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- PDB-6n1c: Crystal structure of Inorganic pyrophosphatase from Legionella pn... -

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Basic information

Entry
Database: PDB / ID: 6n1c
TitleCrystal structure of Inorganic pyrophosphatase from Legionella pneumophila Philadelphia 1
ComponentsInorganic pyrophosphatase
KeywordsHYDROLASE / SSGCID / Structural Genomics / Seattle Structural Genomics Center for Infectious Disease
Function / homology
Function and homology information


inorganic diphosphatase / inorganic diphosphate phosphatase activity / phosphate-containing compound metabolic process / magnesium ion binding / cytosol
Similarity search - Function
Inorganic Pyrophosphatase / Inorganic pyrophosphatase / Inorganic pyrophosphatase signature. / Inorganic pyrophosphatase / Inorganic pyrophosphatase superfamily / Inorganic pyrophosphatase / Alpha-Beta Complex / Alpha Beta
Similarity search - Domain/homology
ALANINE / Inorganic pyrophosphatase
Similarity search - Component
Biological speciesLegionella pneumophila subsp. pneumophila (bacteria)
MethodX-RAY DIFFRACTION / MOLECULAR REPLACEMENT / Resolution: 2 Å
AuthorsSeattle Structural Genomics Center for Infectious Disease (SSGCID)
CitationJournal: Acta Crystallogr.,Sect.F / Year: 2023
Title: Characterization of a family I inorganic pyrophosphatase from Legionella pneumophila Philadelphia 1.
Authors: Moorefield, J. / Konuk, Y. / Norman, J.O. / Abendroth, J. / Edwards, T.E. / Lorimer, D.D. / Mayclin, S.J. / Staker, B.L. / Craig, J.K. / Barett, K.F. / Barrett, L.K. / Van Voorhis, W.C. / ...Authors: Moorefield, J. / Konuk, Y. / Norman, J.O. / Abendroth, J. / Edwards, T.E. / Lorimer, D.D. / Mayclin, S.J. / Staker, B.L. / Craig, J.K. / Barett, K.F. / Barrett, L.K. / Van Voorhis, W.C. / Myler, P.J. / McLaughlin, K.J.
History
DepositionNov 8, 2018Deposition site: RCSB / Processing site: RCSB
Revision 1.0Dec 19, 2018Provider: repository / Type: Initial release
Revision 1.1Oct 11, 2023Group: Data collection / Database references ...Data collection / Database references / Derived calculations / Refinement description
Category: chem_comp_atom / chem_comp_bond ...chem_comp_atom / chem_comp_bond / database_2 / pdbx_initial_refinement_model / pdbx_struct_conn_angle / struct_conn
Item: _database_2.pdbx_DOI / _database_2.pdbx_database_accession ..._database_2.pdbx_DOI / _database_2.pdbx_database_accession / _pdbx_struct_conn_angle.ptnr1_auth_seq_id / _pdbx_struct_conn_angle.ptnr3_auth_seq_id / _pdbx_struct_conn_angle.value / _struct_conn.pdbx_dist_value / _struct_conn.ptnr1_auth_asym_id / _struct_conn.ptnr1_auth_comp_id / _struct_conn.ptnr1_auth_seq_id / _struct_conn.ptnr1_label_asym_id / _struct_conn.ptnr1_label_atom_id / _struct_conn.ptnr1_label_comp_id / _struct_conn.ptnr1_label_seq_id / _struct_conn.ptnr2_auth_asym_id / _struct_conn.ptnr2_auth_comp_id / _struct_conn.ptnr2_auth_seq_id / _struct_conn.ptnr2_label_asym_id / _struct_conn.ptnr2_label_atom_id / _struct_conn.ptnr2_label_comp_id
Revision 1.2Jul 17, 2024Group: Database references / Category: citation / citation_author
Item: _citation.country / _citation.journal_abbrev ..._citation.country / _citation.journal_abbrev / _citation.journal_id_ASTM / _citation.journal_id_CSD / _citation.journal_id_ISSN / _citation.journal_volume / _citation.page_first / _citation.page_last / _citation.pdbx_database_id_DOI / _citation.pdbx_database_id_PubMed / _citation.title / _citation.year

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Assembly

Deposited unit
A: Inorganic pyrophosphatase
B: Inorganic pyrophosphatase
C: Inorganic pyrophosphatase
D: Inorganic pyrophosphatase
E: Inorganic pyrophosphatase
F: Inorganic pyrophosphatase
hetero molecules


