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- PDB-6i5n: Crystal structure of SOCS2:Elongin C:Elongin B in complex with gr... -

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Basic information

Entry
Database: PDB / ID: 6i5n
TitleCrystal structure of SOCS2:Elongin C:Elongin B in complex with growth hormone receptor peptide
Components
  • Elongin-B
  • Elongin-C
  • Growth hormone receptor peptide
  • Suppressor of cytokine signaling 2
KeywordsSIGNALING PROTEIN / Complex
Function / homology
Function and homology information


JAK pathway signal transduction adaptor activity / negative regulation of receptor signaling pathway via JAK-STAT / 1-phosphatidylinositol-3-kinase regulator activity / target-directed miRNA degradation / elongin complex / VCB complex / phosphatidylinositol 3-kinase complex / growth hormone receptor binding / Cul5-RING ubiquitin ligase complex / growth hormone receptor signaling pathway ...JAK pathway signal transduction adaptor activity / negative regulation of receptor signaling pathway via JAK-STAT / 1-phosphatidylinositol-3-kinase regulator activity / target-directed miRNA degradation / elongin complex / VCB complex / phosphatidylinositol 3-kinase complex / growth hormone receptor binding / Cul5-RING ubiquitin ligase complex / growth hormone receptor signaling pathway / Cul2-RING ubiquitin ligase complex / negative regulation of multicellular organism growth / cell surface receptor signaling pathway via JAK-STAT / Pausing and recovery of Tat-mediated HIV elongation / Tat-mediated HIV elongation arrest and recovery / phosphatidylinositol phosphate biosynthetic process / HIV elongation arrest and recovery / Pausing and recovery of HIV elongation / regulation of signal transduction / Tat-mediated elongation of the HIV-1 transcript / mammary gland alveolus development / Formation of HIV-1 elongation complex containing HIV-1 Tat / Formation of HIV elongation complex in the absence of HIV Tat / RNA Polymerase II Transcription Elongation / Formation of RNA Pol II elongation complex / Growth hormone receptor signaling / cellular response to hormone stimulus / RNA Polymerase II Pre-transcription Events / Negative regulation of FLT3 / lactation / positive regulation of neuron differentiation / Interleukin-7 signaling / transcription corepressor binding / transcription elongation by RNA polymerase II / regulation of cell growth / TP53 Regulates Transcription of DNA Repair Genes / transcription initiation at RNA polymerase II promoter / Vif-mediated degradation of APOBEC3G / insulin-like growth factor receptor binding / Oxygen-dependent proline hydroxylation of Hypoxia-inducible Factor Alpha / Inactivation of CSF3 (G-CSF) signaling / Evasion by RSV of host interferon responses / Regulation of expression of SLITs and ROBOs / Antigen processing: Ubiquitination & Proteasome degradation / positive regulation of proteasomal ubiquitin-dependent protein catabolic process / response to estradiol / protein-macromolecule adaptor activity / Neddylation / ubiquitin-dependent protein catabolic process / protein-containing complex assembly / protein ubiquitination / intracellular signal transduction / ubiquitin protein ligase binding / regulation of transcription by RNA polymerase II / negative regulation of apoptotic process / nucleoplasm / cytoplasm / cytosol
Similarity search - Function
SOCS box / Suppressor of cytokine signalling 2 / SOCS2, SH2 domain / suppressors of cytokine signalling / SOCS box / SOCS box-like domain superfamily / SOCS box domain / SOCS box domain profile. / SOCS_box / Elongin C; Chain C, domain 1 ...SOCS box / Suppressor of cytokine signalling 2 / SOCS2, SH2 domain / suppressors of cytokine signalling / SOCS box / SOCS box-like domain superfamily / SOCS box domain / SOCS box domain profile. / SOCS_box / Elongin C; Chain C, domain 1 / Elongin B / Elongin-C / Potassium Channel Kv1.1; Chain A / Potassium Channel Kv1.1; Chain A / S-phase kinase-associated protein 1-like / SKP1 component, POZ domain / Skp1 family, tetramerisation domain / Found in Skp1 protein family / SKP1/BTB/POZ domain superfamily / Phosphatidylinositol 3-kinase Catalytic Subunit; Chain A, domain 1 / SH2 domain / Src homology 2 (SH2) domain profile. / Src homology 2 domains / SH2 domain / SH2 domain superfamily / Ubiquitin-like (UB roll) / Ubiquitin family / Ubiquitin homologues / Ubiquitin domain profile. / Ubiquitin-like domain / Ubiquitin-like domain superfamily / Roll / 2-Layer Sandwich / Orthogonal Bundle / Mainly Alpha / Alpha Beta
Similarity search - Domain/homology
: / Suppressor of cytokine signaling 2 / Elongin-C / Elongin-B
Similarity search - Component
Biological speciesHomo sapiens (human)
MethodX-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 1.98 Å
AuthorsKung, W.W. / Ramachandran, S. / Makukhin, N. / Bruno, E. / Ciulli, A.
Funding support United Kingdom, 3items
OrganizationGrant numberCountry
European Research CouncilERC-2012-StG-311460 DrugE3CRLs United Kingdom
Wellcome Trust100476/Z/12/Z United Kingdom
Wellcome Trust094090/Z/10/Z United Kingdom
CitationJournal: Nat Commun / Year: 2019
Title: Structural insights into substrate recognition by the SOCS2 E3 ubiquitin ligase.
Authors: Kung, W.W. / Ramachandran, S. / Makukhin, N. / Bruno, E. / Ciulli, A.
History
DepositionNov 14, 2018Deposition site: PDBE / Processing site: PDBE
Revision 1.0May 29, 2019Provider: repository / Type: Initial release
Revision 1.1Jun 12, 2019Group: Data collection / Structure summary
Category: audit_author / pdbx_seq_map_depositor_info / struct
Item: _audit_author.identifier_ORCID / _audit_author.name ..._audit_author.identifier_ORCID / _audit_author.name / _pdbx_seq_map_depositor_info.one_letter_code / _struct.title
Revision 1.2Jun 19, 2019Group: Data collection / Database references
Category: citation / citation_author ...citation / citation_author / pdbx_database_proc / pdbx_seq_map_depositor_info
Item: _citation.journal_volume / _citation.page_first ..._citation.journal_volume / _citation.page_first / _citation.page_last / _citation.pdbx_database_id_PubMed / _citation.title / _citation_author.identifier_ORCID / _citation_author.name / _pdbx_seq_map_depositor_info.one_letter_code

