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- PDB-6o6j: Crystal structure of the LjCASTOR gating ring in the Ca2+ and Na+... -
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Open data
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Basic information
Entry | Database: PDB / ID: 6o6j | ||||||||||||
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Title | Crystal structure of the LjCASTOR gating ring in the Ca2+ and Na+ condition | ||||||||||||
![]() | Ion channel CASTOR | ||||||||||||
![]() | TRANSPORT PROTEIN / calcium channel / RCK domain / gating ring / CASTOR / membrane protein | ||||||||||||
Function / homology | CASTOR/POLLUX/SYM8 ion channel, conserved domain / Ion channel CASTOR/POLLUX/SYM8-like / Castor and Pollux, part of voltage-gated ion channel / monoatomic ion transmembrane transport / nuclear membrane / Ion channel CASTOR![]() | ||||||||||||
Biological species | ![]() ![]() | ||||||||||||
Method | ![]() ![]() ![]() | ||||||||||||
![]() | Jiang, Y. / Kim, S. | ||||||||||||
Funding support | ![]()
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![]() | ![]() Title: Ca2+-regulated Ca2+channels with an RCK gating ring control plant symbiotic associations. Authors: Kim, S. / Zeng, W. / Bernard, S. / Liao, J. / Venkateshwaran, M. / Ane, J.M. / Jiang, Y. | ||||||||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 158.4 KB | Display | ![]() |
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PDB format | ![]() | 97.5 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 3.1 MB | Display | ![]() |
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Full document | ![]() | 3.1 MB | Display | |
Data in XML | ![]() | 24 KB | Display | |
Data in CIF | ![]() | 37.9 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
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Links
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Assembly
Deposited unit | ![]()
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1 | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 61956.867 Da / Num. of mol.: 1 / Fragment: gating ring (UNP residues 312-853) Source method: isolated from a genetically manipulated source Details: Jean-Michel Ane, University of Wisconsin - Madison, Madison, WI 53706, USA. Source: (gene. exp.) ![]() ![]() ![]() ![]() | ||||||
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#2: Chemical | #3: Chemical | ChemComp-MG / | #4: Chemical | #5: Water | ChemComp-HOH / | |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.83 Å3/Da / Density % sol: 56.47 % / Description: a thin rectangular prism with smooth edges |
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Crystal grow | Temperature: 293 K / Method: vapor diffusion, sitting drop / pH: 8.2 Details: 21% w/v PEG550 MME, 50 mM magnesium acetate, 100 mM Tris, pH 8.2 PH range: 7.5-8.5 / Temp details: 293 K |
-Data collection
Diffraction | Mean temperature: 100 K / Serial crystal experiment: N |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: ADSC QUANTUM 315r / Detector: CCD / Date: Jul 11, 2013 |
Radiation | Monochromator: Rosenbaum-Rock double crystal Si (111) / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.94936 Å / Relative weight: 1 |
Reflection | Resolution: 1.6→50 Å / Num. obs: 160841 / % possible obs: 98.75 % / Redundancy: 5 % / Biso Wilson estimate: 17.93 Å2 / Net I/σ(I): 34.54 |
Reflection shell | Resolution: 1.6→1.63 Å / Redundancy: 3.8 % / Rmerge(I) obs: 1.201 / Mean I/σ(I) obs: 1.983 / Num. unique obs: 7449 / CC1/2: 0.405 / Rpim(I) all: 0.671 / Rrim(I) all: 1.382 / Rsym value: 1.201 / Χ2: 1.149 / % possible all: 90.21 |
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Processing
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Refinement | Method to determine structure: ![]()
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 25.61 Å2 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 1.6→39.6 Å
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Refine LS restraints |
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LS refinement shell |
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