+Open data
-Basic information
Entry | Database: PDB / ID: 6krq | |||||||||
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Title | Peroxiredoxin from Aeropyrum pernix K1 (ApPrx) 0Cys F80A mutant | |||||||||
Components | Peroxiredoxin | |||||||||
Keywords | OXIDOREDUCTASE / Peroxiredoxin | |||||||||
Function / homology | Function and homology information thioredoxin-dependent peroxiredoxin activity / thioredoxin-dependent peroxiredoxin / peroxiredoxin activity / antioxidant activity / cellular response to hydrogen peroxide / identical protein binding / cytoplasm Similarity search - Function | |||||||||
Biological species | Aeropyrum pernix (archaea) | |||||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 2.1 Å | |||||||||
Authors | Himiyama, T. / Nakamura, T. | |||||||||
Funding support | Japan, 2items
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Citation | Journal: Protein Sci. / Year: 2020 Title: Disassembly of the ring-type decameric structure of peroxiredoxin from Aeropyrum pernix K1 by amino acid mutation. Authors: Himiyama, T. / Nakamura, T. | |||||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 6krq.cif.gz | 513.4 KB | Display | PDBx/mmCIF format |
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PDB format | pdb6krq.ent.gz | 410.8 KB | Display | PDB format |
PDBx/mmJSON format | 6krq.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/kr/6krq ftp://data.pdbj.org/pub/pdb/validation_reports/kr/6krq | HTTPS FTP |
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-Related structure data
Related structure data | 6krkC 6krmC 6krpC 6krrC 6krsC 2e2gS C: citing same article (ref.) S: Starting model for refinement |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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-Components
#1: Protein | Mass: 27840.793 Da / Num. of mol.: 10 / Mutation: C50S,F80A,C207S,C213S Source method: isolated from a genetically manipulated source Source: (gene. exp.) Aeropyrum pernix (strain ATCC 700893 / DSM 11879 / JCM 9820 / NBRC 100138 / K1) (archaea) Strain: ATCC 700893 / DSM 11879 / JCM 9820 / NBRC 100138 / K1 Gene: APE_2278 / Production host: Escherichia coli (E. coli) / References: UniProt: Q9Y9L0, peroxiredoxin #2: Chemical | ChemComp-CIT / #3: Water | ChemComp-HOH / | Has ligand of interest | N | |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 2.66 Å3/Da / Density % sol: 53.84 % |
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Crystal grow | Temperature: 293 K / Method: vapor diffusion, hanging drop / pH: 5.6 Details: 0.1 M Sodium citrate tribasic dihydrate pH 5.6, 1.0 M Ammonium phosphate monobasic |
-Data collection
Diffraction | Mean temperature: 100 K / Serial crystal experiment: N |
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Diffraction source | Source: SYNCHROTRON / Site: SPring-8 / Beamline: BL44XU / Wavelength: 0.9 Å |
Detector | Type: DECTRIS EIGER X 16M / Detector: PIXEL / Date: Jul 14, 2019 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.9 Å / Relative weight: 1 |
Reflection | Resolution: 2.1→50 Å / Num. obs: 168524 / % possible obs: 97.8 % / Redundancy: 3.5 % / Rmerge(I) obs: 0.102 / Net I/σ(I): 7.5 |
Reflection shell | Resolution: 2.1→2.14 Å / Rmerge(I) obs: 0.519 / Mean I/σ(I) obs: 2.4 / Num. unique obs: 8311 |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: 2E2G Resolution: 2.1→49.231 Å / Cor.coef. Fo:Fc: 0.961 / Cor.coef. Fo:Fc free: 0.934 / SU B: 4.992 / SU ML: 0.128 / Cross valid method: THROUGHOUT / ESU R: 0.188 / ESU R Free: 0.171
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Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 31.818 Å2
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Refinement step | Cycle: LAST / Resolution: 2.1→49.231 Å
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Refine LS restraints |
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LS refinement shell |
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