Theoretical massNumber of molelcules
Total (without water)127,94920
Polymers126,7336
Non-polymers1,21614
Water8,647480
1


  • Idetical with deposited unit
  • defined by author&software
  • Evidence: gel filtration
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area15800 Å2
ΔGint-249 kcal/mol
Surface area37610 Å2
MethodPISA
Unit cell
Length a, b, c (Å)64.080, 119.940, 74.900
Angle α, β, γ (deg.)90.000, 109.590, 90.000
Int Tables number4
Space group name H-MP1211
Noncrystallographic symmetry (NCS)NCS domain:
IDEns-IDDetails
11(chain A and ((resid 11 and (name N or name...
21(chain B and ((resid 11 and (name N or name...
31(chain C and (resid 11 or resid 13 through 40...
41(chain D and ((resid 11 and (name N or name...
51(chain E and ((resid 11 and (name N or name...
61(chain F and ((resid 11 and (name N or name...

NCS domain segments:
Dom-IDComponent-IDEns-IDSelection detailsAuth asym-IDAuth seq-ID
111(chain A and ((resid 11 and (name N or name...A11
121(chain A and ((resid 11 and (name N or name...A4 - 178
131(chain A and ((resid 11 and (name N or name...A4 - 178
141(chain A and ((resid 11 and (name N or name...A4 - 178
151(chain A and ((resid 11 and (name N or name...A4 - 178
211(chain B and ((resid 11 and (name N or name...B11
221(chain B and ((resid 11 and (name N or name...B5 - 202
231(chain B and ((resid 11 and (name N or name...B5 - 202
241(chain B and ((resid 11 and (name N or name...B5 - 202
251(chain B and ((resid 11 and (name N or name...B5 - 202
311(chain C and (resid 11 or resid 13 through 40...C11
321(chain C and (resid 11 or resid 13 through 40...C13 - 40
331(chain C and (resid 11 or resid 13 through 40...C42 - 45
341(chain C and (resid 11 or resid 13 through 40...C11 - 177
351(chain C and (resid 11 or resid 13 through 40...C11 - 177
361(chain C and (resid 11 or resid 13 through 40...C119
371(chain C and (resid 11 or resid 13 through 40...C11 - 177
381(chain C and (resid 11 or resid 13 through 40...C11 - 177
391(chain C and (resid 11 or resid 13 through 40...C11 - 177
411(chain D and ((resid 11 and (name N or name...D11
421(chain D and ((resid 11 and (name N or name...D6 - 176
431(chain D and ((resid 11 and (name N or name...D6 - 176
441(chain D and ((resid 11 and (name N or name...D6 - 176
451(chain D and ((resid 11 and (name N or name...D6 - 176
511(chain E and ((resid 11 and (name N or name...E11
521(chain E and ((resid 11 and (name N or name...E4 - 177
531(chain E and ((resid 11 and (name N or name...E4 - 177
541(chain E and ((resid 11 and (name N or name...E4 - 177
551(chain E and ((resid 11 and (name N or name...E4 - 177
611(chain F and ((resid 11 and (name N or name...F11
621(chain F and ((resid 11 and (name N or name...F6 - 203
631(chain F and ((resid 11 and (name N or name...F6 - 203
641(chain F and ((resid 11 and (name N or name...F6 - 203
651(chain F and ((resid 11 and (name N or name...F6 - 203

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Components

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Protein , 1 types, 6 molecules ABCDEF

#1: Protein
Inorganic pyrophosphatase / Pyrophosphate phospho-hydrolase / PPase


Mass: 21122.242 Da / Num. of mol.: 6
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Legionella pneumophila subsp. pneumophila (strain Philadelphia 1 / ATCC 33152 / DSM 7513) (bacteria)
Strain: Philadelphia 1 / ATCC 33152 / DSM 7513 / Gene: ppa, lpg2764 / Plasmid: LepnA.00023.a.B1 / Production host: Escherichia coli BL21(DE3) (bacteria) / Strain (production host): BL21(DE3) / References: UniProt: Q5ZRW2, inorganic diphosphatase

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Non-polymers , 5 types, 494 molecules

#2: Chemical
ChemComp-NA / SODIUM ION


Mass: 22.990 Da / Num. of mol.: 4 / Source method: obtained synthetically / Formula: Na
#3: Chemical
ChemComp-MPD / (4S)-2-METHYL-2,4-PENTANEDIOL


