refinement_model
Item: _database_2.pdbx_DOI / _database_2.pdbx_database_accession

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Assembly

Deposited unit
A: Fab1 heavy chain
B: Fab1 light chain
C: Gastric inhibitory polypeptide receptor
D: Fab 2 heavy chain
E: Fab2 light chain
hetero molecules


Theoretical massNumber of molelcules
Total (without water)112,93110
Polymers112,6205
Non-polymers3105
Water3,855214
1


  • Idetical with deposited unit
  • defined by author
  • Evidence: gel filtration
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area10690 Å2
ΔGint-40 kcal/mol
Surface area42880 Å2
Unit cell
Length a, b, c (Å)41.430, 43.680, 133.820
Angle α, β, γ (deg.)87.860, 88.380, 81.170
Int Tables number1
Space group name H-MP1

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Components

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Protein , 1 types, 1 molecules C

#3: Protein Gastric inhibitory polypeptide receptor / / GIP-R / Glucose-dependent insulinotropic polypeptide receptor


Mass: 15673.128 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Mus musculus (house mouse) / Gene: Gipr / Production host: Escherichia coli (E. coli) / References: UniProt: Q0P543

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Antibody , 4 types, 4 molecules ABDE

#1: Antibody Fab1 heavy chain / PIKFYVE


Mass: 24085.227 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human) / Production host: Cricetulus griseus (Chinese hamster)
#2: Antibody Fab1 light chain / PIKFYVE


Mass: 24223.713 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human) / Production host: Cricetulus griseus (Chinese hamster)
#4: Antibody Fab 2 heavy chain / Fragment antigen-binding


Mass: 24468.541 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human) / Production host: Cricetulus griseus (Chinese hamster)
#5: Antibody Fab2 light chain


Mass: 24169.830 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human) / Production host: Cricetulus griseus (Chinese hamster)

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Non-polymers , 2 types, 219 molecules

#6: Chemical
ChemComp-EDO / 1,2-ETHANEDIOL / ETHYLENE GLYCOL / Ethylene glycol


Mass: 62.068 Da / Num. of mol.: 5 / Source method: obtained synthetically / Formula: C2H6O2
#7: Water ChemComp-HOH / water / Water


Mass: 18.015 Da / Num. of mol.: 214 / Source method: isolated from a natural source / Formula: H2O

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Details

Has ligand of interestN

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Experimental details

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Experiment

ExperimentMethod: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

CrystalDensity Matthews: 2.12 Å3/Da / Density % sol: 42.05 %
Crystal growTemperature: 293 K / Method: vapor diffusion, sitting drop / pH: 5.5
Details: 0.1 M Bis-Tris, pH 5.5, 0.2 M MgCl2 and 25% Peg3350

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Data collection

DiffractionMean temperature: 80 K / Serial crystal experiment: N
Diffraction sourceSource: SYNCHROTRON / Site: ALS / Beamline: 5.0.2 / Wavelength: 1 Å
DetectorType: ADSC QUANTUM 315r / Detector: CCD / Date: Feb 12, 2016
RadiationMonochromator: Double-crystal, Si(111) / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelengthWavelength: 1 Å / Relative weight: 1
ReflectionResolution: 2.6→19.76 Å / Num. obs: 28394 / % possible obs: 99.7 % / Redundancy: 4.8 % / Biso Wilson estimate: 35.76 Å2 / CC1/2: 0.985 / Rmerge(I) obs: 0.192 / Rpim(I) all: 0.097 / Rrim(I) all: 0.216 / Net I/σ(I): 7.3 / Num. measured all: 137402
Reflection shellResolution: 2.6→2.72 Å / Redundancy: 4.8 % / Rmerge(I) obs: 0.967 / Num. measured all: 16771 / Num. unique obs: 3465 / CC1/2: 0.629 / Rpim(I) all: 0.491 / Rrim(I) all: 1.087 / Net I/σ(I) obs: 1.7 / % possible all: 99.9

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Processing

Software
NameVersionClassification
PHENIXdev-2328_1692refinement
XDSdata reduction
Aimless0.5.29data scaling
PDB_EXTRACT3.24data extraction
PHASERphasing
RefinementMethod to determine structure: MOLECULAR REPLACEMENT
Starting model: 2QKH

