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- PDB-6lc1: Structural basis of NR4A1 bound to the human pituitary proopiomel... -

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Basic information

Entry
Database: PDB / ID: 6lc1
TitleStructural basis of NR4A1 bound to the human pituitary proopiomelanocortin gene promoter
Components
  • DNA
  • DNA (25-MER)
  • Nuclear receptor subfamily 4 group A member 1
KeywordsDNA BINDING PROTEIN/DNA / nuclear receptor / DNA BINDING PROTEIN / DNA BINDING PROTEIN-DNA complex
Function / homology
Function and homology information


cellular response to corticotropin-releasing hormone stimulus / regulation of type B pancreatic cell proliferation / non-canonical inflammasome complex assembly / detection of lipopolysaccharide / AKT phosphorylates targets in the nucleus / endothelial cell chemotaxis / nuclear glucocorticoid receptor binding / macrophage activation / cell migration involved in sprouting angiogenesis / Constitutive Signaling by AKT1 E17K in Cancer ...cellular response to corticotropin-releasing hormone stimulus / regulation of type B pancreatic cell proliferation / non-canonical inflammasome complex assembly / detection of lipopolysaccharide / AKT phosphorylates targets in the nucleus / endothelial cell chemotaxis / nuclear glucocorticoid receptor binding / macrophage activation / cell migration involved in sprouting angiogenesis / Constitutive Signaling by AKT1 E17K in Cancer / fat cell differentiation / negative regulation of cell cycle / skeletal muscle cell differentiation / cellular response to vascular endothelial growth factor stimulus / cellular response to fibroblast growth factor stimulus / positive regulation of endothelial cell proliferation / lipopolysaccharide binding / Nuclear Receptor transcription pathway / nuclear receptor activity / fibrillar center / protein import into nucleus / sequence-specific double-stranded DNA binding / DNA-binding transcription activator activity, RNA polymerase II-specific / nuclear membrane / transcription regulator complex / transcription by RNA polymerase II / DNA-binding transcription factor activity, RNA polymerase II-specific / nuclear speck / positive regulation of apoptotic process / RNA polymerase II cis-regulatory region sequence-specific DNA binding / inflammatory response / protein heterodimerization activity / apoptotic process / regulation of transcription by RNA polymerase II / chromatin / signal transduction / positive regulation of transcription by RNA polymerase II / mitochondrion / DNA binding / zinc ion binding / nucleoplasm / identical protein binding / nucleus / cytosol
Similarity search - Function
Orphan nuclear receptor, HMR type / Orphan nuclear receptor / Erythroid Transcription Factor GATA-1, subunit A / Erythroid Transcription Factor GATA-1; Chain A / Nuclear hormone receptor / Nuclear hormones receptors DNA-binding region signature. / Zinc finger, nuclear hormone receptor-type / Double treble clef zinc finger, C4 type / Nuclear hormone receptors DNA-binding domain profile. / c4 zinc finger in nuclear hormone receptors ...Orphan nuclear receptor, HMR type / Orphan nuclear receptor / Erythroid Transcription Factor GATA-1, subunit A / Erythroid Transcription Factor GATA-1; Chain A / Nuclear hormone receptor / Nuclear hormones receptors DNA-binding region signature. / Zinc finger, nuclear hormone receptor-type / Double treble clef zinc finger, C4 type / Nuclear hormone receptors DNA-binding domain profile. / c4 zinc finger in nuclear hormone receptors / Nuclear hormone receptor, ligand-binding domain / Nuclear hormone receptor-like domain superfamily / Ligand-binding domain of nuclear hormone receptor / Nuclear receptor (NR) ligand-binding (LBD) domain profile. / Ligand binding domain of hormone receptors / Zinc finger, NHR/GATA-type / 2-Layer Sandwich / Alpha Beta
Similarity search - Domain/homology
DNA / DNA (> 10) / Nuclear receptor subfamily 4immunitygroup A member 1
Similarity search - Component
Biological speciesHomo sapiens (human)
MethodX-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 3.12 Å
AuthorsJiang, L. / Chen, Y.
Funding support China, 2items
OrganizationGrant numberCountry
National Natural Science Foundation of China81372904 and 81570537 China
National Natural Science Foundation of China81272971 China
CitationJournal: Biochem.Biophys.Res.Commun. / Year: 2020
Title: Structural basis of NR4A1 bound to the human pituitary proopiomelanocortin gene promoter.
Authors: Jiang, L. / Wei, H. / Yan, N. / Dai, S. / Li, J. / Qu, L. / Chen, X. / Guo, M. / Chen, Z. / Chen, Y.
History
DepositionNov 16, 2019Deposition site: PDBJ / Processing site: PDBJ
Revision 1.0Dec 18, 2019Provider: repository / Type: Initial release
Revision 1.1Dec 25, 2019Group: Database references / Derived calculations
Category: citation / pdbx_struct_assembly_gen / pdbx_struct_assembly_prop
Item: _citation.pdbx_database_id_PubMed / _pdbx_struct_assembly_gen.asym_id_list / _pdbx_struct_assembly_prop.value
Revision 1.2Feb 19, 2020Group: Database references / Category: citation
Item: _citation.journal_volume / _citation.page_first ..._citation.journal_volume / _citation.page_first / _citation.page_last / _citation.year
Revision 1.3Nov 22, 2023Group: Data collection / Database references ...Data collection / Database references / Derived calculations / Refinement description
Category: chem_comp_atom / chem_comp_bond ...chem_comp_atom / chem_comp_bond / database_2 / pdbx_initial_refinement_model / struct_conn / struct_ncs_dom_lim
Item: _database_2.pdbx_DOI / _database_2.pdbx_database_accession ..._database_2.pdbx_DOI / _database_2.pdbx_database_accession / _struct_conn.pdbx_dist_value / _struct_ncs_dom_lim.beg_auth_comp_id / _struct_ncs_dom_lim.beg_label_asym_id / _struct_ncs_dom_lim.beg_label_comp_id / _struct_ncs_dom_lim.beg_label_seq_id / _struct_ncs_dom_lim.end_auth_comp_id / _struct_ncs_dom_lim.end_label_asym_id / _struct_ncs_dom_lim.end_label_comp_id / _struct_ncs_dom_lim.end_label_seq_id