Mass: 118.174 Da / Num. of mol.: 7 / Source method: obtained synthetically / Formula: C6H14O2 / Comment: precipitant*YM
#4: Chemical ChemComp-ALA / ALANINE


Type: L-peptide linking / Mass: 89.093 Da / Num. of mol.: 2 / Source method: obtained synthetically / Formula: C3H7NO2
#5: Chemical ChemComp-MRD / (4R)-2-METHYLPENTANE-2,4-DIOL


Mass: 118.174 Da / Num. of mol.: 1 / Source method: obtained synthetically / Formula: C6H14O2 / Comment: precipitant*YM
#6: Water ChemComp-HOH / water


Mass: 18.015 Da / Num. of mol.: 480 / Source method: isolated from a natural source / Formula: H2O

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Experimental details

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Experiment

ExperimentMethod: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

CrystalDensity Matthews: 2.14 Å3/Da / Density % sol: 42.6 %
Crystal growTemperature: 285 K / Method: vapor diffusion, sitting drop / pH: 8.5
Details: Molecular dimensions Morpheus screen, H12: 12.5% PEG 1000, 12.5% PEG 3350, 12.5% MPD: 20mM of each sodium L-glutamate, DL-alanine, glycine, DL-lysine HCl, DL-serine: 100mM Bicine/Trizma base ...Details: Molecular dimensions Morpheus screen, H12: 12.5% PEG 1000, 12.5% PEG 3350, 12.5% MPD: 20mM of each sodium L-glutamate, DL-alanine, glycine, DL-lysine HCl, DL-serine: 100mM Bicine/Trizma base pH 8.5: LepnA.00023.a.B1.PS38421 at 21.3mg/ml: cryo: direct: tray 299905h12: puck hgu1-7

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Data collection

DiffractionMean temperature: 100 K / Serial crystal experiment: N
Diffraction sourceSource: ROTATING ANODE / Type: RIGAKU FR-E+ SUPERBRIGHT / Wavelength: 1.5418 Å
DetectorType: RIGAKU SATURN 944+ / Detector: CCD / Date: May 11, 2018
RadiationMonochromator: RIGAKU VARIMAX / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelengthWavelength: 1.5418 Å / Relative weight: 1
ReflectionResolution: 2→40.967 Å / Num. obs: 71673 / % possible obs: 99.5 % / Redundancy: 6.018 % / Biso Wilson estimate: 43.609 Å2 / CC1/2: 0.999 / Rmerge(I) obs: 0.048 / Rrim(I) all: 0.053 / Χ2: 0.989 / Net I/σ(I): 19.31
Reflection shell

Diffraction-ID: 1

Resolution (Å)Redundancy (%)Rmerge(I) obsMean I/σ(I) obsNum. unique obsCC1/2Rrim(I) all% possible all
2-2.053.9940.6232.2552770.7850.71399.9
2.05-2.114.4090.4963.0851670.8720.56199.9
2.11-2.174.8140.3914.1349950.9250.43899.8
2.17-2.245.0860.2945.6448800.9570.32799.5
2.24-2.315.3940.2486.8247330.9720.27599.4
2.31-2.395.890.2088.4645570.9810.22899.4
2.39-2.486.6450.17210.7144160.9890.18799.4
2.48-2.586.8560.15711.4942380.9910.1799.5
2.58-2.76.8760.13213.3540430.9940.14399.5
2.7-2.836.9060.09617.3839000.9960.10399.6
2.83-2.986.920.06922.8236950.9980.07599.3
2.98-3.166.9330.05926.2534860.9980.06399.6
3.16-3.386.9240.04732.1133420.9990.05199.7
3.38-3.656.8540.03939.2330400.9990.04299.5
3.65-46.7160.03545.5128440.9990.03899.4
4-4.476.6960.0350.6625410.9990.03299.5
4.47-5.166.6680.02854.1622730.9990.0399.5
5.16-6.326.6990.02852.6719180.9990.03199.5
6.32-8.946.6930.02656.9315030.9990.02999.7
8.94-40.9676.2910.02460.18250.9990.02697.6

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Processing

Software
NameVersionClassification
XDSdata reduction
XSCALEdata scaling
PHENIX(dev_3304)refinement
PDB_EXTRACT3.24data extraction
MoRDaphasing
ARP/wARPmodel building
BUCCANEERmodel building
Cootmodel building
RefinementMethod to determine structure: MOLECULAR REPLACEMENT
Starting model: pdb entry 4xel as per Morda

