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- PDB-6nct: Structure of p110alpha/niSH2 - vector data collection -

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Basic information

Entry
Database: PDB / ID: 6nct
TitleStructure of p110alpha/niSH2 - vector data collection
Components
  • Phosphatidylinositol 3-kinase regulatory subunit alpha
  • Phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit alpha isoform
KeywordsTRANSFERASE / PI3K / kinase / Phosphatidylinositol 4 / 5-bisphosphate 3-kinase alpha isoform / p110 / p85
Function / homology
Function and homology information


perinuclear endoplasmic reticulum membrane / regulation of toll-like receptor 4 signaling pathway / response to muscle inactivity / phosphatidylinositol kinase activity / regulation of actin filament organization / positive regulation of focal adhesion disassembly / negative regulation of actin filament depolymerization / response to butyrate / 1-phosphatidylinositol-3-kinase regulator activity / phosphatidylinositol 3-kinase regulator activity ...perinuclear endoplasmic reticulum membrane / regulation of toll-like receptor 4 signaling pathway / response to muscle inactivity / phosphatidylinositol kinase activity / regulation of actin filament organization / positive regulation of focal adhesion disassembly / negative regulation of actin filament depolymerization / response to butyrate / 1-phosphatidylinositol-3-kinase regulator activity / phosphatidylinositol 3-kinase regulator activity / response to L-leucine / positive regulation of endoplasmic reticulum unfolded protein response / IRS-mediated signalling / phosphatidylinositol 3-kinase activator activity / T follicular helper cell differentiation / interleukin-18-mediated signaling pathway / PI3K events in ERBB4 signaling / phosphatidylinositol 3-kinase complex / phosphatidylinositol 3-kinase regulatory subunit binding / myeloid leukocyte migration / neurotrophin TRKA receptor binding / cellular response to hydrostatic pressure / autosome genomic imprinting / Activated NTRK2 signals through PI3K / regulation of cellular respiration / cis-Golgi network / negative regulation of fibroblast apoptotic process / transmembrane receptor protein tyrosine kinase adaptor activity / Activated NTRK3 signals through PI3K / ErbB-3 class receptor binding / phosphatidylinositol 3-kinase complex, class IB / negative regulation of stress fiber assembly / positive regulation of protein localization to membrane / 1-phosphatidylinositol-4-phosphate 3-kinase activity / Signaling by cytosolic FGFR1 fusion mutants / Co-stimulation by ICOS / RHOD GTPase cycle / vasculature development / cardiac muscle cell contraction / phosphatidylinositol 3-kinase complex, class IA / Nephrin family interactions / RHOF GTPase cycle / kinase activator activity / anoikis / Signaling by LTK in cancer / phosphatidylinositol-3-phosphate biosynthetic process / Signaling by LTK / positive regulation of leukocyte migration / relaxation of cardiac muscle / 1-phosphatidylinositol-4,5-bisphosphate 3-kinase activity / MET activates PI3K/AKT signaling / RND1 GTPase cycle / PI3K/AKT activation / RND2 GTPase cycle / phosphatidylinositol-4,5-bisphosphate 3-kinase / RND3 GTPase cycle / positive regulation of filopodium assembly / vascular endothelial growth factor signaling pathway / phosphatidylinositol 3-kinase / growth hormone receptor signaling pathway / insulin binding / Signaling by ALK / 1-phosphatidylinositol-3-kinase activity / RHOV GTPase cycle / RHOB GTPase cycle / Erythropoietin activates Phosphoinositide-3-kinase (PI3K) / natural killer cell mediated cytotoxicity / PI-3K cascade:FGFR3 / GP1b-IX-V activation signalling / negative regulation of macroautophagy / response to dexamethasone / PI-3K cascade:FGFR2 / PI-3K cascade:FGFR4 / phosphatidylinositol-mediated signaling / PI-3K cascade:FGFR1 / RHOC GTPase cycle / RHOJ GTPase cycle / negative regulation of osteoclast differentiation / phosphatidylinositol phosphate biosynthetic process / Synthesis of PIPs at the plasma membrane / intracellular glucose homeostasis / RHOU GTPase cycle / CDC42 GTPase cycle / RET signaling / negative regulation of anoikis / insulin receptor substrate binding / Interleukin-3, Interleukin-5 and GM-CSF signaling / PI3K events in ERBB2 signaling / intercalated disc / PI3K Cascade / T cell differentiation / negative regulation of cell-matrix adhesion / RHOG GTPase cycle / extrinsic apoptotic signaling pathway via death domain receptors / CD28 dependent PI3K/Akt signaling / regulation of multicellular organism growth / Role of LAT2/NTAL/LAB on calcium mobilization / RHOA GTPase cycle / RAC2 GTPase cycle / RAC3 GTPase cycle
Similarity search - Function
