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- PDB-6jae: Crystal structure of Trypanosoma brucei gambiense glycerol kinase... -
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Open data
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Basic information
Entry | Database: PDB / ID: 6jae | ||||||
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Title | Crystal structure of Trypanosoma brucei gambiense glycerol kinase complex with Pi (pyrophosphatase reaction) | ||||||
![]() | Glycerol kinase | ||||||
![]() | TRANSFERASE / TRYPANOSOMA / GLYCEROL KINASE / SUGAR KINASE SUPERFAMILY / GLYCOSOME | ||||||
Function / homology | ![]() glycerol kinase / glycerol-3-phosphate biosynthetic process / glycerol kinase activity / glycerol catabolic process / triglyceride metabolic process / phosphorylation / mitochondrion / ATP binding / cytosol Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Balogun, E.O. / Chishima, T. / Ichinose, M. / Inaoka, D.K. / Kido, Y. / Ibrahim, B. / Bringaud, F. / de Koning, H. / McKerrow, J.H. / Watanabe, Y. ...Balogun, E.O. / Chishima, T. / Ichinose, M. / Inaoka, D.K. / Kido, Y. / Ibrahim, B. / Bringaud, F. / de Koning, H. / McKerrow, J.H. / Watanabe, Y. / Nozaki, T. / Michels, P.A.M. / Harada, S. / Kita, K. / Shiba, T. | ||||||
![]() | ![]() Title: Glycerol Kinase of African Human Trypanosomes Possesses a Pyrophosphatase Activity. Authors: Balogun, E.O. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 393.3 KB | Display | ![]() |
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PDB format | ![]() | 322.2 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 489.1 KB | Display | ![]() |
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Full document | ![]() | 519 KB | Display | |
Data in XML | ![]() | 69.4 KB | Display | |
Data in CIF | ![]() | 94.1 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 6j9xC ![]() 6jafC ![]() 3wxlS S: Starting model for refinement C: citing same article ( |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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2 | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 57064.625 Da / Num. of mol.: 4 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() Gene: gk / Plasmid: PET151/D-TOPO / Production host: ![]() ![]() #2: Chemical | #3: Chemical | ChemComp-GOL / #4: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.6 Å3/Da / Density % sol: 52.73 % |
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Crystal grow | Temperature: 293 K / Method: vapor diffusion, hanging drop / pH: 7.5 / Details: 8-18% PEG 400, 0.1M HEPES, 11% 1,6-HEXANEDIOL |
-Data collection
Diffraction | Mean temperature: 100 K / Serial crystal experiment: N |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: DECTRIS EIGER X 16M / Detector: PIXEL / Date: Jun 8, 2018 |
Radiation | Monochromator: SI(111) / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.9 Å / Relative weight: 1 |
Reflection | Resolution: 2.3→50 Å / Num. obs: 103029 / % possible obs: 95.8 % / Redundancy: 1.8 % / Rmerge(I) obs: 0.02 / Net I/σ(I): 19.4 |
Reflection shell | Resolution: 2.3→2.44 Å / Rmerge(I) obs: 0.223 / Mean I/σ(I) obs: 2.8 / Num. unique obs: 31789 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: 3WXL Resolution: 2.3→19.84 Å / Cor.coef. Fo:Fc: 0.956 / Cor.coef. Fo:Fc free: 0.929 / SU B: 6.978 / SU ML: 0.174 / Cross valid method: THROUGHOUT / ESU R: 0.336 / ESU R Free: 0.251 / Stereochemistry target values: MAXIMUM LIKELIHOOD / Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS
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Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å / Solvent model: MASK | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 57.144 Å2
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Refinement step | Cycle: 1 / Resolution: 2.3→19.84 Å
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Refine LS restraints |
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