4xel


Resolution: 2→40.967 Å / SU ML: 0.21 / Cross valid method: THROUGHOUT / σ(F): 1.34 / Phase error: 27.18
RfactorNum. reflection% reflectionSelection details
Rfree0.2321 1980 2.76 %0
Rwork0.1934 ---
obs0.1945 71619 99.66 %-
Solvent computationShrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å
Displacement parametersBiso max: 121.38 Å2 / Biso mean: 49.0626 Å2 / Biso min: 20.22 Å2
Refinement stepCycle: final / Resolution: 2→40.967 Å
ProteinNucleic acidLigandSolventTotal
Num. atoms7826 0 80 480 8386
Biso mean--61.22 46.77 -
Num. residues----1029
Refine LS restraints
Refine-IDTypeDev idealNumber
X-RAY DIFFRACTIONf_bond_d0.0078186
X-RAY DIFFRACTIONf_angle_d0.8811213
X-RAY DIFFRACTIONf_dihedral_angle_d12.3214888
X-RAY DIFFRACTIONf_chiral_restr0.0631297
X-RAY DIFFRACTIONf_plane_restr0.0071435
Refine LS restraints NCS
Ens-IDDom-IDAuth asym-IDNumberRefine-IDRmsType
11A4229X-RAY DIFFRACTION8.287TORSIONAL
12B4229X-RAY DIFFRACTION8.287TORSIONAL
13C4229X-RAY DIFFRACTION8.287TORSIONAL
14D4229X-RAY DIFFRACTION8.287TORSIONAL
15E4229X-RAY DIFFRACTION8.287TORSIONAL
16F4229X-RAY DIFFRACTION8.287TORSIONAL
LS refinement shell

Refine-ID: X-RAY DIFFRACTION / Rfactor Rfree error: 0 / Total num. of bins used: 14

Resolution (Å)Rfactor RfreeNum. reflection RfreeRfactor RworkNum. reflection RworkNum. reflection all% reflection obs (%)
2-2.050.2991230.260249565079100
2.05-2.10550.27561420.251649645106100
2.1055-2.16740.32631450.238649685113100
2.1674-2.23740.28341230.222949825105100
2.2374-2.31730.26531220.219750005122100
2.3173-2.41010.26491350.21749485083100
2.4101-2.51980.3191480.22074956510499
2.5198-2.65260.31121410.24054955509699
2.6526-2.81880.23331430.228449545097100
2.8188-3.03630.24741610.21574957511899
3.0363-3.34180.2681390.202349925131100
3.3418-3.8250.20851480.181749735121100
3.825-4.81790.17731670.149149695136100
4.8179-40.97540.21411430.171550655208100
Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