2qkh


Resolution: 2.6→19.76 Å / SU ML: 0.31 / Cross valid method: THROUGHOUT / σ(F): 1.97 / Phase error: 28.28
RfactorNum. reflection% reflection
Rfree0.2663 1559 5.49 %
Rwork0.2163 --
obs0.2191 28391 99.94 %
Solvent computationShrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å
Displacement parametersBiso max: 169.22 Å2 / Biso mean: 54.6881 Å2 / Biso min: 9.87 Å2
Refinement stepCycle: final / Resolution: 2.6→19.76 Å
ProteinNucleic acidLigandSolventTotal
Num. atoms7326 0 20 214 7560
Biso mean--57.97 35.19 -
Num. residues----950
Refine LS restraints
Refine-IDTypeDev idealNumber
X-RAY DIFFRACTIONf_bond_d0.0057564
X-RAY DIFFRACTIONf_angle_d0.80310303
X-RAY DIFFRACTIONf_chiral_restr0.0451140
X-RAY DIFFRACTIONf_plane_restr0.0061303
X-RAY DIFFRACTIONf_dihedral_angle_d16.2884490
LS refinement shell

Refine-ID: X-RAY DIFFRACTION / Rfactor Rfree error: 0 / % reflection obs: 100 %

Resolution (Å)Rfactor RfreeNum. reflection RfreeRfactor RworkNum. reflection Rwork
2.6-2.68370.32761200.30192425
2.6837-2.77940.31851520.29562451
2.7794-2.89040.31581360.28852421
2.8904-3.02150.32831630.27622424
3.0215-3.18010.33981310.26542471
3.1801-3.37850.33081570.242435
3.3785-3.63780.25971550.22112427
3.6378-4.00120.25441580.19422419
4.0012-4.57390.23311380.17622452
4.5739-5.73910.20591120.16172455
5.7391-100.20491370.18262452
Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

