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Open data
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Basic information
Entry | Database: PDB / ID: 6m34 | ||||||
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Title | Structural and functional insights into an Archaeal synthase | ||||||
![]() | Digeranylgeranylglyceryl phosphate synthase | ||||||
![]() | MEMBRANE PROTEIN / Lipid | ||||||
Function / homology | ![]() geranylgeranylglycerol-phosphate geranylgeranyltransferase / geranylgeranylglycerol-phosphate geranylgeranyltransferase activity / glycerophospholipid metabolic process / phospholipid biosynthetic process / magnesium ion binding / plasma membrane Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Wei, C. / Sixue, R. | ||||||
![]() | ![]() Title: Structural and mechanistic insights into the biosynthesis of Digeranylgeranylglyceryl phosphate synthase in membranes Authors: Wei, C. / Sixue, R. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 124.1 KB | Display | ![]() |
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PDB format | ![]() | 97 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 2.2 MB | Display | ![]() |
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Full document | ![]() | 2.2 MB | Display | |
Data in XML | ![]() | 22.1 KB | Display | |
Data in CIF | ![]() | 28.9 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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1 |
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Unit cell |
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Components
-Protein , 1 types, 2 molecules AB
#1: Protein | Mass: 31785.689 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() Strain: DSM 2661 / Gene: MJ0279 / Production host: ![]() ![]() References: UniProt: Q57727, geranylgeranylglycerol-phosphate geranylgeranyltransferase |
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-Non-polymers , 5 types, 10 molecules ![](data/chem/img/MPG.gif)
![](data/chem/img/DMA.gif)
![](data/chem/img/PO4.gif)
![](data/chem/img/MG.gif)
![](data/chem/img/LDA.gif)
![](data/chem/img/DMA.gif)
![](data/chem/img/PO4.gif)
![](data/chem/img/MG.gif)
![](data/chem/img/LDA.gif)
#2: Chemical | ChemComp-MPG / [( #3: Chemical | #4: Chemical | #5: Chemical | ChemComp-MG / | #6: Chemical | ChemComp-LDA / | |
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-Details
Has ligand of interest | Y |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.96 Å3/Da / Density % sol: 58.4 % |
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Crystal grow | Temperature: 293.15 K / Method: lipidic cubic phase Details: 200 mM NaCl, 20% (w/v) PEG400, and 200 mM Tris-HCl (pH 7.0). |
-Data collection
Diffraction | Mean temperature: 100 K / Serial crystal experiment: N | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Diffraction source | Source: ![]() ![]() ![]() | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Detector | Type: DECTRIS PILATUS3 R 1M / Detector: PIXEL / Date: Oct 25, 2018 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Radiation wavelength | Wavelength: 0.9793 Å / Relative weight: 1 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Reflection | Resolution: 2.89→39.91 Å / Num. obs: 16504 / % possible obs: 99 % / Redundancy: 7.025 % / Biso Wilson estimate: 62.73 Å2 / CC1/2: 0.995 / Rmerge(I) obs: 0.164 / Rrim(I) all: 0.178 / Χ2: 1.119 / Net I/σ(I): 9.65 / Num. measured all: 225465 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Reflection shell | Diffraction-ID: 1
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Processing
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Refinement | Method to determine structure: ![]() Details: SF FILE CONTAINS FRIEDEL PAIRS UNDER I/F_MINUS AND I/F_PLUS COLUMNS.
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Displacement parameters | Biso max: 139.39 Å2 / Biso mean: 58.08 Å2 / Biso min: 31.91 Å2
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Refine analyze | Luzzati coordinate error obs: 0.46 Å | ||||||||||||||||||||||||
Refinement step | Cycle: final / Resolution: 2.9→39.91 Å
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LS refinement shell | Resolution: 2.9→2.92 Å / Rfactor Rfree error: 0 / Total num. of bins used: 41
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