Helix Hairpins - #1490 / PI3Kalpha, catalytic domain / Phosphatidylinositol 3-kinase regulatory subunit alpha, SH3 domain / PIK3R1, inter-SH2 domain / PI3K p85 subunit, C-terminal SH2 domain / PI3K regulatory subunit p85-related , inter-SH2 domain / PI3K p85 subunit, N-terminal SH2 domain / Phosphatidylinositol 3-kinase regulatory subunit P85 inter-SH2 domain / PI3-kinase family, p85-binding domain / PI3-kinase family, p85-binding domain ...Helix Hairpins - #1490 / PI3Kalpha, catalytic domain / Phosphatidylinositol 3-kinase regulatory subunit alpha, SH3 domain / PIK3R1, inter-SH2 domain / PI3K p85 subunit, C-terminal SH2 domain / PI3K regulatory subunit p85-related , inter-SH2 domain / PI3K p85 subunit, N-terminal SH2 domain / Phosphatidylinositol 3-kinase regulatory subunit P85 inter-SH2 domain / PI3-kinase family, p85-binding domain / PI3-kinase family, p85-binding domain / Phosphatidylinositol 3-kinase, accessory domain (PIK) / Rho GTPase-activating protein domain / RhoGAP domain / Rho GTPase-activating proteins domain profile. / GTPase-activator protein for Rho-like GTPases / Phosphatidylinositol 3-kinase, adaptor-binding domain / Phosphatidylinositol 3-kinase adaptor-binding (PI3K ABD) domain profile. / PI3-kinase family, Ras-binding domain / Phosphatidylinositol 3-kinase Ras-binding (PI3K RBD) domain / PI3-kinase family, ras-binding domain / Phosphatidylinositol 3-kinase Ras-binding (PI3K RBD) domain profile. / Rho GTPase activation protein / C2 phosphatidylinositol 3-kinase-type domain / Phosphoinositide 3-kinase C2 / C2 phosphatidylinositol 3-kinase (PI3K)-type domain profile. / Phosphoinositide 3-kinase, region postulated to contain C2 domain / Phosphoinositide 3-kinase family, accessory domain (PIK domain) / Phosphoinositide 3-kinase family, accessory domain (PIK domain) / Phosphoinositide 3-kinase, accessory (PIK) domain superfamily / C2 domain / Phosphoinositide 3-kinase, accessory (PIK) domain / Phosphatidylinositol kinase / PIK helical domain profile. / Phosphatidylinositol 3- and 4-kinases signature 1. / Phosphatidylinositol 3/4-kinase, conserved site / Phosphatidylinositol 3- and 4-kinases signature 2. / Phosphatidylinositol 3-/4-kinase, catalytic domain superfamily / Phosphoinositide 3-kinase, catalytic domain / Phosphatidylinositol 3- and 4-kinase / Phosphatidylinositol 3- and 4-kinases catalytic domain profile. / Phosphatidylinositol 3-/4-kinase, catalytic domain / C2 domain superfamily / SH2 domain / Src homology 2 (SH2) domain profile. / Src homology 2 domains / SH2 domain / Src homology 3 domains / SH2 domain superfamily / SH3-like domain superfamily / Src homology 3 (SH3) domain profile. / SH3 domain / Serine Threonine Protein Phosphatase 5, Tetratricopeptide repeat / Helix Hairpins / Alpha Horseshoe / Armadillo-type fold / Ubiquitin-like domain superfamily / Protein kinase-like domain superfamily / Immunoglobulin-like / Sandwich / Orthogonal Bundle / Mainly Beta / Mainly Alpha
Similarity search - Domain/homology
TRIS-HYDROXYMETHYL-METHYL-AMMONIUM / Phosphatidylinositol 3-kinase regulatory subunit alpha / Phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit alpha isoform
Similarity search - Component
Biological speciesHomo sapiens (human)
MethodX-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / molecular replacement / Resolution: 3.35 Å
AuthorsMiller, M.S. / Maheshwari, S. / Amzel, L.M. / Gabelli, S.B.
Funding support United States, 1items
OrganizationGrant numberCountry
National Institutes of Health/National Cancer Institute (NIH/NCI)CA062924 United States
CitationJournal: Molecules / Year: 2019
Title: Getting the Most Out of Your Crystals: Data Collection at the New High-Flux, Microfocus MX Beamlines at NSLS-II.
Authors: Miller, M.S. / Maheshwari, S. / Shi, W. / Gao, Y. / Chu, N. / Soares, A.S. / Cole, P.A. / Amzel, L.M. / Fuchs, M.R. / Jakoncic, J. / Gabelli, S.B.
History
DepositionDec 12, 2018Deposition site: RCSB / Processing site: RCSB
Revision 1.0Feb 6, 2019Provider: repository / Type: Initial release
Revision 1.1Nov 13, 2019Group: Database references / Category: citation / citation_author
Item: _citation.journal_volume / _citation.page_first ..._citation.journal_volume / _citation.page_first / _citation.pdbx_database_id_PubMed / _citation.title / _citation_author.identifier_ORCID
Revision 1.2Dec 4, 2019Group: Author supporting evidence / Category: pdbx_audit_support / Item: _pdbx_audit_support.funding_organization
Revision 1.3Oct 11, 2023Group: Data collection / Database references / Refinement description
Category: chem_comp_atom / chem_comp_bond ...chem_comp_atom / chem_comp_bond / database_2 / pdbx_initial_refinement_model
Item: _database_2.pdbx_DOI / _database_2.pdbx_database_accession