IDL11 (°2)L12 (°2)L13 (°2)L22 (°2)L23 (°2)L33 (°2)S11 (Å °)S12 (Å °)S13 (Å °)S21 (Å °)S22 (Å °)S23 (Å °)S31 (Å °)S32 (Å °)S33 (Å °)T11 (Å2)T12 (Å2)T13 (Å2)T22 (Å2)T23 (Å2)T33 (Å2)Origin x (Å)Origin y (Å)Origin z (Å)
13.7685-1.2248-0.61884.61451.60083.89410.269-0.2304-0.39640.3854-0.23590.12720.241-0.041-0.09310.4425-0.04620.03730.2435-0.0520.24477.6413-55.01587.877
21.74580.5275-2.15813.0799-0.34392.69060.12230.4790.0130.1859-0.27831.2261-0.0237-0.7436-0.4345-0.0245-0.10390.50420.7056-0.25290.7446-6.003-48.25218.0587
31.2892-2.26060.88754.2456-0.83423.37570.1264-0.52980.39810.14370.068-0.37160.07290.322-0.14710.4184-0.12520.1320.4332-0.09110.314111.8721-45.198714.4417
42.96633.5707-1.52646.5102-4.85614.96830.3877-0.07620.26941.01450.02820.7387-0.1488-0.8589-0.36620.8167-0.05910.20070.677-0.15130.7211-7.682-48.583519.4507
52.21370.39150.01276.36211.60421.3054-0.1782-0.2259-0.057-0.58240.3332-0.56980.07510.2165-0.10980.25690.0417-0.04840.31340.04020.175421.2516-76.5283-6.2464
64.70250.0121.19655.0847-0.94984.06390.1648-0.309-0.09410.3095-0.18840.24260.2501-0.3387-0.01740.2128-0.01590.03770.1845-0.03690.17311.6086-77.6928-2.436
71.59031.0581-1.37387.97091.22584.07870.0685-0.2048-0.41060.4629-0.5387-0.07820.2865-0.78450.34280.25890.1004-0.05250.23790.03370.160115.1552-85.4839-5.1971
81.3867-0.71010.15722.38840.91441.91840.0104-0.1041-0.0266-0.39210.0598-0.13740.09780.0421-0.13050.21470.0161-0.0110.1841-0.01130.175715.9853-77.1393-11.9038
92.55980.5018-1.98046.0354-2.14412.997-0.0787-0.0394-0.1089-0.37440.1276-0.23810.3210.35790.04160.13170.073-0.12190.2537-0.01640.251427.3305-83.7793-35.4906
101.7989-0.9352-0.76464.3780.20555.9464-0.0206-0.06650.17760.04490.1777-0.14210.0150.4398-0.10350.08980.0208-0.03280.2742-0.00640.255228.4513-82.4504-34.1216
113.48120.8324-2.12792.5261-1.54036.9919-0.16990.155-0.1013-0.4229-0.12830.47060.4869-0.44960.21750.21830.0131-0.04270.2383-0.04880.332-2.9765-67.7578-23.0607
123.17850.5040.4571.79270.40395.0110.0073-0.06090.3021-0.0516-0.00980.235-0.2878-0.397-0.02520.1870.067-0.05080.1583-0.03310.24292.1447-61.4139-14.492
130.7946-0.90910.4092.0798-1.84257.5572-0.2498-0.047-0.11250.3232-0.37050.0098-0.76470.04380.4650.0925-0.0157-0.03110.2029-0.0360.28483.88-67.8943-16.9519
140.7924-0.11220.33852.5052-1.05033.24050.53380.19510.1484-0.9142-0.25740.53170.381-0.3793-0.2073-0.01170.3288-0.2160.09760.21390.35025.9157-70.7519-38.138
152.2696-3.04310.67515.8639-0.23572.43480.0277-0.2388-0.3125-0.27980.01980.56080.4481-0.3081-0.00460.1909-0.0059-0.04170.2342-0.00330.335817.8045-89.6465-43.4467
163.3589-1.7105-0.87293.86410.62191.6456-0.45670.2178-0.599-0.01490.22890.76710.46910.01860.07590.24530.0458-0.17310.26470.03930.214217.921-88.6465-43.1432
173.697-1.5909-1.61284.121.96342.09380.32481.39350.3235-1.0117-0.5271-0.0528-0.5370.07150.17660.5826-0.0187-0.11440.5191-0.07580.26516.0642-85.9043-53.0902
184.85064.51846.47716.4675.34728.84180.3992-1.131-0.7480.4121-0.40130.47090.0578-1.2408-0.22510.6955-0.12970.19730.540.04240.66224.7927-48.347539.3814
191.9595-0.34361.61972.80071.27764.12440.19130.3865-0.12910.19360.5671-0.6360.93090.1705-0.51180.76970.07370.02360.55320.13420.471717.9297-48.078845.0473
202.897-2.0329-0.04251.4957-0.24121.06760.00790.4748-1.0922-0.2619-0.02870.14961.1045-0.57750.11780.9121-0.22030.15320.3122-0.15780.5457.874-52.962827.4307
218.6854-2.9539-1.93162.36171.54595.88560.41230.10580.35180.67280.046-0.5887-0.88090.1001-0.42810.69840.06940.00060.3357-0.01070.332214.1599-36.150438.4563
225.11680.47681.27927.50511.75315.2075-0.07120.0713-0.90770.08490.3842-0.63060.66690.3427-0.24180.47740.03760.04810.3193-0.04630.46219.7274-47.04732.3298
234.12010.66862.79812.6335-2.61965.64520.32250.19330.1765-1.6665-0.18680.00710.19910.06710.01091.036-0.2148-0.09130.7263-0.11980.587225.1829-37.316345.3031
245.83750.5652.29311.46631.49074.69110.1995-0.7012-0.36670.2425-0.0232-0.14950.9943-0.121-0.29350.4716-0.03460.10620.31810.04510.25869.4329-41.711235.259
253.1806-3.7443-0.27236.58253.07593.51410.75810.03060.01670.4321-0.7763-0.6708-0.26410.91570.14330.7581-0.26150.10870.6736-0.03570.532317.1549-35.806567.3522
268.6199-5.64415.56537.4916-3.48968.5801-0.1451-1.3283-0.080.63250.70.51-0.5376-0.8061-0.80610.947-0.1260.31370.4579-0.02730.45026.0535-17.099269.7934
273.05330.6084-0.16161.198-1.24711.76010.47230.7502-0.2370.15480.0917-0.47650.04320.3252-0.48330.6081-0.1897-0.03020.5659-0.27840.500611.0691-33.400663.0171