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Assembly

Deposited unit
I: DNA
J: Nuclear receptor subfamily 4 group A member 1
L: DNA (25-MER)
A: Nuclear receptor subfamily 4 group A member 1
C: DNA (25-MER)
D: Nuclear receptor subfamily 4 group A member 1
F: DNA
G: Nuclear receptor subfamily 4 group A member 1
hetero molecules


Theoretical massNumber of molelcules
Total (without water)72,06916
Polymers71,5458
Non-polymers5238
Water00
1
I: DNA
J: Nuclear receptor subfamily 4 group A member 1
L: DNA (25-MER)
G: Nuclear receptor subfamily 4 group A member 1
hetero molecules


Theoretical massNumber of molelcules
Total (without water)36,0348
Polymers35,7734
Non-polymers2624
Water0
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area7160 Å2
ΔGint-36 kcal/mol
Surface area16840 Å2
MethodPISA
2
A: Nuclear receptor subfamily 4 group A member 1
C: DNA (25-MER)
D: Nuclear receptor subfamily 4 group A member 1
F: DNA
hetero molecules


Theoretical massNumber of molelcules
Total (without water)36,0348
Polymers35,7734
Non-polymers2624
Water0
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area7330 Å2
ΔGint-110 kcal/mol
Surface area17330 Å2
MethodPISA
Unit cell
Length a, b, c (Å)36.822, 63.210, 91.762
Angle α, β, γ (deg.)109.980, 90.210, 89.540
Int Tables number1
Space group name H-MP1
Noncrystallographic symmetry (NCS)NCS domain:
IDEns-IDDetails
11(chain F and (resid 2 through 11 or resid 13 through 26))
21(chain I and (resid 2 through 11 or resid 13 through 26))

NCS domain segments:

Ens-ID: 1

Dom-IDComponent-IDBeg auth comp-IDBeg label comp-IDEnd auth comp-IDEnd label comp-IDSelection detailsAuth asym-IDLabel asym-IDAuth seq-IDLabel seq-ID
11DGDGDADA(chain F and (resid 2 through 11 or resid 13 through 26))FG2 - 112 - 11
12DCDCDGDG(chain F and (resid 2 through 11 or resid 13 through 26))FG13 - 2613 - 26
21DGDGDADA(chain I and (resid 2 through 11 or resid 13 through 26))IA2 - 112 - 11
22DCDCDGDG(chain I and (resid 2 through 11 or resid 13 through 26))IA13 - 2613 - 26

