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- PDB-6lgg: Bombyx mori GH13 sucrose hydrolase mutant E322Q complexed with sucrose -
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Open data
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Basic information
Entry | Database: PDB / ID: 6lgg | ||||||||||||
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Title | Bombyx mori GH13 sucrose hydrolase mutant E322Q complexed with sucrose | ||||||||||||
![]() | Sucrose hydrolase | ||||||||||||
![]() | HYDROLASE / Sucrose / Glycoside hydrolase / GH13 | ||||||||||||
Function / homology | ![]() alpha-glucosidase / hydrolase activity, acting on glycosyl bonds / carbohydrate metabolic process / hydrolase activity / metal ion binding Similarity search - Function | ||||||||||||
Biological species | ![]() ![]() | ||||||||||||
Method | ![]() ![]() ![]() | ||||||||||||
![]() | Miyazaki, T. | ||||||||||||
Funding support | ![]()
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![]() | ![]() Title: Structure-function analysis of silkworm sucrose hydrolase uncovers the mechanism of substrate specificity in GH13 subfamily 17exo-alpha-glucosidases. Authors: Miyazaki, T. / Park, E.Y. | ||||||||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 262.7 KB | Display | ![]() |
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PDB format | ![]() | 204.8 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 3.6 MB | Display | ![]() |
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Full document | ![]() | 3.6 MB | Display | |
Data in XML | ![]() | 46.4 KB | Display | |
Data in CIF | ![]() | 70 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 6lgaC ![]() 6lgbC ![]() 6lgcC ![]() 6lgdC ![]() 6lgeC ![]() 6lgfC ![]() 6lghC ![]() 6lgiC ![]() 5brqS S: Starting model for refinement C: citing same article ( |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 68738.219 Da / Num. of mol.: 2 / Mutation: E322Q Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() #2: Polysaccharide | #3: Chemical | ChemComp-MG / #4: Chemical | #5: Water | ChemComp-HOH / | Has ligand of interest | Y | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.32 Å3/Da / Density % sol: 47.03 % |
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Crystal grow | Temperature: 293 K / Method: vapor diffusion, hanging drop Details: 8% PEG 3350, 0.2 M magnesium acetate, 10 mM sucrose |
-Data collection
Diffraction | Mean temperature: 100 K / Serial crystal experiment: N |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: DECTRIS PILATUS3 S 6M / Detector: PIXEL / Date: Nov 3, 2019 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1 Å / Relative weight: 1 |
Reflection | Resolution: 1.84→50 Å / Num. obs: 111648 / % possible obs: 99.9 % / Redundancy: 6.5 % / CC1/2: 0.998 / Rmerge(I) obs: 0.097 / Net I/σ(I): 12.4 |
Reflection shell | Resolution: 1.84→1.94 Å / Redundancy: 6.7 % / Rmerge(I) obs: 0.775 / Mean I/σ(I) obs: 2.4 / Num. unique obs: 16116 / CC1/2: 0.769 / % possible all: 100 |
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Processing
Software |
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Refinement | Method to determine structure: ![]() Starting model: 5BRQ Resolution: 1.84→49.28 Å / Cor.coef. Fo:Fc: 0.961 / Cor.coef. Fo:Fc free: 0.951 / Cross valid method: THROUGHOUT / σ(F): 0 / ESU R: 0.133 / ESU R Free: 0.122 Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS U VALUES : REFINED INDIVIDUALLY
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Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso max: 117.41 Å2 / Biso mean: 26.004 Å2 / Biso min: 14.14 Å2
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Refinement step | Cycle: final / Resolution: 1.84→49.28 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 1.84→1.888 Å / Rfactor Rfree error: 0 / Total num. of bins used: 20
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