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- PDB-6o2a: Crystal structure of 4493 Fab in complex with circumsporozoite pr... -

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Basic information

Entry
Database: PDB / ID: 6o2a
TitleCrystal structure of 4493 Fab in complex with circumsporozoite protein NDN and anti-kappa VHH domain
Components
  • 4493 Fab heavy chain
  • 4493 Kappa light chain
  • Anti-kappa VHH domain
  • Circumsporozoite protein
KeywordsIMMUNE SYSTEM / Malaria / antibody
Function / homology
Function and homology information


entry into host cell by a symbiont-containing vacuole / side of membrane / cell surface / plasma membrane / cytoplasm
Similarity search - Function
Plasmodium circumsporozoite protein / Thrombospondin type 1 domain / Thrombospondin type-1 (TSP1) repeat superfamily / Thrombospondin type-1 (TSP1) repeat profile. / Thrombospondin type 1 repeats / Thrombospondin type-1 (TSP1) repeat
Similarity search - Domain/homology
Circumsporozoite protein
Similarity search - Component
Biological speciesHomo sapiens (human)
Lama glama (llama)
Plasmodium falciparum (malaria parasite P. falciparum)
MethodX-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 2.15 Å
AuthorsScally, S.W. / Bosch, A. / Prieto, K. / Murugan, R. / Wardemann, H. / Julien, J.P.
Funding support United States, 1items
OrganizationGrant numberCountry
Bill & Melinda Gates Foundation United States
CitationJournal: Nat. Med. / Year: 2020
Title: Evolution of protective human antibodies against Plasmodium falciparum circumsporozoite protein repeat motifs.
Authors: Murugan, R. / Scally, S.W. / Costa, G. / Mustafa, G. / Thai, E. / Decker, T. / Bosch, A. / Prieto, K. / Levashina, E.A. / Julien, J.P. / Wardemann, H.
History
DepositionFeb 22, 2019Deposition site: RCSB / Processing site: RCSB
Revision 1.0Jul 1, 2020Provider: repository / Type: Initial release
Revision 1.1Jul 29, 2020Group: Database references / Category: citation / citation_author
Item: _citation.journal_volume / _citation.page_first ..._citation.journal_volume / _citation.page_first / _citation.page_last / _citation_author.identifier_ORCID
Revision 1.2Apr 3, 2024Group: Data collection / Database references / Refinement description
Category: chem_comp_atom / chem_comp_bond ...chem_comp_atom / chem_comp_bond / database_2 / pdbx_initial_refinement_model / struct_ncs_dom_lim
Item: _database_2.pdbx_DOI / _database_2.pdbx_database_accession ..._database_2.pdbx_DOI / _database_2.pdbx_database_accession / _struct_ncs_dom_lim.beg_auth_comp_id / _struct_ncs_dom_lim.beg_label_asym_id / _struct_ncs_dom_lim.beg_label_comp_id / _struct_ncs_dom_lim.beg_label_seq_id / _struct_ncs_dom_lim.end_auth_comp_id / _struct_ncs_dom_lim.end_label_asym_id / _struct_ncs_dom_lim.end_label_comp_id / _struct_ncs_dom_lim.end_label_seq_id

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Assembly

Deposited unit
A: 4493 Fab heavy chain
B: 4493 Kappa light chain
C: 4493 Fab heavy chain
D: 4493 Kappa light chain
H: 4493 Fab heavy chain
I: 4493 Kappa light chain
O: 4493 Fab heavy chain
P: 4493 Kappa light chain
E: Anti-kappa VHH domain
F: Circumsporozoite protein
G: Circumsporozoite protein
K: Circumsporozoite protein
L: Circumsporozoite protein
M: Anti-kappa VHH domain
N: Anti-kappa VHH domain
J: Anti-kappa VHH domain
hetero molecules


Theoretical massNumber of molelcules
Total (without water)240,38234
Polymers239,26516
Non-polymers1,11718
Water12,268681
1
A: 4493 Fab heavy chain
B: 4493 Kappa light chain
E: Anti-kappa VHH domain
F: Circumsporozoite protein
hetero molecules


Theoretical massNumber of molelcules
Total (without water)60,18910
Polymers59,8164
Non-polymers3726
Water724
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
2
C: 4493 Fab heavy chain
D: 4493 Kappa light chain
G: Circumsporozoite protein
M: Anti-kappa VHH domain
hetero molecules


