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Open data
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Basic information
Entry | Database: PDB / ID: 6kjg | ||||||
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Title | Crystal structure of PsoF | ||||||
![]() | Dual-functional monooxygenase/methyltransferase psoF | ||||||
![]() | OXIDOREDUCTASE / metyltransferase | ||||||
Function / homology | ![]() fumagillin biosynthetic process / N,N-dimethylaniline monooxygenase activity / Oxidoreductases / monooxygenase activity / Transferases; Transferring one-carbon groups; Methyltransferases / methyltransferase activity / NADP binding / flavin adenine dinucleotide binding / methylation Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Hara, K. / Hashimoto, H. / Matsushita, T. / Tsunematsu, Y. / Watanabe, K. | ||||||
![]() | ![]() Title: Functional and Structural Analyses oftrans C-Methyltransferase in Fungal Polyketide Biosynthesis. Authors: Kishimoto, S. / Tsunematsu, Y. / Matsushita, T. / Hara, K. / Hashimoto, H. / Tang, Y. / Watanabe, K. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 221.2 KB | Display | ![]() |
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PDB format | ![]() | 175.6 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 459.1 KB | Display | ![]() |
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Full document | ![]() | 472.7 KB | Display | |
Data in XML | ![]() | 42.3 KB | Display | |
Data in CIF | ![]() | 60.5 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 6kjiC ![]() 5thyS S: Starting model for refinement C: citing same article ( |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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1 | ![]()
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2 | ![]()
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3 | ![]()
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Unit cell |
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Components on special symmetry positions |
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Components
#1: Protein | Mass: 41732.039 Da / Num. of mol.: 3 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() Strain: ATCC MYA-4609 / Af293 / CBS 101355 / FGSC A1100 / Gene: psoF, AFUA_8G00440 / Production host: ![]() ![]() References: UniProt: Q4WAZ0, Oxidoreductases, Transferases; Transferring one-carbon groups; Methyltransferases #2: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.57 Å3/Da / Density % sol: 52.1 % |
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Crystal grow | Temperature: 293 K / Method: vapor diffusion, hanging drop / Details: PEG 3350, sodium fluoride, bis-tris propane pH 6.5 |
-Data collection
Diffraction | Mean temperature: 100 K / Serial crystal experiment: N |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: DECTRIS PILATUS3 S 6M / Detector: PIXEL / Date: Nov 11, 2017 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.98 Å / Relative weight: 1 |
Reflection | Resolution: 1.99→20 Å / Num. obs: 80787 / % possible obs: 98.3 % / Redundancy: 3 % / Rmerge(I) obs: 0.054 / Net I/σ(I): 11.87 |
Reflection shell | Resolution: 1.99→2.09 Å / Rmerge(I) obs: 0.332 / Mean I/σ(I) obs: 2.48 / Num. unique obs: 12540 / % possible all: 95.8 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: 5THY Resolution: 1.99→19.8 Å / Cross valid method: FREE R-VALUE
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Refinement step | Cycle: LAST / Resolution: 1.99→19.8 Å
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