+Open data
-Basic information
Entry | Database: PDB / ID: 5uhs | ||||||
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Title | Structure of a SemiSWEET D57A mutant | ||||||
Components | Sugar transporter SemiSWEET | ||||||
Keywords | TRANSPORT PROTEIN / membrane / transporter / SemiSWEET | ||||||
Function / homology | Function and homology information glucose transmembrane transporter activity / glucose transmembrane transport / protein homodimerization activity / membrane / plasma membrane Similarity search - Function | ||||||
Biological species | Leptospira biflexa serovar Patoc (bacteria) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 2.8 Å | ||||||
Authors | Fastman, N.M. / Feng, L. | ||||||
Citation | Journal: Cell / Year: 2017 Title: Mechanism of Substrate Translocation in an Alternating Access Transporter. Authors: Latorraca, N.R. / Fastman, N.M. / Venkatakrishnan, A.J. / Frommer, W.B. / Dror, R.O. / Feng, L. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 5uhs.cif.gz | 46.8 KB | Display | PDBx/mmCIF format |
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PDB format | pdb5uhs.ent.gz | 31.5 KB | Display | PDB format |
PDBx/mmJSON format | 5uhs.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/uh/5uhs ftp://data.pdbj.org/pub/pdb/validation_reports/uh/5uhs | HTTPS FTP |
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-Related structure data
Related structure data | 5uhqC 4qncS C: citing same article (ref.) S: Starting model for refinement |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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-Components
#1: Protein | Mass: 10680.742 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Leptospira biflexa serovar Patoc (strain Patoc 1 / ATCC 23582 / Paris) (bacteria) Strain: Patoc 1 / ATCC 23582 / Paris / Gene: LEPBI_I1613 / Production host: Escherichia coli (E. coli) / References: UniProt: B0SR19 #2: Chemical | #3: Sugar | ChemComp-BGC / | |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density % sol: 34.26 % |
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Crystal grow | Temperature: 293 K / Method: lipidic cubic phase Details: 11.7 MAG 50 mM tris pH 8 100 mM LiSO4 35% PEG 400 40 mM glucose |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: SYNCHROTRON / Site: APS / Beamline: 23-ID-D / Wavelength: 1.03327 Å |
Detector | Type: DECTRIS PILATUS3 6M / Detector: PIXEL / Date: Nov 19, 2014 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.03327 Å / Relative weight: 1 |
Reflection | Resolution: 2.8→50 Å / Num. obs: 4006 / % possible obs: 96.92 % / Redundancy: 9.3 % / Net I/σ(I): 13.73 |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: 4qnc Resolution: 2.8→29 Å / SU ML: 0.29 / Cross valid method: FREE R-VALUE / σ(F): 1.34 / Phase error: 30.11
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å | ||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2.8→29 Å
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Refine LS restraints |
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LS refinement shell |
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