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- PDB-6jag: Crystal structure of ABC transporter alpha-glycoside-binding prot... -
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Open data
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Basic information
Entry | Database: PDB / ID: 6jag | |||||||||
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Title | Crystal structure of ABC transporter alpha-glycoside-binding protein in complex with sucrose | |||||||||
![]() | ABC transporter, periplasmic substrate-binding protein | |||||||||
![]() | SUGAR BINDING PROTEIN / Carbohydrate-bindingsite / alpha-glycoside-binding protein / Ligand selection / Multi-substrate transporter / Sugar replacement / Venus Fly-trap mechanism | |||||||||
Function / homology | ![]() : / Bacterial extracellular solute-binding protein / Bacterial extracellular solute-binding protein / Bacterial extracellular solute-binding protein / Periplasmic binding protein-like II / D-Maltodextrin-Binding Protein; domain 2 / 3-Layer(aba) Sandwich / Alpha Beta Similarity search - Domain/homology | |||||||||
Biological species | ![]() ![]() | |||||||||
Method | ![]() ![]() ![]() | |||||||||
![]() | Kanaujia, S.P. / Chandravanshi, M. / Gogoi, P. | |||||||||
Funding support | ![]()
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![]() | ![]() Title: Structural and thermodynamic correlation illuminates the selective transport mechanism of disaccharide alpha-glycosides through ABC transporter. Authors: Chandravanshi, M. / Gogoi, P. / Kanaujia, S.P. | |||||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 113.6 KB | Display | ![]() |
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PDB format | ![]() | 82.4 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
Related structure data | ![]() 6j9wSC ![]() 6j9yC ![]() 6jadC ![]() 6jahC ![]() 6jaiC ![]() 6jalC ![]() 6jamC ![]() 6janC ![]() 6jaoC ![]() 6japC ![]() 6jaqC ![]() 6jarC ![]() 6jazC ![]() 6jb0C ![]() 6jb4C ![]() 6jbaC ![]() 6jbbC ![]() 6jbeC S: Starting model for refinement C: citing same article ( |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components on special symmetry positions |
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Components
#1: Protein | Mass: 46168.000 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() Strain: HB8 / ATCC 27634 / DSM 579 / Gene: TTHA0356 / Plasmid: pET28a / Production host: ![]() ![]() |
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#2: Polysaccharide | beta-D-fructofuranose-(2-1)-alpha-D-glucopyranose / sucrose |
#3: Chemical | ChemComp-CIT / |
#4: Chemical | ChemComp-GOL / |
#5: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.88 Å3/Da / Density % sol: 57.31 % / Description: morphology: Tetragonal |
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Crystal grow | Temperature: 277 K / Method: microbatch / pH: 6.4 Details: 0.04 M Citric Acid, 0.06 M Bis-Tris Propane, 16% PEG 3350 |
-Data collection
Diffraction | Mean temperature: 100 K / Serial crystal experiment: N |
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Diffraction source | Source: ![]() |
Detector | Type: RIGAKU RAXIS IV++ / Detector: IMAGE PLATE / Date: Mar 1, 2018 / Details: VariMax HF |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.5418 Å / Relative weight: 1 |
Reflection | Resolution: 1.85→73.41 Å / Num. obs: 46411 / % possible obs: 100 % / Redundancy: 17.7 % / CC1/2: 0.999 / Rmerge(I) obs: 0.107 / Rpim(I) all: 0.036 / Rrim(I) all: 0.113 / Net I/σ(I): 24.3 |
Reflection shell | Resolution: 1.85→9.06 Å / Redundancy: 16.7 % / Rmerge(I) obs: 0.573 / Mean I/σ(I) obs: 5.8 / Num. unique obs: 2826 / CC1/2: 0.944 / Rpim(I) all: 0.203 / Rrim(I) all: 0.608 / % possible all: 100 |
-Phasing
Phasing | Method: ![]() | |||||||||
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Phasing MR | Model details: Phaser MODE: MR_AUTO
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Processing
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Refinement | Method to determine structure: ![]() Starting model: 6J9W Resolution: 1.85→73.41 Å / Cor.coef. Fo:Fc: 0.97 / Cor.coef. Fo:Fc free: 0.949 / SU B: 1.734 / SU ML: 0.053 / Cross valid method: THROUGHOUT / σ(F): 0 / ESU R: 0.09 / ESU R Free: 0.093 Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS U VALUES : REFINED INDIVIDUALLY
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Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso max: 75.37 Å2 / Biso mean: 15.964 Å2 / Biso min: 7.14 Å2
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Refinement step | Cycle: final / Resolution: 1.85→73.41 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 1.85→1.898 Å / Rfactor Rfree error: 0 / Total num. of bins used: 20
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