280.1422-0.0131-0.2710.2527-0.7412.81180.6137-0.1071-0.05080.7238-0.33890.5521-0.33360.034-0.33350.7063-0.22360.07460.427-0.10390.5187-1.0808-25.545965.3325
294.91782.11330.70544.26840.22788.65480.18310.8303-0.16530.14550.1734-0.4644-0.13361.8712-0.23460.3846-0.06850.08240.7968-0.11440.539115.9936-30.361758.3199
301.3523-0.40191.51246.87823.21623.70020.4731-0.84310.5897-0.6678-0.51040.8021-0.6011-0.1511-0.12780.7167-0.2130.25630.5906-0.1530.4780.3755-18.395473.2154
312.0682-1.6398-0.46044.574-0.99997.0401-0.45690.2575-0.26460.48450.211-0.8391-0.12690.60960.36010.7448-0.08410.08290.4983-0.17730.56527.7268-36.41767.5935
321.4041-2.9915-1.13336.7142.47080.9639-0.5972-0.4764-0.30.51570.9924-0.19110.05480.9663-0.32550.873-0.07480.28120.477-0.08160.56987.9376-30.836974.4464
331.98810.357-0.77262.28220.2763.9220.4093-0.12650.4814-0.3750.1001-0.2741-1.0289-0.0439-0.51140.8839-0.1480.25120.3641-0.0560.43996.5364-23.68526.0075
343.2556-1.35730.14812.27451.73373.68130.1011-0.12920.1082-0.2482-0.08970.1747-0.5792-0.25840.01990.5544-0.03880.15420.28270.00370.3491.8066-29.606429.2744
352.4719-0.1362-0.09032.60462.77012.9194-0.0948-0.13740.2230.46340.092-1.0767-1.4309-0.5705-0.06491.14850.0440.16150.4248-0.09730.61010.8661-14.955846.0275
361.11810.6699-0.2651.90270.75563.24690.2753-0.1004-0.0820.2815-0.0365-0.0991-0.4670.6904-0.35191.08-0.44740.10650.7603-0.18190.421714.6447-16.756862.3968
373.8225-0.03233.00522.30980.29832.4126-0.92950.2119-0.25910.0340.3356-0.0323-0.31140.3050.48520.6975-0.11640.27940.5702-0.10110.40719.6691-19.233353.8354
380.73850.31470.33821.96821.88661.8096-0.01580.05350.57690.08440.05010.0475-1.09560.98560.07571.6945-0.69070.06240.9825-0.12240.376917.182-9.453361.5218
Refinement TLS group
IDRefine-IDRefine TLS-IDSelection detailsAuth asym-IDAuth seq-ID
1X-RAY DIFFRACTION1chain 'C' and (resid 4 through 54 )C4 - 54
2X-RAY DIFFRACTION2chain 'C' and (resid 55 through 68 )C55 - 68
3X-RAY DIFFRACTION3chain 'C' and (resid 69 through 86 )C69 - 86
4X-RAY DIFFRACTION4chain 'C' and (resid 87 through 109 )C87 - 109
5X-RAY DIFFRACTION5chain 'A' and (resid 1 through 33 )A1 - 33
6X-RAY DIFFRACTION6chain 'A' and (resid 34 through 76 )A34 - 76
7X-RAY DIFFRACTION7chain 'A' and (resid 77 through 91 )A77 - 91
8X-RAY DIFFRACTION8chain 'A' and (resid 92 through 124 )A92 - 124
9X-RAY DIFFRACTION9chain 'A' and (resid 125 through 162 )A125 - 162
10X-RAY DIFFRACTION10chain 'A' and (resid 163 through 219 )A163 - 219
11X-RAY DIFFRACTION11chain 'B' and (resid 1 through 25 )B1 - 25
12X-RAY DIFFRACTION12chain 'B' and (resid 26 through 81 )B26 - 81
13X-RAY DIFFRACTION13chain 'B' and (resid 82 through 107 )B82 - 107
14X-RAY DIFFRACTION14chain 'B' and (resid 108 through 119 )B108 - 119
15X-RAY DIFFRACTION15chain 'B' and (resid 120 through 169 )B120 - 169
16X-RAY DIFFRACTION16chain 'B' and (resid 170 through 204 )B170 - 204
17X-RAY DIFFRACTION17chain 'B' and (resid 205 through 217 )B205 - 217
18X-RAY DIFFRACTION18chain 'D' and (resid 1 through 9 )D1 - 9
19X-RAY DIFFRACTION19chain 'D' and (resid 10 through 25 )D10 - 25
20X-RAY DIFFRACTION20chain 'D' and (resid 26 through 33 )D26 - 33
21X-RAY DIFFRACTION21chain 'D' and (resid 34 through 44 )D34 - 44
22X-RAY DIFFRACTION22chain 'D' and (resid 45 through 84 )D45 - 84
23X-RAY DIFFRACTION23chain 'D' and (resid 85 through 91 )D85 - 91
24X-RAY DIFFRACTION24chain 'D' and (resid 92 through 119 )D92 - 119
25X-RAY DIFFRACTION25chain 'D' and (resid 120 through 129 )D120 - 129
26X-RAY DIFFRACTION26chain 'D' and (resid 130 through 146 )D130 - 146
27X-RAY DIFFRACTION27chain 'D' and (resid 147 through 160 )D147 - 160
28X-RAY DIFFRACTION28chain 'D' and (resid 161 through 171 )D161 - 171
29X-RAY DIFFRACTION29chain 'D' and (resid 172 through 184 )D172 - 184
30X-RAY DIFFRACTION30chain 'D' and (resid 185 through 199 )D185 - 199
31X-RAY DIFFRACTION31chain 'D' and (resid 200 through 208 )D200 - 208
32X-RAY DIFFRACTION32chain 'D' and (resid 209 through 218 )D209 - 218
33X-RAY DIFFRACTION33chain 'E' and (resid 1 through 37 )E1 - 37
34X-RAY DIFFRACTION34chain 'E' and (resid 38 through 107 )E38 - 107
35X-RAY DIFFRACTION35chain 'E' and (resid 108 through 119 )E108 - 119
36X-RAY DIFFRACTION36chain 'E' and (resid 120 through 155 )E120 - 155
37X-RAY DIFFRACTION37chain 'E' and (resid 156 through 180 )E156 - 180
38X-RAY DIFFRACTION38chain 'E' and (resid 181 through 216 )E181 - 216

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