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- PDB-6o2b: Crystal structure of 4493 Fab in complex with circumsporozoite pr... -

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Basic information

Entry
Database: PDB / ID: 6o2b
TitleCrystal structure of 4493 Fab in complex with circumsporozoite protein DND and anti-kappa VHH domain
Components
  • 4493 Fab heavy chain
  • 4493 Kappa light chain
  • Anti-kappa VHH domain
  • Circumsporozoite protein
KeywordsIMMUNE SYSTEM / Malaria / antibody
Function / homology
Function and homology information


entry into host cell by a symbiont-containing vacuole / side of membrane / cell surface / plasma membrane / cytoplasm
Similarity search - Function
Plasmodium circumsporozoite protein / Thrombospondin type 1 domain / Thrombospondin type-1 (TSP1) repeat superfamily / Thrombospondin type-1 (TSP1) repeat profile. / Thrombospondin type 1 repeats / Thrombospondin type-1 (TSP1) repeat
Similarity search - Domain/homology
ACETATE ION / Circumsporozoite protein
Similarity search - Component
Biological speciesHomo sapiens (human)
Lama glama (llama)
Plasmodium falciparum (malaria parasite P. falciparum)
MethodX-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 2.1 Å
AuthorsScally, S.W. / Bosch, A. / Prieto, K. / Murugan, R. / Wardemann, H. / Julien, J.P.
Funding support United States, 1items
OrganizationGrant numberCountry
Bill & Melinda Gates Foundation United States
CitationJournal: Nat. Med. / Year: 2020
Title: Evolution of protective human antibodies against Plasmodium falciparum circumsporozoite protein repeat motifs.
Authors: Murugan, R. / Scally, S.W. / Costa, G. / Mustafa, G. / Thai, E. / Decker, T. / Bosch, A. / Prieto, K. / Levashina, E.A. / Julien, J.P. / Wardemann, H.
History
DepositionFeb 22, 2019Deposition site: RCSB / Processing site: RCSB
Revision 1.0Jul 1, 2020Provider: repository / Type: Initial release
Revision 1.1Jul 29, 2020Group: Database references / Category: citation / citation_author
Item: _citation.journal_volume / _citation.page_first ..._citation.journal_volume / _citation.page_first / _citation.page_last / _citation_author.identifier_ORCID
Revision 1.2Apr 3, 2024Group: Data collection / Database references / Refinement description
Category: chem_comp_atom / chem_comp_bond ...chem_comp_atom / chem_comp_bond / database_2 / pdbx_initial_refinement_model / struct_ncs_dom_lim
Item: _database_2.pdbx_DOI / _database_2.pdbx_database_accession ..._database_2.pdbx_DOI / _database_2.pdbx_database_accession / _struct_ncs_dom_lim.beg_auth_comp_id / _struct_ncs_dom_lim.beg_label_asym_id / _struct_ncs_dom_lim.beg_label_comp_id / _struct_ncs_dom_lim.beg_label_seq_id / _struct_ncs_dom_lim.end_auth_comp_id / _struct_ncs_dom_lim.end_label_asym_id / _struct_ncs_dom_lim.end_label_comp_id / _struct_ncs_dom_lim.end_label_seq_id

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Assembly

Deposited unit
A: 4493 Fab heavy chain
B: 4493 Kappa light chain
C: 4493 Fab heavy chain
D: 4493 Kappa light chain
H: 4493 Fab heavy chain
I: 4493 Kappa light chain
O: 4493 Fab heavy chain
P: 4493 Kappa light chain
E: Anti-kappa VHH domain
J: Anti-kappa VHH domain
M: Anti-kappa VHH domain
F: Circumsporozoite protein
G: Circumsporozoite protein
K: Circumsporozoite protein
L: Circumsporozoite protein
N: Anti-kappa VHH domain
hetero molecules


Theoretical massNumber of molelcules
Total (without water)239,91525
Polymers239,38116
Non-polymers5349
Water23,8701325
1
A: 4493 Fab heavy chain
B: 4493 Kappa light chain
F: Circumsporozoite protein
N: Anti-kappa VHH domain
hetero molecules


