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Yorodumi- PDB-6mqu: PL5, synthetic transmembrane domain variant of human phospholamban -
+Open data
-Basic information
Entry | Database: PDB / ID: 6mqu | ||||||
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Title | PL5, synthetic transmembrane domain variant of human phospholamban | ||||||
Components | PL5, designed TM pentamer | ||||||
Keywords | DE NOVO PROTEIN / designed / engineered / transmembrane / pentamer / phospholamban | ||||||
Biological species | synthetic construct (others) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 3.17 Å | ||||||
Authors | Mravic, M. / Thomaston, J.L. / DeGrado, W.F. | ||||||
Funding support | United States, 1items
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Citation | Journal: Science / Year: 2019 Title: Packing of apolar side chains enables accurate design of highly stable membrane proteins. Authors: Mravic, M. / Thomaston, J.L. / Tucker, M. / Solomon, P.E. / Liu, L. / DeGrado, W.F. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 6mqu.cif.gz | 65.3 KB | Display | PDBx/mmCIF format |
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PDB format | pdb6mqu.ent.gz | 49.4 KB | Display | PDB format |
PDBx/mmJSON format | 6mqu.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 6mqu_validation.pdf.gz | 462.6 KB | Display | wwPDB validaton report |
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Full document | 6mqu_full_validation.pdf.gz | 465.7 KB | Display | |
Data in XML | 6mqu_validation.xml.gz | 10.6 KB | Display | |
Data in CIF | 6mqu_validation.cif.gz | 13.9 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/mq/6mqu ftp://data.pdbj.org/pub/pdb/validation_reports/mq/6mqu | HTTPS FTP |
-Related structure data
-Links
-Assembly
Deposited unit |
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1 |
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2 |
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Unit cell |
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-Components
#1: Protein/peptide | Mass: 3806.718 Da / Num. of mol.: 10 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others) #2: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 2.63 Å3/Da / Density % sol: 53.27 % |
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Crystal grow | Temperature: 298 K / Method: vapor diffusion, hanging drop Details: 12 MM C8E4, 20MM MGCL HEXAHYDRATE, 25MM NACL, 50 MM HEPES, 16% PEG400, VAPOR DIFFUSION, TEMPERATURE 298K |
-Data collection
Diffraction | Mean temperature: 100 K / Serial crystal experiment: N |
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Diffraction source | Source: SYNCHROTRON / Site: ALS / Beamline: 8.3.1 / Wavelength: 1.1158 Å |
Detector | Type: DECTRIS PILATUS3 S 6M / Detector: PIXEL / Date: May 21, 2017 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.1158 Å / Relative weight: 1 |
Reflection | Resolution: 3.17→87.84 Å / Num. obs: 6819 / % possible obs: 99 % / Redundancy: 3.9 % / Rmerge(I) obs: 0.171 / Net I/σ(I): 6.2 |
Reflection shell | Resolution: 3.17→3.39 Å / Redundancy: 3.9 % / Rmerge(I) obs: 0.54 / Mean I/σ(I) obs: 2.7 / Num. unique obs: 1212 / CC1/2: 0.802 / Rpim(I) all: 0.314 / % possible all: 98.2 |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT / Resolution: 3.17→87.84 Å / SU ML: 0.32 / Cross valid method: FREE R-VALUE / σ(F): 1.34 / Phase error: 25.52
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å | ||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 46.4 Å2 | ||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 3.17→87.84 Å
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Refine LS restraints |
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LS refinement shell |
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