response to methotrexate / dihydrofolate reductase / dihydrofolate reductase activity / tetrahydrofolate biosynthetic process / one-carbon metabolic process / response to xenobiotic stimulus / response to antibiotic 類似検索 - 分子機能
Dihydrofolate reductase, type II / R67 dihydrofolate reductase / SH3 type barrels. - #60 / Mechanosensitive ion channel MscS, beta-domain superfamily / Electron transport accessory-like domain superfamily / SH3 type barrels. / Roll / Mainly Beta 類似検索 - ドメイン・相同性
Chem-D49 / PHOSPHATE ION / Dihydrofolate reductase type 2 類似検索 - 構成要素
温度: 277 K / 手法: 蒸気拡散法, ハンギングドロップ法 / pH: 8 詳細: The protein concentration was adjusted from 13.3 mg/ml to 10 mg/mL by addition of a final concentration of 25% MPD. Reservoirs were prepared in Eppendorf tubes with 100mM Tris-Cl pH 8.0 55% ...詳細: The protein concentration was adjusted from 13.3 mg/ml to 10 mg/mL by addition of a final concentration of 25% MPD. Reservoirs were prepared in Eppendorf tubes with 100mM Tris-Cl pH 8.0 55% MPD in a Greiner 24-well hanging-drop crystallization plate. On a siliconized glass cover slip (Hampton Research), 2.0 uL of protein solution were combined with 2.0 uL of the reservoir solution. The plate was incubated at 277 K, and crystals were obtained after 3-4 days.
解像度: 1.75→47.86 Å / Cor.coef. Fo:Fc: 0.953 / Cor.coef. Fo:Fc free: 0.965 / SU B: 2.723 / SU ML: 0.081 / 交差検証法: THROUGHOUT / σ(F): 0 / ESU R: 0.121 / ESU R Free: 0.114 詳細: Authors state that inhibitor D49 is partially modelled at the active site. Binding of the ligand has been confirmed biochemically, and ligand disorder outside of the pore center has been ...詳細: Authors state that inhibitor D49 is partially modelled at the active site. Binding of the ligand has been confirmed biochemically, and ligand disorder outside of the pore center has been observed for other known ligands. More details can be found in the primary citation.