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Components

#1: DNA chain DNA


Mass: 7971.166 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human) / Production host: Escherichia coli (E. coli)
#2: Protein
Nuclear receptor subfamily 4 group A member 1 / Early response protein NAK1 / Nuclear hormone receptor NUR/77 / Nur77 / Orphan nuclear receptor HMR ...Early response protein NAK1 / Nuclear hormone receptor NUR/77 / Nur77 / Orphan nuclear receptor HMR / Orphan nuclear receptor TR3 / ST-59 / Testicular receptor 3


Mass: 9899.692 Da / Num. of mol.: 4
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human) / Gene: NR4A1, GFRP1, HMR, NAK1 / Production host: Escherichia coli (E. coli) / References: UniProt: P22736
#3: DNA chain DNA (25-MER)


Mass: 8002.178 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human) / Production host: Escherichia coli (E. coli)
#4: Chemical
ChemComp-ZN / ZINC ION


Mass: 65.409 Da / Num. of mol.: 8 / Source method: obtained synthetically / Formula: Zn
Has ligand of interestN

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Experimental details

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Experiment

ExperimentMethod: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

CrystalDensity Matthews: 2.84 Å3/Da / Density % sol: 56.76 %
Crystal growTemperature: 291 K / Method: vapor diffusion, hanging drop
Details: 100 mM Ammonium sulfate, 10mM magnesium chloride, 50mM MES monohydrate pH 5.6, 20% w/v Polyethylene glycol 8K

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Data collection

DiffractionMean temperature: 100 K / Serial crystal experiment: N
Diffraction sourceSource: SYNCHROTRON / Site: SSRF / Beamline: BL17U1 / Wavelength: 0.97915 Å
DetectorType: DECTRIS EIGER X 16M / Detector: PIXEL / Date: May 27, 2015
RadiationProtocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelengthWavelength: 0.97915 Å / Relative weight: 1
ReflectionResolution: 3.12→29.646 Å / Num. obs: 12751 / % possible obs: 92.1 % / Redundancy: 3.8 % / Rpim(I) all: 0.071 / Rrim(I) all: 0.136 / Net I/σ(I): 7
Reflection shellResolution: 3.12→3.232 Å / Mean I/σ(I) obs: 2.54 / Num. unique obs: 984 / Rpim(I) all: 0.181 / Rrim(I) all: 0.357

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Processing

Software
NameVersionClassification
PHENIX1.11.1_2575refinement
HKL-2000data reduction
HKL-2000data scaling
PHENIXphasing
RefinementMethod to determine structure: MOLECULAR REPLACEMENT
Starting model: 1cit

1cit


Resolution: 3.12→29.646 Å / SU ML: 0.45 / Cross valid method: THROUGHOUT / σ(F): 2.02 / Phase error: 30.86 / Stereochemistry target values: ML
RfactorNum. reflection% reflection
Rfree0.2676 677 5.31 %
Rwork0.2093 12072 -
obs0.2125 12749 92.22 %
Solvent computationShrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL
Displacement parametersBiso max: 158.76 Å2 / Biso mean: 93.8968 Å2 / Biso min: 20 Å2
Refinement stepCycle: final / Resolution: 3.12→29.646 Å
ProteinNucleic acidLigandSolventTotal
Num. atoms2681 2090 8 0 4779
Biso mean--92.65 --
Num. residues----446
Refine LS restraints
Refine-IDTypeDev idealNumber
X-RAY DIFFRACTIONf_bond_d0.0035065
X-RAY DIFFRACTIONf_angle_d0.737224
X-RAY DIFFRACTIONf_chiral_restr0.047786
X-RAY DIFFRACTIONf_plane_restr0.002578
X-RAY DIFFRACTIONf_dihedral_angle_d19.6912710
Refine LS restraints NCS
Ens-IDDom-IDAuth asym-IDNumberRefine-IDRmsType
11F468X-RAY DIFFRACTION17.4TORSIONAL
12I468X-RAY DIFFRACTION17.4TORSIONAL
LS refinement shell

Refine-ID: X-RAY DIFFRACTION / Rfactor Rfree error: 0

Resolution (Å)Rfactor RfreeNum. reflection RfreeRfactor RworkNum. reflection Rwork% reflection obs (%)
3.1202-3.36080.38261140.3047213381
3.3608-3.69850.31861420.2843235190
3.6985-4.23230.33921270.2462251697
4.2323-5.32720.24781550.1953257999
5.3272-29.6460.2081390.1582249396
Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

