rRNA N-glycosylase / rRNA N-glycosylase activity / metabolic process / defense response / toxin activity / negative regulation of translation Similarity search - Function
Single alpha-helices involved in coiled-coils or other helix-helix interfaces - #890 / Single alpha-helices involved in coiled-coils or other helix-helix interfaces / Helix non-globular / Special Similarity search - Domain/homology
Type: Bruker AVANCE III / Manufacturer: Bruker / Model: AVANCE III / Field strength: 700 MHz / Details: equipped with cryoprobe
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Processing
NMR software
Name
Version
Developer
Classification
TopSpin
4
BrukerBiospin
collection
TopSpin
4
BrukerBiospin
processing
CARA
KellerandWuthrich
chemicalshiftassignment
CARA
KellerandWuthrich
peakpicking
CYANA
3.9
Guntert, MumenthalerandWuthrich
structurecalculation
CNS
1.2
Brunger, Adams, Clore, Gros, NilgesandRead
refinement
Refinement
Method: simulated annealing / Software ordinal: 6 Details: Structures calculated by torsion angle dynamics followed by refinement and energy minimisation in explicit solvent.
NMR representative
Selection criteria: lowest energy
NMR ensemble
Conformer selection criteria: structures with acceptable covalent geometry Conformers calculated total number: 50 / Conformers submitted total number: 20
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