Theoretical massNumber of molelcules
Total (without water)60,18910
Polymers59,8164
Non-polymers3726
Water724
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
3
H: 4493 Fab heavy chain
I: 4493 Kappa light chain
K: Circumsporozoite protein
N: Anti-kappa VHH domain
hetero molecules


Theoretical massNumber of molelcules
Total (without water)60,0027
Polymers59,8164
Non-polymers1863
Water724
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
4
O: 4493 Fab heavy chain
P: 4493 Kappa light chain
L: Circumsporozoite protein
J: Anti-kappa VHH domain
hetero molecules


Theoretical massNumber of molelcules
Total (without water)60,0027
Polymers59,8164
Non-polymers1863
Water724
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Unit cell
Length a, b, c (Å)82.654, 90.728, 92.515
Angle α, β, γ (deg.)89.380, 64.290, 76.880
Int Tables number1
Space group name H-MP1
Noncrystallographic symmetry (NCS)NCS domain:
IDEns-IDDetails
11(chain A and (resid 1 through 18 or resid 20...
21(chain C and (resid 1 through 12 or (resid 13...
31(chain H and (resid 1 through 12 or (resid 13...
41(chain O and (resid 1 through 12 or (resid 13...
12(chain B and (resid 1 through 23 or (resid 24...
22(chain D and (resid 1 through 23 or (resid 24...
32(chain I and (resid 1 through 53 or (resid 54...
42(chain P and resid 1 through 213)
13(chain E and resid 2 through 112)
23chain J
33(chain M and resid 2 through 112)
43(chain N and resid 2 through 112)
14chain F
24chain G
34chain K
44chain L