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Assembly

Deposited unit
A: Suppressor of cytokine signaling 2
B: Elongin-B
C: Elongin-C
D: Suppressor of cytokine signaling 2
E: Elongin-B
F: Elongin-C
J: Growth hormone receptor peptide
K: Growth hormone receptor peptide
I: Growth hormone receptor peptide
L: Growth hormone receptor peptide
hetero molecules


Theoretical massNumber of molelcules
Total (without water)89,97313
Polymers89,76010
Non-polymers2143
Water9,224512
1
A: Suppressor of cytokine signaling 2
B: Elongin-B
C: Elongin-C
K: Growth hormone receptor peptide
I: Growth hormone receptor peptide
hetero molecules


Theoretical massNumber of molelcules
Total (without water)44,9396
Polymers44,8805
Non-polymers591
Water905
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
2
D: Suppressor of cytokine signaling 2
E: Elongin-B
F: Elongin-C
J: Growth hormone receptor peptide
L: Growth hormone receptor peptide
hetero molecules


Theoretical massNumber of molelcules
Total (without water)45,0357
Polymers44,8805
Non-polymers1552
Water905
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Unit cell
Length a, b, c (Å)113.177, 156.763, 57.571
Angle α, β, γ (deg.)90.00, 90.00, 90.00
Int Tables number18
Space group name H-MP21212
Components on special symmetry positions
IDModelComponents
11E-248-

HOH

21E-266-

HOH

31E-306-

HOH

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Components

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Protein , 3 types, 6 molecules ADBECF

#1: Protein Suppressor of cytokine signaling 2 / SOCS-2 / Cytokine-inducible SH2 protein 2 / CIS-2 / STAT-induced STAT inhibitor 2 / SSI-2