Theoretical massNumber of molelcules
Total (without water)60,0257
Polymers59,8454
Non-polymers1803
Water724
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
2
C: 4493 Fab heavy chain
D: 4493 Kappa light chain
M: Anti-kappa VHH domain
G: Circumsporozoite protein
hetero molecules


Theoretical massNumber of molelcules
Total (without water)60,0227
Polymers59,8454
Non-polymers1773
Water724
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
3
H: 4493 Fab heavy chain
I: 4493 Kappa light chain
E: Anti-kappa VHH domain
K: Circumsporozoite protein
hetero molecules


Theoretical massNumber of molelcules
Total (without water)59,9045
Polymers59,8454
Non-polymers591
Water724
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
4
O: 4493 Fab heavy chain
P: 4493 Kappa light chain
J: Anti-kappa VHH domain
L: Circumsporozoite protein
hetero molecules


Theoretical massNumber of molelcules
Total (without water)59,9636
Polymers59,8454
Non-polymers1182
Water724
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Unit cell
Length a, b, c (Å)83.073, 93.405, 94.164
Angle α, β, γ (deg.)82.740, 76.860, 64.450
Int Tables number1
Space group name H-MP1
Noncrystallographic symmetry (NCS)NCS domain:
IDEns-IDDetails
11(chain A and (resid 1 through 18 or resid 20 through 214))
21(chain C and (resid 1 through 18 or resid 20 through 127 or resid 135 through 214))
31(chain H and (resid 1 through 18 or resid 20 through 127 or resid 135 through 214))
41(chain O and (resid 1 through 18 or resid 20 through 127 or resid 135 through 214))
12(chain B and (resid 1 through 23 or (resid 24...
22chain D
32chain I
42(chain P and (resid 1 through 23 or (resid 24...
13(chain E and resid 2 through 112)
23chain J
33(chain M and resid 2 through 112)
43(chain N and resid 2 through 112)
14chain F
24chain G
34chain K
44chain L

NCS domain segments:
Dom-IDComponent-IDEns-IDBeg auth comp-IDBeg label comp-IDEnd auth comp-IDEnd label comp-IDSelection detailsAuth asym-IDLabel asym-IDAuth seq-IDLabel seq-ID
111GLUGLULEULEU(chain A and (resid 1 through 18 or resid 20 through 214))AA1 - 181 - 18
121LEULEULYSLYS(chain A and (resid 1 through 18 or resid 20 through 214))AA20 - 21420 - 223
211GLUGLULEULEU(chain C and (resid 1 through 18 or resid 20 through 127 or resid 135 through 214))CC1 - 181 - 18
221LEULEUSERSER(chain C and (resid 1 through 18 or resid 20 through 127 or resid 135 through 214))CC20 - 12720 - 136
231THRTHRLYSLYS(chain C and (resid 1 through 18 or resid 20 through 127 or resid 135 through 214))CC135 - 214144 - 223
311GLUGLULEULEU(chain H and (resid 1 through 18 or resid 20 through 127 or resid 135 through 214))HE1 - 181 - 18
321LEULEUSERSER(chain H and (resid 1 through 18 or resid 20 through 127 or resid 135 through 214))HE20 - 12720 - 136
331THRTHRLYSLYS(chain H and (resid 1 through 18 or resid 20 through 127 or resid 135 through 214))HE135 - 214144 - 223
411GLUGLULEULEU(chain O and (resid 1 through 18 or resid 20 through 127 or resid 135 through 214))OG1 - 181 - 18
421LEULEUSERSER(chain O and (resid 1 through 18 or resid 20 through 127 or resid 135 through 214))OG20 - 12720 - 136
431THRTHRLYSLYS(chain O and (resid 1 through 18 or resid 20 through 127 or resid 135 through 214))OG135 - 214144 - 223
112GLUGLUCYSCYS(chain B and (resid 1 through 23 or (resid 24...BB1 - 231 - 23
122ARGARGALAALA(chain B and (resid 1 through 23 or (resid 24...BB24 - 2524 - 25
132GLUGLUGLUGLU(chain B and (resid 1 through 23 or (resid 24...BB1 - 2131 - 214
142GLUGLUGLUGLU(chain B and (resid 1 through 23 or (resid 24...BB1 - 2131 - 214
152GLUGLUGLUGLU(chain B and (resid 1 through 23 or (resid 24...BB1 - 2131 - 214
162GLUGLUGLUGLU(chain B and (resid 1 through 23 or (resid 24...BB1 - 2131 - 214
212GLUGLUGLUGLUchain DDD1 - 2131 - 214
312GLUGLUGLUGLUchain IIF1 - 2131 - 214
412GLUGLUCYSCYS(chain P and (resid 1 through 23 or (resid 24...PH1 - 231 - 23
422ARGARGALAALA(chain P and (resid 1 through 23 or (resid 24...PH24 - 2524 - 25
432GLUGLUGLUGLU(chain P and (resid 1 through 23 or (resid 24...PH1 - 2131 - 214
442GLUGLUGLUGLU(chain P and (resid 1 through 23 or (resid 24...PH1 - 2131 - 214
452GLUGLUGLUGLU(chain P and (resid 1 through 23 or (resid 24...PH1 - 2131 - 214
462GLUGLUGLUGLU(chain P and (resid 1 through 23 or (resid 24...PH1 - 2131 - 214
113VALVALSERSER(chain E and resid 2 through 112)EI2 - 1122 - 120
213VALVALSERSERchain JJJ2 - 1122 - 120
313VALVALSERSER(chain M and resid 2 through 112)MK2 - 1122 - 120
413VALVALSERSER(chain N and resid 2 through 112)NP2 - 1122 - 120
114ASNASNPROPROchain FFL1 - 121 - 12
214ASNASNPROPROchain GGM1 - 121 - 12
314ASNASNPROPROchain KKN1 - 121 - 12
414ASNASNPROPROchain LLO1 - 121 - 12