IDL11 (°2)L12 (°2)L13 (°2)L22 (°2)L23 (°2)L33 (°2)S11 (Å °)S12 (Å °)S13 (Å °)S21 (Å °)S22 (Å °)S23 (Å °)S31 (Å °)S32 (Å °)S33 (Å °)T11 (Å2)T12 (Å2)T13 (Å2)T22 (Å2)T23 (Å2)T33 (Å2)Origin x (Å)Origin y (Å)Origin z (Å)
19.0712-0.5854-2.0558.5682-4.78676.37361.3921-1.49371.86460.1353-2.3769-0.40740.533.0088-0.64291.0206-0.22750.18511.2899-0.12960.6823-38.856142.075941.2674
20.6558-0.85260.19661.8876-2.20144.02480.50270.56020.2545-0.65590.4617-0.1584-0.10370.5305-0.33131.3099-0.14440.29860.9314-0.04581.0701-32.794841.63416.4195
32.3919-0.8619-0.75825.0186-0.78755.5293-0.03540.4765-0.12290.3365-0.0790.81921.0156-0.7064-0.23750.9168-0.14270.24270.92580.0280.828-30.841542.2831-17.9641
44.345-0.1718-1.17481.1613-1.20523.6388-0.4073-0.6101-0.68781.07040.31890.8949-1.73140.4214-0.35351.245-0.11130.11640.78250.09930.8297-24.68157.9266-17.6609
53.64672.02020.80483.3839-0.39314.7863-0.0949-1.82690.54250.285-0.36941.2996-1.26410.6347-0.29751.0496-0.1730.16270.90420.08170.8694-21.355751.2314-13.1968
66.44640.0046-0.1316.3812-0.83216.5849-0.6914-0.1055-0.5590.88940.09150.52230.6710.9758-0.30670.5843-0.02310.01050.82410.03690.7701-20.992143.6971-22.0199
72.1121.8614-1.08451.6347-1.04914.3614-0.06911.30610.9580.5115-0.4343-0.1945-1.07660.4043-0.32850.8116-0.20060.05411.01030.07810.8964-17.419553.6514-31.9958
83.7966-0.481-0.11082.31410.30151.27170.04430.77770.2563-0.24230.62280.1708-0.28711.9033-0.35880.5351-0.14120.0241.2687-0.11230.5873-14.034551.456-25.3721
97.97873.39370.15712.8112.22423.406-0.0734-0.4192-0.164-0.43360.2741-0.76230.33441.9794-0.79260.6542-0.0245-0.3441.7096-0.57330.9069-12.372245.4556-15.6054
100.13990.4354-0.23451.4059-0.07733.4432-0.51870.1879-1.98511.0936-0.3091-2.42311.80581.5617-0.21381.06160.1730.19571.12990.17161.1759-18.077833.8387-12.8499
119.475.94747.79133.73694.88196.3536-2.3943-1.41081.50380.1209-0.73230.6929-0.0658-0.0957-1.48931.18710.34970.45011.14280.211.3466-23.109638.0537-5.1037
126.93351.64480.99165.5541-1.22093.9819-0.1859-0.5159-0.65490.019-0.17480.52381.5548-0.035-0.24730.9007-0.09140.21260.7539-0.05430.7352-28.825343.8318-27.7851
131.65010.0028-1.52780.8691.0315.76470.2384-0.70810.17251.0733-0.00120.3515-0.22310.3039-0.30471.348-0.19640.24430.93820.07720.8829-30.592542.2356-1.8768
143.3374-0.1258-2.05852.71493.15484.53140.4598-0.19830.6985-0.2470.5269-0.1844-0.84740.9322-0.28310.9185-0.16670.10080.7899-0.12560.8214-34.256141.611629.4704
151.92781.25070.0931.48390.83050.76-0.0162-0.65850.09660.8930.19510.9917-0.39820.0976-0.39010.95960.0752-0.10820.71020.1280.8734-48.5139.6995-14.0017
163.9646-1.35230.08362.153-0.86262.56050.73560.5644-0.94930.1046-0.2979-0.87910.3162-0.4572-0.34380.57380.108-0.08230.63480.00930.8003-43.780415.7228-18.9297
171.72220.96160.59011.34170.51610.42351.69530.6599-0.3994-0.3768-0.72810.5640.7156-0.9159-0.32570.71710.1658-0.05141.31610.09740.8853-46.43523.916-29.638
182.7148-1.2242-1.64820.57260.76041.01161.50681.6063-1.3269-0.426-0.2338-0.54281.2867-0.0495-0.13750.77370.034-0.42151.1922-0.23820.9191-54.1912.748-29.725