Mass: 19411.959 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human) / Gene: SOCS2, CIS2, SSI2, STATI2 / Production host: Escherichia coli BL21(DE3) (bacteria) / References: UniProt: O14508
#2: Protein Elongin-B / EloB / Elongin 18 kDa subunit / RNA polymerase II transcription factor SIII subunit B / SIII p18 / ...EloB / Elongin 18 kDa subunit / RNA polymerase II transcription factor SIII subunit B / SIII p18 / Transcription elongation factor B polypeptide 2


Mass: 11852.389 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human) / Gene: ELOB, TCEB2 / Production host: Escherichia coli BL21(DE3) (bacteria) / References: UniProt: Q15370
#3: Protein Elongin-C / EloC / Elongin 15 kDa subunit / RNA polymerase II transcription factor SIII subunit C / SIII p15 / ...EloC / Elongin 15 kDa subunit / RNA polymerase II transcription factor SIII subunit C / SIII p15 / Transcription elongation factor B polypeptide 1


Mass: 10974.616 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human) / Gene: ELOC, TCEB1 / Production host: Escherichia coli BL21(DE3) (bacteria) / References: UniProt: Q15369

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Protein/peptide , 1 types, 4 molecules JKIL

#4: Protein/peptide
Growth hormone receptor peptide


Mass: 1320.407 Da / Num. of mol.: 4 / Source method: obtained synthetically / Source: (synth.) Homo sapiens (human)

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Non-polymers , 3 types, 515 molecules

#5: Chemical ChemComp-CO / COBALT (II) ION


Mass: 58.933 Da / Num. of mol.: 2 / Source method: obtained synthetically / Formula: Co
#6: Chemical ChemComp-SO4 / SULFATE ION


Mass: 96.063 Da / Num. of mol.: 1 / Source method: obtained synthetically / Formula: SO4
#7: Water ChemComp-HOH / water


Mass: 18.015 Da / Num. of mol.: 512 / Source method: isolated from a natural source / Formula: H2O

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Experimental details

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Experiment

ExperimentMethod: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

CrystalDensity Matthews: 2.85 Å3/Da / Density % sol: 56.8 %
Crystal growTemperature: 277.15 K / Method: vapor diffusion, hanging drop
Details: Sodium cacodylate, cobalt chloride, MES, ammonium sulphate

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Data collection

DiffractionMean temperature: 100 K / Serial crystal experiment: N
Diffraction sourceSource: SYNCHROTRON / Site: Diamond / Beamline: I04 / Wavelength: 0.9795 Å
DetectorType: DECTRIS PILATUS 6M-F / Detector: PIXEL / Date: Sep 24, 2017
RadiationProtocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelengthWavelength: 0.9795 Å / Relative weight: 1
ReflectionResolution: 1.98→113.55 Å / Num. obs: 73251 / % possible obs: 100 % / Redundancy: 13.3 % / Rmerge(I) obs: 0.094 / Net I/σ(I): 17.1
Reflection shellResolution: 1.98→2.01 Å

