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Open data
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Basic information
Entry | Database: PDB / ID: 6ny9 | ||||||
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Title | Alpha/beta hydrolase domain-containing protein 10 from mouse | ||||||
![]() | Mycophenolic acid acyl-glucuronide esterase, mitochondrial | ||||||
![]() | HYDROLASE / Alpha/beta hydrolase / Depalmitoylase / Mitochondria | ||||||
Function / homology | ![]() mycophenolic acid acyl-glucuronide esterase / mycophenolic acid acyl-glucuronide esterase activity / Glucuronidation / protein depalmitoylation / palmitoyl[protein] hydrolase / palmitoyl-(protein) hydrolase activity / glucuronoside catabolic process / hydrolase activity, hydrolyzing O-glycosyl compounds / mitochondrion / cytosol Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Cao, Y. / Rice, P.A. / Dickinson, B.C. | ||||||
Funding support | ![]()
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![]() | ![]() Title: ABHD10 is an S-depalmitoylase affecting redox homeostasis through peroxiredoxin-5. Authors: Cao, Y. / Qiu, T. / Kathayat, R.S. / Azizi, S.A. / Thorne, A.K. / Ahn, D. / Fukata, Y. / Fukata, M. / Rice, P.A. / Dickinson, B.C. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 194.1 KB | Display | ![]() |
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PDB format | ![]() | 131.6 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 438.9 KB | Display | ![]() |
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Full document | ![]() | 439.6 KB | Display | |
Data in XML | ![]() | 13.1 KB | Display | |
Data in CIF | ![]() | 18.8 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 3llcS S: Starting model for refinement |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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1 | ![]()
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Unit cell |
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Components on special symmetry positions |
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Components
#1: Protein | Mass: 27821.311 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() References: UniProt: Q6PE15, mycophenolic acid acyl-glucuronide esterase | ||
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#2: Chemical | ChemComp-MPD / ( | ||
#3: Chemical | ChemComp-SR / #4: Water | ChemComp-HOH / | |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 3.83 Å3/Da / Density % sol: 67.91 % |
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Crystal grow | Temperature: 277.15 K / Method: liquid diffusion / pH: 6.4 Details: 0.08 M strontium chloride hexahydrate, 0.04 M sodium cacodylate trihydrate pH 6.4, 25% v/v (+/-)-2-methyl-2,4-pentanediol (MPD), 0.012 M spermine tetrahydrochloride PH range: 6.0-6.4 |
-Data collection
Diffraction | Mean temperature: 100 K / Serial crystal experiment: N |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: DECTRIS PILATUS 6M-F / Detector: PIXEL / Date: Nov 14, 2017 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.979 Å / Relative weight: 1 |
Reflection | Resolution: 1.657→56.46 Å / Num. obs: 52270 / % possible obs: 92.82 % / Redundancy: 12.9 % / Biso Wilson estimate: 16.57 Å2 / CC1/2: 0.998 / Rmerge(I) obs: 0.1082 / Net I/σ(I): 20.82 |
Reflection shell | Resolution: 1.657→1.717 Å / Redundancy: 12.8 % / Mean I/σ(I) obs: 1.6 / Num. unique obs: 4994 / CC1/2: 0.686 / % possible all: 76.98 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: 3LLC Resolution: 1.66→56.46 Å / SU ML: 0.1917 / Cross valid method: FREE R-VALUE / σ(F): 0 / Phase error: 22.0141
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 24.19 Å2 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 1.66→56.46 Å
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Refine LS restraints |
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LS refinement shell |
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Refinement TLS params. | Method: refined / Origin x: 29.4150756586 Å / Origin y: -34.2369923944 Å / Origin z: 17.0271591659 Å
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Refinement TLS group | Selection details: all |