192.58660.39651.73774.23140.55381.24880.5684-0.1532-0.13010.0320.50320.8990.1066-0.5715-0.3540.60090.2238-0.02780.92730.06540.8556-53.02918.834-21.376
204.85454.43-3.1047.7466-4.80963.44490.92870.71541.44161.24120.92131.2821-1.1923-0.62671.37330.97050.15970.14230.04-0.4190.6918-42.122125.1452-13.6056
214.1038-0.6651-3.02131.8948-0.93767.82860.5764-1.3391-0.33671.7216-0.4794-0.3877-0.34991.7222-0.44260.84240.0185-0.15751.091-0.18531.0738-33.441522.2036-7.3647
223.4348-0.49690.47945.42372.51524.50230.4536-0.1935-0.4680.69830.4504-0.4512-0.1050.2385-0.30560.73950.1781-0.13990.7714-0.07260.8015-34.376811.9635-13.9434
233.59350.12281.79972.9098-1.16885.30530.13910.6677-0.0011-0.93730.0380.9585-0.6822-0.5512-0.1941.06070.0165-0.16420.7577-0.0110.8837-30.482410.720526.2558
241.6684-0.32111.31690.0421-0.17931.0546-1.2475-0.45240.5083-0.09050.79910.33221.6516-0.3632-0.37010.96530.1023-0.051.01280.08951.0234-24.3132-4.872730.9182
254.7476-0.8955-1.17622.9306-0.18453.9058-0.2013-0.3689-0.5247-0.4706-0.1235-0.07830.62731.324-0.35730.5530.0894-0.1170.77020.15880.7724-18.34454.42837.3045
263.6742.544-1.2615.94310.77041.0972-0.0079-1.2867-1.93320.01330.5923-0.08471.3678-1.9806-0.36861.05150.075-0.06710.93770.11221.1066-23.568-3.266244.3878
273.14440.75410.64990.8035-0.10751.3162-0.53460.22671.0428-0.28930.86030.33371.06941.8101-0.28740.53140.0227-0.0590.93750.08890.7522-14.06121.766738.8415
284.6241-2.45311.94711.4235-0.8063.94870.29020.8181.2647-0.10870.3022-1.16980.08941.9683-0.17660.7018-0.1941-0.03331.4094-0.02640.8852-13.09078.695728.8528
293.573-1.6321-1.03881.9689-0.94443.5784-0.3529-0.06580.8479-1.5054-0.3091-0.1975-2.06160.7304-0.28661.19-0.0708-0.16980.86040.16231.0566-20.635218.424623.7689
304.1006-0.51541.58113.379-2.75584.6950.2219-0.13090.189-0.4865-0.08070.2451-0.43660.0028-0.32190.9170.0511-0.19070.6912-0.05680.9226-30.27610.242924.8745
313.3740.43761.28532.47121.40863.78970.66430.5104-0.45180.1960.4286-0.42571.27860.5861-0.2770.9450.1331-0.19470.8548-0.16470.7981-34.389410.857-17.8825
322.77831.0540.27953.72030.62570.09160.665-0.52171.7076-0.3953-0.71411.247-0.06370.4352-0.42440.9839-0.11750.14850.80360.1281.2427-50.697146.251929.1692
334.01161.11530.73332.18050.56523.83630.548-0.07850.4056-0.3732-0.3407-0.5015-0.59211.2857-0.33430.75360.00170.17310.5891-0.03440.7636-44.377837.40330.3993
340.82360.24950.63821.0578-0.15822.19460.5971-0.72710.2801-0.0648-0.5921.5108-0.0017-0.3504-0.37511.171-0.105-0.01071.02590.11791.2688-53.832932.855843.8873
352.77630.10770.19751.6290.7470.4880.4642-1.03041.03660.4267-0.5487-0.012-0.8332-1.2201-0.41081.472-0.09170.23851.3104-0.45411.2354-52.589644.960241.5542
363.0029-0.97380.36873.2076-0.08282.83620.42450.73230.61550.2550.77390.85090.78620.4849-0.36460.8308-0.15530.07710.8330.0620.9695-52.978334.100637.3328
374.1942-0.6483-0.61770.4057-0.64481.40830.09442.0731-2.1667-0.4422-0.42870.35990.7752-0.2138-0.31980.7232-0.0121-0.1410.5529-0.14020.8281-42.222228.006427.0593
