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- PDB-6j2u: Crystal structure of Tyrosinase caddy protein(MelC1)with Tyrosina... -
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Open data
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Basic information
Entry | Database: PDB / ID: 6j2u | ||||||
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Title | Crystal structure of Tyrosinase caddy protein(MelC1)with Tyrosinase (MelC2)from Streptomyces avermitilis in complex with Zinc ion | ||||||
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![]() | OXIDOREDUCTASE / Citrate synthase / Metallosphaera sedula | ||||||
Function / homology | ![]() melanin biosynthetic process / oxidoreductase activity / copper ion binding Similarity search - Function | ||||||
Biological species | ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Lee, S.-H. / Hong, H. / Kim, K.-J. | ||||||
![]() | ![]() Title: Crystal structure of Tyrosinase caddy protein(MelC1)with tyrosinase (MelC2)from Streptomyces avermitilis in complex with Zinc ion Authors: Lee, S.-H. / Hong, H. / Kim, K.-J. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 96.5 KB | Display | ![]() |
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PDB format | ![]() | 70.2 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 433.8 KB | Display | ![]() |
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Full document | ![]() | 435.6 KB | Display | |
Data in XML | ![]() | 19.6 KB | Display | |
Data in CIF | ![]() | 31 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 5z0dS S: Starting model for refinement |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 12111.513 Da / Num. of mol.: 1 / Fragment: MelC1 domain Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() Production host: ![]() ![]() References: UniProt: Q93HL1 | ||
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#2: Protein | Mass: 32249.723 Da / Num. of mol.: 1 / Fragment: MelC2 domain Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() Production host: ![]() ![]() References: UniProt: Q93HL2 | ||
#3: Chemical | #4: Water | ChemComp-HOH / | |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.23 Å3/Da / Density % sol: 44.9 % |
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Crystal grow | Temperature: 295 K / Method: vapor diffusion, sitting drop Details: 20% polyethylene glycol 3350 and 0.2 M Lithium chloride |
-Data collection
Diffraction | Mean temperature: 100 K / Serial crystal experiment: N |
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Diffraction source | Source: ![]() ![]() |
Detector | Type: ADSC QUANTUM 270 / Detector: CCD / Date: Dec 18, 2018 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.979 Å / Relative weight: 1 |
Reflection | Resolution: 1.3→60.12 Å / Num. obs: 91179 / % possible obs: 98.4 % / Redundancy: 3.5 % / CC1/2: 0.993 / Net I/σ(I): 29.3 |
Reflection shell | Resolution: 1.3→1.32 Å / Num. unique obs: 4459 / CC1/2: 0.9 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: 5Z0D Resolution: 1.3→60.12 Å / Cor.coef. Fo:Fc: 0.972 / Cor.coef. Fo:Fc free: 0.968 / Cross valid method: FREE R-VALUE
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Displacement parameters | Biso max: 59.83 Å2 / Biso min: 2.31 Å2 | ||||||||||||||||||||||||
Refinement step | Cycle: final / Resolution: 1.3→60.12 Å
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LS refinement shell | Resolution: 1.3→1.32 Å
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