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Open data
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Basic information
Entry | Database: PDB / ID: 6mj8 | ||||||
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Title | Structure of Candida glabrata Csm1:Mam1 complex | ||||||
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![]() | CELL CYCLE / monopolin / kinetochore | ||||||
Function / homology | ![]() microtubule site clamp / chromosome, centromeric core domain / monopolin complex / meiotic sister chromatid segregation / spindle attachment to meiosis I kinetochore / protein localization to nucleolar rDNA repeats / meiotic sister chromatid cohesion, centromeric / rDNA chromatin condensation / attachment of mitotic spindle microtubules to kinetochore / mitotic spindle ...microtubule site clamp / chromosome, centromeric core domain / monopolin complex / meiotic sister chromatid segregation / spindle attachment to meiosis I kinetochore / protein localization to nucleolar rDNA repeats / meiotic sister chromatid cohesion, centromeric / rDNA chromatin condensation / attachment of mitotic spindle microtubules to kinetochore / mitotic spindle / nuclear envelope / nucleolus / identical protein binding Similarity search - Function | ||||||
Biological species | ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Singh, N. / Corbett, K.D. | ||||||
Funding support | ![]()
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![]() | ![]() Title: The molecular basis of monopolin recruitment to the kinetochore. Authors: Plowman, R. / Singh, N. / Tromer, E.C. / Payan, A. / Duro, E. / Spanos, C. / Rappsilber, J. / Snel, B. / Kops, G.J.P.L. / Corbett, K.D. / Marston, A.L. | ||||||
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 111 KB | Display | ![]() |
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PDB format | ![]() | 83.9 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 445.4 KB | Display | ![]() |
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Full document | ![]() | 447.4 KB | Display | |
Data in XML | ![]() | 10.2 KB | Display | |
Data in CIF | ![]() | 13 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 6mjbC ![]() 6mjcC ![]() 6mjeC ![]() 3n4rS S: Starting model for refinement C: citing same article ( |
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Similar structure data | |
Experimental dataset #1 | Data reference: ![]() |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 13241.077 Da / Num. of mol.: 2 / Fragment: UNP residues 69-181 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() #2: Protein | Mass: 6611.495 Da / Num. of mol.: 2 / Fragment: UNP residues 162-216 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.03 Å3/Da / Density % sol: 39.49 % |
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Crystal grow | Temperature: 293 K / Method: vapor diffusion, hanging drop / pH: 6.5 Details: 0.1 M MES, pH 6.5, 0.6 M sodium chloride, 20% PEG4000, cryoprotectant: 20% PEG400 |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: ADSC QUANTUM 315 / Detector: CCD / Date: Dec 5, 2013 |
Radiation | Monochromator: Cryogenically-cooled single crystal Si(220) side bounce Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.97918 Å / Relative weight: 1 |
Reflection | Resolution: 3.03→55 Å / Num. obs: 6723 / % possible obs: 100 % / Redundancy: 6.9 % / Rmerge(I) obs: 0.308 / Rpim(I) all: 0.136 / Rrim(I) all: 0.358 / Net I/σ(I): 8.4 |
Reflection shell | Resolution: 3.03→3.2 Å / Rmerge(I) obs: 1.651 / Num. unique obs: 958 / Rpim(I) all: 0.714 / Rrim(I) all: 1.919 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: PDB entry 3N4R Resolution: 3.03→54.915 Å / SU ML: 0.1 / Cross valid method: FREE R-VALUE / σ(F): 1.34 / Phase error: 29.6
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å | ||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 3.03→54.915 Å
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Refine LS restraints |
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LS refinement shell |
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Refinement TLS params. | Method: refined / Origin x: 6.4084 Å / Origin y: 7.5734 Å / Origin z: 12.409 Å
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Refinement TLS group | Selection details: all |