382.1666-1.09610.47961.9828-2.28154.261.42281.654-0.4689-1.1893-0.51980.20911.26091.4565-0.3441.1938-0.01810.10831.00360.12040.919-33.355231.233420.6478
Refinement TLS group
IDRefine-IDRefine TLS-IDSelection detailsAuth asym-IDAuth seq-ID
1X-RAY DIFFRACTION1chain 'I' and (resid 1 through 5 )I1 - 5
2X-RAY DIFFRACTION2chain 'I' and (resid 6 through 15 )I6 - 15
3X-RAY DIFFRACTION3chain 'I' and (resid 16 through 26 )I16 - 26
4X-RAY DIFFRACTION4chain 'J' and (resid 265 through 275 )J265 - 275
5X-RAY DIFFRACTION5chain 'J' and (resid 276 through 284 )J276 - 284
6X-RAY DIFFRACTION6chain 'J' and (resid 285 through 297 )J285 - 297
7X-RAY DIFFRACTION7chain 'J' and (resid 298 through 319 )J298 - 319
8X-RAY DIFFRACTION8chain 'J' and (resid 320 through 330 )J320 - 330
9X-RAY DIFFRACTION9chain 'J' and (resid 331 through 335 )J331 - 335
10X-RAY DIFFRACTION10chain 'J' and (resid 336 through 345 )J336 - 345
11X-RAY DIFFRACTION11chain 'J' and (resid 346 through 350 )J346 - 350
12X-RAY DIFFRACTION12chain 'L' and (resid 1 through 5 )L1 - 5
13X-RAY DIFFRACTION13chain 'L' and (resid 6 through 15 )L6 - 15
14X-RAY DIFFRACTION14chain 'L' and (resid 16 through 25 )L16 - 25
15X-RAY DIFFRACTION15chain 'A' and (resid 266 through 280 )A266 - 280
16X-RAY DIFFRACTION16chain 'A' and (resid 281 through 296 )A281 - 296
17X-RAY DIFFRACTION17chain 'A' and (resid 297 through 303 )A297 - 303
18X-RAY DIFFRACTION18chain 'A' and (resid 304 through 321 )A304 - 321
19X-RAY DIFFRACTION19chain 'A' and (resid 322 through 331 )A322 - 331
20X-RAY DIFFRACTION20chain 'A' and (resid 332 through 342 )A332 - 342
21X-RAY DIFFRACTION21chain 'A' and (resid 343 through 351 )A343 - 351
22X-RAY DIFFRACTION22chain 'C' and (resid 1 through 15 )C1 - 15
23X-RAY DIFFRACTION23chain 'C' and (resid 16 through 25 )C16 - 25
24X-RAY DIFFRACTION24chain 'D' and (resid 265 through 275 )D265 - 275
25X-RAY DIFFRACTION25chain 'D' and (resid 276 through 312 )D276 - 312
26X-RAY DIFFRACTION26chain 'D' and (resid 313 through 319 )D313 - 319
27X-RAY DIFFRACTION27chain 'D' and (resid 320 through 330 )D320 - 330
28X-RAY DIFFRACTION28chain 'D' and (resid 331 through 336 )D331 - 336
29X-RAY DIFFRACTION29chain 'D' and (resid 337 through 350 )D337 - 350
30X-RAY DIFFRACTION30chain 'F' and (resid 1 through 15 )F1 - 15
31X-RAY DIFFRACTION31chain 'F' and (resid 16 through 26 )F16 - 26
32X-RAY DIFFRACTION32chain 'G' and (resid 266 through 275 )G266 - 275
33X-RAY DIFFRACTION33chain 'G' and (resid 276 through 296 )G276 - 296
34X-RAY DIFFRACTION34chain 'G' and (resid 297 through 311 )G297 - 311
35X-RAY DIFFRACTION35chain 'G' and (resid 312 through 319 )G312 - 319
36X-RAY DIFFRACTION36chain 'G' and (resid 320 through 329 )G320 - 329
37X-RAY DIFFRACTION37chain 'G' and (resid 330 through 342 )G330 - 342
38X-RAY DIFFRACTION38chain 'G' and (resid 343 through 351 )G343 - 351

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