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Processing

Software
NameVersionClassification
PHENIX(dev_2420: ???)refinement
Cootmodel building
Aimlessdata scaling
PHASERphasing
RefinementMethod to determine structure: MOLECULAR REPLACEMENT / Resolution: 1.98→54.042 Å / SU ML: 0.23 / Cross valid method: NONE / σ(F): 1.33 / Phase error: 22.68
RfactorNum. reflection% reflection
Rfree0.2264 3750 2.72 %
Rwork0.1899 --
obs0.1909 72170 99.98 %
Solvent computationShrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å
Refinement stepCycle: LAST / Resolution: 1.98→54.042 Å
ProteinNucleic acidLigandSolventTotal
Num. atoms5910 0 7 512 6429
Refine LS restraints
Refine-IDTypeDev idealNumber
X-RAY DIFFRACTIONf_bond_d0.016048
X-RAY DIFFRACTIONf_angle_d1.0648201
X-RAY DIFFRACTIONf_dihedral_angle_d17.6463629
X-RAY DIFFRACTIONf_chiral_restr0.067945
X-RAY DIFFRACTIONf_plane_restr0.0061026
LS refinement shell
Resolution (Å)Rfactor RfreeNum. reflection RfreeRfactor RworkNum. reflection RworkRefine-ID% reflection obs (%)
1.98-2.00510.31141420.27664906X-RAY DIFFRACTION100
2.0051-2.03140.26421350.26714957X-RAY DIFFRACTION100
2.0314-2.05930.26191420.2495023X-RAY DIFFRACTION100
2.0593-2.08870.27611380.23224932X-RAY DIFFRACTION100
2.0887-2.11990.23641340.23144955X-RAY DIFFRACTION100
2.1199-2.1530.22291340.22044981X-RAY DIFFRACTION100
2.153-2.18830.24121420.21534921X-RAY DIFFRACTION100
2.1883-2.2260.25121400.21445034X-RAY DIFFRACTION100
2.226-2.26650.24551340.2194926X-RAY DIFFRACTION100
2.2665-2.31010.2271420.19874997X-RAY DIFFRACTION100
2.3101-2.35730.25791400.19644928X-RAY DIFFRACTION100
2.3573-2.40850.23521380.19654994X-RAY DIFFRACTION100
2.4085-2.46450.30421380.19914914X-RAY DIFFRACTION100
2.4645-2.52620.2641380.19625006X-RAY DIFFRACTION100
2.5262-2.59450.29711410.19714961X-RAY DIFFRACTION100
2.5945-2.67080.27031410.20024884X-RAY DIFFRACTION100
2.6708-2.7570.28871410.21335063X-RAY DIFFRACTION100
2.757-2.85560.30971380.20634912X-RAY DIFFRACTION100
2.8556-2.96990.25151350.20014957X-RAY DIFFRACTION100
2.9699-3.1050.22371400.20384989X-RAY DIFFRACTION100
3.105-3.26870.26041430.19584958X-RAY DIFFRACTION100
3.2687-3.47350.18971410.17664953X-RAY DIFFRACTION100
3.4735-3.74160.22351370.16994979X-RAY DIFFRACTION100
3.7416-4.1180.18731410.1494991X-RAY DIFFRACTION100
4.118-4.71360.14591390.13234943X-RAY DIFFRACTION100
4.7136-5.93750.1571390.17064963X-RAY DIFFRACTION100
5.9375-54.0620.20211370.19314960X-RAY DIFFRACTION100
Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