NCS domain segments:
Dom-IDComponent-IDEns-IDBeg auth comp-IDBeg label comp-IDEnd auth comp-IDEnd label comp-IDSelection detailsAuth asym-IDLabel asym-IDAuth seq-IDLabel seq-ID
111GLUGLULEULEU(chain A and (resid 1 through 18 or resid 20...AA1 - 181 - 18
121LEULEUVALVAL(chain A and (resid 1 through 18 or resid 20...AA20 - 6320 - 66
131LYSLYSLYSLYS(chain A and (resid 1 through 18 or resid 20...AA6467
141GLUGLUCYSCYS(chain A and (resid 1 through 18 or resid 20...AA1 - 2161 - 225
151GLUGLUCYSCYS(chain A and (resid 1 through 18 or resid 20...AA1 - 2161 - 225
161GLUGLUCYSCYS(chain A and (resid 1 through 18 or resid 20...AA1 - 2161 - 225
171GLUGLUCYSCYS(chain A and (resid 1 through 18 or resid 20...AA1 - 2161 - 225
211GLUGLUVALVAL(chain C and (resid 1 through 12 or (resid 13...CC1 - 121 - 12
221GLNGLNGLNGLN(chain C and (resid 1 through 12 or (resid 13...CC1313
231GLUGLULYSLYS(chain C and (resid 1 through 12 or (resid 13...CC1 - 2141 - 223
241GLUGLULYSLYS(chain C and (resid 1 through 12 or (resid 13...CC1 - 2141 - 223
251GLUGLULYSLYS(chain C and (resid 1 through 12 or (resid 13...CC1 - 2141 - 223
261GLUGLULYSLYS(chain C and (resid 1 through 12 or (resid 13...CC1 - 2141 - 223
311GLUGLUVALVAL(chain H and (resid 1 through 12 or (resid 13...HE1 - 121 - 12
321GLNGLNGLNGLN(chain H and (resid 1 through 12 or (resid 13...HE1313
331GLUGLUSERSER(chain H and (resid 1 through 12 or (resid 13...HE1 - 2151 - 224
341GLUGLUSERSER(chain H and (resid 1 through 12 or (resid 13...HE1 - 2151 - 224
351GLUGLUSERSER(chain H and (resid 1 through 12 or (resid 13...HE1 - 2151 - 224
361GLUGLUSERSER(chain H and (resid 1 through 12 or (resid 13...HE1 - 2151 - 224
411GLUGLUVALVAL(chain O and (resid 1 through 12 or (resid 13...OG1 - 121 - 12
421GLNGLNGLNGLN(chain O and (resid 1 through 12 or (resid 13...OG1313
431GLUGLUSERSER(chain O and (resid 1 through 12 or (resid 13...OG1 - 2151 - 224
441GLUGLUSERSER(chain O and (resid 1 through 12 or (resid 13...OG1 - 2151 - 224
451GLUGLUSERSER(chain O and (resid 1 through 12 or (resid 13...OG1 - 2151 - 224
112GLUGLUCYSCYS(chain B and (resid 1 through 23 or (resid 24...BB1 - 231 - 23
122ARGARGALAALA(chain B and (resid 1 through 23 or (resid 24...BB24 - 2524 - 25
132GLUGLUCYSCYS(chain B and (resid 1 through 23 or (resid 24...BB1 - 2141 - 215
142GLUGLUCYSCYS(chain B and (resid 1 through 23 or (resid 24...BB1 - 2141 - 215
152GLUGLUCYSCYS(chain B and (resid 1 through 23 or (resid 24...BB1 - 2141 - 215
162GLUGLUCYSCYS(chain B and (resid 1 through 23 or (resid 24...BB1 - 2141 - 215
212GLUGLUCYSCYS(chain D and (resid 1 through 23 or (resid 24...DD1 - 231 - 23
222ARGARGALAALA(chain D and (resid 1 through 23 or (resid 24...DD24 - 2524 - 25
232GLUGLUGLUGLU(chain D and (resid 1 through 23 or (resid 24...DD1 - 2131 - 214
242GLUGLUGLUGLU(chain D and (resid 1 through 23 or (resid 24...DD1 - 2131 - 214
252GLUGLUGLUGLU(chain D and (resid 1 through 23 or (resid 24...DD1 - 2131 - 214
262GLUGLUGLUGLU(chain D and (resid 1 through 23 or (resid 24...DD1 - 2131 - 214
312GLUGLUSERSER(chain I and (resid 1 through 53 or (resid 54...IF1 - 531 - 54
322ARGARGALAALA(chain I and (resid 1 through 53 or (resid 54...IF54 - 5555 - 56
332GLUGLUGLUGLU(chain I and (resid 1 through 53 or (resid 54...IF1 - 2131 - 214
342GLUGLUGLUGLU(chain I and (resid 1 through 53 or (resid 54...IF1 - 2131 - 214
352GLUGLUGLUGLU(chain I and (resid 1 through 53 or (resid 54...IF1 - 2131 - 214
362GLUGLUGLUGLU(chain I and (resid 1 through 53 or (resid 54...IF1 - 2131 - 214
412GLUGLUGLUGLU(chain P and resid 1 through 213)PH1 - 2131 - 214
113VALVALSERSER(chain E and resid 2 through 112)EI2 - 1122 - 120
213VALVALSERSERchain JJP2 - 1122 - 120
313VALVALSERSER(chain M and resid 2 through 112)MN2 - 1122 - 120
413VALVALSERSER(chain N and resid 2 through 112)NO2 - 1122 - 120
114ASNASNASNASNchain FFJ1 - 91 - 9
214ASNASNASNASNchain GGK1 - 91 - 9
314ASNASNASNASNchain KKL1 - 91 - 9
414ASNASNASNASNchain LLM1 - 91 - 9

NCS ensembles :
ID
1
2
3
4

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Components

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Protein/peptide , 1 types, 4 molecules FGKL

#4: Protein/peptide
Circumsporozoite protein / / CS


Mass: 1236.248 Da / Num. of mol.: 4 / Fragment: residues 124-135 / Source method: obtained synthetically
Source: (synth.) Plasmodium falciparum (malaria parasite P. falciparum)
References: UniProt: P02893

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Antibody , 3 types, 12 molecules ACHOBDIPEMNJ

#1: Antibody
4493 Fab heavy chain


Mass: 23792.730 Da / Num. of mol.: 4
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human) / Production host: Homo sapiens (human)
#2: Antibody
4493 Kappa light chain


Mass: 23460.979 Da / Num. of mol.: 4
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human) / Production host: Homo sapiens (human)
#3: Antibody
Anti-kappa VHH domain


Mass: 11326.253 Da / Num. of mol.: 4
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Lama glama (llama) / Production host: Escherichia coli (E. coli)

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Non-polymers , 2 types, 699 molecules

#5: Chemical
ChemComp-EDO / 1,2-ETHANEDIOL / ETHYLENE GLYCOL / Ethylene glycol


Mass: 62.068 Da / Num. of mol.: 18 / Source method: obtained synthetically / Formula: C2H6O2
#6: Water ChemComp-HOH / water / Water


