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- PDB-6ipa: C-terminal EMAP II-like domain of p43 refined against twinned data -
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Open data
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Basic information
Entry | Database: PDB / ID: 6ipa | ||||||
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Title | C-terminal EMAP II-like domain of p43 refined against twinned data | ||||||
![]() | aminoacyl-tRNA synthetase-interacting multifunctional protein p43 | ||||||
![]() | RNA BINDING PROTEIN / AIMP1 / EMAPII-LIKE DOMAIN / PLASMODIUM | ||||||
Function / homology | ![]() | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Manickam, Y. / Harlos, K. / Gupta, S. / Sharma, A. | ||||||
Funding support | ![]()
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![]() | ![]() Title: Crystal structures of the two domains that constitute the Plasmodium vivax p43 protein. Authors: Gupta, S. / Chhibber-Goel, J. / Sharma, M. / Parvez, S. / Harlos, K. / Sharma, A. / Yogavel, M. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 271.8 KB | Display | ![]() |
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PDB format | ![]() | 220.3 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 451 KB | Display | ![]() |
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Full document | ![]() | 456.6 KB | Display | |
Data in XML | ![]() | 26.3 KB | Display | |
Data in CIF | ![]() | 36.4 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 5zkeC ![]() 5zkfC ![]() 5zkgSC ![]() 6jreC S: Starting model for refinement C: citing same article ( |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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1 | ![]()
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2 | ![]()
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3 | ![]()
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4 | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 22872.205 Da / Num. of mol.: 4 / Fragment: EMAP II -Like Domain Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() Production host: ![]() ![]() #2: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.19 Å3/Da / Density % sol: 43.89 % |
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Crystal grow | Temperature: 293 K / Method: vapor diffusion, hanging drop Details: 12.5% w/v PEG 1000, 12.5% w/v PEG 3350, 12.5% v/v MPD, 0.02M amino acids, 0.1M MES |
-Data collection
Diffraction | Mean temperature: 100 K / Serial crystal experiment: N | |||||||||||||||
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Diffraction source | Source: ![]() ![]() ![]() | |||||||||||||||
Detector | Type: DECTRIS PILATUS3 6M / Detector: PIXEL / Date: May 5, 2017 | |||||||||||||||
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray | |||||||||||||||
Radiation wavelength | Wavelength: 0.9763 Å / Relative weight: 1 | |||||||||||||||
Reflection twin |
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Reflection | Resolution: 2.47→40.75 Å / Num. obs: 29903 / % possible obs: 100 % / Redundancy: 12.8 % / CC1/2: 1 / Rrim(I) all: 0.181 / Net I/σ(I): 12 | |||||||||||||||
Reflection shell | Resolution: 2.47→2.51 Å / Redundancy: 11.3 % / Mean I/σ(I) obs: 1.3 / Num. unique obs: 1455 / CC1/2: 0.5 / Rrim(I) all: 1.892 / % possible all: 100 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: 5ZKG Resolution: 2.47→36.73 Å / Cor.coef. Fo:Fc: 0.948 / Cor.coef. Fo:Fc free: 0.911 / WRfactor Rfree: 0.2304 / WRfactor Rwork: 0.1788 / FOM work R set: 0.7389 / SU B: 17.217 / SU ML: 0.187 / SU R Cruickshank DPI: 0.0982 / SU Rfree: 0.061 / Cross valid method: THROUGHOUT / σ(F): 0 / ESU R: 0.098 / ESU R Free: 0.061 / Stereochemistry target values: MAXIMUM LIKELIHOOD Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS U VALUES : WITH TLS ADDED
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Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å / Solvent model: MASK | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso max: 106.7 Å2 / Biso mean: 50.161 Å2 / Biso min: 21.38 Å2
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Refinement step | Cycle: final / Resolution: 2.47→36.73 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 2.47→2.533 Å / Rfactor Rfree error: 0 / Total num. of bins used: 20
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Refinement TLS params. | Method: refined / Refine-ID: X-RAY DIFFRACTION
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Refinement TLS group |
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