NCS ensembles :
ID
1
2
3
4

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Components

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Protein/peptide , 1 types, 4 molecules FGKL

#4: Protein/peptide
Circumsporozoite protein / / CS


Mass: 1265.286 Da / Num. of mol.: 4 / Fragment: residues 128-139 / Source method: obtained synthetically
Source: (synth.) Plasmodium falciparum (malaria parasite P. falciparum)
References: UniProt: P02893

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Antibody , 3 types, 12 molecules ACHOBDIPEJMN

#1: Antibody
4493 Fab heavy chain


Mass: 23792.730 Da / Num. of mol.: 4
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human) / Production host: Homo sapiens (human)
#2: Antibody
4493 Kappa light chain


Mass: 23460.979 Da / Num. of mol.: 4
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human) / Production host: Homo sapiens (human)
#3: Antibody
Anti-kappa VHH domain


Mass: 11326.253 Da / Num. of mol.: 4
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Lama glama (llama) / Production host: Escherichia coli (E. coli)

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Non-polymers , 3 types, 1334 molecules

#5: Chemical
ChemComp-ACT / ACETATE ION / Acetate


Mass: 59.044 Da / Num. of mol.: 8 / Source method: obtained synthetically / Formula: C2H3O2
#6: Chemical ChemComp-EDO / 1,2-ETHANEDIOL / ETHYLENE GLYCOL / Ethylene glycol


Mass: 62.068 Da / Num. of mol.: 1 / Source method: obtained synthetically / Formula: C2H6O2
#7: Water ChemComp-HOH / water / Water


Mass: 18.015 Da / Num. of mol.: 1325 / Source method: isolated from a natural source / Formula: H2O

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Details

Has ligand of interestN

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Experimental details

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Experiment

ExperimentMethod: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

CrystalDensity Matthews: 2.6 Å3/Da / Density % sol: 52.64 %
Crystal growTemperature: 298 K / Method: vapor diffusion, sitting drop / pH: 6
Details: 20 % (w/v) PEG8000, 0.1 M MES pH 6.0, 0.2 M calcium acetate

