+Open data
-Basic information
Entry | Database: PDB / ID: 6ixh | ||||||
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Title | Type VI secretion system membrane core complex | ||||||
Components |
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Keywords | MEMBRANE PROTEIN / Type VI secretion system membrane core complex | ||||||
Function / homology | Function and homology information | ||||||
Biological species | Escherichia coli (E. coli) | ||||||
Method | ELECTRON MICROSCOPY / single particle reconstruction / cryo EM / Resolution: 4 Å | ||||||
Authors | Yin, M. / Yan, Z. | ||||||
Funding support | China, 1items
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Citation | Journal: Cell Res / Year: 2019 Title: Architecture of type VI secretion system membrane core complex. Authors: Meng Yin / Zhaofeng Yan / Xueming Li / | ||||||
History |
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-Structure visualization
Movie |
Movie viewer |
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Structure viewer | Molecule: MolmilJmol/JSmol |
-Downloads & links
-Download
PDBx/mmCIF format | 6ixh.cif.gz | 1.2 MB | Display | PDBx/mmCIF format |
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PDB format | pdb6ixh.ent.gz | 963.2 KB | Display | PDB format |
PDBx/mmJSON format | 6ixh.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 6ixh_validation.pdf.gz | 997.4 KB | Display | wwPDB validaton report |
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Full document | 6ixh_full_validation.pdf.gz | 1 MB | Display | |
Data in XML | 6ixh_validation.xml.gz | 157.1 KB | Display | |
Data in CIF | 6ixh_validation.cif.gz | 246.3 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/ix/6ixh ftp://data.pdbj.org/pub/pdb/validation_reports/ix/6ixh | HTTPS FTP |
-Related structure data
Related structure data | 9747MC M: map data used to model this data C: citing same article (ref.) |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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1 |
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-Components
#1: Protein | Mass: 19271.090 Da / Num. of mol.: 15 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Escherichia coli (strain 55989 / EAEC) (bacteria) Strain: 55989 / EAEC / Gene: EC55989_3320 / Production host: Escherichia coli BL21(DE3) (bacteria) / Strain (production host): BL21(DE3) / References: UniProt: B7LFS8 #2: Protein | Mass: 127113.070 Da / Num. of mol.: 10 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Escherichia coli (strain 55989 / EAEC) (bacteria) Strain: 55989 / EAEC / Gene: EC55989_3325 / Production host: Escherichia coli BL21(DE3) (bacteria) / Strain (production host): BL21(DE3) / References: UniProt: B7LFU0 |
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-Experimental details
-Experiment
Experiment | Method: ELECTRON MICROSCOPY |
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EM experiment | Aggregation state: PARTICLE / 3D reconstruction method: single particle reconstruction |
-Sample preparation
Component | Name: Type VI Secretion System membrane core complex TssJLM / Type: COMPLEX / Entity ID: all / Source: RECOMBINANT |
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Source (natural) | Organism: Escherichia coli 55989 (bacteria) / Strain: 55989 |
Source (recombinant) | Organism: Escherichia coli BL21(DE3) (bacteria) / Strain: BL21(DE3) |
Buffer solution | pH: 8 |
Specimen | Embedding applied: NO / Shadowing applied: NO / Staining applied: NO / Vitrification applied: YES |
Vitrification | Cryogen name: ETHANE |
-Electron microscopy imaging
Experimental equipment | Model: Titan Krios / Image courtesy: FEI Company |
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Microscopy | Model: FEI TITAN KRIOS |
Electron gun | Electron source: FIELD EMISSION GUN / Accelerating voltage: 300 kV / Illumination mode: OTHER |
Electron lens | Mode: BRIGHT FIELD |
Image recording | Electron dose: 50 e/Å2 / Film or detector model: GATAN K2 SUMMIT (4k x 4k) |
-Processing
CTF correction | Type: NONE |
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3D reconstruction | Resolution: 4 Å / Resolution method: FSC 0.143 CUT-OFF / Num. of particles: 44069 / Symmetry type: POINT |