IDL11 (°2)L12 (°2)L13 (°2)L22 (°2)L23 (°2)L33 (°2)S11 (Å °)S12 (Å °)S13 (Å °)S21 (Å °)S22 (Å °)S23 (Å °)S31 (Å °)S32 (Å °)S33 (Å °)T11 (Å2)T12 (Å2)T13 (Å2)T22 (Å2)T23 (Å2)T33 (Å2)Origin x (Å)Origin y (Å)Origin z (Å)
11.51190.4658-0.51261.9898-2.12847.41790.1574-0.05460.04350.12050.13480.3245-0.0015-0.2282-0.2770.1167-0.0417-0.01630.24440.01020.2385-16.065931.0492-26.5836
21.71560.28-0.12388.81890.81795.8127-0.09281.12790.2075-0.8070.1396-0.6741-0.2660.7288-0.00260.2529-0.0180.02010.61660.09350.2357-4.002237.3208-43.2955
34.83071.0659-1.26952.27550.78443.6177-0.12060.2547-0.4494-0.0789-0.1401-0.32930.1620.43770.2670.1004-0.01510.01030.26340.03560.2294-3.499131.6673-31.4966
44.50450.4166-0.24350.04230.05270.0895-0.0669-0.13790.34510.18760.1542-0.09430.2343-0.0251-0.10.20810.0267-0.0130.27610.02040.31380.397634.7824-18.3074
53.014-0.26021.13322.5285-4.7329.5801-0.24350.08350.05410.4311-0.0831-0.296-0.4120.1610.35910.1733-0.0216-0.00770.2303-0.01060.2148-8.849240.9339-22.4256
65.5897-0.98711.09460.4173-0.04540.9304-0.1040.27550.46160.0386-0.1167-0.2577-0.24020.29020.16120.2329-0.0542-0.01230.270.11980.2861-6.867844.3232-33.6788
73.3488-0.4483-0.54211.97472.56796.660.005-0.2690.0506-0.0211-0.0201-0.1157-0.41260.18020.0090.1884-0.0096-0.02130.19820.04040.1381-26.51248.702-26.4882
83.4693-2.305-4.74384.34031.46898.5085-0.17-0.7844-0.75110.3336-0.01180.07190.3010.11430.12490.1632-0.0194-0.03450.24930.05580.1984-25.972243.0007-23.4673
97.0338-4.11163.54667.6102-3.22773.76590.10050.4442-0.3291-0.1745-0.24510.1337-0.19750.45540.07570.2481-0.0528-0.01830.26130.00380.1123-30.471150.122-55.849
104.0244-1.3456-1.98373.0407-0.30016.5766-0.12870.10340.01350.0146-0.04340.0705-0.26030.35120.19280.1962-0.0601-0.04880.16-0.01670.1062-32.683649.6328-57.5305
118.25920.2416-4.15882.1678-2.65247.3422-0.03040.4292-0.1767-0.57420.11470.25260.1172-0.1851-0.07720.2693-0.0401-0.05320.1977-0.01660.1394-36.792447.9838-65.4533
128.80131.74110.95328.31810.17256.16850.0090.64760.3897-0.46530.06030.2654-0.3032-0.4571-0.05180.28150.0179-0.01450.21140.04590.1364-40.768754.6801-66.2184
137.7125-1.71630.29055.50372.28015.1925-0.00550.22170.6844-0.1650.0264-0.2539-1.3950.6922-0.03930.4933-0.1823-0.02580.35410.04850.2352-27.587758.3392-64.9622
140.50241.0631-1.08234.1188-1.30994.0555-0.11380.24160.12350.2413-0.1942-0.3049-0.51860.94630.35960.2829-0.0811-0.01610.2827-0.00050.1416-27.354353.0807-55.0638
158.5274-3.27474.27185.1057-4.2454.04290.11731.49450.7408-1.26640.26470.9287-0.4658-1.9174-0.29720.68590.1632-0.05810.70910.13330.4631-47.767961.0311-69.0353
160.75160.113-0.00471.8667-3.34816.61520.03670.02810.24490.16170.19060.3988-1.5701-0.9951-0.21310.59490.0502-0.02180.18650.03430.308-40.63461.4143-52.6687
174.6702-0.1851-0.43480.4869-0.62190.8903-0.6801-1.58820.8620.86840.7806-0.87470.25910.7735-1.06481.6502-0.3851-0.3251.2036-0.40430.433-30.098760.1502-27.6413
183.4184-0.1549-0.32354.7544-2.03355.6265-0.02860.0573-0.0904-0.31680.01120.05380.2404-0.17460.03720.1138-0.0131-0.01580.1232-0.03590.149-40.272641.8278-46.8065
194.99943.95061.11153.2861.10230.7352-0.08550.01310.26550.2682-0.23331.30750.2857-0.32680.34330.4403-0.04390.08240.3446-0.14410.4714-52.059131.6199-47.7787