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Data collection

DiffractionMean temperature: 100 K / Serial crystal experiment: N
Diffraction sourceSource: SYNCHROTRON / Site: APS / Beamline: 23-ID-D / Wavelength: 1.0332 Å
DetectorType: DECTRIS PILATUS3 6M / Detector: PIXEL / Date: Dec 14, 2018
RadiationProtocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelengthWavelength: 1.0332 Å / Relative weight: 1
ReflectionResolution: 2.1→30 Å / Num. obs: 141733 / % possible obs: 97.7 % / Redundancy: 3.5 % / Biso Wilson estimate: 31.7 Å2 / CC1/2: 0.997 / Rmerge(I) obs: 0.069 / Rpim(I) all: 0.053 / Net I/σ(I): 9.4
Reflection shellResolution: 2.1→2.13 Å / Redundancy: 3.3 % / Rmerge(I) obs: 0.538 / Mean I/σ(I) obs: 1.7 / Num. unique obs: 5870 / CC1/2: 0.641 / Rpim(I) all: 0.349 / % possible all: 96.7

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Processing

Software
NameVersionClassification
PHENIX1.14_3260refinement
XDSdata reduction
XDSdata scaling
PHENIXphasing
RefinementMethod to determine structure: MOLECULAR REPLACEMENT
Starting model: In house model

Resolution: 2.1→29.493 Å / SU ML: 0.26 / Cross valid method: THROUGHOUT / σ(F): 1.97 / Phase error: 23.65 / Stereochemistry target values: ML
RfactorNum. reflection% reflection
Rfree0.2096 1995 1.41 %
Rwork0.1772 139707 -
obs0.1777 141702 97.7 %
Solvent computationShrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL
Displacement parametersBiso max: 138.12 Å2 / Biso mean: 44.0322 Å2 / Biso min: 18.49 Å2
Refinement stepCycle: final / Resolution: 2.1→29.493 Å
ProteinNucleic acidLigandSolventTotal
Num. atoms16283 0 36 1327 17646
Biso mean--78.31 46.35 -
Num. residues----2257
Refine LS restraints NCS
Ens-IDDom-IDAuth asym-IDNumberRefine-IDRmsType
11A3973X-RAY DIFFRACTION8.407TORSIONAL
12C3973X-RAY DIFFRACTION8.407TORSIONAL
13H3973X-RAY DIFFRACTION8.407TORSIONAL
14O3973X-RAY DIFFRACTION8.407TORSIONAL
21B4070X-RAY DIFFRACTION8.407TORSIONAL
22D4070X-RAY DIFFRACTION8.407TORSIONAL
23I4070X-RAY DIFFRACTION8.407TORSIONAL
24P4070X-RAY DIFFRACTION8.407TORSIONAL
31E2235X-RAY DIFFRACTION8.407TORSIONAL
32J2235X-RAY DIFFRACTION8.407TORSIONAL
33M2235X-RAY DIFFRACTION8.407TORSIONAL
34N2235X-RAY DIFFRACTION8.407TORSIONAL
41F216X-RAY DIFFRACTION8.407TORSIONAL
42G216X-RAY DIFFRACTION8.407TORSIONAL
43K216X-RAY DIFFRACTION8.407TORSIONAL
44L216X-RAY DIFFRACTION8.407TORSIONAL
LS refinement shell

Refine-ID: X-RAY DIFFRACTION / Rfactor Rfree error: 0

Resolution (Å)Rfactor RfreeNum. reflection RfreeRfactor RworkNum. reflection Rwork% reflection obs (%)
2.1-2.15250.34881200.2892986497
2.1525-2.21070.2961580.264987397
2.2107-2.27570.29631340.251988697
2.2757-2.34920.24621470.2361996997
2.3492-2.43310.29031460.2275991897
2.4331-2.53040.25871420.2163999697
2.5304-2.64550.22221460.2099992098
2.6455-2.78490.25081380.20011002198
2.7849-2.95920.25551420.19241003498
2.9592-3.18750.20971400.18581000398
3.1875-3.50780.19081450.16921002298
3.5078-4.01430.19231470.15171005998
4.0143-5.05340.15091450.1261006699
5.0534-29.4930.1761450.15291007698
Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