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Assembly

Deposited unit
A: Phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit alpha isoform
B: Phosphatidylinositol 3-kinase regulatory subunit alpha
hetero molecules


Theoretical massNumber of molelcules
Total (without water)162,0227
Polymers161,4902
Non-polymers5325
Water00
1


  • Idetical with deposited unit
  • defined by author&software
  • Evidence: gel filtration
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area6740 Å2
ΔGint-39 kcal/mol
Surface area63190 Å2
MethodPISA
Unit cell
Length a, b, c (Å)115.355, 117.724, 151.331
Angle α, β, γ (deg.)90.000, 90.000, 90.000
Int Tables number19
Space group name H-MP212121

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Components

#1: Protein Phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit alpha isoform / PtdIns-3-kinase subunit alpha / Phosphatidylinositol 4 / 5-bisphosphate 3-kinase 110 kDa catalytic ...PtdIns-3-kinase subunit alpha / Phosphatidylinositol 4 / 5-bisphosphate 3-kinase 110 kDa catalytic subunit alpha / p110alpha / Phosphoinositide-3-kinase catalytic alpha polypeptide / Serine/threonine protein kinase PIK3CA


Mass: 127822.578 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human) / Gene: PIK3CA / Production host: Spodoptera frugiperda (fall armyworm)
References: UniProt: P42336, phosphatidylinositol-4,5-bisphosphate 3-kinase, non-specific serine/threonine protein kinase
#2: Protein Phosphatidylinositol 3-kinase regulatory subunit alpha / PtdIns-3-kinase regulatory subunit alpha / Phosphatidylinositol 3-kinase 85 kDa regulatory subunit ...PtdIns-3-kinase regulatory subunit alpha / Phosphatidylinositol 3-kinase 85 kDa regulatory subunit alpha / PtdIns-3-kinase regulatory subunit p85-alpha