IDL11 (°2)L12 (°2)L13 (°2)L22 (°2)L23 (°2)L33 (°2)S11 (Å °)S12 (Å °)S13 (Å °)S21 (Å °)S22 (Å °)S23 (Å °)S31 (Å °)S32 (Å °)S33 (Å °)T11 (Å2)T12 (Å2)T13 (Å2)T22 (Å2)T23 (Å2)T33 (Å2)Origin x (Å)Origin y (Å)Origin z (Å)
15.5295-2.7881-0.67326.1218-0.36712.6360.54-0.11780.79890.4025-0.3618-0.6911-1.1350.5824-0.12030.7836-0.16490.1890.7413-0.10680.468316.162434.380750.7932
25.4178-2.2693-2.5297.66093.38323.59030.10190.0438-0.22810.35740.1525-0.64240.35290.133-0.27160.1969-0.008-0.02670.217-0.00170.366925.21325.421735.3976
31.75311.0354-0.05922.93160.65442.88230.08450.10630.09310.2031-0.15210.24870.1901-0.21030.07540.1653-0.0110.06050.23180.0080.226513.021128.908734.2326
43.0919-0.23960.042.1703-0.00466.24140.53-0.649-0.1011.0317-0.25570.7690.6988-0.7732-0.27160.5114-0.12970.13260.30540.02810.33748.76224.191146.8031
52222-6.078920.5902-1.93542.66441.1895-1.30591.1677-2.282-3.99760.71610.86910.0795-0.21120.9563-0.23460.429420.49440.763224.2258
61.2189-0.1150.66915.88593.37242.3737-0.08240.19470.1826-1.0665-0.31790.1401-0.49790.4662-0.00421.3303-0.17260.0830.8784-0.30980.308928.475326.9412-9.7944
72.1857-0.957-1.38481.8221.72131.7604-0.10350.18450.0301-0.86080.04360.276-0.3585-0.08730.08060.5311-0.0461-0.07240.4091-0.08050.245218.426134.10647.6624
82.4524-2.6482-0.9524.2976-1.1053.5715-0.28311.1547-0.1574-0.81010.29540.7441-0.0715-0.5361-0.06690.927-0.1603-0.29280.7854-0.03770.376815.372529.018-6.2391
91.23850.7266-0.82412.20790.35892.2902-0.32130.4522-0.5517-0.64720.24140.09910.0746-0.26720.00050.4802-0.0939-0.02340.4886-0.15140.278420.546221.30753.3573
102.81752.3741-1.71235.2866-3.36712.171-0.12210.60450.2804-0.29650.1748-0.1226-1.27640.4726-0.07661.013-0.30550.02390.48060.05420.19641.819853.44882.2124
116.07720.11370.92416.5392-1.13975.6214-0.19120.7688-0.0097-1.22280.18910.14480.31050.107-0.07240.5749-0.20950.11550.4403-0.03540.385836.737738.83254.2955
121.94151.4497-0.12021.0411-0.05780.0663-0.36560.5217-0.5279-0.66310.3769-0.4626-0.05350.1193-0.03630.453-0.11760.13690.4075-0.10780.333841.198336.52346.6858
133.94192.1259-2.28454.71932.73089.3292-0.08561.001-0.4741-1.41710.0775-0.80210.37990.48070.06631.0331-0.27980.22090.9011-0.09260.396745.474546.6625-6.2009
141.56870.2455-0.53050.0633-0.23252.4725-0.32560.83110.07-1.0460.4218-0.2014-0.72630.6101-0.19240.7236-0.33050.27690.5743-0.10610.224745.962448.72946.7783
152.86841.5952-0.03414.1725-0.03440.92130.02350.19490.5043-0.44940.371-0.3816-0.81340.7332-0.34920.7504-0.30440.12020.5987-0.1170.37451.525856.747415.3278
161.7185-1.9468-1.24093.65740.23971.8918-0.03740.5758-0.4768-0.99270.3722-0.9234-0.21830.6255-0.18290.3522-0.02850.16310.6332-0.17620.612252.624336.176910.3179
170.87220.1504-0.04861.1096-0.12410.13260.0790.72710.3119-0.9210.1958-0.0075-0.71040.21330.06130.884-0.75480.75531.175-0.09220.269352.626451.0793-2.9769
18228.58972-7.221120.6933-2.8624.22141.8584-0.4516-1.6857-2.32910.6562-0.25991.03060.2328-0.19280.6578-0.15440.731938.151244.877822.2334
190.4501-0.35120.11771.34130.15274.9151-0.3108-0.08020.1208-0.3255-0.1404-0.0323-0.4701-0.22420.17081.0185-0.037-0.50470.65470.13360.92024.896252.81844.1854
206.9573-0.07251.10030.33890.35260.8605-0.22460.62970.2075-1.12230.11810.306-0.2399-0.02110.04360.67990.0048-0.16080.30690.00110.329623.980252.334610.263
213.50190.50330.87070.41530.27792.42760.09330.30950.1414-0.615-0.09690.0677-0.5031-0.02630.02610.53560.0185-0.10440.2401-0.00240.257626.906155.245615.7231
222.2336-1.65060.14492.1196-0.20313.4517-0.10090.23050.6315-1.1353-0.24970.1712-0.66320.07850.28420.65430.0022-0.23040.3279-0.01390.416516.506454.380810.3077