Mass: 18.015 Da / Num. of mol.: 681 / Source method: isolated from a natural source / Formula: H2O

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Details

Has ligand of interestN

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Experimental details

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Experiment

ExperimentMethod: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

CrystalDensity Matthews: 2.45 Å3/Da / Density % sol: 49.85 %
Crystal growTemperature: 298 K / Method: vapor diffusion, sitting drop / Details: 20 % (w/v) PEG3350, 0.2 M potassium nitrate

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Data collection

DiffractionMean temperature: 100 K / Serial crystal experiment: N
Diffraction sourceSource: SYNCHROTRON / Site: APS / Beamline: 23-ID-D / Wavelength: 1.0332 Å
DetectorType: DECTRIS PILATUS3 6M / Detector: PIXEL / Date: Dec 14, 2018
RadiationProtocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelengthWavelength: 1.0332 Å / Relative weight: 1
ReflectionResolution: 2.15→30 Å / Num. obs: 124730 / % possible obs: 97.7 % / Redundancy: 3.5 % / Biso Wilson estimate: 39.7 Å2 / CC1/2: 0.997 / Rmerge(I) obs: 0.07 / Rpim(I) all: 0.044 / Net I/σ(I): 10.5
Reflection shellResolution: 2.15→2.18 Å / Redundancy: 3.5 % / Rmerge(I) obs: 0.544 / Mean I/σ(I) obs: 1.7 / Num. unique obs: 5069 / CC1/2: 0.722 / Rpim(I) all: 0.339 / % possible all: 96.9

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Processing

Software
NameVersionClassification
PHENIX1.14_3260refinement
XDSdata reduction
XDSdata scaling
PHENIXphasing
RefinementMethod to determine structure: MOLECULAR REPLACEMENT
Starting model: In house model

Resolution: 2.15→29.415 Å / SU ML: 0.27 / Cross valid method: THROUGHOUT / σ(F): 2 / Phase error: 27.66 / Stereochemistry target values: ML
RfactorNum. reflection% reflection
Rfree0.2211 1357 1.09 %
Rwork0.1928 123305 -
obs0.1932 124662 97.68 %
Solvent computationShrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL
Displacement parametersBiso max: 148.93 Å2 / Biso mean: 54.1797 Å2 / Biso min: 24.25 Å2
Refinement stepCycle: final / Resolution: 2.15→29.415 Å
ProteinNucleic acidLigandSolventTotal
Num. atoms16260 0 72 681 17013
Biso mean--65.46 51.67 -
Num. residues----2259
Refine LS restraints NCS
Ens-IDDom-IDAuth asym-IDNumberRefine-IDRmsType
11A3989X-RAY DIFFRACTION8.971TORSIONAL
12C3989X-RAY DIFFRACTION8.971TORSIONAL
13H3989X-RAY DIFFRACTION8.971TORSIONAL
14O3989X-RAY DIFFRACTION8.971TORSIONAL
21B4077X-RAY DIFFRACTION8.971TORSIONAL
22D4077X-RAY DIFFRACTION8.971TORSIONAL
23I4077X-RAY DIFFRACTION8.971TORSIONAL
24P4077X-RAY DIFFRACTION8.971TORSIONAL
31E2247X-RAY DIFFRACTION8.971TORSIONAL
32J2247X-RAY DIFFRACTION8.971TORSIONAL
33M2247X-RAY DIFFRACTION8.971TORSIONAL
34N2247X-RAY DIFFRACTION8.971TORSIONAL
41F162X-RAY DIFFRACTION8.971TORSIONAL
42G162X-RAY DIFFRACTION8.971TORSIONAL
43K162X-RAY DIFFRACTION8.971TORSIONAL
44L162X-RAY DIFFRACTION8.971TORSIONAL
LS refinement shell

Refine-ID: X-RAY DIFFRACTION / Rfactor Rfree error: 0

Resolution (Å)Rfactor RfreeNum. reflection RfreeRfactor RworkNum. reflection Rwork% reflection obs (%)
2.15-2.22680.31451360.31121223197
2.2268-2.31590.34671330.2951220197
2.3159-2.42130.32641350.28391230097
2.4213-2.54890.3051360.25791227797
2.5489-2.70850.28421350.2331232498
2.7085-2.91740.24911370.22281241598
2.9174-3.21070.25711350.21211233998
3.2107-3.67450.20251370.18611238298
3.6745-4.62660.17511360.14911243198
4.6266-29.4150.17091370.15131240598
Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

