+Open data
-Basic information
Entry | Database: PDB / ID: 6m6u | ||||||
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Title | Crystal structure the toxin-antitoxin MntA-HpeT mutant-D39ED41E | ||||||
Components |
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Keywords | ANTITOXIN / crystal structure of a unique toxin-antitoxin system | ||||||
Function / homology | Function and homology information protein adenylyltransferase / toxin-antitoxin complex / RNA nuclease activity / nucleotidyltransferase activity / endonuclease activity / Hydrolases; Acting on ester bonds / nucleotide binding / DNA binding / ATP binding / metal ion binding Similarity search - Function | ||||||
Biological species | Shewanella oneidensis MR-1 (bacteria) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 2.349 Å | ||||||
Authors | Ouyang, S.Y. / Zhen, X.K. | ||||||
Citation | Journal: Nucleic Acids Res. / Year: 2020 Title: Novel polyadenylylation-dependent neutralization mechanism of the HEPN/MNT toxin/antitoxin system. Authors: Yao, J. / Zhen, X. / Tang, K. / Liu, T. / Xu, X. / Chen, Z. / Guo, Y. / Liu, X. / Wood, T.K. / Ouyang, S. / Wang, X. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 6m6u.cif.gz | 408.1 KB | Display | PDBx/mmCIF format |
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PDB format | pdb6m6u.ent.gz | 338.9 KB | Display | PDB format |
PDBx/mmJSON format | 6m6u.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/m6/6m6u ftp://data.pdbj.org/pub/pdb/validation_reports/m6/6m6u | HTTPS FTP |
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-Related structure data
Related structure data | 6m6vC 6m6wC 7bxoC 5yepS S: Starting model for refinement C: citing same article (ref.) |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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-Components
#1: Protein | Mass: 15610.635 Da / Num. of mol.: 2 / Mutation: D39E, D41E Source method: isolated from a genetically manipulated source Source: (gene. exp.) Shewanella oneidensis MR-1 (bacteria) / Strain: MR-1 / Gene: SO_3165 / Production host: Escherichia coli (E. coli) / References: UniProt: Q8ECH7 #2: Protein | Mass: 15330.544 Da / Num. of mol.: 6 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Shewanella oneidensis MR-1 (bacteria) / Strain: MR-1 / Gene: SO_3166 / Production host: Escherichia coli (E. coli) / References: UniProt: Q8ECH6 |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 2.91 Å3/Da / Density % sol: 57.7 % |
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Crystal grow | Temperature: 293 K / Method: vapor diffusion, sitting drop / pH: 7 Details: 30%(v/v)Jeffamine M-600 PH7.0 100mM HEPES/Sodium hydroxide PH7.0 PH range: 5-9 |
-Data collection
Diffraction | Mean temperature: 100 K / Serial crystal experiment: N |
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Diffraction source | Source: SYNCHROTRON / Site: SSRF / Beamline: BL17U1 / Wavelength: 0.97982 Å |
Detector | Type: DECTRIS EIGER X 16M / Detector: PIXEL / Date: Oct 1, 2019 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.97982 Å / Relative weight: 1 |
Reflection | Resolution: 2.349→100.1 Å / Num. obs: 65138 / % possible obs: 99.74 % / Redundancy: 6.8 % / Biso Wilson estimate: 50.21 Å2 / CC1/2: 0.999 / Net I/σ(I): 16.6 |
Reflection shell | Resolution: 2.349→2.41 Å / Num. unique obs: 4450 / CC1/2: 0.744 |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: 5yep Resolution: 2.349→53.908 Å / SU ML: 0.3 / Cross valid method: THROUGHOUT / σ(F): 0 / Phase error: 28.3 / Stereochemistry target values: ML
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso max: 169.86 Å2 / Biso mean: 66.5052 Å2 / Biso min: 26.74 Å2 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: final / Resolution: 2.349→53.908 Å
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Refine LS restraints |
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LS refinement shell | Refine-ID: X-RAY DIFFRACTION / Rfactor Rfree error: 0
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Refinement TLS params. | Method: refined / Origin x: 20.0011 Å / Origin y: 9.053 Å / Origin z: 30.5326 Å
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Refinement TLS group |
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