IDL11 (°2)L12 (°2)L13 (°2)L22 (°2)L23 (°2)L33 (°2)S11 (Å °)S12 (Å °)S13 (Å °)S21 (Å °)S22 (Å °)S23 (Å °)S31 (Å °)S32 (Å °)S33 (Å °)T11 (Å2)T12 (Å2)T13 (Å2)T22 (Å2)T23 (Å2)T33 (Å2)Origin x (Å)Origin y (Å)Origin z (Å)
11.63-1.0835-0.56553.24121.27681.7367-0.00040.0532-0.06310.09230.02990.0640.0569-0.0903-0.02780.3405-0.0250.06070.242-0.02330.2796-54.3003-13.65955.454
24.7204-0.21171.49843.3212-0.38762.0339-0.2586-0.3386-0.1540.48380.0548-0.4076-0.11350.08280.18490.37240.1278-0.08270.3453-0.03480.3304-19.2834-24.646955.6378
35.38770.17670.61091.7108-0.56441.5114-0.1769-0.04220.27870.1870.0937-0.1257-0.20910.06190.1130.4486-0.01780.02140.2306-0.04850.3223-41.99995.067756.9995
41.9081-1.9535-1.58085.77582.34183.0146-0.07190.10470.21680.0570.0801-0.58850.150.2469-0.01320.23160.0554-0.05330.3765-0.0010.402-15.669-13.818943.7079
52.3555-0.7098-1.60442.09891.39712.3634-0.06-0.0098-0.1276-0.05540.0924-0.0870.13710.0764-0.02390.2355-0.0260.03080.2353-0.03280.2426-41.7312-58.921210.8671
64.8657-0.41491.99583.04391.09824.14950.1415-0.156-0.41060.4005-0.1283-0.2180.42570.1723-0.00060.3088-0.009-0.03250.28740.07760.3578-6.6371-69.85654.0385
75.84650.4404-0.12030.8994-0.35611.13620.0331-0.30120.6263-0.00760.0336-0.1474-0.20950.2018-0.05060.3477-0.0750.08890.3007-0.12440.431-28.8129-40.809113.502
81.87570.5594-0.21354.39072.64113.99920.02320.0447-0.1729-0.01040.0193-0.20140.18250.0856-0.05180.1966-0.0063-0.02550.32850.01990.321-4.506-57.0889-5.8924
91.0905-0.2031-0.77082.1641.32875.30980.02880.0020.0673-0.1051-0.0121-0.0932-0.0463-0.0666-0.01940.2247-0.01630.03670.2237-0.02340.2595-9.4022.274-8.3656
102.05710.59410.21954.7057-0.23144.2796-0.00860.0325-0.26870.15830.01430.06140.2585-0.1245-0.03780.2567-0.03730.07840.2531-0.04140.2936-25.9593-13.148720.8788
112.66531.9252-1.00545.0139-1.61432.8840.00140.07450.0305-0.47890.00320.34950.4076-0.51540.00240.3969-0.0912-0.05070.4792-0.06760.2969-25.9109-10.3919-16.6545
122.11560.50490.50263.48682.35743.5449-0.11640.04830.0743-0.01380.07450.1884-0.1035-0.26270.04840.32-0.06810.08640.39480.01430.4111-40.9994-9.474415.1348
131.2704-0.3927-0.68352.30111.54963.690.0171-0.02960.09140.05580.0499-0.0685-0.040.0141-0.07320.2143-0.00110.01050.2257-0.02250.265322.6848-38.976738.417
142.30682.2448-0.32826.238-0.90294.22670.0952-0.1447-0.1560.1399-0.0207-0.00660.14010.1274-0.08760.23450.0056-0.01740.3138-0.00850.23234.0836-51.065664.4432
153.2081.462-1.72073.2715-1.18382.5938-0.21270.3009-0.0702-0.30630.19320.08240.377-0.51820.00780.3003-0.0524-0.01730.3726-0.04520.25126.2775-50.661228.2546
161.32360.1616-0.30382.9512.42475.3955-0.0133-0.06450.07720.0370.04580.1096-0.1557-0.4433-0.0170.21910.00980.00490.36450.0330.2194-11.146-47.472259.8006
175.10481.40920.99755.7661.25733.6755-0.12410.3122-0.0373-0.29610.09750.1227-0.2187-0.02060.01270.3211-0.1006-0.00560.4770.04670.3984-55.5531-22.5271-6.3114
