+Open data
-Basic information
Entry | Database: PDB / ID: 6m23 | ||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Title | Overall structure of KCC2 | ||||||||||||
Components | Solute carrier family 12 member 5 | ||||||||||||
Keywords | MEMBRANE PROTEIN / KCC2 | ||||||||||||
Function / homology | Function and homology information thermosensory behavior / intracellular pH reduction / potassium:chloride symporter activity / Cation-coupled Chloride cotransporters / hypotonic response / intracellular chloride ion homeostasis / chloride ion homeostasis / intracellular monoatomic ion homeostasis / dendritic spine development / ammonium channel activity ...thermosensory behavior / intracellular pH reduction / potassium:chloride symporter activity / Cation-coupled Chloride cotransporters / hypotonic response / intracellular chloride ion homeostasis / chloride ion homeostasis / intracellular monoatomic ion homeostasis / dendritic spine development / ammonium channel activity / potassium ion homeostasis / cell volume homeostasis / chloride transmembrane transporter activity / plasma membrane => GO:0005886 / potassium ion import across plasma membrane / monoatomic ion transport / chloride transmembrane transport / dendrite membrane / learning / cell periphery / multicellular organism growth / chemical synaptic transmission / perikaryon / membrane => GO:0016020 / response to xenobiotic stimulus / neuron projection / neuronal cell body / synapse / protein kinase binding / plasma membrane Similarity search - Function | ||||||||||||
Biological species | Homo sapiens (human) | ||||||||||||
Method | ELECTRON MICROSCOPY / single particle reconstruction / cryo EM / Resolution: 3.2 Å | ||||||||||||
Authors | Chi, X.M. / Li, X.R. / Chen, Y. / Zhang, Y.Y. / Su, Q. / Zhou, Q. | ||||||||||||
Funding support | China, 3items
| ||||||||||||
Citation | Journal: Cell Res / Year: 2021 Title: Cryo-EM structures of the full-length human KCC2 and KCC3 cation-chloride cotransporters. Authors: Ximin Chi / Xiaorong Li / Yun Chen / Yuanyuan Zhang / Qiang Su / Qiang Zhou / | ||||||||||||
History |
|
-Structure visualization
Movie |
Movie viewer |
---|---|
Structure viewer | Molecule: MolmilJmol/JSmol |
-Downloads & links
-Download
PDBx/mmCIF format | 6m23.cif.gz | 341.4 KB | Display | PDBx/mmCIF format |
---|---|---|---|---|
PDB format | pdb6m23.ent.gz | 274.4 KB | Display | PDB format |
PDBx/mmJSON format | 6m23.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 6m23_validation.pdf.gz | 1.2 MB | Display | wwPDB validaton report |
---|---|---|---|---|
Full document | 6m23_full_validation.pdf.gz | 1.2 MB | Display | |
Data in XML | 6m23_validation.xml.gz | 54.3 KB | Display | |
Data in CIF | 6m23_validation.cif.gz | 81.3 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/m2/6m23 ftp://data.pdbj.org/pub/pdb/validation_reports/m2/6m23 | HTTPS FTP |
-Related structure data
Related structure data | 30061MC 6m1yC 6m22C M: map data used to model this data C: citing same article (ref.) |
---|---|
Similar structure data |
-Links
-Assembly
Deposited unit |
|
---|---|
1 |
|
-Components
-Protein , 1 types, 2 molecules AB
#1: Protein | Mass: 125295.617 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Homo sapiens (human) / Gene: KCC2 / Cell line (production host): HEK293F / Production host: Homo sapiens (human) / References: UniProt: Q9H2X9 |
---|
-Sugars , 2 types, 8 molecules
#2: Polysaccharide | 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose Source method: isolated from a genetically manipulated source #5: Sugar | |
---|
-Non-polymers , 3 types, 6 molecules
#3: Chemical | #4: Chemical | #6: Water | ChemComp-HOH / | |
---|
-Details
Has ligand of interest | N |
---|
-Experimental details
-Experiment
Experiment | Method: ELECTRON MICROSCOPY |
---|---|
EM experiment | Aggregation state: PARTICLE / 3D reconstruction method: single particle reconstruction |
-Sample preparation
Component | Name: KCC2 / Type: COMPLEX / Entity ID: #1 / Source: RECOMBINANT |
---|---|
Source (natural) | Organism: Homo sapiens (human) |
Source (recombinant) | Organism: Homo sapiens (human) / Cell: HEK293F |
Buffer solution | pH: 7.4 |
Specimen | Embedding applied: NO / Shadowing applied: NO / Staining applied: NO / Vitrification applied: YES |
Vitrification | Cryogen name: ETHANE |
-Electron microscopy imaging
Experimental equipment | Model: Titan Krios / Image courtesy: FEI Company |
---|---|
Microscopy | Model: FEI TITAN KRIOS |
Electron gun | Electron source: FIELD EMISSION GUN / Accelerating voltage: 300 kV / Illumination mode: FLOOD BEAM |
Electron lens | Mode: BRIGHT FIELD / Alignment procedure: COMA FREE |
Specimen holder | Cryogen: NITROGEN / Specimen holder model: FEI TITAN KRIOS AUTOGRID HOLDER |
Image recording | Electron dose: 50 e/Å2 / Film or detector model: GATAN K3 BIOQUANTUM (6k x 4k) |
-Processing
EM software | Name: RELION / Version: 3.0.6 / Category: 3D reconstruction |
---|---|
CTF correction | Type: PHASE FLIPPING AND AMPLITUDE CORRECTION |
3D reconstruction | Resolution: 3.2 Å / Resolution method: FSC 0.143 CUT-OFF / Num. of particles: 186236 / Symmetry type: POINT |