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- PDB-6jx2: Crystal structure of Ketol-acid reductoisomerase from Corynebacte... -
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Open data
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Basic information
Entry | Database: PDB / ID: 6jx2 | ||||||
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Title | Crystal structure of Ketol-acid reductoisomerase from Corynebacterium glutamicum | ||||||
![]() | Ketol-acid reductoisomerase (NADP(+)) | ||||||
![]() | OXIDOREDUCTASE / Ketol-acid reductoisomerase | ||||||
Function / homology | ![]() 2-dehydropantoate 2-reductase activity / ketol-acid reductoisomerase (NADP+) / ketol-acid reductoisomerase activity / valine biosynthetic process / isoleucine biosynthetic process / NADP binding / magnesium ion binding Similarity search - Function | ||||||
Biological species | ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Lee, D. / Hong, J. / Kim, K.-J. | ||||||
![]() | ![]() Title: Crystal Structure and Biochemical Characterization of Ketol-Acid Reductoisomerase fromCorynebacterium glutamicum. Authors: Lee, D. / Hong, J. / Kim, K.J. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 255.1 KB | Display | ![]() |
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PDB format | ![]() | 205 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 1.3 MB | Display | ![]() |
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Full document | ![]() | 1.3 MB | Display | |
Data in XML | ![]() | 46.2 KB | Display | |
Data in CIF | ![]() | 63.5 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 4ypoS S: Starting model for refinement |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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1 | ![]()
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2 | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 37021.539 Da / Num. of mol.: 4 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() Strain: ATCC 13032 / Gene: ilvC, Cgl1273, cg1437 / Plasmid: pET30a / Production host: ![]() ![]() References: UniProt: Q57179, ketol-acid reductoisomerase (NADP+) #2: Chemical | ChemComp-MG / #3: Chemical | #4: Chemical | ChemComp-EDO / #5: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.18 Å3/Da / Density % sol: 43.54 % |
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Crystal grow | Temperature: 293 K / Method: vapor diffusion, hanging drop / pH: 5.5 Details: 25 % (w/v) polyethylene glycol (PEG) 3350, 0.1 M Bis-Tris, pH 5.5, 0.2 M Magnesium Chloride |
-Data collection
Diffraction | Mean temperature: 100 K / Serial crystal experiment: N |
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Diffraction source | Source: ![]() ![]() |
Detector | Type: ADSC QUANTUM 270 / Detector: CCD / Date: Nov 6, 2016 |
Radiation | Monochromator: Double Crystal Monochromator / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.97934 Å / Relative weight: 1 |
Reflection | Resolution: 2.6→78.91 Å / Num. obs: 37218 / % possible obs: 98.1 % / Redundancy: 4.2 % / Rmerge(I) obs: 0.091 / Net I/σ(I): 20.6 |
Reflection shell | Resolution: 2.6→2.64 Å / Num. unique obs: 37218 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: 4YPO Resolution: 2.6→78.91 Å / Cor.coef. Fo:Fc: 0.951 / Cor.coef. Fo:Fc free: 0.899 / SU B: 14.245 / SU ML: 0.297 / Cross valid method: THROUGHOUT / σ(F): 0 / ESU R Free: 0.378 Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS U VALUES : REFINED INDIVIDUALLY
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Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso max: 140.24 Å2 / Biso mean: 54.779 Å2 / Biso min: 24.02 Å2
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Refinement step | Cycle: final / Resolution: 2.6→78.91 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 2.601→2.669 Å / Rfactor Rfree error: 0 / Total num. of bins used: 20
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