IDL11 (°2)L12 (°2)L13 (°2)L22 (°2)L23 (°2)L33 (°2)S11 (Å °)S12 (Å °)S13 (Å °)S21 (Å °)S22 (Å °)S23 (Å °)S31 (Å °)S32 (Å °)S33 (Å °)T11 (Å2)T12 (Å2)T13 (Å2)T22 (Å2)T23 (Å2)T33 (Å2)Origin x (Å)Origin y (Å)Origin z (Å)
12.0323-0.42380.1542.5283-1.23182.5138-0.1068-0.05090.01620.11940.0790.1884-0.0421-0.21250.03420.30710.09360.0240.43130.00820.2504-51.793351.787811.3828
22.65470.8926-0.69572.193-0.29333.4955-0.1392-0.33640.14970.06620.0983-0.1287-0.40450.21020.04470.42990.0217-0.08770.35780.01410.2611-15.822450.728423.802
33.96820.5711-0.55851.6672-0.13520.4402-0.07190.39980.0893-0.28450.03510.1167-0.0538-0.11570.06130.40120.061-0.03210.45750.04660.2338-38.59154.443-6.3299
41.318-0.5728-0.11912.7835-1.66792.7125-0.0816-0.1512-0.17150.23490.0298-0.108-0.09830.14010.05050.2912-0.0417-0.04030.34580.01670.2652-12.792740.046712.71
53.4306-0.1441-0.35621.6464-1.19342.3690.26840.30310.25530.1404-0.18150.2042-0.3014-0.1668-0.07670.47280.12050.13360.38130.03630.3556-39.65584.439254.1878
63.11030.56950.17241.9577-0.46030.99740.0925-0.3170.3501-0.07720.3408-0.4279-0.89871.0911-0.31210.5545-0.27220.04890.7421-0.15950.4298-5.3131.807464.1359
74.9541.073-1.18771.60680.37732.90730.01570.63280.1639-0.2050.05770.054-0.4034-0.2089-0.04410.58170.15560.07710.5520.10020.318-27.50137.156535.3829
81.9281-0.6973-0.87882.5423-1.60813.3799-0.1037-0.19620.02720.1310.1871-0.2792-0.23560.4492-0.03240.3511-0.0503-0.03860.5495-0.12190.4104-1.8932-9.147552.6206
91.29890.1129-0.43594.8117-1.6383.08590.170.0259-0.09730.0372-0.19290.0194-0.04780.3841-0.00070.20190.00480.00460.3539-0.00010.2685-5.2628-7.8196-6.9617
102.6894-0.18980.2853.3428-0.16384.1411-0.0209-0.18520.03740.0626-0.08160.0076-0.08450.09420.10320.3291-0.03770.07980.2362-0.01620.313-23.675918.36749.6605
113.0993-0.6531-1.33821.7334-0.38013.74820.008-0.0116-0.11540.3285-0.02510.50830.3589-0.13150.02420.402-0.08350.050.2606-0.01710.5402-22.6964-17.96032.7572
122.14460.3666-0.04614.4644-1.53371.6174-0.15880.1826-0.1451-0.2586-0.0580.23190.1329-0.09620.22330.4136-0.0520.08410.3686-0.09750.3752-38.349913.39854.5048
134.4601-0.3618-1.76423.0389-0.48642.271-0.02870.4644-0.8516-0.033-0.1010.11210.0128-0.05580.12540.25640.00090.00520.3009-0.03660.418424.506932.874135.2808
143.5862-0.88330.50834.3241-0.20392.99290.067-0.22830.2978-0.0409-0.012-0.2736-0.05640.0894-0.03390.34170.00660.03290.22870.03580.24726.752457.863150.5222
152.6493-0.6921-0.85740.58630.28970.5956-0.7765-0.0149-2.93460.348-0.12840.46351.2147-0.6283-0.56230.4404-0.33480.2861-0.06450.05881.37068.701422.182347.0847
162.19640.3147-0.30674.2148-1.90492.29270.105-0.00580.1603-0.1087-0.19840.2288-0.2186-0.14650.12520.4090.004-0.02840.3201-0.03210.255-8.65653.49747.124
173.5348-1.7188-1.48373.2581-0.23053.64730.29530.4162-0.0973-0.2642-0.4625-0.39450.03290.14890.18540.25780.0064-0.01510.39610.02710.3272-8.596543.5777-15.5287