183.2161.04780.0874.71110.35111.55020.0382-0.1078-0.2041-0.2469-0.1940.6060.2495-0.80020.11330.4724-0.2243-0.07470.9076-0.03060.4444-26.4386-59.750138.7814
196.16392.35590.18354.24220.59113.59580.03410.12560.49930.0375-0.00540.2503-0.3667-0.0227-0.02170.2846-0.02120.020.32790.01550.36470.4131-29.54331.0064
208.1454-0.44651.57942.00481.8567.8091-0.02011.52750.492-1.14050.1717-0.1668-0.7116-0.2835-0.17620.4963-0.01920.04010.47230.10370.4235-64.83081.067850.3297
218.4651.6468-0.77166.80182.32149.38280.02751.02181.1415-0.65020.28460.1681-0.7413-0.5219-0.29770.30640.0403-0.00060.37590.05520.3952-52.47-43.61429.2893
228.25410.5279-1.74279.17491.19227.3183-0.20830.79461.1745-0.35710.19540.1468-1.4436-0.6726-0.0190.56680.08380.00930.27830.09120.3542-13.22158.2467-25.584
232.26-2.15661.42052.2861-0.27076.3360.15180.41460.6286-0.5153-0.08930.0138-0.4775-0.3029-0.08850.3523-0.02210.03570.25790.01820.366723.6136-36.127619.9658
244.31172.3134-0.82234.85340.11534.02430.0750.1780.3041-0.06370.1583-0.2766-0.42980.3902-0.22210.4054-0.0220.02140.4970.04750.6078-13.119914.508244.5037
Refinement TLS group
IDRefine-IDRefine TLS-IDSelection detailsAuth asym-IDAuth seq-ID
1X-RAY DIFFRACTION1chain 'A' and (resid 1 through 113 )A1 - 113
2X-RAY DIFFRACTION2chain 'A' and (resid 114 through 215 )A114 - 215
3X-RAY DIFFRACTION3chain 'B' and (resid 1 through 107 )B1 - 107
4X-RAY DIFFRACTION4chain 'B' and (resid 108 through 213 )B108 - 213
5X-RAY DIFFRACTION5chain 'C' and (resid 1 through 113 )C1 - 113
6X-RAY DIFFRACTION6chain 'C' and (resid 114 through 214 )C114 - 214
7X-RAY DIFFRACTION7chain 'D' and (resid 1 through 107 )D1 - 107
8X-RAY DIFFRACTION8chain 'D' and (resid 108 through 213 )D108 - 213
9X-RAY DIFFRACTION9chain 'H' and (resid 1 through 113 )H1 - 113
10X-RAY DIFFRACTION10chain 'H' and (resid 114 through 214 )H114 - 214
11X-RAY DIFFRACTION11chain 'I' and (resid 1 through 107 )I1 - 107
12X-RAY DIFFRACTION12chain 'I' and (resid 108 through 213 )I108 - 213
13X-RAY DIFFRACTION13chain 'O' and (resid 1 through 113 )O1 - 113
14X-RAY DIFFRACTION14chain 'O' and (resid 114 through 214 )O114 - 214
15X-RAY DIFFRACTION15chain 'P' and (resid 1 through 107 )P1 - 107
16X-RAY DIFFRACTION16chain 'P' and (resid 108 through 213 )P108 - 213
17X-RAY DIFFRACTION17chain 'E' and (resid 2 through 113 )E2 - 113
18X-RAY DIFFRACTION18chain 'J' and (resid 2 through 112 )J2 - 112
19X-RAY DIFFRACTION19chain 'M' and (resid 1 through 113 )M1 - 113
20X-RAY DIFFRACTION20chain 'F' and (resid 1 through 12 )F1 - 12
21X-RAY DIFFRACTION21chain 'G' and (resid 1 through 12 )G1 - 12
22X-RAY DIFFRACTION22chain 'K' and (resid 1 through 12 )K1 - 12
23X-RAY DIFFRACTION23chain 'L' and (resid 1 through 12 )L1 - 12
24X-RAY DIFFRACTION24chain 'N' and (resid 1 through 112 )N1 - 112

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