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Open data
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Basic information
Entry | Database: PDB / ID: 6kmz | ||||||
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Title | caspase-4 P22/P10 C258A in complex with human GSDMD-C domain | ||||||
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![]() | IMMUNE SYSTEM / pyroptosis | ||||||
Function / homology | ![]() caspase-4 / non-canonical inflammasome complex / positive regulation of interleukin-18-mediated signaling pathway / non-canonical inflammasome complex assembly / pore complex assembly / AIM2 inflammasome complex / IPAF inflammasome complex / NLRP1 inflammasome complex / : / Release of apoptotic factors from the mitochondria ...caspase-4 / non-canonical inflammasome complex / positive regulation of interleukin-18-mediated signaling pathway / non-canonical inflammasome complex assembly / pore complex assembly / AIM2 inflammasome complex / IPAF inflammasome complex / NLRP1 inflammasome complex / : / Release of apoptotic factors from the mitochondria / wide pore channel activity / NLRP3 inflammasome complex / CARD domain binding / caspase binding / cardiolipin binding / Regulation of TLR by endogenous ligand / phosphatidic acid binding / positive regulation of tumor necrosis factor-mediated signaling pathway / Interleukin-1 processing / phosphatidylinositol-4-phosphate binding / phosphatidylserine binding / pyroptotic inflammatory response / protein autoprocessing / protein maturation / protein secretion / intrinsic apoptotic signaling pathway in response to endoplasmic reticulum stress / Pyroptosis / Purinergic signaling in leishmaniasis infection / phosphatidylinositol-4,5-bisphosphate binding / intrinsic apoptotic signaling pathway / positive regulation of interleukin-1 beta production / mitochondrial membrane / lipopolysaccharide binding / NOD1/2 Signaling Pathway / protein homooligomerization / positive regulation of inflammatory response / specific granule lumen / cellular response to amyloid-beta / positive regulation of neuron apoptotic process / tertiary granule lumen / regulation of inflammatory response / defense response to Gram-negative bacterium / ficolin-1-rich granule lumen / defense response to Gram-positive bacterium / defense response to bacterium / inflammatory response / cysteine-type endopeptidase activity / innate immune response / lipid binding / apoptotic process / Neutrophil degranulation / endoplasmic reticulum membrane / endoplasmic reticulum / protein-containing complex / mitochondrion / proteolysis / extracellular space / extracellular region / nucleoplasm / membrane / plasma membrane / cytoplasm / cytosol Similarity search - Function | ||||||
Biological species | ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Ding, J. / Sun, Q. | ||||||
![]() | ![]() Title: Structural Mechanism for GSDMD Targeting by Autoprocessed Caspases in Pyroptosis. Authors: Wang, K. / Sun, Q. / Zhong, X. / Zeng, M. / Zeng, H. / Shi, X. / Li, Z. / Wang, Y. / Zhao, Q. / Shao, F. / Ding, J. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 268.4 KB | Display | ![]() |
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PDB format | ![]() | 216.6 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 494.8 KB | Display | ![]() |
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Full document | ![]() | 518.4 KB | Display | |
Data in XML | ![]() | 46.3 KB | Display | |
Data in CIF | ![]() | 62.8 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 6kmtC ![]() 6kmuSC ![]() 6kmvC ![]() 6kn0C ![]() 6kn1C C: citing same article ( S: Starting model for refinement |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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2 | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 20987.738 Da / Num. of mol.: 4 / Mutation: C258A Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() #2: Protein | | Mass: 20757.803 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() #3: Protein | | Mass: 20872.891 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() #4: Protein | Mass: 10423.982 Da / Num. of mol.: 4 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 5.32 Å3/Da / Density % sol: 76.89 % |
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Crystal grow | Temperature: 293 K / Method: vapor diffusion, sitting drop Details: 100 mM Tris-HCl pH 7.5, 200 mM sodium tartrate, 20% (w/v) polyethylene glycol 3350, 5% glycerol and 4% 1,1,1,3,3,3-hexafluoro-2-propanol |
-Data collection
Diffraction | Mean temperature: 93 K / Serial crystal experiment: N |
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Diffraction source | Source: ![]() ![]() |
Detector | Type: DECTRIS PILATUS3 6M / Detector: PIXEL / Date: Dec 8, 2018 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.97891 Å / Relative weight: 1 |
Reflection | Resolution: 3.61→49.14 Å / Num. obs: 38808 / % possible obs: 99.5 % / Redundancy: 6.43 % / CC1/2: 0.999 / Rmerge(I) obs: 0.094 / Net I/σ(I): 13.83 |
Reflection shell | Resolution: 3.61→3.7 Å / Rmerge(I) obs: 1.106 / Mean I/σ(I) obs: 1.8 / Num. unique obs: 2781 / CC1/2: 0.768 / % possible all: 97.7 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: 6KMU Resolution: 3.61→45.48 Å / Cross valid method: FREE R-VALUE
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Refinement step | Cycle: LAST / Resolution: 3.61→45.48 Å
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LS refinement shell | Resolution: 3.61→3.73 Å
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