206.3132-0.7053-0.55282.56130.36554.12660.05950.1657-0.18730.0412-0.1275-0.00490.00870.11740.05650.1481-0.0139-0.0140.130.02330.0851-30.400344.918-43.0994
214.04973.84621.40053.86821.97326.3135-0.1116-0.2004-0.0647-0.09010.0922-0.0985-0.3525-0.27090.08150.16710.02260.00750.18590.00240.1787-33.552847.3452-33.2031
224.67184.8871-3.966.0163-4.61993.56-0.02290.06190.0217-0.17260.14590.3070.0918-0.4126-0.12580.1351-0.0134-0.04240.24310.03230.2876-20.930121.9262-81.4713
231.5468-1.1762-0.10911.99030.8421.52110.1851-0.8265-0.31910.3876-0.3214-0.58330.10790.74560.15340.2546-0.0717-0.08960.51120.20450.424-12.903314.5362-64.6757
242.6421-1.21042.00954.7937-0.28112.6876-0.1759-0.15-0.6938-0.3299-0.1853-0.89730.32890.43530.16430.21610.0570.06530.31580.14570.4954-13.24249.7906-76.1165
250.96250.67661.87091.28382.42135.1118-0.3010.1053-0.9191-0.184-0.4976-0.52231.10190.73080.57710.57350.06950.1120.43410.27860.8604-16.6589-0.364-70.4772
263.2831-0.46260.8061.35390.2966.79440.04290.1498-0.0576-0.0829-0.1502-0.0533-0.21470.10990.11590.1997-0.06310.02430.14180.02110.1558-35.344112.9615-75.1813
275.79771.4488-1.6064.6455-0.31223.67770.04930.1953-0.1390.1698-0.103-0.2641-0.12010.54080.09920.2394-0.07740.00580.16060.03510.0945-42.259710.9436-46.4135
281.48420.12670.42231.5677-0.72053.55140.0744-0.0460.19770.1343-0.15990.004-0.33190.50590.10110.2424-0.09250.01640.1961-0.03920.1353-43.660412.633-44.7558
293.38920.1322.08212.1555-2.03323.37180.0743-0.43470.4650.78360.09090.0115-1.15040.20850.08120.4069-0.1268-0.00430.1943-0.07470.1628-46.901815.6758-36.9012
308.2824-3.63670.00624.58372.36634.7095-0.0534-0.36230.02120.5570.07660.2562-0.2911-0.01650.04720.3109-0.06880.0610.148-0.0120.1807-53.344411.2764-36.113
312.43173.9962.35537.06634.39922.8762-0.0218-0.0088-0.12260.324-0.0382-0.17340.09270.2749-0.05360.2815-0.0709-0.00120.2437-0.01410.1859-45.34461.5929-37.5828
326.9793-1.35282.74782.46621.33422.6695-0.17370.3371-0.0053-0.107-0.2398-0.27310.33770.6230.31310.2754-0.02540.01160.3037-0.00710.2344-36.01094.4575-40.9055
332.47161.3633-1.0091.4454-0.04463.1044-0.171-0.10950.0705-0.02090.08210.00050.11760.29150.11720.2777-0.0521-0.02740.15650.02660.1363-42.81637.9993-48.1519
342.1571-2.6628-0.14065.56592.1093.80991.0532-1.65321.3421.5498-0.54961.2348-0.6208-1.5197-0.29520.5688-0.07050.09510.5428-0.11640.394-62.70388.3271-32.7592
354.18450.9544-5.35820.2744-1.55268.6282-0.22270.2794-0.2379-0.28440.18320.23390.7109-0.68370.1120.3364-0.09-0.01610.1682-0.01520.2242-56.11195.0088-49.6587
360.9133-0.9208-1.08670.95251.40065.0930.01120.9663-0.6339-0.7471-0.3303-0.0490.4686-0.0839-0.2561.2606-0.18490.31680.7185-0.34340.4201-48.52991.5778-73.3486
373.39991.077-0.51132.91640.79382.15430.1844-0.00830.2765-0.0854-0.11140.0028-0.4728-0.0447-0.03160.3632-0.05140.03060.1358-0.00690.2012-47.715723.2106-55.6912
382.40522.77731.27495.63442.16971.2086-0.1764-0.04840.5843-0.18790.03470.6033-0.10340.08520.15060.32530.0089-0.01890.2649-0.01110.3233-50.39129.7435-59.3375
394.33-0.8252-0.06882.126-0.68413.96480.0862-0.12650.07970.0736-0.1819-0.2244-0.13690.27260.09160.2618-0.09340.00040.14830.0310.1471-38.545214.516-62.1263