Mass: 33666.961 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human) / Gene: PIK3R1, GRB1 / Production host: Spodoptera frugiperda (fall armyworm) / References: UniProt: P27986
#3: Chemical ChemComp-144 / TRIS-HYDROXYMETHYL-METHYL-AMMONIUM


Mass: 122.143 Da / Num. of mol.: 2 / Source method: obtained synthetically / Formula: C4H12NO3
#4: Chemical ChemComp-SO4 / SULFATE ION


Mass: 96.063 Da / Num. of mol.: 3 / Source method: obtained synthetically / Formula: SO4

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Experimental details

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Experiment

ExperimentMethod: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

CrystalDensity Matthews: 3.18 Å3/Da / Density % sol: 61.34 %
Crystal growTemperature: 298 K / Method: vapor diffusion, hanging drop / pH: 7 / Details: 0.1 M Hepes, 1.4-1.6 M sodium formate

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Data collection

DiffractionMean temperature: 100 K / Serial crystal experiment: N
Diffraction sourceSource: SYNCHROTRON / Site: NSLS-II / Beamline: 17-ID-1 / Wavelength: 0.9199 Å
DetectorType: DECTRIS EIGER X 9M / Detector: PIXEL / Date: Jun 12, 2018
RadiationProtocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelengthWavelength: 0.9199 Å / Relative weight: 1
ReflectionResolution: 3.35→49.54 Å / Num. obs: 29227 / % possible obs: 96.4 % / Redundancy: 5.442 % / Biso Wilson estimate: 92.96 Å2 / CC1/2: 0.998 / Rmerge(I) obs: 0.102 / Rrim(I) all: 0.112 / Χ2: 1.049 / Net I/σ(I): 11.56 / Num. measured all: 159053 / Scaling rejects: 7
Reflection shell

Diffraction-ID: 1

Resolution (Å)Redundancy (%)Rmerge(I) obsMean I/σ(I) obsNum. measured obsNum. possibleNum. unique obsCC1/2Rrim(I) all% possible all
3.35-3.445.4010.9781.5611505219721300.7041.07597
3.44-3.535.2630.7192.0911104215021100.7660.79398.1
3.53-3.635.1270.5252.8610547209720570.8830.5898.1
3.63-3.745.7320.4043.9911481204420030.9280.44298
3.74-3.875.7220.3085.1711020197119260.9540.33697.7
3.87-45.6890.2466.6210672192918760.970.26997.3
4-4.155.7010.2017.9910171183117840.980.22197.4
4.15-4.325.6980.1669.899847179317280.9850.18296.4
4.32-4.525.6090.12612.489278170316540.990.13897.1
4.52-4.745.560.10714.988835164615890.9930.11896.5
4.74-4.995.5280.09616.228381156715160.9930.10696.7
4.99-5.35.3760.08916.87709148514340.9940.09896.6
5.3-5.665.2910.09216.387069139413360.9940.10295.8
5.66-6.114.8740.08916.096107131412530.9930.09995.4
6.11-6.75.0130.07818.265725119711420.9950.08695.4
6.7-7.494.9720.06521.425231111010520.9960.07294.8
7.49-8.655.5770.0528.5651709889270.9970.05593.8
8.65-10.595.3830.04132.842208407840.9990.04593.3
10.59-14.985.5110.0434.8233456726070.9980.04490.3
14.98-49.545.1290.03933.2216363953190.9970.04380.8

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Phasing

PhasingMethod: molecular replacement
Phasing MRR rigid body: 0.464
Highest resolutionLowest resolution
Rotation49.54 Å3.5 Å

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Processing

Software
NameVersionClassification
REFMAC5.8.0238refinement
XSCALEdata scaling
MOLREPphasing
PDB_EXTRACT3.24data extraction
XDSdata reduction
RefinementMethod to determine structure: MOLECULAR REPLACEMENT
Starting model: 4OVU

