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- PDB-6jaq: Crystal structure of ABC transporter alpha-glycoside-binding muta... -
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Open data
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Basic information
Entry | Database: PDB / ID: 6jaq | ||||||
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Title | Crystal structure of ABC transporter alpha-glycoside-binding mutant protein R356A in complex with glucose | ||||||
![]() | ABC transporter, periplasmic substrate-binding protein![]() | ||||||
![]() | SUGAR BINDING PROTEIN / Carbohydrate-bindingsite / alpha-glycoside-binding protein / Ligand selection / Multi-substrate transporter / ![]() | ||||||
Function / homology | ![]() Bacterial extracellular solute-binding protein / Bacterial extracellular solute-binding protein / Bacterial extracellular solute-binding protein / Periplasmic binding protein-like II / D-Maltodextrin-Binding Protein; domain 2 / 3-Layer(aba) Sandwich / Alpha Beta Similarity search - Domain/homology | ||||||
Biological species | ![]() ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Kanaujia, S.P. / Chandravanshi, M. / Gogoi, P. | ||||||
Funding support | ![]()
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![]() | ![]() Title: Structural and thermodynamic correlation illuminates the selective transport mechanism of disaccharide alpha-glycosides through ABC transporter. Authors: Chandravanshi, M. / Gogoi, P. / Kanaujia, S.P. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 110.9 KB | Display | ![]() |
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PDB format | ![]() | 81.6 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
Related structure data | ![]() 6j9wSC ![]() 6j9yC ![]() 6jadC ![]() 6jagC ![]() 6jahC ![]() 6jaiC ![]() 6jalC ![]() 6jamC ![]() 6janC ![]() 6jaoC ![]() 6japC ![]() 6jarC ![]() 6jazC ![]() 6jb0C ![]() 6jb4C ![]() 6jbaC ![]() 6jbbC ![]() 6jbeC S: Starting model for refinement C: citing same article ( |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components on special symmetry positions |
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Components
-Protein / Sugars , 2 types, 2 molecules A![](data/chem/img/GLC.gif)
![](data/chem/img/GLC.gif)
#1: Protein | ![]() Mass: 46081.883 Da / Num. of mol.: 1 / Mutation: R356A Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() Strain: HB8 / ATCC 27634 / DSM 579 / Gene: TTHA0356 / Plasmid: pET28a / Production host: ![]() ![]() ![]() |
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#2: Sugar | ChemComp-GLC / ![]() |
-Non-polymers , 4 types, 536 molecules ![](data/chem/img/CIT.gif)
![](data/chem/img/EDO.gif)
![](data/chem/img/GOL.gif)
![](data/chem/img/HOH.gif)
![](data/chem/img/EDO.gif)
![](data/chem/img/GOL.gif)
![](data/chem/img/HOH.gif)
#3: Chemical | ChemComp-CIT / ![]() | ||||
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#4: Chemical | ![]() #5: Chemical | ![]() #6: Water | ChemComp-HOH / | ![]() |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.88 Å3/Da / Density % sol: 57.29 % / Description: Tetragonal |
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Crystal grow![]() | Temperature: 277 K / Method: microbatch / pH: 6.4 Details: 0.04 M Citric Acid, 0.06 M Bis-Tris Propane, 16% PEG 3350 |
-Data collection
Diffraction | Mean temperature: 100 K / Serial crystal experiment: N |
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Diffraction source | Source: ![]() |
Detector | Type: RIGAKU RAXIS IV++ / Detector: IMAGE PLATE / Date: Mar 5, 2018 / Details: VariMax HF |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength![]() |
Reflection | Resolution: 1.95→73.24 Å / Num. obs: 39565 / % possible obs: 100 % / Redundancy: 14.7 % / CC1/2: 0.999 / Rmerge(I) obs: 0.108 / Rpim(I) all: 0.041 / Rrim(I) all: 0.116 / Net I/σ(I): 22.2 |
Reflection shell | Resolution: 1.95→8.94 Å / Redundancy: 14.7 % / Rmerge(I) obs: 0.483 / Mean I/σ(I) obs: 6 / Num. unique obs: 2725 / CC1/2: 0.952 / Rpim(I) all: 0.185 / Rrim(I) all: 0.517 / % possible all: 100 |
-Phasing
Phasing![]() | Method: ![]() | |||||||||
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Phasing MR | Model details: Phaser MODE: MR_AUTO
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Processing
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Refinement | Method to determine structure![]() ![]() Starting model: 6J9W Resolution: 1.95→73.24 Å / Cor.coef. Fo:Fc: 0.968 / Cor.coef. Fo:Fc free: 0.952 / SU B: 2.257 / SU ML: 0.064 / Cross valid method: THROUGHOUT / σ(F): 0 / ESU R: 0.108 / ESU R Free: 0.106 Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS U VALUES : REFINED INDIVIDUALLY
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Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso max: 79.93 Å2 / Biso mean: 16.572 Å2 / Biso min: 7.48 Å2
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Refinement step | Cycle: final / Resolution: 1.95→73.24 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 1.95→2.001 Å / Rfactor Rfree error: 0 / Total num. of bins used: 20
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