402.31660.6764-0.00494.59560.41071.9953-0.010.86650.1212-0.25720.2025-0.8148-0.15250.6196-0.18010.1447-0.03590.00220.4060.02320.38855.153435.3674-31.9827
412.1378-1.19180.59092.8227-1.56362.08360.4503-0.4667-0.69940.4959-0.0816-1.03040.68130.3844-0.3430.38240.0637-0.11380.61590.22810.815-4.79175.3651-69.4654
425.7433-0.4152-1.27531.83650.01182.5732-0.06750.0611.46650.18020.152-0.5896-0.50360.15570.02350.2942-0.0956-0.10130.3240.07470.66271.652347.922-24.968
430.36010.70160.37091.54350.65270.85210.3940.0873-0.5792-0.72850.2727-0.33720.2412-0.013-0.05660.66530.1257-0.06520.51560.21711.3765-13.5171-3.4018-75.7823
Refinement TLS group
IDRefine-IDRefine TLS-IDSelection details
1X-RAY DIFFRACTION1chain 'A' and (resid 30 through 54 )
2X-RAY DIFFRACTION2chain 'A' and (resid 55 through 69 )
3X-RAY DIFFRACTION3chain 'A' and (resid 70 through 100 )
4X-RAY DIFFRACTION4chain 'A' and (resid 101 through 115 )
5X-RAY DIFFRACTION5chain 'A' and (resid 116 through 131 )
6X-RAY DIFFRACTION6chain 'A' and (resid 132 through 162 )
7X-RAY DIFFRACTION7chain 'A' and (resid 163 through 184 )
8X-RAY DIFFRACTION8chain 'A' and (resid 185 through 198 )
9X-RAY DIFFRACTION9chain 'B' and (resid 1 through 9 )
10X-RAY DIFFRACTION10chain 'B' and (resid 10 through 23 )
11X-RAY DIFFRACTION11chain 'B' and (resid 24 through 35 )
12X-RAY DIFFRACTION12chain 'B' and (resid 36 through 45 )
13X-RAY DIFFRACTION13chain 'B' and (resid 46 through 60 )
14X-RAY DIFFRACTION14chain 'B' and (resid 61 through 79 )
15X-RAY DIFFRACTION15chain 'B' and (resid 80 through 84 )
16X-RAY DIFFRACTION16chain 'B' and (resid 85 through 95 )
17X-RAY DIFFRACTION17chain 'B' and (resid 96 through 103 )
18X-RAY DIFFRACTION18chain 'C' and (resid 16 through 46 )
19X-RAY DIFFRACTION19chain 'C' and (resid 47 through 58 )
20X-RAY DIFFRACTION20chain 'C' and (resid 59 through 96 )
21X-RAY DIFFRACTION21chain 'C' and (resid 97 through 112 )
22X-RAY DIFFRACTION22chain 'D' and (resid 30 through 46 )
23X-RAY DIFFRACTION23chain 'D' and (resid 47 through 81 )
24X-RAY DIFFRACTION24chain 'D' and (resid 82 through 131 )
25X-RAY DIFFRACTION25chain 'D' and (resid 132 through 154 )
26X-RAY DIFFRACTION26chain 'D' and (resid 155 through 198 )
27X-RAY DIFFRACTION27chain 'E' and (resid 1 through 9 )
28X-RAY DIFFRACTION28chain 'E' and (resid 10 through 23 )
29X-RAY DIFFRACTION29chain 'E' and (resid 24 through 35 )
30X-RAY DIFFRACTION30chain 'E' and (resid 36 through 45 )
31X-RAY DIFFRACTION31chain 'E' and (resid 46 through 55 )
32X-RAY DIFFRACTION32chain 'E' and (resid 56 through 65 )
33X-RAY DIFFRACTION33chain 'E' and (resid 66 through 79 )
34X-RAY DIFFRACTION34chain 'E' and (resid 80 through 84 )
35X-RAY DIFFRACTION35chain 'E' and (resid 85 through 95 )
36X-RAY DIFFRACTION36chain 'E' and (resid 96 through 101 )
37X-RAY DIFFRACTION37chain 'F' and (resid 16 through 47 )
38X-RAY DIFFRACTION38chain 'F' and (resid 48 through 66 )
39X-RAY DIFFRACTION39chain 'F' and (resid 67 through 112 )
40X-RAY DIFFRACTION40chain 'I' and (resid -4 through 5 )
41X-RAY DIFFRACTION41chain 'J' and (resid -4 through 5 )
42X-RAY DIFFRACTION42chain 'K' and (resid -3 through 5 )
43X-RAY DIFFRACTION43chain 'L' and (resid -2 through 5 )

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