4ovu


Resolution: 3.35→49.54 Å / Cor.coef. Fo:Fc: 0.949 / Cor.coef. Fo:Fc free: 0.916 / WRfactor Rfree: 0.2733 / WRfactor Rwork: 0.2042 / FOM work R set: 0.7287 / SU B: 78.481 / SU ML: 0.55 / SU Rfree: 0.6131 / Cross valid method: THROUGHOUT / σ(F): 0 / ESU R Free: 0.575 / Stereochemistry target values: MAXIMUM LIKELIHOOD
Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS U VALUES : WITH TLS ADDED
RfactorNum. reflection% reflectionSelection details
Rfree0.2709 1462 5 %RANDOM
Rwork0.1992 ---
obs0.2028 27766 96.39 %-
Solvent computationIon probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å / Solvent model: MASK
Displacement parametersBiso max: 337.39 Å2 / Biso mean: 123.652 Å2 / Biso min: 30 Å2
Baniso -1Baniso -2Baniso -3
1--3.17 Å20 Å20 Å2
2--5.11 Å20 Å2
3----1.94 Å2
Refinement stepCycle: final / Resolution: 3.35→49.54 Å
ProteinNucleic acidLigandSolventTotal
Num. atoms10462 0 31 0 10493
Biso mean--189.18 --
Num. residues----1264
Refine LS restraints
Refine-IDTypeDev idealDev ideal targetNumber
X-RAY DIFFRACTIONr_bond_refined_d0.0130.01310731
X-RAY DIFFRACTIONr_bond_other_d0.0020.0179977
X-RAY DIFFRACTIONr_angle_refined_deg1.9521.64414469
X-RAY DIFFRACTIONr_angle_other_deg1.4351.57723207
X-RAY DIFFRACTIONr_dihedral_angle_1_deg6.67551248
X-RAY DIFFRACTIONr_dihedral_angle_2_deg32.30122.553615
X-RAY DIFFRACTIONr_dihedral_angle_3_deg19.587152009
X-RAY DIFFRACTIONr_dihedral_angle_4_deg20.3291573
X-RAY DIFFRACTIONr_chiral_restr0.0830.21357
X-RAY DIFFRACTIONr_gen_planes_refined0.0080.0211685
X-RAY DIFFRACTIONr_gen_planes_other0.0020.022242
LS refinement shellResolution: 3.349→3.436 Å / Rfactor Rfree error: 0 / Total num. of bins used: 20
RfactorNum. reflection% reflection
Rfree0.343 106 -
Rwork0.314 2013 -
all-2119 -
obs--96.94 %
Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

IDL11 (°2)L12 (°2)L13 (°2)L22 (°2)L23 (°2)L33 (°2)S11 (Å °)S12 (Å °)S13 (Å °)S21 (Å °)S22 (Å °)S23 (Å °)S31 (Å °)S32 (Å °)S33 (Å °)T11 (Å2)T12 (Å2)T13 (Å2)T22 (Å2)T23 (Å2)T33 (Å2)Origin x (Å)Origin y (Å)Origin z (Å)
16.8953-3.3928-2.013611.53867.19447.6641-0.31960.3861-0.14760.59550.34940.5340.4405-0.0452-0.02990.2151-0.2089-0.14330.43240.36920.36567.800824.199-5.8587
21.08730.06130.63770.58650.03071.68150.13120.2232-0.01370.1203-0.1404-0.1701-0.05810.13260.00930.08880.057-0.04380.18060.02920.051148.0482-4.222817.469
310.35485.1341-2.45154.294-1.14472.22560.3335-0.71760.33430.1668-0.22480.1852-0.446-0.2602-0.10880.48130.3179-0.13290.3273-0.10780.070127.273218.012323.9572
Refinement TLS group
IDRefine-IDRefine TLS-IDAuth asym-IDAuth seq-ID
1X-RAY DIFFRACTION1B329 - 434
2X-RAY DIFFRACTION2A-26 - 1057
3X-RAY DIFFRACTION3B439 - 597

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