182.00850.3665-1.33816.161-0.09157.3707-0.0302-0.3888-0.0621-0.1535-0.4310.73451.0454-1.05480.44180.5524-0.10030.01050.8647-0.00760.5274-63.551249.29-1.6053
198.3792-1.0779-4.29866.60561.57727.0288-0.03710.55870.4811-0.0586-0.35590.42080.1471-1.44270.43090.53970.2487-0.01971.06190.0540.5693-52.38444.309141.4607
203.5322-0.6686-3.47467.56670.55673.5161-1.25690.5067-0.2874-0.380.4171-0.44791.16760.58850.80870.55240.0430.12470.6718-0.11490.6103-4.2311-23.9219-14.2221
210.30090.56660.3261.54580.32150.5232-0.11270.2175-0.2486-0.43410.27280.0583-0.05890.1470.08090.5379-0.20660.13450.4332-0.42651.4927.86615.748431.1643
225.347-0.3573-2.95593.2155-0.83134.75210.05260.8624-0.2085-0.6414-0.1878-0.19230.1166-0.45160.13070.5016-0.01570.090.7561-0.10470.52921.4046-5.292124.3871
235.16440.4493-1.22772.6954-0.77314.1919-0.0453-0.1631-0.5005-0.03010.05490.44430.2769-0.4429-0.02590.4994-0.06750.11110.488-0.0410.7341-53.8633-8.900414.479
246.1210.7901-1.21673.749-0.91265.1838-0.07970.0021-0.16530.0604-0.10310.2649-0.1057-0.5160.16650.3151-0.01530.00410.3977-0.040.3712-23.623131.26657.8292
Refinement TLS group
IDRefine-IDRefine TLS-IDSelection detailsAuth asym-IDAuth seq-ID
1X-RAY DIFFRACTION1chain 'A' and (resid 1 through 113 )A1 - 113
2X-RAY DIFFRACTION2chain 'A' and (resid 114 through 216 )A114 - 216
3X-RAY DIFFRACTION3chain 'B' and (resid 1 through 107 )B1 - 107
4X-RAY DIFFRACTION4chain 'B' and (resid 108 through 214 )B108 - 214
5X-RAY DIFFRACTION5chain 'C' and (resid 1 through 113 )C1 - 113
6X-RAY DIFFRACTION6chain 'C' and (resid 114 through 214 )C114 - 214
7X-RAY DIFFRACTION7chain 'D' and (resid 1 through 107 )D1 - 107
8X-RAY DIFFRACTION8chain 'D' and (resid 108 through 213 )D108 - 213
9X-RAY DIFFRACTION9chain 'H' and (resid 1 through 113 )H1 - 113
10X-RAY DIFFRACTION10chain 'H' and (resid 114 through 215 )H114 - 215
11X-RAY DIFFRACTION11chain 'I' and (resid 1 through 107 )I1 - 107
12X-RAY DIFFRACTION12chain 'I' and (resid 108 through 213 )I108 - 213
13X-RAY DIFFRACTION13chain 'O' and (resid 1 through 113 )O1 - 113
14X-RAY DIFFRACTION14chain 'O' and (resid 114 through 215 )O114 - 215
15X-RAY DIFFRACTION15chain 'P' and (resid 1 through 107 )P1 - 107
16X-RAY DIFFRACTION16chain 'P' and (resid 108 through 213 )P108 - 213
17X-RAY DIFFRACTION17chain 'E' and (resid 1 through 113 )E1 - 113
18X-RAY DIFFRACTION18chain 'F' and (resid 1 through 9 )F1 - 9
19X-RAY DIFFRACTION19chain 'G' and (resid 1 through 9 )G1 - 9
20X-RAY DIFFRACTION20chain 'K' and (resid 1 through 9 )K1 - 9
21X-RAY DIFFRACTION21chain 'L' and (resid 1 through 9 )L1 - 9
22X-RAY DIFFRACTION22chain 'M' and (resid 1 through 112 )M1 - 112
23X-RAY DIFFRACTION23chain 'N' and (resid 2 through 112 )N2 - 112
24X-RAY DIFFRACTION24chain 'J' and (resid 2 through 112 )J2 - 112

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