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- PDB-6l7m: Characterization and structural analysis of a thermostable zearal... -
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Open data
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Basic information
Entry | Database: PDB / ID: 6l7m | ||||||
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Title | Characterization and structural analysis of a thermostable zearalenone-degrading enzyme | ||||||
![]() | AB hydrolase-1 domain-containing protein | ||||||
![]() | HYDROLASE / mycotoxin / ZEN-degrading enzyme ZhdF / thermostablity | ||||||
Function / homology | alpha/beta hydrolase fold / Alpha/beta hydrolase fold-1 / Alpha/Beta hydrolase fold / AB hydrolase-1 domain-containing protein![]() | ||||||
Biological species | ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Wei, X.H. / Meng, G. | ||||||
![]() | ![]() Title: Characterization and structural analysis of a thermostable zearalenone-degrading enzyme Authors: Wei, X.H. / Meng, G. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 226.6 KB | Display | ![]() |
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PDB format | ![]() | 181.2 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 463.6 KB | Display | ![]() |
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Full document | ![]() | 510.4 KB | Display | |
Data in XML | ![]() | 51.1 KB | Display | |
Data in CIF | ![]() | 70.9 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 5xo6S S: Starting model for refinement |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 29807.023 Da / Num. of mol.: 4 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() #2: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 3.05 Å3/Da / Density % sol: 59.65 % |
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Crystal grow | Temperature: 281 K / Method: vapor diffusion, sitting drop / pH: 8 / Details: 20% PEG 3350 and 0.2 M Potassium fluoride |
-Data collection
Diffraction | Mean temperature: 100 K / Serial crystal experiment: N |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: ADSC QUANTUM 315r / Detector: CCD / Date: Apr 16, 2019 |
Radiation | Monochromator: NI FILTER / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.97931 Å / Relative weight: 1 |
Reflection | Resolution: 2.4→50 Å / Num. obs: 57727 / % possible obs: 100 % / Redundancy: 12.3 % / Biso Wilson estimate: 20.93 Å2 / CC1/2: 0.993 / Rmerge(I) obs: 0.2 / Rpim(I) all: 0.105 / Rrim(I) all: 0.268 / Χ2: 1.01 / Net I/av σ(I): 12.8 / Net I/σ(I): 12.8 |
Reflection shell | Resolution: 2.4→2.47 Å / Redundancy: 13 % / Rmerge(I) obs: 0.733 / Mean I/σ(I) obs: 5.9 / Num. unique obs: 4442 / Rpim(I) all: 0.657 / % possible all: 100 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: 5XO6 Resolution: 2.4→49.794 Å / Cor.coef. Fo:Fc: 0.868 / Cor.coef. Fo:Fc free: 0.844 / SU B: 0.001 / SU ML: 0 / Cross valid method: NONE / ESU R: 0.245 / ESU R Free: 0.272 Details: Hydrogens have been added in their riding positions
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Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 20.931 Å2
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Refinement step | Cycle: LAST / Resolution: 2.4→49.794 Å
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LS refinement shell |
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