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- PDB-6iog: Crystal structure of Homoserine O-acetyltransferase from Mycobact... -
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Open data
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Basic information
Entry | Database: PDB / ID: 6iog | ||||||
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Title | Crystal structure of Homoserine O-acetyltransferase from Mycobacterium smegmatis ATCC 19420 | ||||||
![]() | Homoserine O-acetyltransferase | ||||||
![]() | TRANSFERASE / BIOSYNTHETIC PROTEIN | ||||||
Function / homology | ![]() homoserine metabolic process / homoserine O-acetyltransferase / homoserine O-acetyltransferase activity / methionine biosynthetic process / cytoplasm Similarity search - Function | ||||||
Biological species | ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Sagong, H.-Y. / Kim, K.-J. | ||||||
![]() | ![]() Title: Crystal structure and biochemical characterization of O-acetylhomoserine acetyltransferase from Mycobacterium smegmatis ATCC 19420. Authors: Sagong, H.Y. / Hong, J. / Kim, K.J. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 158.6 KB | Display | ![]() |
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PDB format | ![]() | 124.1 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 447.3 KB | Display | ![]() |
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Full document | ![]() | 451.7 KB | Display | |
Data in XML | ![]() | 32 KB | Display | |
Data in CIF | ![]() | 48.5 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 6iohC ![]() 6ioiC ![]() 3vvmS S: Starting model for refinement C: citing same article ( |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 39475.992 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() References: UniProt: A0A0D6HE46, UniProt: A0QSZ0*PLUS, homoserine O-acetyltransferase #2: Chemical | ChemComp-GOL / | #3: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.55 Å3/Da / Density % sol: 51.8 % |
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Crystal grow | Temperature: 293 K / Method: vapor diffusion, hanging drop / pH: 5.5 Details: 32 % (v/v) polyethylene glycol (PEG) 400, 0.1 M Sodium acetate/acetic acid pH 5.5, 0.2 M calcium acetate |
-Data collection
Diffraction | Mean temperature: 100 K / Serial crystal experiment: N |
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Diffraction source | Source: ![]() ![]() |
Detector | Type: ADSC QUANTUM 270 / Detector: CCD / Date: May 10, 2017 |
Radiation | Monochromator: Double Crystal Monochromator / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.97934 Å / Relative weight: 1 |
Reflection | Resolution: 1.55→50 Å / Num. obs: 112835 / % possible obs: 97.4 % / Redundancy: 5.9 % / Rpim(I) all: 0.034 / Net I/σ(I): 39.72 |
Reflection shell | Resolution: 1.55→1.58 Å / Redundancy: 5 % / Mean I/σ(I) obs: 4.61 / Num. unique obs: 5476 / Rpim(I) all: 0.152 / % possible all: 95.4 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: 3VVM Resolution: 1.55→29.2 Å / Cor.coef. Fo:Fc: 0.965 / Cor.coef. Fo:Fc free: 0.955 / SU B: 1.288 / SU ML: 0.047 / Cross valid method: THROUGHOUT / σ(F): 0 / ESU R: 0.074 / ESU R Free: 0.074 Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS U VALUES : REFINED INDIVIDUALLY
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Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso max: 66.56 Å2 / Biso mean: 18.384 Å2 / Biso min: 9.99 Å2
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Refinement step | Cycle: final / Resolution: 1.55→29.2 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 1.546→1.586 Å / Rfactor Rfree error: 0 / Total num. of bins used: 20
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