231.45351.59560.18923.93582.15681.75450.16350.12210.2706-1.0182-0.25091.1262-0.6584-0.33680.3190.66130.1306-0.32690.3199-0.04660.44212.993459.69715.6542
241.3546-1.3947-0.76671.63121.35062.16080.01550.24810.0142-0.3868-0.71131.4733-0.1064-0.5790.49520.35510.0696-0.19670.5464-0.15291.00573.904944.960418.1092
251.63930.64870.52985.24490.97411.7024-0.0851-0.31720.0897-0.5738-0.22260.6677-0.3922-0.16030.21110.45710.0659-0.07540.2843-0.03230.32418.860960.17124.9414
261.1320.04240.8902-0.0112-0.06891.4635-0.15620.2170.6583-1.1306-0.08961.2163-0.5073-0.15280.07561.01460.1846-0.55870.42210.06120.87278.896764.146310.8239
278.16391.70080.87272.4718-0.3043.120.0129-0.4997-0.63220.6072-0.0089-0.822-0.04840.2084-0.05420.319-0.0361-0.10580.2321-0.00430.41635.256343.115141.8772
282.57160.10610.26323.2863-0.21941.3977-0.0169-0.2305-0.0180.24720.0339-0.5213-0.20680.1261-0.00230.2252-0.047-0.05760.236-0.04480.316339.45153.721339.8489
295.72982.77461.73992.01771.99012.4664-0.0641-0.14561.2654-0.9175-0.4761-0.2305-0.35170.00230.3890.83890.1828-0.09670.4190.17831.326439.35669.705637.5401
300.3701-0.0029-0.79022.57090.2231.710.16760.014-0.44520.27650.1673-0.63720.07740.4648-0.26880.27180.0329-0.12460.3156-0.05310.806745.223328.065232.0661
311.7510.3259-0.58811.49750.36741.0339-0.00650.1142-0.61520.08510.0531-0.85540.10520.3290.00550.2960.0459-0.07530.3115-0.03820.869546.681618.119825.9143
Refinement TLS group
IDRefine-IDRefine TLS-IDSelection detailsAuth asym-IDAuth seq-ID
1X-RAY DIFFRACTION1chain 'A' and (resid 4 through 15 )A4 - 15
2X-RAY DIFFRACTION2chain 'A' and (resid 16 through 45 )A16 - 45
3X-RAY DIFFRACTION3chain 'A' and (resid 46 through 150 )A46 - 150
4X-RAY DIFFRACTION4chain 'A' and (resid 151 through 178 )A151 - 178
5X-RAY DIFFRACTION5chain 'A' and (resid 301 through 301 )A301
6X-RAY DIFFRACTION6chain 'B' and (resid 5 through 15 )B5 - 15
7X-RAY DIFFRACTION7chain 'B' and (resid 16 through 58 )B16 - 58
8X-RAY DIFFRACTION8chain 'B' and (resid 59 through 70 )B59 - 70
9X-RAY DIFFRACTION9chain 'B' and (resid 71 through 175 )B71 - 175
10X-RAY DIFFRACTION10chain 'C' and (resid 11 through 22 )C11 - 22
11X-RAY DIFFRACTION11chain 'C' and (resid 23 through 39 )C23 - 39
12X-RAY DIFFRACTION12chain 'C' and (resid 40 through 58 )C40 - 58
13X-RAY DIFFRACTION13chain 'C' and (resid 59 through 70 )C59 - 70
14X-RAY DIFFRACTION14chain 'C' and (resid 71 through 115 )C71 - 115
15X-RAY DIFFRACTION15chain 'C' and (resid 116 through 128 )C116 - 128
16X-RAY DIFFRACTION16chain 'C' and (resid 129 through 150 )C129 - 150
17X-RAY DIFFRACTION17chain 'C' and (resid 151 through 177 )C151 - 177
18X-RAY DIFFRACTION18chain 'C' and (resid 301 through 301 )C301
19X-RAY DIFFRACTION19chain 'D' and (resid 6 through 15 )D6 - 15
20X-RAY DIFFRACTION20chain 'D' and (resid 16 through 39 )D16 - 39
21X-RAY DIFFRACTION21chain 'D' and (resid 40 through 58 )D40 - 58
22X-RAY DIFFRACTION22chain 'D' and (resid 59 through 84 )D59 - 84
23X-RAY DIFFRACTION23chain 'D' and (resid 85 through 110 )D85 - 110
24X-RAY DIFFRACTION24chain 'D' and (resid 111 through 123 )D111 - 123
25X-RAY DIFFRACTION25chain 'D' and (resid 124 through 150 )D124 - 150
26X-RAY DIFFRACTION26chain 'D' and (resid 151 through 176 )D151 - 176
27X-RAY DIFFRACTION27chain 'E' and (resid 4 through 45 )E4 - 45
28X-RAY DIFFRACTION28chain 'E' and (resid 46 through 177 )E46 - 177
29X-RAY DIFFRACTION29chain 'F' and (resid 6 through 15 )F6 - 15
30X-RAY DIFFRACTION30chain 'F' and (resid 16 through 70 )F16 - 70
31X-RAY DIFFRACTION31chain 'F' and (resid 71 through 176 )F71 - 176

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