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Open data
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Basic information
Entry | Database: PDB / ID: 6j68 | ||||||
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Title | Structure of KIBRA and LATS1 Complex | ||||||
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![]() | CELL CYCLE / Tandem WW domain / Tandem PY motif / HIPPO Signaling | ||||||
Function / homology | ![]() inner cell mass cell fate commitment / inner cell mass cellular morphogenesis / regulation of hippo signaling / Signaling by Hippo / regulation of ubiquitin-dependent protein catabolic process / sister chromatid segregation / regulation of transforming growth factor beta receptor signaling pathway / cytoplasmic sequestering of protein / negative regulation of organ growth / hippo signaling ...inner cell mass cell fate commitment / inner cell mass cellular morphogenesis / regulation of hippo signaling / Signaling by Hippo / regulation of ubiquitin-dependent protein catabolic process / sister chromatid segregation / regulation of transforming growth factor beta receptor signaling pathway / cytoplasmic sequestering of protein / negative regulation of organ growth / hippo signaling / regulation of actin filament polymerization / mammary gland epithelial cell differentiation / regulation of intracellular estrogen receptor signaling pathway / negative regulation of cyclin-dependent protein serine/threonine kinase activity / regulation of organ growth / negative regulation of protein localization to nucleus / microtubule organizing center / negative regulation of hippo signaling / regulation of protein-containing complex assembly / keratinocyte differentiation / hormone-mediated signaling pathway / nuclear estrogen receptor binding / negative regulation of canonical Wnt signaling pathway / kinase binding / ruffle membrane / spindle pole / G1/S transition of mitotic cell cycle / G2/M transition of mitotic cell cycle / protein localization / cell migration / positive regulation of peptidyl-serine phosphorylation / protein-macromolecule adaptor activity / midbody / peptidyl-serine phosphorylation / positive regulation of MAPK cascade / molecular adaptor activity / transcription coactivator activity / non-specific serine/threonine protein kinase / positive regulation of apoptotic process / negative regulation of cell population proliferation / cell division / protein phosphorylation / protein serine kinase activity / protein serine/threonine kinase activity / regulation of DNA-templated transcription / protein kinase binding / perinuclear region of cytoplasm / negative regulation of transcription by RNA polymerase II / magnesium ion binding / protein-containing complex / ATP binding / nucleus / cytosol / cytoplasm Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Lin, Z. / Yang, Z. / Ji, Z. / Zhang, M. | ||||||
![]() | ![]() Title: Decoding WW domain tandem-mediated target recognitions in tissue growth and cell polarity. Authors: Lin, Z. / Yang, Z. / Xie, R. / Ji, Z. / Guan, K. / Zhang, M. | ||||||
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 123.1 KB | Display | ![]() |
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PDB format | ![]() | 95.4 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
Related structure data | ![]() 6jjwC ![]() 6jjxC ![]() 6jjyC ![]() 6jjzC ![]() 6jk0C ![]() 6jk1C C: citing same article ( |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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1 | ![]()
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2 | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 16036.611 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() #2: Protein/peptide | Mass: 2926.220 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() References: UniProt: Q8BYR2, non-specific serine/threonine protein kinase #3: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 3.07 Å3/Da / Density % sol: 59.94 % |
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Crystal grow | Temperature: 289 K / Method: vapor diffusion / pH: 8 / Details: 0.1M Tris, 28% PEG 4000 |
-Data collection
Diffraction | Mean temperature: 100 K / Serial crystal experiment: N |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: ADSC QUANTUM 315r / Detector: CCD / Date: Jul 13, 2015 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.97854 Å / Relative weight: 1 |
Reflection | Resolution: 2.495→50 Å / Num. obs: 15953 / % possible obs: 99.5 % / Redundancy: 11.2 % / Rmerge(I) obs: 0.102 / Net I/σ(I): 23.6 |
Reflection shell | Resolution: 2.495→2.54 Å / Rmerge(I) obs: 0.982 / Num. unique obs: 713 |
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Processing
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Refinement | Method to determine structure: ![]()
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL | ||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2.495→49.035 Å
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Refine LS restraints |
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LS refinement shell |
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Refinement TLS params. | Method: refined / Origin x: 13.3476 Å / Origin y: -5.0154 Å / Origin z: -20.1226 Å
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Refinement TLS group | Selection details: all |